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Citations for Qiagen :
1451 - 1500 of 1566 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The CYP168A1 optimized cDNA insert was removed from the plasmid using NdeI and HindIII restriction enzymes and isolated using agarose gel electrophoresis and the Qiagen Gel Extraction Kit (Qiagen) for ligation into a similarly digested and isolated pCWOri+ CYP expression vector (113) ...
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bioRxiv - Genetics 2022Quote: ... DNA was extracted from a wild type clone and a mutant using a QIAGEN Plasmid Midi Kit (Qiagen, Tokyo, Japan) and used as standard DNA to determine ratios of mosaicism ...
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bioRxiv - Genetics 2022Quote: ... 3-5 positive colonies per L1 element were chosen for Miniprep culture and plasmid DNA was isolated using QIAprep Spin Miniprep Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... at a density of 1-2×106 cells/ml and transfected with pRmHA3-GAL4 [60] and HA-tagged p10UAST-Robo or untagged pUAST-Slit plasmids using Effectene transfection reagent (Qiagen). GAL4 expression was induced with 0.5 mM CuSO4 for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... 2-3 mL of each bacterial culture was pelleted and plasmid DNA extracted and purified using the QIAPrep SpinPrep miniprep kit (QIAGEN) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids were transformed into JM109 chemically competent cells and grown overnight in 250 mL of LB broth with 100 µg/mL of ampicillin and purified using the HiSpeed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... A midi prep scale of plasmid DNA (pGO10GG, 5500 bp, sequence available upon request) was purified following manufacture protocol (Qiagen). The promoter template for in vitro transcription with the human Pol II system was prepared from the native human HSPA1B gene ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was transcribed from a linearized plasmid DNA template using the SP6-Scribe Standard RNA IVT Kit (CELLSCRIPT) and purified with the RNeasy Mini Kit (QIAGEN). The m7G cap structure was added to the purified RNA using the ScriptCap m7G Capping System (CELLSCRIPT) ...
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bioRxiv - Cell Biology 2020Quote: ... The resultant plasmid DNA was linearized with PstI restriction enzyme and purified with a QIAquick PCR Purification Kit (QIAGEN, Germany). Purified template was in vitro transcribed by RiboMAX™ Large Scale RNA Production System with T7 RNA polymerase (Promega ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmid coding for FLP recombinase and the mCherry reporter or 47bp-insertion TLR reporter plasmid were transfected into the cells at a 9:1 ratio using polyfect (QIAGEN) according to the manufacturer’s protocol (900ng mCherry reporter or 47bp-insertion TLR reporter plasmid and 100ng FLP recombinase plasmid to make 1 μg plasmid in total) ...
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bioRxiv - Genomics 2020Quote: ... Both the linearized plasmid and the DNMT3L PCR amplicon were run on an agarose gel and purified in a silica column (Qiagen). Purified fragments were mixed at 1:3 molar ratio and assembled with NEBuilder HiFi DNA Assembly Mater Mix (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... and plasmids were subsequently isolated from individual colonies grown overnight in LB broth by using a mini-prep kit (Qiagen). For chlamydial transformation ...
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bioRxiv - Biochemistry 2019Quote: ... which include the Illumina adapters and the 151 bp of plasmid DNA that flanked the mismatch region were purified using the QIAquick PCR Purification Kit (Qiagen) and quantified with PicoGreen (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: pCVB3-H3-WT and pCVB3-H3-VP1-F106L plasmids were linearized with ClaI and purified with the QIAquick Gel Extraction kit (Qiagen). In vitro transcription was performed with the P1230 kit (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... Positive clones were verified by Sanger sequencing analysis and the plasmid containing the chimeric vector was purified with a QIAprep Spin Miniprep kit (Qiagen). The chimeric vector containing the f88-4 epitope 4 (aa 662–671 ...
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bioRxiv - Microbiology 2021Quote: ... coli giant spheroplasts we used a N-terminally 6-His tagged version of TcMscS cloned into the expression plasmid pQE80 (Qiagen) with restriction sites BamHI and HindIII ...
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bioRxiv - Microbiology 2020Quote: ... and plasmids were subsequently isolated from individual colonies grown overnight in LB broth by using a mini-prep kit (Qiagen). For chlamydial transformation ...
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bioRxiv - Molecular Biology 2022Quote: ... Isolated colonies were inoculated into 5 mL of LB with appropriate antibiotics and plasmids were purified using the alkaline lysis protocol and Qiaquick miniprep kit (Qiagen). Plasmids were verified by sequencing (GATC ...
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bioRxiv - Biophysics 2022Quote: ... The liquid culture was then grown for 3 hours at 37°C before the plasmid extraction using a miniprep kit (QIAGEN). Prepared DNA was sequenced by the Microbial Genome Sequencing Center (https://www.seqcenter.com/ ...
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bioRxiv - Genomics 2022Quote: ... Wolbachia density relative to host DNA and plasmid copy number were measured by qPCR using the QuantiNova SYBR Green PCR kit (Qiagen) in 10 μL reactions ...
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bioRxiv - Microbiology 2022Quote: ... Biomass was collected by centrifuging at 5,000 rpm for 30 min and fosmid DNA was extracted from the pellet using the QIAGEN Plasmid Midi Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Drosophila S2 cells were transiently transfected with pAc-brm-FLAG-6xHIS in combination with either pAc-clk-V5-HIS or pAc-V5-HIS empty plasmid using Effectene (Qiagen). For cycloheximide experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transiently transfected with pAc-brm-FLAG-6xHIS in combination with pAc-clk-V5-HIS or pAc-V5-HIS empty plasmid using Effectene (Qiagen). Cells were harvested 48 hours after transfection for processing ...
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bioRxiv - Synthetic Biology 2023Quote: ... Genomic DNA and plasmids were extracted using the Fast Pure Bacteria DNA isolation mini kit (Vazyme) and the QIAprep Spin Miniprep Kit (Qiagen), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... into parasites already ectopically expressing NLS-mCherry (previously provided by Markus Ganter (Simon et al., 2021a)) with 50–100 μg of purified plasmid DNA (QIAGEN). Plasmid precipitation was done prior to transfection as stated above ...
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bioRxiv - Biophysics 2022Quote: ... The AbundancePCA plasmid and BindingPCA plasmids were all digested by HindIII and NheI enzymes and purified using the QIAquick Gel Extraction Kit (QIAGEN). The assembly of AbundancePCA libraries and BindingPCA libraries were done overnight by temperature-cycle ligation using T4 ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 ng of the plasmids with the intronic fragments were expressed into the S2 cells with Effectene Transfection Reagent from Qiagen. For dFOXO ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were subsequently purified via overnight culture followed by purification via a commercial endotoxin-free spin midi prep kit (Qiagen).
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bioRxiv - Biophysics 2023Quote: ... The following day we performed three more rounds of plasmid clean-up using the same QIAprep Spin Miniprep kit (Qiagen) to remove all the residual enzymes which could corrupt the AFM images.
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bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 37°C overnight in presence of a selective antibiotic Chloramphenicol (30 µg/ml) and isolated the final plasmid using a QIAprep Spin Miniprep kit (Qiagen) the following day ...
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bioRxiv - Neuroscience 2023Quote: ... and colonies yielding an ≈2.4 kB product were isolated from a duplicate patch plate and grown in liquid culture as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Biochemistry 2023Quote: ... were used as per manufacturer’s instructions to clean up the reacted plasmid DNA and eluted in a 50 μL volume in buffer EB (Qiagen).
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bioRxiv - Neuroscience 2023Quote: ... Ampicillin-resistant colonies were selected from transformation plates and liquid bacterial cultures grown as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Microbiology 2023Quote: ... 50% confluent HEK 293T cells were transfected with 6000 ng GFP-tagged RhCMV gH-CD4 TM plasmid using Effectene Transfection Reagent Kit (Qiagen) next day ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding the recombinant RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). On Day 0 ...
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bioRxiv - Biophysics 2023Quote: ... WT and mutant DNA plasmids were linearized by HindIII-HF or EcoRI-HF and purified with the QIAquick PCR Purification Kit (QIAGEN). cRNA was transcribed in vitro with the mMESSAGE mMACHINE T7 kit (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
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bioRxiv - Genetics 2022Quote: ... The linearized plasmid backbones and PCR products were analysed on agarose gels and purified using the QIAquick gel extraction kit (Qiagen). Assembly of DNA fragments was either performed with isothermal assembly or Goldengate-like reactions (see details on individual plasmids below) ...
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bioRxiv - Biochemistry 2023Quote: ... Up to three resistant colonies per mutation were selected and plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen) and quantified using a nanodrop spectrophotometer (ND.1000) ...
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bioRxiv - Biochemistry 2023Quote: ... A linearized PCR template was generated by digesting a wild-type Hrd1 plasmid with restriction enzyme and purified using a QIAquick PCR purification kit (Qiagen). For each region two separate PCR reactions were set up ...
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bioRxiv - Immunology 2023Quote: ... Plasmid DNA from overnight liquid cultures (LB with 100 mg/ mL ampicillin) was isolated with QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Developmental Biology 2023Quote: ... at a density of 1-2×106 cells/ml and transfected with pRmHA3-GAL4 (Klueg et al., 2002) and HA-tagged p10UAST-Robo or untagged pUAST-Slit plasmids using Effectene transfection reagent (Qiagen). GAL4 expression was induced with 0.5 mM CuSO4 for 24 hours ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from five 10-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from ten 4-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Neuroscience 2024Quote: ... Overnight cultures of the positive clones were grown in LB Kanamycin (30 μg/mL) media and plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (Qiagen). Purified plasmid DNA was sequenced using ABI BigDye Terminator v3.1 Sequencing at the Australian with the following primers ...
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bioRxiv - Physiology 2024Quote: ... and NR4A3-overexpressing (NR-203Oex) plasmid DNA was then purified using endotoxin-free buffers (Cat. No. 19048) and columns (Cat. No. 10083) from Qiagen, before assessing DNA concentration and purity by spectrophotometry.
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bioRxiv - Neuroscience 2023Quote: ... primary hippocampal neurons were transfected in duplicates or triplicates using 150ng of a plasmid carrying enhanced GFP-gene in combination with 5nmol Power Lock-Nucleic Acid inhibitors (Qiagen) against miR-218-5p ...
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bioRxiv - Microbiology 2023Quote: ... were cotransfected with 0.35 μg of psPAX2 or pNL(AD8) and 0.45 μg of a reporter plasmid using Effectene transfection reagent (Qiagen, USA). The next day ...
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bioRxiv - Microbiology 2023Quote: ... pHIVec2.luc reporter plasmid and psPAX2 packaging plasmid (catalog number 11348, NIH AIDS Reagent Program) in a ratio of 1:6:3 using either Effectene (Qiagen) or calcium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... shaking overnight at 30°C for 16hrs and the plasmid DNA is purified using a QiaPrep 96 column DNA purification kit (Qiagen). This procedure was conducted twice on different days to produce two separate replicates.