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Citations for Qiagen :
2601 - 2650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... we extracted genomic DNA (gDNA) using the MagAttract High Molecular Weight kit from Qiagen (Valencia, California). Toe pads extraction of historical study skins was carried out in a dedicated historical DNA extraction laboratory at the Academy of Natural Sciences of Drexel University to reduce contamination risk ...
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bioRxiv - Genomics 2024Quote: ... DNA isolation was then performed using the QiaQuick spin columns and extraction kit from Qiagen (Valencia, CA).
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bioRxiv - Biochemistry 2024Quote: ... and the clarified lysate was applied to a column of Ni-NTA resin (Qiagen and ThermoFisher) preequilibrated in wash buffer 1 (50 mM HEPES ...
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bioRxiv - Genomics 2024Quote: ... DNA extraction was carried out with the sample powder using a Qiagen MagAttract HMW kit (Qiagen Cat. No. 67563) following the manufacturer’s protocol with some modifications ...
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bioRxiv - Immunology 2024Quote: ... 2 × 104 cells were sorted in triplicate per sample and stored at −80°C in RLT buffer (Qiagen). RNA was isolated using the Qiagen RNeasy Micro Kit ...
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bioRxiv - Immunology 2024Quote: ... and purified with RNeasy Mini Kit (74106, Qiagen) according to the manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... Following purification with the PCR Purification Kit (QIAGEN), fragment sizes were confirmed using the 2200 TapeStation and High Sensitivity D1000 ScreenTapes (Agilent) ...
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bioRxiv - Immunology 2024Quote: TET2 knockout efficiency was confirmed by isolating genomic DNA from CAR T-cells at Day 7 using the dNeasy Blood & Tissue Kit (Qiagen). PCR of genomic DNA was performed with TET2 Forward Primer 5’-TCCCTGAGTCCCAGTCCATC-3’ and Reverse Primer 5’-TCAGGAATGGCCAGGTTCTG-3’ using MyTaq Red 2X Mix (Meridian Bioscience) ...
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bioRxiv - Genomics 2024Quote: ... immersed in RNAlater Stabilization Solution (Qiagen Inc., USA) and stored at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... or from the scleras of the control corneoscleral discs (QIAgen Blood and Tissue kit) in accordance with the manufacturer’s guidelines.
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bioRxiv - Evolutionary Biology 2024Quote: ... We extracted genomic DNA (gDNA) from individual lice (i.e., not pooled individuals) using a modified protocol with Qiagen QIAamp DNA Micro Kits (Qiagen, Hilden, Germany). Specifically ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were pooled and cleaned up by Qiagen MinElute PCR Purification kit following manufacturer’s instruction (Qiagen cat ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was purified using RNeasy mini kits (Qiagen). cDNA was generated using Double Primed EcoDry kits (Takara) ...
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bioRxiv - Developmental Biology 2024Quote: ... 1.5 µg of gDNA was eluted in 15 µl of elution buffer from the EpiTect Fast DNA Bisulfite kit (Qiagen, 59824). A 20 µl PCR reaction was composed of 0.5 µl of bisulfite-converted gDNA input ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA (gDNA) was extracted from whole embryos using the Gentra Puregene Tissue kit (Qiagen, 15063), according to the manufacturers’ specifications with one modification ...
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bioRxiv - Cell Biology 2024Quote: ... which was performed using the manufacturer’s protocol (#12123, Qiagen Plasmid Minikit). Isolated plasmid was then sent for Sanger Sequencing to confirm the insertion of point mutations at the desired location and then used for further transfection experiments.
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bioRxiv - Developmental Biology 2024Quote: ... and purified using the MinElute Cleanup kit (Qiagen). pUAST vector was double digested using EcoRI-HF and HpaI (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... PA and OA treated (24h and 1week) samples using the RNeasy Micro Kit (Qiagen). Library preparation and sequencing was performed at the Wellcome Trust Sanger Institute (Hinxton ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the MinElute PCR purification kit (Qiagen). Fragments were digested using EcoRI-HF (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were sorted directly into the β-mercaptoethanol supplemented RTL Plus buffer of the RNeasy Plus Micro Kit (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: ... the expression vector reported previously (Macfarlan et al., 2012) was isolated from a bacterial extract using the EndoFree Plasmid Maxi Kit (Qiagen #12362), digested with SacII for linearization ...
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bioRxiv - Developmental Biology 2024Quote: ... Final PCR products of 250–500 bp were excised from a 2% agarose gel and purified using a gel purification kit (Qiagen, Catalog # 28604) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted using the Qiagen RNeasy kit (Qiagen; 74106) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR analysis was performed in triplicate using SYBR green master mix (Qiagen, 204143) on a QuantStudio6 Real-Time PCR System ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from flash frozen pancreas tissues using the RNeasy Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were subsequently passed through a QIAshredder column (Qiagen, 79654) to eliminate debris before proceeding with nucleic acid purification ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated with the RNeasy mini kit (Qiagen, Venlo, The Netherlands) and mRNA expression was determined with the HT-12 v4 chip (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA extractions from each sample were performed using RNeasy Fibrous Tissue Mini Kit (Qiagen). Extracted total RNA quality and concentration were assessed on a NanoDrop Spectrophomoter (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Specific siRNA oligonucleotides for β-catenin and control siRNA were obtained from Qiagen, Caveolin-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were digested with proteinase K overnight and DNA was extracted the next day by QIAamp DNA Mini Kit (ref 51306) (Qiagen, USA) with washes performed twice ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP DNA was purified using Qiagen MinElute Reaction Cleanup kit (Qiagen) following factory protocols.
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bioRxiv - Cancer Biology 2024Quote: ... Tagmented DNA was purified using the MinElute Reaction Cleanup Kit (Qiagen).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were collected at around 80% confluence and snap-freeze on dry ice before batched processed using rNeasy Mini Kit (QIAGEN #74106) as per supplier instructions and eluted in 50 µl nuclease free water (Invitrogen #10977049) ...
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bioRxiv - Cell Biology 2024Quote: ... all samples harvested in different batches were processed altogether using RNAeasy Kit (Qiagen). For RNA-seq ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were resuspended in RNA lysis buffer (RNeasy Kit, Qiagen) and store in -80°C condition ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from was extracted using the RNeasy Micro Kit (Qiagen, Madrid, SP). Complementary DNA using 0.1 to 1 µg RNA was synthesized using the Superscript III Reverse Transcriptase (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from human primary immune cells using the RNeasy Plus Micro Kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Neuroscience 2024Quote: ... or miRCURY LNA RT Kit (Qiagen Sciences) for analysis of mRNA or miRNA respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP enrichments were analysed by qPCR using the QuantiNova SYBR Green PCR kit (Qiagen; 208054).
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted using RNeasy plus mini kit (Qiagen, 74136) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... we synthesised cDNA using 2 µg of total RNA and cDNA was amplified with a PerfeCTa qPCR fluorescent SYBR Green SuperMix (Quantabio) using real-time quantitative PCR (Rotor-Gene RG 6000— QIAGEN). The primer sequences used for the gene expression are shown in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: gDNA was isolated from mature tobacco leaves using the DNeasy® plant Pro Kit (Qiagen). The TRIzolTM reagent (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 25 mg of whole kidney tissue by manual homogenization using a 25-gauge needle in RLT buffer as described by the manufacturer (Qiagen RNeasy Mini Kit). The purity of RNA was evaluated using a spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and purified with MinElute kit (Qiagen). RNA quality was determined using Experion RNA StdSens analysis kit (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... amplification system using RT² SYBRβ Green qPCR Mastermixes (Qiagen) and primer sets specific for each gene (Table S1) ...
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bioRxiv - Cell Biology 2024Quote: mRNA was extracted using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands) and cDNA prepared using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... vectors were amplified by endotoxin-free Giga prep (Qiagen 12391 or Zymo Research D4204). NEB Stable chemically competent E ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA generated after performing reverse transcription of RNA product was subjected to quantitative PCR (qPCR) analysis using gene-specific predesigned primers (RT2 qPCR Primer Assay, Qiagen), listed in Supplementary Table S4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The following siRNA were transfected at 100 μM using HiPerFect (Qiagen): ATAD5 (HSS129125) ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted using the miRNeasy Micro kit (Qiagen) according to manufacturer’s protocol ...