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Citations for Qiagen :
1251 - 1300 of 1566 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and plasmid DNA of positive clones was obtained using QIAprep Spin Miniprep Kit (Qiagen, Cat#: 27106). Clones were verified as mutation-free by capillary sequencing ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 million cells were seeded and transfected with 1μg pMK33-based plasmid DNA using Effectene (Qiagen). After 48 hours ...
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bioRxiv - Microbiology 2019Quote: ... The plasmids were extracted from these positive bacterial colonies (QIAprep Spin Miniprep Kit, Qiagen, Hilden, Germany) and submitted for bidirectional ...
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bioRxiv - Molecular Biology 2019Quote: ... pGEMT-UnFosb or pGEMT-Fosb) and reference plasmid (pGEMT-Gapdh) using Rotor-gene Q software (Qiagen). Primer sequences are found in Supplementary Table 3.
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bioRxiv - Cancer Biology 2020Quote: ... All plasmids used for in vivo experiments were purified using the Endotoxin-free Maxiprep kit (Qiagen). [U-13C]-glucose ...
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bioRxiv - Developmental Biology 2019Quote: ... All DNA plasmids used in electroporation experiments were purified using Midiprep DNA isolation kits (Qiagen, 12143) and resuspended in Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2020Quote: Expression plasmids: PCR fragments of the expected sizes were extracted using a gel extraction kit (Qiagen) and cloned in pGEM-T easy vector (Promega) ...
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bioRxiv - Synthetic Biology 2021Quote: ... After 16-24h (~ 70% confluency) cells were transfected with 300ng of plasmid mix using Effectene (Qiagen) and following manufacturer instructions for adherent cell cultures ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was extracted from 10 ml overnight culture using QIAprep® Spin Miniprep Kit (QIAGEN) with the following adaptations ...
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bioRxiv - Bioengineering 2020Quote: ... coli bacteria (as described in section 2.1) and extracted using a Plasmid Maxi kit (Qiagen, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid isolation and genomic DNA preparations were carried out using the QIAprep Miniprep kit (Qiagen, UK) and DNeasy Blood & Tissue kit (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... coli O157:H7 EDL933 genome (NCBI Accession Numbers: chromosome, NZ_CP008957.1; pO157 plasmid, NZ_CP008958.1) using CLC Genomics Workbench (Qiagen) with default parameters ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA purification was later performed for these pelleted cells using the Plasmid Plus Midi Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Cells were co-transfected with the different plasmids using Effectene reagent according to manufacture indications (Qiagen). For stable transfection ...
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bioRxiv - Molecular Biology 2022Quote: Plasmid DNA was isolated using the QIAprep Spin Miniprep Kit following the manufacturer’s protocol (QIAGEN, 27104) and eluted in water ...
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bioRxiv - Microbiology 2022Quote: ... Preparation of plasmid and purification of PCR products was done by designated Qiagen kits (Qiagen, Germany). Oligonucleotides were purchased from a commercial source (Bioserve Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... endotoxin-free DNA prepared at 400 ng/μl using Qiagen Plasmid Plus Midi Kit (Qiagen 12945). For live-cell imaging of exocytosis ...
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bioRxiv - Physiology 2023Quote: ... Plasmids containing the ABG sequences from each individual were then mini prepped (27106X4, Qiagen, Hilden, Germany) and sanger sequenced with M13F and M13R primers (Azenta Life Sciences ...
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bioRxiv - Systems Biology 2023Quote: ... after which we pelleted cultures by centrifugation and used the EndoFree Plasmid Maxi Kit (Qiagen #12362) to isolate plasmid according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: Plasmid DNA from bacterial cells was isolated using the QIAprep Spin Miniprep Kit (Qiagen; Hilden, Germany) according to the manufacturer’s information ...
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bioRxiv - Microbiology 2022Quote: 1.5×105 Acanthamoeba castellanii cells were transfected with 6 μg of linearized plasmid using Polyfect (QIAGEN) in phosphate saline buffer (PBS) ...
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bioRxiv - Microbiology 2022Quote: 1.5×105 Acanthamoeba castellanii cells were transfected with 6 μg of each plasmid using Polyfect (QIAGEN) in phosphate saline buffer (PBS) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid DNA was purified from E.coli host strains using the QIAprep Spin Miniprep kit (Qiagen 27106). Plasmid DNA was quantitated using a NanoDrop spectrophotometer and diluted to 20 fmol/μL in nuclease-free water (VWR 02-0201-0500) ...
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bioRxiv - Genetics 2022Quote: ... Tol2 transposase mRNA was transcribed from the plasmid pCS-TP with the mMessage mMachine kit (Qiagen).
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bioRxiv - Biochemistry 2022Quote: The fosmid DNA of the positive clone was extracted using the QIAGEN Plasmid purification kit (QIAGEN). To reduce the host chromosomal E ...
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bioRxiv - Biophysics 2023Quote: ... All constructed plasmids were prepared from XL1-Blue cells using the QIAprep Spin Miniprep Kit (QIAGEN). Protein-coding sequences were verified by Sanger sequencing at UC-Berkeley DNA Sequencing Facility ...
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bioRxiv - Biochemistry 2023Quote: I21 WT and C3575S cDNAs were cloned in a custom made pQE80-based expression plasmid (Qiagen) using BamHI and BglII restriction enzymes (sequences available in Supplementary File S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was isolated from the cultured bacteria using a QIAprep Spin Miniprep Kit (Qiagen # 27106). Plasmid DNA was sent to Genewiz for sequencing using the CMV-Forward and BGHR primers ...
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bioRxiv - Bioengineering 2023Quote: ... after which we pelleted cultures by centrifugation and used the EndoFree Plasmid Maxi Kit (Qiagen #12362) to isolate plasmid according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Negatively supercoiled pBR322 DNA was purified from DH5α utilizing a Plasmid Mega Kit (Qiagen, Hilden, Germany). Negatively supercoiled pHOT-1 DNA was purified from DH5α utilizing a Qiagen Plasmid Midi Kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Single colonies were isolated and further expanded in LB broth before plasmid purification with miniprep (Qiagen) following the manufacturer protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were prepared by mini or maxi preps using commercially available kits (Qiagen and Promega, respectively) following manufacturers’ instructions ...
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bioRxiv - Genetics 2023Quote: Bacterial colonies were grown up and the plasmids were extracted using a kit (Qiagen, Germantown, MD). The samples were sent for sequencing with KAR4-specific primers ...
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bioRxiv - Microbiology 2023Quote: ... RNA was in vitro transcribed from linearized NotI digested plasmid and purified using RNeasy kits (Qiagen). Two micrograms RNA was transfected into Vero cells using Lipofectamine LTX (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... the wash was repeated by using 0.7 mL PE buffer (From QIAGEN Plasmid Plus Midi Kit). After washing ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid DNA was purified from the cultures using the QIAprep Spin Miniprep Kit (Qiagen, Germany) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... We prepared plasmid by using QIAprep Spin Miniprep Kit from Qiagen (Cat.No.27104 and Cat.No.27106). Plasmid was sequenced by using M13 R vector specific primers and analyzed the sequence and identified CpG sites by using bioinformatics software tools such as Vector NTI ...
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bioRxiv - Systems Biology 2023Quote: ... The cleared supernatant was applied to columns provided in the Plasmid Midi Kit (QIAGEN, Düren, Germany) that had been equilibrated with 10 mL buffer QBT ...
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bioRxiv - Cancer Biology 2023Quote: ... Bacterial stubs were cultured and maxi-prepped using the QIAGEN Plasmid Plus Maxi Kit (Qiagen 12963) and as per manufacturer’s directions ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells were transfected with 1 μg of pUbg-R252 plasmid using Effectene transfection reagent (QIAGEN) in a 60 mm dish following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... These cells were transiently transfected plasmids as described below using either Effectene (Qiagen, Cat. Nu. 301425) or PEI-Max (Polysciences Inc ...
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bioRxiv - Systems Biology 2020Quote: ... The plasmids were bought in lyophilized form (Arbor Biosciences) and purified using QIAprep Spin Miniprep Kit (Qiagen) using cell lines DH5-Alpha (for P28a-cI-ssrA ...
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bioRxiv - Genetics 2021Quote: ... DNA was extracted from 50 ml LB from an overnight culture using a plasmid midi kit (Qiagen). Successful genomic DNA extraction was confirmed by PCR with primers gp41 inner F (5’-CAAGAGCAAAGAACCGACG-3’ ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK-293T cells transfected with the desired plasmids by using Attractene Transfection Reagent (301005, QIAGEN, Hilden, Germany), according to the fast-forward protocol ...
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bioRxiv - Genetics 2021Quote: ... All TALE-VP64 and TALE- Δ plasmids were miniprepped using the QIAprep Spin Miniprep kit (Qiagen 27104) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 mL were collected and used for plasmid extraction using a QI-Aprep Spin Miniprep kit (QIAGEN). Extracted plasmids were eluted in 30 μL of elution buffer and stored at −20 °C until use.
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bioRxiv - Developmental Biology 2021Quote: ... plasmid encoding Cas9 and gRNA or WT control organoids using QIAamp Fast DNA Tissue Kit (51404, Qiagen). PCR was performed using PrimeSTAR® GXL DNA Polymerase (R050A ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were isolated from 5 mL of overnight liquid culture using QIAprep Spin Miniprep Kit (Qiagen, Germany) and the presence of mutations was confirmed by Sanger Sequencing.
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bioRxiv - Cell Biology 2021Quote: ... CRISPR/Cas9 and donor plasmids were co-transfected into the cell lines using Effectene (Qiagen, Venlo, Netherlands). For drug selection ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA used in transfection of cells was purified using the Qiagen MaxiPrep kit (Qiagen, Valencia, CA).