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Citations for Qiagen :
1351 - 1400 of 1566 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Previously cloned CADPS2 plasmids (Accession: DV955943) were grown from bacterial stock and collected via miniprep columns (Qiagen 27104). CADPS2 was verified for sequence identity and orientation using Sanger sequencing services provided by Eton Biosciences or GeneWiz ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were extracted from the cultures using the QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany, catalogue no. 27106) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... plasmids and growth conditions) were digested using NotI restriction enzyme and purified using QiAquick PCR purification kit (Qiagen). One microgram of cpsA-gfp ...
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bioRxiv - Biochemistry 2019Quote: ... DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Bioengineering 2019Quote: ... The selected colony with confirmed insertion sequence was cultivated and DNA extracted (Plasmid Plus Midi Kit, Qiagen, Netherlands) and then excised from the gel (Sigma-Aldrich x-tracta Gel Extraction Tool ...
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bioRxiv - Bioengineering 2019Quote: ... The selected colony with confirmed insertion sequence was cultivated and DNA extracted (Plasmid Plus Midi Kit, Qiagen, Netherlands) and then excised from the gel (Sigma-Aldrich x-tracta Gel Extraction Tool ...
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bioRxiv - Genomics 2019Quote: ... Library vectors with insert (pJDrcEPP_lib_minPLuc2) were isolated from the remainder transformants using Qiagen Plasmid Plus Maxi Kit (Qiagen).
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bioRxiv - Molecular Biology 2021Quote: A plasmid containing the modified Widom 603–42 sequence31 was purified with a QIAquick PCR Purification Kit (Qiagen) and used as the template for the amplification.
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bioRxiv - Biophysics 2019Quote: ... The SpyTag-(Cpa)4-SpyTag and the HaloTag-SpyCatcher constructs were cloned into the expression plasmid pQE80L (Qiagen). Protein expression and purification was done as described elsewhere36 ...
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bioRxiv - Neuroscience 2021Quote: ... Viral DNA and standard plasmid DNA were extracted using the QIAGEN DNeasy Blood and Tissue kit (QIAGEN #69504). qPCR reactions of serial-dilutions of the DNA samples were prepared using TaqMan Universal PCR Master Mix (ThermoFisher #4304437 ...
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bioRxiv - Biochemistry 2021Quote: DNA constructs were amplified in Escherichia coli DH5α and purified using a Hi-Speed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were amplified in Escherichia coli (XL-Blue) extracted by alkaline lysis and purified by column separation (Qiagen). The integrity was confirmed by gel electrophoresis in 0.8% agarose ...
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bioRxiv - Cell Biology 2022Quote: ... PLA was performed on S2 cells that were transfected with the following plasmids using Effectene Transfection Reagent (QIAGEN): control Myc ...
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bioRxiv - Immunology 2022Quote: ... Plasmid extraction was carried out with Qiagen QIApep® spin miniprep kit following manufacturer’s instructions (QIAGEN Ltd, UK). Inserts were confirmed by EcoRI restriction digestion and gel electrophoresis ...
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bioRxiv - Molecular Biology 2022Quote: ... coli grown for 16 h in 250 ml of 2×YT medium using the Plasmid Maxi Kit (QIAGEN) in accordance with the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were prepared by mini or maxi preps using commercially available kits (Qiagen, UK and Promega, UK, respectively) following manufacturers’ instructions ...
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bioRxiv - Biophysics 2023Quote: ... we isolated cosmid-i95 or pBS-T7A1-parS via a midiprep using a Qiafilater plasmid midi kit (Qiagen). We digested the cosmid-i95 for 2 h at 37°C using SpeI-HF restriction enzyme (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Microbiology 2023Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen). Primers ABR-RT-OF and ABR-RT-OR2 were used to sequence the 5’ and 3’ ends of reverse transcriptase ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmids were purified with a Midiprep kit (ZymoPURE, #D413) or a QIAprep spin miniprep kit (QIAGEN, #27104) and verified by DNA sequencing prior to use ...
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bioRxiv - Biochemistry 2023Quote: ... Negatively supercoiled pHOT-1 DNA was purified from DH5α utilizing a Qiagen Plasmid Midi Kit (Qiagen, Hilden, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were isolated from the inoculum and the m9sa cultures following selection using a miniprep kit (Qiagen, Inc.).
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bioRxiv - Biophysics 2023Quote: MCF10A-ER-src cells were transiently transfected with the CAV1 overexpressing plasmid using Effectene transfection reagent (301425, Qiagen). One day before transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA from ~ 20 positive clones for each cell line was isolated using QIAprep Spin Miniprep Kit (Qiagen) and the cloned rDNA promoter region was sequenced using Sanger sequencing ...
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bioRxiv - Plant Biology 2023Quote: All plasmid DNA isolation from bacterial cells was performed using the QIAprep Spin Miniprep Kit (Qiagen; Hilden, Germany) according to the manufacturer’s information ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was isolated using either QIAGEN Plasmid Plus Midi Kit or 28 QiaPrep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from 5 mL of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). The remaining 95 mL of culture was combined with 40.7 mL 50% glycerol (v/v) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from 5 mL of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Glycerol was added to the remaining culture to a final concentration of 15% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... coli and the plasmid DNA was extracted using the pipetting Bio Robot Universal System (Qiagen cat. no. 9001094) and the QIAprep 96 plus BioRobot kit (Qiagen cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 200 ng psiCHECK-2 reporter plasmid were co-transfected with 5 µl miScript miRNA mimics (Qiagen; CatNo. 219600) using 4 µl Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO3 (pH 8.3) ...
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bioRxiv - Genomics 2024Quote: ... each 50 mL midiprep culture was spun down for 30 minutes at 4,300g and plasmid DNA isolated using a midiprep kit according to the manufacturer’s instructions (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: ... Inserts were PCR amplified using M13 primers and template plasmid was digested with DpnI before PCR purification (Qiagen). Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega) ...
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bioRxiv - Developmental Biology 2024Quote: ... The plasmid DNAs were linearized by a restriction enzyme and purified with the Qiaquick Gel Extraction Kit (Qiagen) before microinjection ...
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bioRxiv - Microbiology 2024Quote: The isolation of DNA (GeneEluteTM Bacterial Genomic DNA, Sigmal-Aldrich) or plasmids (QIAprep® Spin Miniprep Kit, Qiagen) was performed applying commercial kits ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... The cDNA libraries were amplified in DH10B E.coli cells and purified using QIAGEN Plasmid Mega Purification kit (Qiagen, Germany). The average size of the cDNA inserts into pPR3-N was evaluated by purifying 20 random colonies each and digesting with SfiI ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 100 μg/mL of carbenicillin for 16 hr at 30°C followed by plasmid purification (Qiagen, 12991).
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmid DNA was then extracted with a custom method using reagents from the QIAprep Miniprep Kit (Qiagen cat. #27104) on an automated liquid handler equipped with a positive-pressure filter press.
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bioRxiv - Microbiology 2019Quote: ... pMo-EGFP-PV-WT plasmid was linearized with ApaI and purified with QIAEX II Gel Extraction Kit (Qiagen, Netherlands). With the linearized plasmid as the template ...
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bioRxiv - Biochemistry 2020Quote: ... The selected plasmids were purified by use of a QIAprep Spin Miniprep Kit according to the manufacturer’s instructions (QIAGEN). For the reaction products stemming from transcription with natural CTP ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We used the remainder of the culture for extracting the plasmid library using a QIAprep Spin Miniprep kit (QIAGEN). The plasmid libraries thus isolated served as templates for the next round of mutagenesis.
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bioRxiv - Immunology 2021Quote: Bacterial plasmid DNA was extracted from a 3 ml overnight culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Next ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were assembled via Gibson Assembly using standard protocols and isolated with the Qiaprep Spin Miniprep kit (Qiagen, USA) (146).
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bioRxiv - Biochemistry 2022Quote: ... Expression levels were validated from the pWB plasmid by western blot using an anti-His HRP conjugate kit (Qiagen) (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining recovery was grown overnight in 150 mL LB + chloramphenicol and plasmid DNA was isolated by MaxiPrep (Qiagen). The complexity of the SpCas9 catalytic and PAM domain libraries were estimated to be 77,400 and 292,600 respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... All selected colonies were cultured overnight and prepared using the HiSpeed Plasmid Maxi Kit (Qiagen, Hilden Germany, cat# 12663) and sequenced at Genewiz (South Plainfield NJ USA ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was isolated from select transformants using a miniprep kit according to the instructions of the manufacturer (QIAGEN), and the recombinant construct ...
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bioRxiv - Neuroscience 2020Quote: ... Endotoxin free plasmid preparations of high yield and purity for electroporation was prepared by maxi-prep (Qiagen, Hilden, Germany).
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bioRxiv - Synthetic Biology 2019Quote: ... or Golden Gate Assembly using standard protocols37 Plasmids were routinely isolated using the Qiaprep Spin Miniprep kit (Qiagen, USA), and all primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Genetics 2021Quote: The pEGFP-attB vector with the priEE fragment was prepared at a high concentration using Plasmid Midi Kit (Qiagen) and transported to WellGenetics (Taiwan ...