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Citations for Qiagen :
1401 - 1450 of 1566 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... was PCR amplified from plasmid (pSH515) containing the native His4 gene promoter and purified by PCR cleanup kit (Qiagen). DNA was eluted from the spin column with 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: 1×106 MIA PaCa-2 or HAP1 cells were seeded per 6 well plate and co-transfected with 0.5 µg pCas-Guide plasmid using Effectene transfection reagent (Qiagen). 24 hrs after transfection ...
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bioRxiv - Genetics 2020Quote: ... The sgRNA plasmid was linearized with DraI and then purified using a MinElute PCR Purification Kit (Qiagen, Duesseldorf, Germany). sgRNA was produced using the MEGA shortscript Kit (Ambion ...
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bioRxiv - Biochemistry 2020Quote: ... Standard molecular biological and microbiological techniques were employed for plasmid DNA extraction (QIAprep Spin Miniprep Kit, QIAgen, Venlo, Netherlands), confirmation of Cys residue position via plasmid-based sequencing (Eurofins Genomics ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK-293T cells were next transfected with pMMP vectors together with retrovirus packaging plasmids using Effectene transfection reagents (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: Cells (200,000 for HeLa and hiPSC-CMs and 500,000 for NRCMs) were seeded on glass cover slips and transfected with sensor plasmids using Effectene (Qiagen) (HeLa ...
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bioRxiv - Immunology 2020Quote: ... Freestyle 293F (ThermoFisherScientific, Cat#R79007) cell was transfected with purified miniprep plasmid DNA (QIAgen minipreps kit, Qiagen, Cat#29107) as described (Lipofectamine™ RNAiMAX Transfection Reagent ...
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bioRxiv - Immunology 2020Quote: ... Freestyle 293F (ThermoFisherScientific, Cat#R79007) cell was transfected with purified miniprep plasmid DNA (QIAgen minipreps kit, Qiagen, Cat#29107) as described (Lipofectamine™ RNAiMAX Transfection Reagent ...
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bioRxiv - Systems Biology 2022Quote: ... the bacteria cells were scraped and centrifuged at 3,000g for 10 min and the plasmids were then extracted (Qiagen). Cloning of individual sgRNA base editor construct was based on similar procedure with reduced reagent input.
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were normalised to cell density and DNA was isolated using the Plasmid Maxi Kit (Qiagen, Cat. No. 12163) according to manufacturer’s directions except that all steps and reagents ...
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-ZMYM2(WT) (pAS4329) or EGFP-ZMYM2(SIMmut) (pAS4330) plasmids using Polyfect Transfection Reagent (Qiagen, Cat. No. ID: 301105) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... and plasmid DNA from 200-500 µl of the cell suspension was purified using QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Colonies were cultured in 2x YT liquid media overnight and plasmids were extracted using QIAprep Spin Miniprep kit (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into stbl3 competent E.Coli and DNA was harvested from 200 mL growth media using an endotoxin free plasmid purification kit (Qiagen).
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bioRxiv - Genetics 2023Quote: ... Bacterial cells were cultured overnight in liquid and the final libraries were extracted using QIAGEN Plasmid Maxi Kit (QIAGEN). The libraries were quantified and subpools combined in 1:4.76 molar ratio to get the final library containing 5,760 gRNAs.
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bioRxiv - Cell Biology 2023Quote: ... Overnight cultures were pelleted at 3,000g for 10 min and plasmid DNA was purified using a Qiagen miniprep kit (27106, Qiagen). Sequences were verified by Sanger sequencing (Eton Bioscience Inc).
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bioRxiv - Systems Biology 2023Quote: ... The supernatant was recovered and plasmid DNA was purified following the standard QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany) protocol and eluted in 50 μl EB buffer.
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bioRxiv - Cancer Biology 2023Quote: ... and 9-10 mL of Buffer BB (1/3 of the lysate volume, From QIAGEN Plasmid Plus Midi Kit) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... Crude eccDNA was then eluted by using 100 µL of 0.1x EB buffer (From QIAGEN Plasmid Plus Midi Kit). For each microgram crude eccDNA we spiked in 1 ng pUC1932 (was a gift from Joachim Messing ...
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bioRxiv - Cell Biology 2023Quote: ... Linearized plasmids were purified using a QIAquick PCR purification kit according to the manufacturer’s instructions (28106, Qiagen, Hilden, Germany). Templates for CLN3 RNA synthesis were in the same manner but using CLN3 specific primers (for ...
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bioRxiv - Biochemistry 2023Quote: ... An endotoxin-free preparation of the cloned vector was made using the EndoFree Plasmid Maxi Kit (Qiagen, Hilden, DE).
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bioRxiv - Microbiology 2024Quote: ... was used to amplify DNA fragment except for PCR verification of plasmids or strains for which Taq polymerase (Qiagen) was used ...
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bioRxiv - Microbiology 2024Quote: ... The viral RNA product was then treated by DNase to remove the carry-over plasmid DNA (RNeasy Kit, Qiagen). cDNA of the virus genome was generated by reverse transcription with random hexamer as the primer (SuperScript III ...
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bioRxiv - Biochemistry 2023Quote: ... Cx43 constructs with a stop codon in the eGFP-NI plasmid were purified with QIAprep Midi Prep Kit (Qiagen) suitable for Lipofectamine2000 (Invitrogen/Thermofisher ...
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bioRxiv - Genomics 2024Quote: ... each 50 mL midiprep culture was spun down for 30 minutes at 4,300 x g and plasmid DNA isolated using a midiprep kit according to the manufacturers instructions (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded in 24-well plates and transfected with the desired plasmids using Effectene Transfection Reagent (#301427, QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... The mutagenesis plasmid pGJJ055 was digested with HindIII-HF and AvrII and purified with the MinElute PCR Purification Kit (QIAGEN). The GRB2 mutagenesis plasmid (pGJJ057 ...
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bioRxiv - Cell Biology 2019Quote: ... insertion of the gRNA was confirmed by gel electrophoresis of PCR products and the plasmid was purified using the QiaQuick Gel Extraction kit (Qiagen) following provider’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cyp6a8-pOT2 and Cyp6d5-pOT2 plasmids were linearized overnight and purified into RNAse-free water using the MinElute Reaction Cleanup Kit (Qiagen). Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7 ...
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bioRxiv - Molecular Biology 2021Quote: ... Five colonies with the correct sized insert were inoculated into liquid LB supplemented with spectinomycin and chloramphenicol and grown overnight at 37°C for plasmid extraction using a QIAprep Spin Miniprep Kit (Qiagen). The plasmid constructs were confirmed by Sanger sequencing ...
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bioRxiv - Genomics 2020Quote: ... and a library representation of >200 colonies per guide was determined prior to CRISPR library pool plasmid purification by Endofree Maxi (Qiagen). Coverage of the 818 guide library for Perturb-CITE-Seq as well as the extended control library was confirmed by PCR amplification of the guide sequence from the final library pool and deep sequencing on an Illumina MiSeq.
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bioRxiv - Genetics 2019Quote: ... After 16 hrs in a 37°C shaker bacteria were harvested and plasmid DNA was isolated using a Megaprep kit (Qiagen). ITR integrity was confirmed by digestion with XmaI as well as with AhdI ...
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bioRxiv - Microbiology 2019Quote: ... Genes were predicted using Prokka (78) and plasmids were annotated and visualized via CLC Sequence Viewer (v8.0.0) (Qiagen, Aarhus, Denmark). For clade-to-clade chromosome comparisons ...
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bioRxiv - Genetics 2020Quote: ... Final CRE-reporter plasmids were isolated using Qiagen miniprep columns and were further purified on a Qiagen PCR purification column (Qiagen), and diluted to 50 ng/ml with nuclease free water ...
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bioRxiv - Bioengineering 2020Quote: Plasmid isolation from yeast was performed according to a user-developed protocol from Michael Jones (protocol PR04, Isolation of plasmid DNA from yeast, QIAGEN) using a QIAprep Spin Miniprep kit (QIAGEN ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting lawns were scraped off the agar plates and the plasmid libraries were extracted using a QIAprep Spin Miniprep Kit (QIAGEN). The plasmid libraries were subsequently used for yeast transformation.
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bioRxiv - Bioengineering 2020Quote: ... The PCR fragments were incubated with DpnI for 2 h at 37°C to degrade the template plasmids before being purified using a PCR purification kit (Qiagen). The purified PCR fragments were digested overnight at 37°C using NheI and XhoI and ligated overnight at 16°C to backbone vector pYZ125 ...
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bioRxiv - Microbiology 2020Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen, Valencia, CA). A minimum of 20 clones from each sample were sequenced ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were subsequently cloned into pGEM®-T Easy Vector plasmids in a JM109 High- Efficiency Competent Cells (Qiagen) strain of Escherichia coli ...
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bioRxiv - Genomics 2020Quote: ... Cells were seeded in 6-well plates at a density of 1×106 cells/mL and transfected with 200 ng of plasmid using the Effectene transfection kit (Qiagen). Cells were harvested following 3 days of incubation.
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bioRxiv - Molecular Biology 2020Quote: ... The integration of plasmids into the endogenous loci was verified by PCR on genomic DNA extracted using the DNeasy Blood & Tissue Kit (Qiagen).
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bioRxiv - Plant Biology 2020Quote: ... Single colonies were used for inoculation of overnight culture and plasmids isolated with the help of the QIAprep Spin Miniprep Kit (Qiagen). The presence of the mutation was verified by Sanger sequencing (LGC genomics) ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293 cells were transfected with plasmids (WT mGlu1, the mGlu1 mutants, or the control vector) using SuperFect transfection reagent (Qiagen) or Viafect (promega ...
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bioRxiv - Cell Biology 2021Quote: Transgenic parasites were generated by electroporation of sorbitol-synchronized ring-stage parasites with 50-100 μg of purified plasmid DNA (Qiagen). To select for the plasmids pArl-PfCentrin1-GFP and p3-NLS-L3-mCherry ...
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bioRxiv - Cancer Biology 2020Quote: ... mito-roGFP and Myc-epitope-tagged murine Serpin E13,22,23 were purified with Plasmid Plus Midi columns (Qiagen, Inc., Germantown, MD) and administered to 6–8-week-old FVB mice via HDTVI (10 μg each ...
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bioRxiv - Bioengineering 2022Quote: DNA templates for generating desired RNA products were created by PCR amplification from plasmids or gBlock gene fragments (IDT) and purified using a PCR purification kit (Qiagen). Plasmids were then generated with these templates containing a T7 promoter followed by 5’ ribozyme sequence ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant protein fragments were produced in Escherichia coli (strain M15) as fusion products with an N-terminal tag of six histidines using plasmid pQE30 (Qiagen) and purified from total bacterial lysates by nickel affinity chromatography ...
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bioRxiv - Microbiology 2022Quote: ... and plasmids were subsequently isolated from individual colonies grown overnight in LB broth by using a mini-prep kit (Qiagen). All plasmids were verified for correct size by digest ...
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bioRxiv - Microbiology 2021Quote: ... Resulting colonies were expanded in LB broth with ampicillin and plasmid was isolated using the QIAprep Spin Miniprep Kit (Qiagen). All constructs were confirmed by sequencing.
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bioRxiv - Neuroscience 2020Quote: ... GluA1 or GluA2Q (pIRES2-mCherry or pIRES2-EGFP) and CNIH2 (pRK5 or pBOS) plasmids were transfected at a 1:2 ratio using Effectene (QIAGEN) into adherent HEK293T cells (ATCC ...