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Citations for Worthington Biochemical :
251 - 300 of 694 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Spleens were cut into small pieces and enzymatically digested in 1mL RPMI containing 200 U/mL Collagenase IV (Worthington) and 0.2 mg/mL DNaseI (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... and digested for 30 min using 2mL of HEPES-buffered HBSS containing 20 U/ml papain (Worthington Biochem., USA), 1mM EDTA (PanReac AppliChem ...
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bioRxiv - Neuroscience 2024Quote: ... One ml of the enzyme digestion buffer 2 (HEPES-ACSF) containing collagenase type 2 (2 mg/ml; Worthington, LS004174), dispase II (2 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The organoids were manually dissected into small pieces and transferred into a new 60 mm dish containing 2.5 mL of Enzymatic dissociation solution [composed of 27.3 U/mL papain solution (Worthington; LS003126) supplemented with 200 U/mL of DNase I solution (Sigma ...
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bioRxiv - Immunology 2024Quote: ... and skin was cut into small pieces and mixed with digestion buffer containing 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
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bioRxiv - Immunology 2024Quote: ... and the supernatant was removed and replaced with 1.5 mL Ca2+/Mg2+-free HBSS containing 12 collagenase type II (Worthington) and incubated at 37C for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... PBS was supplemented with DNase solution (containing a final concentration of 200U/ml DNase [Worthington], 2.5mM MgCl2, 500mM CaCl2). 2D adherent cell lines were washed 2X in cold PBS ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... Proteins were released from the DNA by incubation with 5 U of DNA I (Worthington) in 10 mL of 25 mM HEPES-KOH (pH 7.6) ...
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bioRxiv - Immunology 2021Quote: ... Lungs were separated into the left and right lobes prior to dissociation in a Miltenyi Biotec C tube containing 2mL [2mg/mL] collagenase II (Worthington) in RPMI with 8% FBS (RPMI serum ...
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bioRxiv - Cancer Biology 2020Quote: Spleens were collected into RPMI 1640 containing 2% fetal calf serum (FCS) and dissociated with a scalpel and type 3 collagenase (Worthington) and DNAse I ...
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bioRxiv - Cell Biology 2022Quote: ... The tissue pieces were dissociated in phosphate-buffered saline (PBS) containing 0.25% [w/v] trypsin (Worthington Biochemical Corp., Lakewood, NJ), 0.1% [w/v] DNase-I (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly isolated tumorous tissue was finely chopped and incubated for 45 minutes on a rotor at 37°C in RPMI medium 1640 containing 30 lU/ml collagenase IV (Worthington), 12,5 μg/ml DNAse (Roche) ...
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bioRxiv - Physiology 2022Quote: Digestion of the left kidney was performed by a 20 minutes-incubation in HBSS containing type II collagenase at 0.5 mg/ml (Worthington, USA), followed by a 4 minutes-incubation in trypsin 0.04% at 37°C after removal of the capsule ...
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bioRxiv - Immunology 2020Quote: ... Approximately 1cm2 of skin was digested overnight in 5%CO2 at 37°C with 3ml of digestion mix containing 0.8mg/ml Collagenase Type 4 (Worthington; #LS004186) and 0.02mg/ml DNase (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tissue was then titurated in a digestion media containing 10 U/ml elastase (#LS002280, Worthington Biochemical, Lakewood, NJ, USA) and incubated for 30 minutes at 37°C ...
Product cited:
Two times crystallized. Supplied as an aqueous suspension. This preparation must be diluted to...Cat# LS002280, 1 gm, $1065.00 Ask -
bioRxiv - Genomics 2023Quote: ... Cells were then filtered through a 70µm cell strainer into an inactivation solution containing albumin-ovomucoid inhibitor (Worthington, Cat#LK003182), DNAseI (1mg/mL ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and Plp1-TAT-DTR mice (for P42_new IHCs) were dissected out and first digested in a choline chloride solution containing 20 U/mL papain (Cat#: LK003178, Worthington) and 100 U/mL DNase I (Cat# ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Immunology 2022Quote: ... Aortae were dissected and digested in a solution containing of 1200 U/ml collagenase II (Worthington Biochemicals, Lakewood, NJ, USA), 60 U/ml DNase (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: Cerebral cortices from P4-6 CD1 mouse pups were dissected in ice-cold HBSS without Mg2+ and Ca2+ and triturated with 2 ml of digestion buffer containing 20 U/ml papain (Worthington) and 100 U/ml DNase I (Worthington ...
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bioRxiv - Neuroscience 2023Quote: ... The L2-5 ganglia were dissected out and placed in cell culture media before being transferred to media containing neutral protease 3.125 mg/mL (Worthington, LS02104) and collagenase type 1 5 mg/mL (Worthington ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Immunology 2023Quote: ... Aorta samples were cut into small pieces and then transferred into an enzyme cocktail containing 450 U/mL collagenase type I (Worthington), 250 U/mL collagenase type XI (MilliporeSigma) ...
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bioRxiv - Microbiology 2024Quote: ... lungs were harvested and single-cell suspension was obtained by digesting the tissue in RPMI 1640 containing 10 % FBS and 2 mg/mL of type 2 collagenase (Worthington) and 30 µL/mL of 10 mg/mL DNase (Worthington ...
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bioRxiv - Cancer Biology 2024Quote: ... The material was then transferred to a 5 mL microcentrifuge tube containing 2.5 mL Papain supplemented with 125 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Systems Biology 2024Quote: ... WT and Elavl2 knockout EBs were collected into Eppendorf tubes where the medium was replaced with papain solution containing 20 U/mL papain and 67 U/mL DNase (Worthington). Tubes were incubated for 45-60 minutes at 37° C under constant agitation ...
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bioRxiv - Neuroscience 2024Quote: ... The neocortices were isolated from pups on day 0-1 in cold Earle’s Balanced Salt Solution (EBSS) and incubated for 15 min at 37°C in EBSS containing 10 U/ml papain (Worthington), followed by gentle dissociation in PBS containing 1.5 mg/ml BSA ...
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Sex-specific perturbations of neuronal development caused by mutations in the autism risk gene DDX3XbioRxiv - Neuroscience 2024Quote: ... Cortices from single embryos were isolated in ice-cold Hank’s balanced salt solution (HBSS) and then digested with pre-warmed papain containing DNase (Worthington, #LK003150) at 37°C and 5% CO2 for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... minced pieces (1-2 mm3) of isolated cerebral cortices were enzymatically digested in Hank’s buffered saline solution containing papain (20 U/ml, Worthington Biochemical), L-cysteine (1 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary tumor and metastasized lung tissues were digested in 5 mL of enzyme buffer containing 0.2 mg/mL collagenase type-II (Worthington, LS004174), 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were minced and digested using a freshly prepared enzymatic mix containing Collagenase type 2 (2.5 mg/ml; Worthington, LS004174), DNase I (125 U/ml ...
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bioRxiv - Neuroscience 2020Quote: ... transfer either only the cerebellum or the cerebellum including pones to a 15 mL tube containing 20 U/mL papain (Worthington, #LK003178) solved in preparation solution and warmed up to 36 °C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Cancer Biology 2021Quote: ... Samples were washed twice in PBS and added to 50 ml tube containing an enzymatic digestion buffer: collagenase type I (45 units/mL, Worthington Biochemical), collagenase type II (15 units/mL ...
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bioRxiv - Neuroscience 2021Quote: ... SNc regions from the slices were dissected out with a scalpel blade and digested with oxygenated high glucose HEPES-buffered saline containing papain (5–8 U/ml, Worthington, USA) for 20–30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Cortices were then digested for 30 minutes at 37°C using a digestion medium containing papain (25 U/ml, Worthington Biochemical), DNAse I (2000 U/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Cortices were then digested for 30 minutes at 37°C using a digestion medium containing papain (25 U/ml, Worthington Biochemical), DNAse I (2000 U/ml ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/mL of papain (Worthington Biochem.), 1 mM EDTA (PanReac AppliCham ...
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bioRxiv - Physiology 2022Quote: ... after intracardiac perfusion of Phosphate Buffer Saline followed by a solution of HBSS containing type II collagenase at 2 mg/ml (Worthington, USA). Specific diets described below were introduced the day of the first dimerizer injection and maintained until sacrifice.
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bioRxiv - Pathology 2020Quote: ... and submerged into a solution of phosphate-buffered saline containing 0.5 mM of calcium chloride (pH 7.4) and 250 units/mL type III collagenase (Worthington, Lakewood, NJ, USA) at 37°C for 1.5 h or for 3h.[16] The collagenase used has substrate specificity to collagens with lower proteolytic activity than other collagenases ...
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant aspirated before the tissue was incubated in a collagenase solution containing 2 mg/mL collagenase type II (CLS2; Worthington Biochemical) in 5 mL 1X HBSS in a 37°C rocking water bath for 10 minutes ...
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bioRxiv - Physiology 2022Quote: ... The hearts were removed and perfused at 37 °C in Langendorff method with nominally Ca2+-free Tyrode’s solution containing 0.5 mg/ml collagenase (Type II; Worthington, Lakewood, NJ, USA) and 0.1 mg/ml protease (type XIV ...
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bioRxiv - Developmental Biology 2023Quote: ... 3-4 pooled organoids at 3-4 months of age or three single organoids at 2-month age from both haploinsufficient and corrected lines were transferred to a 60 mm dish containing a papain and DNase solution from the Papain Dissociation Kit (Worthington, #LK003150). Organoids were minced into small bits with razors and incubated for 30 min at 37°C on an orbital shaker ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... whole hearts from 28 wk-old ♂ WT and R14Δ/+ mice were cannulated and perfused with modified Krebs buffer containing 2 mg/ml collagenase (Worthington) for 10 min ...
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bioRxiv - Bioengineering 2024Quote: ... The dissected corneas were then incubated overnight at 37℃ in digestion medium containing 0.5 mg/ml hyaluronidase (Worthington Biochemicals, Lakewood, NJ), 2 mg/ml collagenase (Gibco-ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... organoids (single organoids cultured in a 96-well plate or up to 8 organoids cultured in a 6-well plate) were incubated with an enzyme solution containing 10U/ml papain (Worthington, LS003127), 1x EBSS (Sigma-Aldrich ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... tumor samples were transferred into a 5mL Eppendorf tube containing 4.5mL cold enzymatic dissociation mix (100µg/mL Collagenase Type 4 (Worthington Biochemical Corporation #LS004186) and 10 µg/mL DNAse I (StemCell Technologies #07900 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004186, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... was dissected on ice and minced using two razor blades in a few drops of pre-warmed (37°C) Hibernate A medium containing 20 units/mL papain (Worthington LK003178). Minced tissue was incubated in 3.125 mL pre- warmed papain solution for 10 minutes at 37°C ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... PBS was supplemented with DNase solution (containing a final concentration of 200U/ml DNase [Worthington], 2.5 mM MgCl2, 500 mM CaCl2). After the third wash ...
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bioRxiv - Neuroscience 2024Quote: ... The DRGs were then transferred to freshly prepared pre-warmed dissociation medium containing 15U/mL Papain suspension (Worthington Biochemical; Catalog#: LS003126), 0.3mg/mL L-cysteine (Sigma ...
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bioRxiv - Physiology 2024Quote: ... Lung tissues were minced and incubated in 15 mL of conditional medium containing 0.2 mg/mL type I collagenase (Worthington Biochemical Corporation) for 30 min at 37 ℃ with agitation ...
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bioRxiv - Bioengineering 2024Quote: Cardiomyocytes were dissociated by Langendorff heart perfusion of a 37°C digestion perfusion solution containing 300 U/mL collagenase (Worthington, LS004176). Collagenase digested tissue was minced and dissociated cardiomyocytes filtered through a 100 µm mesh filter (Falcon ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 µM ROCK inhibitor Y-27632: MedChem Express HY-10583) containing Collagenase III (300 U/mL: Worthington Biochemical Corporation LS004182) and DNase I (200 U/mL ...