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Citations for Worthington Biochemical :
651 - 694 of 694 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The ganglia were placed into a digestion buffer composed of 1 mg/ml (325 U/ml) collagenase type 4 (Worthington LS004189), 2 mg/ml (1.8 U/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Terminal differentiation of oligodendrocytes was achieved in oligodendrocyte differentiation media by mitogen withdrawal for 7 days (except for T3 and IGF-1) following single-cell dissociation using Papain (20 units/ml) dissociation kit (Worthington Biochemical) and plating on Matrigel (SLS ...
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bioRxiv - Developmental Biology 2024Quote: ... minced vessels were incubated for 60 minutes at 37°C with agitation in an enzymatic cocktail consisting of 1 mg/ml collagenase I (Worthington, LS004196), 1 mg/ml Dispase II (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... The cardiac ventricles were cut into pieces of approximately 1–2 mm3 and dissociated by trituration in 0.05% collagenase type II (Worthington, Lakewood, NJ, USA) 8-10 times for 5 min each ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... The collected nerve segments were cut into small pieces with microscissors and incubated in 1 ml collagenase (4mg/ml Worthington Biochemical, LS004176) and dispase (2mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Genomics 2021Quote: ... Lung tumor sample were cut into small pieces with a razor blade and enzymatically digested (100 U/mL Collagenase IV, Sigma-Aldrich, C5138-1G; 50 µg/mL DNase 1, Worthington, LS002138) for 35 minutes being rotated at 37°C in HEPES buffered RPMI 1640 containing 0.5% FBS ...
Product cited:
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bioRxiv - Neuroscience 2020Quote: ... The collected nerve segments were cut into small pieces with microscissers and incubated in 1 ml PBS supplemented with collagenase (4mg/ml Worthington Biochemical, LS004176), dispase (2mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2020Quote: ... the heart was perfused with the same solutions with 50 μM Ca2+ and collagenase type II (1 mg/mL, CLS-2, Worthington, NJ, USA) for 15 minutes ...
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bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
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bioRxiv - Immunology 2021Quote: ... tissue was finely minced and placed into digestion media (RPMI basal media, 300 U/mL collagenase I [Worthington Biochemical Corporation], 1% BCS), shaken for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and cut into rings (about 1 mm in length) that were subsequently incubated in 2 mg/ml collagenase type II (Worthington Biochemical Corporation) for 2 hr ...
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bioRxiv - Cell Biology 2023Quote: Tissue samples were dissected into small pieces and digested with 1-2 mg/ml (275 U/ml) collagenase type II (Worthington, Columbus, USA) in 30 ml Dulbecco’s minimal essential medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: The primary neuronal cells were isolated from the brain tissues of 1∼2-day-old SD pups using the papain dissociation system (Worthington Biochemical Corporation) as instructed by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... for 5 min at 4°C to remove circulating cells and then digested in RPMI 1640 with 1 mg/mL Type 2 collagenase (Worthington Biochemical LS004177) for 45 min at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2024Quote: Neuronal cells were extracted from the brains of 1-day-pold SD pups using a papain dissociation system (Worthington Biochemical Corporation, Worthington, NJ, USA) following the manufacture’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... Two to three pieces of 0.5g testis tissue was placed in a 50mL Falcon tube with 10mL prewarmed collagenase solution at 32 °C (1 mg/mL collagenase I (Worthington Biochemicals #LS004196), 1 mM EDTA ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred to a 15ml tube with 8ml collagenase solution (∼4,000 units in αMEM with 1% anti-mycotic; Type IV, Worthington Biochemical Corp, Lakewood, NJ). The solution containing the tissue fragments was then continuously agitated for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Homogenised tissue was added to a solution (total volume of 7 ml) composed by of 1 mg/ml collagenase IV (Worthington Biochemical Corp, USA), 0.02 mg/ml DNase 1 (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
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bioRxiv - Microbiology 2022Quote: ... of 0.01 and maintained in MEM containing 0.3% bovine serum albumin (MEM/BSA) and 1 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-trypsin (Worthington, Lakewood, NJ, USA). Supernatants were collected every 12 h and viral titers were measured using a plaque assay.
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bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue fragments were incubated in 1.35 mL PBS plus 150 uL 20 mg/mL Collagenase Type I (Worthington CLS-1 dissolved in PBS) and 15 uL 20 mg/ml DNase I (Worthington D dissolved in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Brain tissues were manually minced using razor blades and enzymatically dissociated at 37°C for 30 min with 1 mg/mL collagenase type IV (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/mL DNase 1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Paleontology 2023Quote: ... the OBM sections were then incubated overnight at 37 degrees Celsius in 1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) with 1% sodium azide dissolved in Dulbecco’s PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg/ml collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/ml DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: Retinas from 2 injected mice for each of the different Cy3-labelled siRNAs were pooled and dissociated into single cells using papain for dissociation according to the manufacture’s instructions (Worthington; Cat# 9035-81-1). Each conjugate was done in triplicates with a total of 6 mice per configuration ...
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bioRxiv - Paleontology 2023Quote: ... The matrix sections were then incubated overnight at 37 degrees Celsius in a solution of ∼1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) dissolved in Dulbecco’s PBS (pH 7.2 ...
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bioRxiv - Biophysics 2024Quote: Hippocampi were isolated from postnatal day 1 (P1) Wistar-ST rats (by Sankyo Labo Service Co., Japan) and treated with papain dissociation system (Worthington Biochemical Co., NJ) according to the company protocol ...
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bioRxiv - Physiology 2020Quote: ... oocytes contained in 1-3 mL of ovary lobes extracted via survival surgery were isolated by enzymatic digestion with collagenase type II (Worthington Biochemical Corp., NJ, USA) under mechanical agitation ...
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bioRxiv - Molecular Biology 2022Quote: Calvarial osteoblasts were obtained from 3 to 5-day-old C57BL/6 mice by sequential enzyme digestion [1 mg/ml collagenase type II (Worthington Biochemical, Lakewood, NJ, USA) in Hanks’ balanced salt solution (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Cortices were isolated from postnatal day 0 (P0) CD-1 or cGKII KO mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp. Lakewood, NJ). Mouse cortical neurons were plated on following poly lysine-coated dishes for each experiment - 24 wells (200,000 cells ...
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bioRxiv - Microbiology 2022Quote: Enzymatic digestion of lung tissue was performed by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and the lateral SVZs were dissected in cold DMEM-F12-glutamax 1/50 Glc 45% and subsequently incubated in digestion medium (papain [Worthington], DNase [Worthington], L-cysteine [Sigma-Aldrich]) for 30 min at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Lungs were digested by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Virus-infected cells were grown in MEM-BSA-TPCK media[20] that was prepared similar to the MEM media described above but supplemented with 1 µg/mL Tosyl phenylalanyl chloromethyl ketone (TPCK) trypsin (Worthington Chemical Corporation, Lakewood, NJ) and with Bovine Albumin Fraction V (7.5% solution ...
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bioRxiv - Neuroscience 2023Quote: Both retinas of three Csf1rGFP mice were dissected at different timepoints (Day 1, 3, and 7) and incubated with papain (Worthington Biochemical, Freehold, NJ, USA) for 15 min as previously described (26) ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM Na-pyruvate) and dissociated them by adding an equal volume of 2 x Papain stock solution (Worthington Biochemical Corporation, Lakewood, USA) at 37°C for 20 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 24 h post-transfection the media was supplemented with 1 μg/mL of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals, Lakewood, NJ, USA). The rgH9N1 viruses were propagated on MDCK cells and harvested 72 hours post-inoculations ...
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bioRxiv - Molecular Biology 2024Quote: ... The fatty and adventitial layers were removed through an 8–10- minute digestion with an enzyme cocktail consisting of 1 mg/ml collagenase type II (Worthington Biochemical Corp, catalog #LS004176), 1 mg/ml soybean trypsin inhibitor (Worthington Biochemical Corp ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated at 37 °C for 1 h followed by the addition of 1.5 mg/mL trypsin inhibitor (Worthington Biochemical Corp, Lakewood, NJ USA) to inactivate trypsin ...
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bioRxiv - Cell Biology 2024Quote: ... the digestion was stopped by a solution of trypsin inhibitors (Leibovitz’s L15 medium, 10% 1 mg/ml trypsin inhibitor [Worthington], and 2% 10 mg/ml DNAse) and cells were washed with L15 medium ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...