Labshake search
Citations for Qiagen :
1951 - 2000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... followed by purification using QIAGEN-tip20 (QIAGEN, Venlo, Netherlands). To construct the circular double-stranded DNA for pNGS2-BC12-libraries containing either an 8-oxo-G or lesion-free ...
-
bioRxiv - Developmental Biology 2024Quote: ... and RNA was isolated using the RNAeasy kit (Qiagen). cDNA was produced with the iScript cDNA kit (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2024Quote: ... and RNA purified from 5-dpf embryos with the miRNeasy Mini Kit (Qiagen, catalog #: 217004). Using this cDNA library ...
-
bioRxiv - Developmental Biology 2024Quote: ... purified with an RNeasy mini kit (Qiagen, catalog #: 74104), and dissolved in 50% formamide for storage at -80°C.
-
bioRxiv - Developmental Biology 2024Quote: ... The pellet was resuspended in 100μl TE buffer (QIAGEN) and 100μl of Phenol-chloroform was added ...
-
bioRxiv - Cell Biology 2024Quote: ... RNA was extracted with the RNeasy RNA extraction kit (Qiagen) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: DNA was isolated from individual Drosophila adults using a modified DNeasy Blood and Tissue DNA extraction kit (Qiagen), as previously described (Nelson et al ...
-
bioRxiv - Cell Biology 2024Quote: ... The digested plasmid was then purified using the QIAEXII Gel Extraction Kit (Qiagen, Q20021) and diluted to a final concentration of 100 ng/μL and ready for transformation.
-
bioRxiv - Cell Biology 2024Quote: FlexiTube siRNA Premix siRNA (Qiagen, Germany): SI01513407|S5 ...
-
bioRxiv - Cell Biology 2024Quote: RNA was extracted using the RNEasy Mini Plus kit (Qiagen, Germany). For NHEKs the J2s were removed with a single 5-minute wash with versene prior to lysis ...
-
bioRxiv - Cell Biology 2024Quote: ... DNA extraction was then completed using the DNeasy Tissue and Blood Kit (Qiagen, 69504) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Equal quantities of RNA were transcribed into cDNA using QuantiTect Reverse Transcription kits (Qiagen), and analysis of targets gene expression was performed on a 7500 Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA was from extracted from cultured EnSCs and purified through columns using the RNeasy Plus Universal Mini kit exactly as per manufacturer’s instruction (Qiagen, Manchester, U.K.). Recovered RNA in nuclease-free water was analyzed on a Nanodrop spectrophotometer where concentration and purity (230/280 and 260/280 nm ...
-
bioRxiv - Cell Biology 2024Quote: RNA isolation of primary cells or organoids was performed following manufacturer’s instructions using RNeasy Mini kit (Qiagen). Extracted RNA was DNAse treated ...
-
bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated with the miRNeasy Micro kit (Qiagen) following manufacturer instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... cleaned-up using the RNeasy kit (Qiagen, UK) and cDNA was synthesized with the High Capacity cDNA Transcription kit (Applied Biosystems ...
-
bioRxiv - Cell Biology 2024Quote: ... The Endo-free plasmid maxi kit (#12162, Qiagen, Valencia, CA, USA) was used for plasmid purification.
-
bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted from zebrafish tissue using a Puregene Core Kit (Qiagen, Germantown, MD). The genomic region containing the desired large deletion was amplified using primers external to each cut site ...
-
bioRxiv - Immunology 2024Quote: ... and FO cells from three healthy individuals using the RNeasy plus Micro Kit (Qiagen). RNA-seq libraries were prepared as previously described [47] ...
-
bioRxiv - Immunology 2024Quote: ... 35,57,59,61 RNA purification was performed using a DNase digestion kit (RNase-Free DNase Set, Qiagen 79254), miRNeasy Mini kit for RNA isolation (Qiagen 217004) ...
-
bioRxiv - Immunology 2024Quote: ... miRNeasy Mini kit for RNA isolation (Qiagen 217004), and nuclease-free water (Life Technologies AM9937 ...
-
bioRxiv - Immunology 2024Quote: ... or PMs were washed with PBS and then lysed in a culture plate using QIAzol Lysis Reagent (Qiagen 79306) as was described in our previous studies ...
-
bioRxiv - Immunology 2024Quote: ... the superior lobe was homogenized in RLT Plus buffer (QIAGEN, #1053393) with β-mercaptoethanol following storage at −80°C in RNAlater (ThermoFisher ...
-
bioRxiv - Immunology 2024Quote: ... Recombinant plasmids were extracted by using the EndoFree Plasmid Maxi Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... One μg of RNA was reverse-transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen) containing both oligo-dT and random primers.
-
bioRxiv - Immunology 2024Quote: ... TCRα/β were amplified using OneStep RT-PCR kit (Qiagen) according to the manufacturer recommendation ...
-
bioRxiv - Immunology 2024Quote: ... The viral RNA from the supernatant was purified using QIAamp viral RNA mini preps (Qiagen, #52904). The final elution was 60 µl using the supplied buffer.
-
bioRxiv - Immunology 2024Quote: ... Fecal genomic DNA was extracted with the DNeasy Blood and Tissue Kit (QIAGEN, Germantown, MD) using a previously published protocol that was modified slightly for optimization 55 ...
-
bioRxiv - Immunology 2024Quote: Colon was homogenized in RLT buffer (RNAeasy kit, Qiagen, Valencia, CA) with a tissue homogenizer ...
-
bioRxiv - Immunology 2024Quote: ... RNA was extracted using the RNeasy Mini Kit (Qiagen, 74106) and cDNA was synthesized using high-capacity cDNA reverse transcription kit (Applied Biosystems ...
-
bioRxiv - Immunology 2024Quote: Total RNA from all the groups mentioned above was isolated from approximately 2 × 106 cells with the RNeasy minikit (Qiagen, USA), and cDNA was prepared with the iScript Reverse transcription kit (Bio-Rad ...
-
bioRxiv - Immunology 2024Quote: ... and TissueLyser LT (Qiagen) per manufacturer methods ...
-
bioRxiv - Immunology 2024Quote: ... and the DNA was purified with the EpiTect ChIP OneDay kit (Qiagen) as recommended by the manufacturer ...
-
bioRxiv - Immunology 2024Quote: ... The cells were cross-linked and lysed with the EpiTect ChIP OneDay kit (Qiagen 334471), as recommended by the manufacturer ...
-
bioRxiv - Immunology 2024Quote: ... Pathway analysis was performed with host DEGs using Ingenuity Pathway Analysis (IPA, v84978992) (QIAGEN) using the Canonical pathways ...
-
bioRxiv - Immunology 2024Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cý primer ...
-
bioRxiv - Immunology 2024Quote: ... T157I mutations (23) were produced by Hi5 insect cells and extracted from culture supernatant using Ni-NTA (Qiagen). HLA-DR monomers were biotinylated overnight at 4°C using BirA biotin ligase (Avidity ...
-
bioRxiv - Immunology 2024Quote: RNA was extracted from FACs sorted macrophages using RLT(lysis) buffer and an RNAeasy Kit (Qiagen, Hilden, Germany). Concentration and purity were assessed using a Nanodrop 1000 (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... and ACTB (QIAGEN, Cat. No. Hs_ACTB_1_SG) and GAPDH (IDT ...
-
bioRxiv - Immunology 2024Quote: ANKRD55 interactome proteins were analyzed with the use of Ingenuity Pathway Analysis (IPA) software from QIAGEN (https://digitalinsights.qiagen.com/IPA) ...
-
bioRxiv - Microbiology 2024Quote: ... we followed the QIAamp DNA stool mini kit (Qiagen)-based protocol described by Knudsen et al ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was extracted from an additional individual using DNeasy Blood & Tissue Kit (Qiagen). Long reads were produced by Minion (Oxford Nanopore) ...
-
bioRxiv - Microbiology 2024Quote: ... DNA bands were excised and purified using the QIAquick Gel Extraction Kit (Qiagen, USA) and sent for commercial Sanger sequencing (through Genewiz ...
-
bioRxiv - Immunology 2024Quote: Total RNA was isolated using QiaShredder columns and the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: Genomic DNA was either extracted from cell pellets using the DNeasy Blood & Tissue kit (Qiagen) with addition of 50 µg RNaseA in the pellet resuspension step or cell pellets were prepared by direct lysis using home-made quick extract buffer (1 mM CaCl2 ...
-
bioRxiv - Genomics 2024Quote: RNA was extracted from mouse tissue samples using an AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA). Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen ...
-
bioRxiv - Genomics 2024Quote: ... Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen, Valencia, CA) and kept on ice until use ...
-
bioRxiv - Genomics 2024Quote: ... DNA extraction was performed with the Qiagen MagAttract HMW kit (Qiagen Cat. No. 67563) following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2024Quote: RNA was extracted from 2-5 million flash frozen cells using the RNeasy plus mini kit (Qiagen). Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs) ...
-
bioRxiv - Genomics 2024Quote: ... DNA was extracted and purified with the DNeasy Plant Mini Kit (Qiagen) and 130 ng were used for MethylC-seq library preparation ...