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Citations for Worthington Biochemical :
251 - 300 of 432 citations for 2 Methoxycarbonyl 4 methylthiophene 3 diazonium tetrafluoroborate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The EBs were dissociated by incubation in Collagenase type 2 (0.5 mg/mL, Worthington) in HANKs buffer overnight at room temperature followed by TrypLE for 5 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Right lung lobes were minced and digested with 5 mL digestion buffer: Collagenase 4 (200U/mL, Worthington Biochemicals), DNase I (100 ug/mL ...
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bioRxiv - Immunology 2021Quote: ... Cells were then DNAse treated with 4 ml dissagregation media (DMEM, 10% FBS, 100 μg/ml DNAse (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... and they were further digested for 30 min in 4 mg/ml collagenase II (Worthington, cat. No. LS004176) and 4.66 mg/ml dispase II (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and the telencephalon was manually cut into pieces, followed by enzymatic digestion (DMEM GlutaMAX 1% P/S, 3% papain (Worthington 3126), 1.5% 10 mg ml−1 DNAse and 2.4% 12 mg ml−1 cystein ...
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bioRxiv - Immunology 2020Quote: ... inflated with a 1.5 ml mixture of 1 ml low melting agarose (3% w/v) and 500 μl 4U/ml dispase (Worthington Biochem, LS02104), and incubated for 1 h at room temperature (RT) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... enzymatic digestion was achieved by 3 min of perfusion with calcium-free digestion buffer (400 units/mL of collagenase II in perfusion buffer; Worthington LS004177) followed by 12 min of perfusion with digestion buffer containing 50 μM CaCl2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Biophysics 2022Quote: ... CaCl2 was added to a final concentration of 40 mM (21 µL of 1 M) followed by the addition of 75 U (3 µL of 25 U/µL) of micrococcal nuclease (MNase, Worthington Biochemical) and incubated at 37◦C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue was then washed with PBS and digested for 10 min at 37°C in 3 mg/ml collagenase II (Worthington, LS004176) in HBSS supplemented with 5% FBS and 10 mM HEPES ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2024Quote: ... Excised hearts were cannulated to the Langendorff retrograde perfusion system through the aorta and perfused with Base solution (Tyrode’s solution with 10 mM taurine and 12 mM 2,3-butanedione monoxime) and then digested with prewarmed Digestion buffer (Base solution with 180 U/mL Collagenase Type II (Worthington, LS004177) and 25 μM CaCl2 ...
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bioRxiv - Immunology 2022Quote: ... biopsies were minced finely with dissection scissors and mixed with 3mL of digestion buffer (0.8mg/mL collagenase type 4 (Worthington) and 0.02mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mL of Ca2+/Mg2+-free HBSS containing 4 mg of collagenase type II and 1.25 mg of Dispase type II (Worthington) was added and incubated at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... for 4 minutes at a flow rate of 5ml/min followed by 0.8mg/ml Type 1 Collagenase (Worthington, UK) in Dulbecco Minimum Essential Medium (Gibco # 11965-092 ...
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Pulmonary infection interrupts acute cutaneous wound healing through disruption of chemokine signalsbioRxiv - Immunology 2020Quote: ... The tissue was incubated for 45 minutes at 37° C in DMEM containing type 4 collagenase (Worthington Biochemical Corporation) and DNAse I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated overnight at 4°C with 15μl purified proteins at optimized concentrations in 0.1M Sodium Bicarbonate: DNA (Worthington, LS002105) at 5μg/mL ...
Product cited:
Highly polymerized; hyperchromicity ≥27%. A substrate for deoxyribonuclease assays. Prepared...Cat# LS002105, 100 mg, $35.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... lungs were perfused with 5 ml PBS and then digested with 4 U/ml elastase (Worthington Biochemical Corporation, NJ) and 100 U/ml DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Ganglia were placed into a digestion buffer containing 1 mg/ml (325 U/ml) collagenase type 4 (Worthington LS004189), 2 mg/ml (1.8 U/ml ...
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bioRxiv - Immunology 2024Quote: ... and skin was cut into small pieces and mixed with digestion buffer containing 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
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bioRxiv - Immunology 2021Quote: ... mechanically cut into small pieces and digested with 0,1 % Collagenase type 2 (265U/mL; Worthington) and DNase I (250U/mL ...
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bioRxiv - Cell Biology 2020Quote: ... minced lung tissue was transferred to 25 mL pre-warmed collagenase (2 mg/mL, Worthington Biochemical Corporation ...
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bioRxiv - Bioengineering 2021Quote: ... followed by 2 h digestion with 300 U ml-1 type I collagenase (Worthington Biochemical). Dissociated cells were seeded in culture plates ...
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bioRxiv - Developmental Biology 2022Quote: ... were incubated in 2 mg/ml collagenase type II (260 U/mg, Worthington, Lakewood, NJ) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The perfusate was then switched to Tyrode’s solution containing collagen type 2 (Worthington, Columbus, OH) and protease type 14 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... 200 μL of 20 mg/mL of type 2 collagenase in L15 (Cat.No. NC9870009; Worthington) were added and incubated on an orbital rotator at 28°C for 35 min ...
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bioRxiv - Bioengineering 2024Quote: ... leaflets were digested in EBSS containing 250 units of Collagenase type 2 (Worthington Biochemical, LS004176) for 30 minutes at 37°C under constant agitation at 100 rpm ...
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bioRxiv - Developmental Biology 2024Quote: ... and digested in Leibovitz’s with 2 mg/mL Collagenase Type I (Worthington, CLS-1, LS004197), 2 mg/mL Elastase (Worthington ...
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bioRxiv - Cell Biology 2024Quote: ... Day 10-50 EBs were dissociated using Collagenase type 2 (1 mgl/ml, Worthington, 4176) in HANKs buffer for 16 hours at room temperature ...
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bioRxiv - Physiology 2024Quote: ... and digested with pre-warmed collagenase media (500 U/mL collagenase type 2 (LS004183, Worthington) and 3.75U/mL DNase I (10104159001 ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
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bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was collected by centrifuged at 300 g for 5 min and then resuspended in 3 mL Ovomucoid protease inhibitor with FBS (Inhibitor-BSA Vial [LK003182], Worthington, Lakewood, NJ). To remove the debris ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... the RPE cells were washed at least 3 times with PBS to remove the dead cells and dissociated using Papain (Worthington Biochemical Corporation) for 30mins-1h at 37 °C following the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... at 4°C followed by eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (1mg/ml Worthington) at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS and dissociated into single cells using collagenase type 4 enzyme at 200Units/ml (Worthington, Lakewood, NJ, US) for 10min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and enzymatically digested at 37°C for 80 min in dispase type II (4.7 mg/ml) plus collagenase type II (4 mg/ml) (Worthington Biochemical), as described previously [62] ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA was then digested for 2 hr at 37 °C in 1/10 volume of 1 M ammonium bicarbonate with snake venom phosphodiesterase (1.2×10−4 U/μg RNA) and bacterial alkaline phosphatase (0.1U/ μg RNA) (Worthington Biochemical corporation). Analysis was performed on a TSQ Quantiva Triple Quadrupole (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... After adding 0.9 mL pre-warmed HBSS containing 10 mg/mL Collagenase Type 2 (Worthington, UK) samples were incubated for 25 min at 37°C rotating every 5 min and then washed with 2 mL pre-warmed DMEM/F12 supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... and incubated in 2 ml enzymatic solution containing 1.5 mg/ml collagenase type IV (Worthington, LS004188) and 0.5 mg/ml Dnase I (Roche ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... skin was minced and incubated in sterile 0.5% Collagenase Type 2 (Worthington Chemicals, Cat. No. LS004176) overnight at 37 °C in an atmosphere of 5% CO2 and 5% O2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... The digestion was terminated with the addition of 2 ml Ovomucoid (0.7 mg/ml, Worthington, LS003086). Following digestion ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003086, 2 gm, $280.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... HBSS was removed and 2 ml of freshly made 1 mg/ml collagenase (Worthington Biochemical, LS004194) in HBSS was added to each tube ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Immunology 2021Quote: ... MLV-SARS-CoV-2 pseudovirus was activated with 10 µg/ml TPCK treated-Trypsin (Worthington Biochem) for 1 h at 37 °C ...
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bioRxiv - Genetics 2022Quote: ... digested for 30 minutes with 245 units/ml type-2 collagenase (Worthington Biochemical Corp, Lakewood, USA) and 96 μg/ml soybean trypsin inhibitor (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Single-cell suspensions were prepared by using Papain containing l-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3 ...
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bioRxiv - Cell Biology 2023Quote: ... and transferred into a digestion buffer consisting of 2 mg/mL collagenase II (LS004176, Worthington Biochemical) and 0.3 mM calcium chloride in CIB ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2022Quote: ... The digestion was terminated with the addition of 2 ml Ovomucoid (0.7 mg/ml, Worthington, LS003086). Following digestion ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003086, 2 gm, $280.00 Ask