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Citations for Qiagen :
1551 - 1566 of 1566 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... we placed filters in 2 mL tubes with beads and 1 mL TE buffer (Qiagen plasmid isolation kit, Qiagen Germantown, MD, USA) and ran them in the tissue lyser at either ½ speed or max speed for 30 sec or 1 min ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal volume of inoculum from each pool was then combined and used as starter culture for a single maxiprep plasmid isolation of the pooled HDR template library (Qiagen Maxiprep kit).
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bioRxiv - Genetics 2023Quote: ... 1 mL glycerol stocks were made for each library and a 200 mL LB-AMP outgrowth was grown to a concentration of 3 OD600/mL followed by a Qiagen MAXI plasmid prep (Qiagen Cat#12963).
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA (gDNA) was prepared in a plasmid- and PCR product-free work area using a QIAamp DNA Blood Maxi Kit (Qiagen, Cat. #51192) according to manufacturer instructions and previously published protocols27 ...
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bioRxiv - Genetics 2020Quote: ... PCR reactions were cleaned up to remove unamplified circular plasmid template by gel extraction followed by digestion with DpnI and a PCR cleanup reaction (Qiagen PCR Purification kit). Mat a yeast were then transformed with the cloNAT resistance cassette ...
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bioRxiv - Genetics 2021Quote: ... Isolation of the plasmid DNA from bacterial clones was conducted using Zymo or Qiagen mini prep and maxi prep kits (Qiagen #27104, Zymo #D4208T)
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bioRxiv - Genetics 2020Quote: ... we grew one aliquot overnight in 400 mL LB + carbenicillin medium at 30°C and performed three maxi preps and two mega preps (200 mL each) using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965, #12981). For the Upstream library ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were plated at a density of ∼100,000 cells/well in a 24 well plate and transfected the following day with 500 ng of midi-prepped endotoxin-free plasmid DNA (Qiagen, Germantown, MD, USA) using Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with 500 ng of complementation plasmid (described before) + 25 ng of pCoBlast (Thermo Fischer, Cat. No K5150-01) using Effectene Transfection Reagent (QIAgen, Cat. No 301425). 48 h after transfection ...
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bioRxiv - Genetics 2022Quote: ... the PCR fragment and the plasmid fragment without PDS gene were isolated from agarose gel using QIAquick Gel Extraction Kit (Qiagen, catalog number 28706). The digested PCR products and the pBSMVγ plasmid were ligated using T4 ligase (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... 5µg of plasmid pLentiGuide-Puro was digested with BmsBI (Fermentas, Cat No. ER0451) and purified using the QIAquick Gel Extraction Kit (Qiagen, Cat No. 28704). Each pair of oligos was annealed and phosphorylated with T4 PNK (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... The plasmid library pool was isolated from overnight bacteria culture using QIAGEN HiSpeed Plasmid Midi (small pooled library) of maxi (TSS tiling library) kits (QIAGEN, 12643 and 12662). Lentivirus was produced from cloned plasmid pools in 293FT cells as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspend the cells in 10 ml of suspension buffer (10 mM EDTA pH8.0, 150 mM NaCl, 1% glycerol, Lysis blue (1×, from QIAGEN Plasmid Plus Midi Kit), RNase A (0.55 mg/ml) ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was eluted with 66 µL of 2 mM Tris-HCl pH=7.0 to carry out Solution A purification or eluted with 66 µL of 0.1 X EB buffer (From QIAGEN Plasmid Plus Midi Kit) without further Solution A purification.
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bioRxiv - Cancer Biology 2024Quote: ... cultures and verified with DNA digestion and gel electrophoresis before clonal expansion and purification with QIAGEN Plasmid Maxi Kit was performed (12163; Qiagen, Redwood City, CA, USA). Lentivirus particles were produced in HEK293T cells after co-transfection of lentivirus plasmid vectors with packaging plasmids (VSVG and lenti gag/pol ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA of cells co-transfected with a plasmid expressing sgRNA and another expressing SpCas9 was isolated and purified through a commercially available kit (Qiagen, QIAamp DNA Mini Kit Cat# 51304) as per the manufacturer’s protocol ...