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Citations for Worthington Biochemical :
101 - 150 of 220 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the cells were dissociated using TryplE (Thermo Fischer Scientific) with added DNaseI (Worthington) and plated onto plates previously coated with Matrigel (BD Bioscences ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were collected using collagenase type I (1mg/mL in PBS, Worthington Biochemical) and passed through a 40 µm cell strainer before use in subsequent assays.
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bioRxiv - Genomics 2024Quote: ... and dissociated to single cell suspension using the Papain Dissociation System (Worthington #LK003150) following manufacturer’s protocol without the use of ovomucoid protease inhibitor ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... Single cell suspensions were generated by enzymatic digestion (Collagenase IV, Invitrogen and Elastase, Worthington) for scRNA-seq library preparation ...
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bioRxiv - Genetics 2020Quote: ... cells were dissociated from transwells with a papain dissociation kit (Worthington Biochemical, Cat# LK003150) and filtered using a Flowmi cell strainer (Bel-Art SP Scienceware ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... iMNs were dissociated into single cells using the Papain Dissociation System (Worthington Biochemical, LK003150) after collection at Day 6 and resuspended to the concentration of 0.2 × 106 cells/mL with MN medium consisting of Neurobasal medium (Gibco 21103049 ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... Stromal cells were released by treatment with 3 mg/ml Type I Collagenase (Worthington) in 2 ml HBSS for 1 hr at 37°C with shaking at 110 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... Organoids were dissociated into single cells by using the Papain dissociation system (Worthington Biochemical) as previously described [96] ...
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bioRxiv - Genomics 2021Quote: ... and treated each T cell culture with 200U/mL of DNase (Worthington Cat # LS002007) for 30 mins at 37 degrees ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... the cell dissociation protocol was carried out using the Papain Dissociation System (Worthington, LK003150), following the manufacturer’s protocol ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Genetics 2020Quote: ... the cells were passaged with Accutase containing 20 units/mL of papain (Worthington, LK00031765) and plated at 3.5 x 105 cells per cm2 on 0.01% Geltrex-coated plates for final maturation.
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bioRxiv - Neuroscience 2022Quote: ... Dissected tissue was then dissociated into cell suspension62 using the Papain dissociation system (Worthington) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Bone marrow cells were then incubated with 0.1U·mL-1 Collagenase (Worthington Biomedical Corp., #LS004176), 0.8U·mL-1 Dispase (Worthington Biomedical Corp. ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Genetics 2023Quote: ... cells were enzymatically extracted from Matriderm by adding collagenase (2000 IU/ml, Worthington, LS004176) in DMEM with 10% FCS and 1% P/S and shaking at 37°C for 3 hours ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2023Quote: ... the tumor cells were dissociated with collagenase type IV and I (Worthington Biochemical Co.) for 20 min at 37°C and washed with PBS containing 1% FBS and 0.1% sodium azide (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Bone stromal cells were obtained by treating the crushed bone with collagenase type 1 (Worthington) enzyme (3mg/mL ...
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bioRxiv - Genomics 2019Quote: ... Dissociated cells were layered on the top of 5 ml of Ovomucoid inhibitor solution (Worthington) and harvested by centrifugation (140 x g for 6 min) ...
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bioRxiv - Developmental Biology 2019Quote: Cells were trypsinized and at T14 or later also treated with collagenase type 1 (Worthington) for 15-30 min at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... single cells were prepared from the bulk tumor by a Papain dissociation kit (Worthington Biochemical) as per the manufacturer’s protocol and cultured using previously reported culturing methods76 ...
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bioRxiv - Bioengineering 2020Quote: ... E16.5 embryonic cortices into single cell suspensions with a papain dissociation system (Worthington Biochemical Corporation). Tissue culture plates were coated with poly-L-lysine (0.1% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... The MOE tissue was dissected and cells were dissociated using Papain Dissociation System (Worthington Biochemical) following manufacturer’s instructions with minor modifications ...
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bioRxiv - Cell Biology 2021Quote: ... cardiac cells were dissociated by enzymatic digestion with 0.04% collagenase II (Worthington, Lakewood, NJ, USA) and 0.05% pancreatin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... total glioma cells were isolated from GBM tumors with the Papain Dissociation System (Worthington Biochemical) according to the protocol provided by the manufacturer ...
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bioRxiv - Genetics 2022Quote: Organoids were dissociated into single-cell suspension with the Papain dissociation system (Worthington Biochemical, LK003150). Cells were then loaded onto the Chromium Next GEM Chip G Single Cell Kit ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... The dissociated cells were layered on top of 5 ml of ovomucoid inhibitor solution (Worthington) and harvested by centrifugation (70 x g for 6 min) ...
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bioRxiv - Physiology 2023Quote: ... a single cell suspension was obtained through enzymatic digestion with elastase (Worthington, Lakewood, NJ, USA) and liberase ((Roche ...
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bioRxiv - Physiology 2024Quote: ... Cells were enzymatically dissociated using a cocktail of collagenase type II (210 U/ml, Worthington), collagenase type IV (260 U/ml ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: Retinas were dissociated into single-cell suspensions using a papain dissociation kit (Worthington, Columbus, OH). Briefly ...
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bioRxiv - Genomics 2024Quote: Single cell dissociation was performed using Papain and Deoxyribonuclease I (Worthington Biochemical Cat. no. LK003153) for 20 min at 37°C and stopped using Earl’s balanced salt solution (EBSS-Thermo Fisher Scientific ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003153, 3 bx, $775.00 Ask -
bioRxiv - Bioengineering 2019Quote: ... Cell seeded hydrogels were degraded with 100 Units of Collagenase Type IV (Worthington Biochemical, Lakewood, NJ) for 20 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... glial cell line-derived neurotrophic factor (GDNF) and fetal bovine serum (FBS) were purchased from Worthington Biochemical Corporation (Lakewood ...
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bioRxiv - Systems Biology 2021Quote: To prepare single cell suspensions biopsies were minced and digested using Collagenase II (Worthington Biochemical Corporation) and DNase (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... EBSS was removed and individual cells were dissociated from the tissue in 100 µl Papain (Worthington, #LK03178 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were dispersed and incubated with Ovo-mucoid protease inhibitor (Worthington Biochemical, cat. no. LK003182) to inactivate residual papain activity by incubating on a rocker at 4 °C for 20 min ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Developmental Biology 2019Quote: ... Tissue was dissociated into a single-cell suspension using papain according to the manufacturer’s instructions (Worthington), and resuspended in cortico-spinal motor neuron (CSMN ...
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bioRxiv - Plant Biology 2020Quote: ... The unfertilized ovules of each cell marker line were treated with enzyme solution (1 % cellulase [Worthington, USA] ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then dissociated by tissue trituration and papain treatment [20U/ml papain (Worthington Biochemical corporation), and 0.2 mg/ml L-cysteine (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Single cell dissociation was performed by incubating tissue in papain (1 mg/ml; Worthington Biochemical Co) plus 1% DNaseI (10mg/ml in HBSS ...
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bioRxiv - Neuroscience 2022Quote: ... Single-cell suspensions were prepared by using Papain containing l-cysteine and EDTA (vial 2, Worthington), DNase I (vial 3 ...
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bioRxiv - Immunology 2023Quote: ... NP-40 cell lysates were incubated with 100 μg/mL of DNase I (Worthington Biochemical, LS002139) or 50 μg/mL of RNase A (Worthington Biochemical ...
Product cited:
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bioRxiv - Neuroscience 2023Quote: ... Cortices were then dissociated into single cells via incubation in 20 U/mL Papain/DNase (Worthington) for 15-20 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Cortical tissue was dissociated into cells using 200u/ml papain (cat # LS003126, Worthington, Columbus, Ohio, USA) and DNAse followed by trituration ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated for 7 min with Neurobasal media containing Ovomucoïde (15 mg/mL, Worthington), and manually triturated 10 times with a 1000 ml pipette in OptiMeM supplemented with D-Glucose (20mM) ...
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bioRxiv - Developmental Biology 2024Quote: Cerebral organoids were dissociated into a single-cell suspension using Worthington Papain System kit (Worthington, LK003150) according to the manufacturer’s instruction ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 5 minutes at RT in fetal calf serum complemented with DNase I (Worthington), followed by mechanical dissociation ...
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bioRxiv - Neuroscience 2022Quote: ... and the cells dissociated for 30 min at 37 °C with 0.2% collagenase type III (Worthington LS004182) in Hibernate-A medium (Thermo A1247501) ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2022Quote: ... Cells were pelleted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DNaseI (Worthington Papain system ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pelted 200g 5min at 4°C and resuspended in 200μl aCSF with 2.5% DnaseI (Worthington Papain system ...
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bioRxiv - Immunology 2023Quote: ... the cells of the lymph nodes were dissociated with collagenase type IV (Worthington Biochemical Co., NJ, USA) for 20 min at 37°C ...
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bioRxiv - Genomics 2024Quote: ... Harvested tumors were minced and dissociated to single cell suspension using the Papain Dissociation System (Worthington #LK003150) following manufacturer’s protocol without the use of ovomucoid protease inhibitor ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask