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Citations for Worthington Biochemical :
451 - 500 of 519 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... these tissues were finely minced and then digested at 37℃ in digestion buffer: RPMI 1640 (UCSF Media Production) with 1 mg/mL collagenase IV (Worthington Biochemical) and 0.1 mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Biopsies were mechanically minced with sterile scissors into fragments of less than 1 mm3 and enzymatically digested with 20 mg/mL papain (Worthington #20132), 0.7mg/mL hyaluronidase (Sigma #V900833 ...
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bioRxiv - Immunology 2024Quote: ... The digested samples were washed with PBS and were further digested with 0.05% trypsin-EDTA for 5 minutes and 1 unit/ml neutral protease (dispase) (Worthington Biochemical, LS02109) plus 100 ug/ml Dnase (Roche ...
Product cited:
Animal Free, Partially purified. A lyophilized powder.Cat# LS02109, 1 gm, $171.00 Ask -
bioRxiv - Genomics 2023Quote: ... Each sample was centrifuged at 1200 rpm x5 mins at 4°C and resuspended in 10 mL warmed digestive media (HBSS + 1% HEPES) with 1,000 U/mL collagenase type IV (Worthington, Cat: LS004188), and rotated for 30 min at 37 °C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Cortices were isolated from P0 CD-1 mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp., LK003176). Mouse cortical neurons were plated on following poly lysine-coated glass bottom dishes (500,000 cells) ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003176, 1 vi, $29.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Homogenate was digested for 15 minutes in enzymatic solution (0.2 µm filter sterilised 4.5 U/mL papain [Worthington Biochemicals], 1× DNAse [Worthington Biochemicals], in PBS) at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... WAT depots from Scd1fl/fl mice were minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Biophysics 2022Quote: ... CaCl2 was added to a final concentration of 40 mM (21 µL of 1 M) followed by the addition of 75 U (3 µL of 25 U/µL) of micrococcal nuclease (MNase, Worthington Biochemical) and incubated at 37◦C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (Worthington Biochemical) at 37°C for 16 hours ...
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bioRxiv - Cancer Biology 2023Quote: The largest tumors from female C3-TAg and PyMT mice were minced and dissociated for up to 120 min at 37°C in a mixture of 1 mg/ml Collagenase (Worthington, LS004188), 20 U/ml DNase (Invitrogen ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2023Quote: Two organoids per experimental condition were washed with Ca2+/Mg2+-free DPBS and cut into 1 mm2 pieces and enzymatically digested with papain digestion kit (Worthington, LK003163) with the addition of DNase for 1 hr at 37°C ...
Product cited:
Complete kit as described for product Code: PDS, but without the Earle's Balanced Salt Solution (EBSS).Cat# LK003163, 3 bx, $715.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... hindlimb muscles from mice and hamstring muscles from humans were digested with collagenase II (LS004177, Worthington, 1000 units per 1 ml) for 90 min at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... 1% MEM vitamin solution and freshly added 1.0 μg/ml tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corporation, Lakewood, NJ) incubated at 37 °C ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... and hearts were perfused retrogradely for 15-18 min at 37°C with HEPES-buffer containing 100 mg/mL collagenase type 2 (Cat#9001-12-1, Worthington, USA) and digested ...
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bioRxiv - Neuroscience 2024Quote: ... Terminal differentiation of oligodendrocytes was achieved in oligodendrocyte differentiation media by mitogen withdrawal for 7 days (except for T3 and IGF-1) following single-cell dissociation using Papain (20 units/ml) dissociation kit (Worthington Biochemical) and plating on Matrigel (SLS ...
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bioRxiv - Developmental Biology 2024Quote: ... minced vessels were incubated for 60 minutes at 37°C with agitation in an enzymatic cocktail consisting of 1 mg/ml collagenase I (Worthington, LS004196), 1 mg/ml Dispase II (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... The cardiac ventricles were cut into pieces of approximately 1–2 mm3 and dissociated by trituration in 0.05% collagenase type II (Worthington, Lakewood, NJ, USA) 8-10 times for 5 min each ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue from 2-3 hypothalamiwas pooled into 1-1.5 mL of HABG containing papain (20 u/mL, Worthington Biochemical, Lakewood, NJ, US). Tissue was broken into smaller pieces by pipetting 2-3 times with a P1000 pipet followed by a fire-polished glass pipette with a wide opening ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... The collected nerve segments were cut into small pieces with microscissors and incubated in 1 ml collagenase (4mg/ml Worthington Biochemical, LS004176) and dispase (2mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Genomics 2021Quote: ... Lung tumor sample were cut into small pieces with a razor blade and enzymatically digested (100 U/mL Collagenase IV, Sigma-Aldrich, C5138-1G; 50 µg/mL DNase 1, Worthington, LS002138) for 35 minutes being rotated at 37°C in HEPES buffered RPMI 1640 containing 0.5% FBS ...
Product cited:
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bioRxiv - Neuroscience 2020Quote: ... The collected nerve segments were cut into small pieces with microscissers and incubated in 1 ml PBS supplemented with collagenase (4mg/ml Worthington Biochemical, LS004176), dispase (2mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Systems Biology 2022Quote: ... testes were placed in a 15 ml conical tube containing 2 ml of prewarmed (35 °C) collagenase solution (1 mg/ml type I collagenase (Worthington, cat. # LS004196) and 60 U/ml Turbo Nuclease (Nacalai ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2020Quote: ... the heart was perfused with the same solutions with 50 μM Ca2+ and collagenase type II (1 mg/mL, CLS-2, Worthington, NJ, USA) for 15 minutes ...
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bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
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bioRxiv - Immunology 2021Quote: ... tissue was finely minced and placed into digestion media (RPMI basal media, 300 U/mL collagenase I [Worthington Biochemical Corporation], 1% BCS), shaken for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: Lungs from 8-10 week old mice were dissected and mechanically disrupted using scissors before incubation in RPMI containing 1 μg/ml type II collagenase (Worthington Biochemical Corporation) and 40 U/ml DNase I (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and cut into rings (about 1 mm in length) that were subsequently incubated in 2 mg/ml collagenase type II (Worthington Biochemical Corporation) for 2 hr ...
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bioRxiv - Cell Biology 2023Quote: Tissue samples were dissected into small pieces and digested with 1-2 mg/ml (275 U/ml) collagenase type II (Worthington, Columbus, USA) in 30 ml Dulbecco’s minimal essential medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: The primary neuronal cells were isolated from the brain tissues of 1∼2-day-old SD pups using the papain dissociation system (Worthington Biochemical Corporation) as instructed by the manufacturer ...
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bioRxiv - Biophysics 2023Quote: ... Microsomes (1–2 mg/ml proteins) were digested at progressively increasing enzyme concentrations of TPCK-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ) or chymotrypsin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... The whole lung was minced and enzymatically digested in RPMI 1640 medium containing 1 mg/ml collagenase IV (Worthington Biochemical, Lakewood, NJ) at 37◦C for 30 min ...
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bioRxiv - Neuroscience 2024Quote: Neuronal cells were extracted from the brains of 1-day-pold SD pups using a papain dissociation system (Worthington Biochemical Corporation, Worthington, NJ, USA) following the manufacture’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected frontal cortex (cortex rostral to the optic chiasm) was minced and immersed in an enzyme incubation solution (1 mL) containing 200 U papain (Worthington Biochemical, #LS003126), 0.5 mM EDTA (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... The DRG bulb was then cut into 3mm sections and placed in 5mL of pre-warmed digestion enzyme containing 1 mg/mL of Stemzyme I (Worthington Biochemical, LS004106), 0.1 mg/mL of DNAse I (Worthington Biochemical ...
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bioRxiv - Cell Biology 2024Quote: ... Two to three pieces of 0.5g testis tissue was placed in a 50mL Falcon tube with 10mL prewarmed collagenase solution at 32 °C (1 mg/mL collagenase I (Worthington Biochemicals #LS004196), 1 mM EDTA ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred to a 15ml tube with 8ml collagenase solution (∼4,000 units in αMEM with 1% anti-mycotic; Type IV, Worthington Biochemical Corp, Lakewood, NJ). The solution containing the tissue fragments was then continuously agitated for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Homogenised tissue was added to a solution (total volume of 7 ml) composed by of 1 mg/ml collagenase IV (Worthington Biochemical Corp, USA), 0.02 mg/ml DNase 1 (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
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bioRxiv - Microbiology 2022Quote: ... of 0.01 and maintained in MEM containing 0.3% bovine serum albumin (MEM/BSA) and 1 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-trypsin (Worthington, Lakewood, NJ, USA). Supernatants were collected every 12 h and viral titers were measured using a plaque assay.
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bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue fragments were incubated in 1.35 mL PBS plus 150 uL 20 mg/mL Collagenase Type I (Worthington CLS-1 dissolved in PBS) and 15 uL 20 mg/ml DNase I (Worthington D dissolved in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Brain tissues were manually minced using razor blades and enzymatically dissociated at 37°C for 30 min with 1 mg/mL collagenase type IV (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/mL DNase 1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Paleontology 2023Quote: ... the OBM sections were then incubated overnight at 37 degrees Celsius in 1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) with 1% sodium azide dissolved in Dulbecco’s PBS ...
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bioRxiv - Neuroscience 2023Quote: Retinas from 2 injected mice for each of the different Cy3-labelled siRNAs were pooled and dissociated into single cells using papain for dissociation according to the manufacture’s instructions (Worthington; Cat# 9035-81-1). Each conjugate was done in triplicates with a total of 6 mice per configuration ...
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bioRxiv - Paleontology 2023Quote: ... The matrix sections were then incubated overnight at 37 degrees Celsius in a solution of ∼1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) dissolved in Dulbecco’s PBS (pH 7.2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The obtained liver tissues were homogenized in 8 mL of RPMI1640 media containing 1.8 mg/ml Collagenase IV (WOR-CLS4-1, Worthington Biochemical Corporation, NJ, USA) and 112.3 µg/ml DNase I (11284932001 ...
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bioRxiv - Cancer Biology 2023Quote: ... spun down by centrifugation at 500 x g for 5 minutes and resuspended in 10 ml of digestion medium (MEM containing 112 U/mL type I collagenase; Worthington, cat. #CLS-1), 1.2 U/mL dispase (Gibco ...
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bioRxiv - Biophysics 2024Quote: Hippocampi were isolated from postnatal day 1 (P1) Wistar-ST rats (by Sankyo Labo Service Co., Japan) and treated with papain dissociation system (Worthington Biochemical Co., NJ) according to the company protocol ...