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Citations for Qiagen :
1151 - 1200 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted in 96-well format from 100 μL of sample using the MagAttract PowerMicrobiome DNA/RNA Kit (Qiagen, Hilden, Germany) automated on a Hamilton Microlab STAR robotic platform ...
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bioRxiv - Plant Biology 2020Quote: ... 100-150 ng/μL of DNA was extracted from 100 mg of ground leaf tissue using DNeasy Plant Mini Kit (Qiagen Inc., Canada) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... High-quality genomic DNA was extracted from about 100 mg of frozen leaf material using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany), following standard protocols ...
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bioRxiv - Evolutionary Biology 2021Quote: ... For the detection of phosphorylated Serine residues a TBS solution with a 1:100 dilution of the Anti-Phospho-Serin Antibody (Qiagen, Hilden, Germany) was used ...
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bioRxiv - Genetics 2021Quote: ... according to Protocol I supplied by the manufacturer or the QIAGEN Blood & Cell Culture Kit with 100/G or 20/G Genomic-tips (Qiagen, Hilden, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA was prepared from 100 ORw1118 adult female flies for each biological replicate (three biological replicates in total) with Gentra Puregene Cell kit (Qiagen cat#158388) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was isolated from 100 to 300 µl nematode pellets using the Blood and Tissue DNA isolation kit (cat# 69506, QIAGEN, Valencia, CA) following established protocols (Cook et al ...
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bioRxiv - Genomics 2021Quote: A half-loopful overnight grown bacterial culture was used to extract the genomic DNA using the QIAGEN Genomic-tip 100/G (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We extracted DNA following the Dneasy Blood & Tissue kit standard procedure and we performed twice the final elution in 100 µl of AE buffer (QIAGEN, Hombrechtikon, Switzerland). We quantified the extracted DNA using Qubit® 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: DNA was isolated from the swab and tissue samples using a Qiagen PowerSoil DNA Isolation kit (Qiagen, Hilden, Germany; cat. # 12888-100) following the manufacturer’s protocol (23) ...
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bioRxiv - Microbiology 2022Quote: ... 2 mL of 100% ethanol was added to each sample and RNA extraction was performed using the Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: cDNA was prepared using 100 ng RNA from FANS-purified GFP+ OPC nuclei or from optic tracts (RNeasy Mini kit; Qiagen Cat# 74104) and 1.5 μL of 100 ng/L of random hexamers (Integrated DNA Technologies Cat# 51-01-18-26 ...
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bioRxiv - Microbiology 2023Quote: ... where reactions were filled to 100 μl with nuclease-free water and buffer RLT from RNeasy Mini Kit (Qiagen, Venlo, the Netherlands) was added ...
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bioRxiv - Microbiology 2023Quote: ... Cytokine and autoantibody levels were measured using the Luminex 100 system (Austin, TX, USA) and subsequently analyzed by using LiquiChip Analyzer Software (Qiagen, Hilden, Germany).
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bioRxiv - Synthetic Biology 2023Quote: DNA extraction of root and rhizosphere samples was performed using Qiagen DNeasy Powersoil DNA extraction Kit (Catalog No. 12888-100; QIAGEN, Venlo, Netherlands) with 150 mg of root and 200 mg of rhizosphere as starting material in the provided Powersoil collection vials and then following the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2023Quote: Total viral genomic DNA was extracted from 100 μL stored plasma samples with the QIAamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany), according to manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used 100 mg of each dried sample for DNA extraction following the Qiagen DNeasy Plant Mini Kit protocol (Qiagen, Hilden, Germany). The extractions’ quality and concentration were evaluated using GelRed 1.5% agarose gels and a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted the genomic DNA of each fin clip following the DNeasy Blood and Tissue kit standard procedure and performed the final elution twice in 100 µl of AE buffer (QIAGEN, Hombrechtikon, Switzerland). We quantified the extracted DNA using Qubit® 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from approximately 100 mg of frozen stem tissues for each sample using the Qiagen RNeasy Plant Mini-Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: We extracted the genomic DNA of each fin clip following the DNeasy Blood and Tissue kit standard procedure and performed the final elution twice in 100 µl of AE buffer (QIAGEN, Hombrechtikon, Switzerland). We quantified the extracted DNA using Qubit® 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... cfDNA was extracted from approximately 4□ml of plasma (QIAamp Circulating Nucleic Acid kit, Qiagen) and then constructed into sequencing libraries with end repair ...
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bioRxiv - Microbiology 2020Quote: ... Total DNA was extracted from 1 ml liquid samples using DNeasy Blood & Tissue kit (Qiagen). The variable ends of subtype II-C and type VI-B loci (C1 and C2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and gonad were collected and stored separately in 2-mL tubes containing RNA later (QIAGEN) overnight at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... proteins were purified by batch Ni-NTA bead purification (1 mL slurry/1L culture; Qiagen) and further purified by a Superdex 200 16/60 column (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... cfDNA was extracted from 2 ml of plasma using QIAamp Circulating Nucleic Acid Kit (QIAGEN) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... and the supernatant was applied to 2 mL of pre-washed Ni-NTA resin (Qiagen) at 4°C ...
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bioRxiv - Genomics 2020Quote: ... The supernatant of cell lysate was incubated with 1 mL Ni-NTA agarose beads (QIAGEN) at 4 °C for 1 h ...
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bioRxiv - Genomics 2019Quote: ... cfDNA was extracted from 1 or 4 ml of urine according to manufacturer recommendations (Qiagen Circulating Nucleic Acid Kit ...
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bioRxiv - Microbiology 2019Quote: ... eight milliliters of culture was added to 8 ml of RNA Protect bacteria reagent (Qiagen) in 50-ml sterile tubes and then vortexed immediately for 5 sec and incubated at room temperature for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... Co-NTA beads were obtained from stripping 1 mL of Ni-NTA resin from Qiagen in a Bio-rad gravity column with 50 mL of 0.5M EDTA (pH 8.0) ...
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bioRxiv - Biochemistry 2021Quote: ... and the membrane suspension was mixed with 1 ml of Ni-NTA Superflow resin (Qiagen) per 1mg of GFP–His8 and incubated for 3 hours at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... coli* culture from each growth condition were stabilised in RNAProtect Bacteria Reagent (2 mL; Qiagen) and cells pellets were frozen at −80°C prior to processing ...
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bioRxiv - Bioengineering 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4 °C for 2 h with rotation.
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bioRxiv - Biochemistry 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4°C for 2 h with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... 4 °C) and the supernatant was then loaded onto 1 ml HisTrap HP column (Qiagen) at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris were eliminated by centrifugation and 1 mL of Ni-NTA superflow beads (Qiagen) was added to bind his-tagged proteins ...
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bioRxiv - Microbiology 2021Quote: ... The soluble cell lysate fraction was loaded on a 4 mL Ni-NTA column (QIAGEN) pre-equilibrated with binding buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse cross-linking was performed by the addition of 0.2 mg ml-1 RNaseA (Qiagen) and 0.2 mg ml-1 Proteinase K (Promega) ...
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bioRxiv - Biophysics 2021Quote: ... and the supernatant was incubated with ∼1.0 ml of Strep-Tactin Superflow Plus resin (QIAGEN) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: The supernatant was mixed with 1 ml resin volume of Ni-NTA beads (Qiagen, 30210) which was pre-equilibrated with Lysis buffer supplemented with 40 mM imidazole and 0.1 mM ATP ...
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bioRxiv - Evolutionary Biology 2021Quote: ... suspended in 15 mL AP1 buffer from a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany), and centrifuged (500 × g ...
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bioRxiv - Genomics 2022Quote: ... Lysate was spun down and supernatant was mixed with 2 ml Ni-NTA resin (Qiagen) per 50 ml sample and incubated for 1h at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... the supernatant was purified by 1 mL Ni2+ IMAC with Ni-NTA Superflow resins (Qiagen). Resins with bound cell lysate were washed with 10 mL (bed volume 1 mL ...
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bioRxiv - Microbiology 2020Quote: ... homogenized in 1 ml of PBS with a steel ball by a Tissue Lyser (Qiagen) at 25 Hz for 1 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the mixture was left to incubate in batch with 1 mL Ni-NTA resin (QIAGEN) overnight at 4°C ...
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bioRxiv - Genomics 2019Quote: ... 9.5 mL Qiagen Buffer G2 from the Genomic DNA Buffer Set (Cat. No. 19060, Qiagen), 19 μL RNase A (100mg/mL ...
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bioRxiv - Genomics 2021Quote: ... Then 37.5μl of beta-mercaptoethanol (0.375% final) and 10μl RNAse A (Qiagen® 100mg/mL) were added ...
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bioRxiv - Biochemistry 2020Quote: ... the eluted fraction was subsequently passed through a 5 ml Ni-NTA Superflow cartridge (Qiagen). The column was washed with 10 CV Ni-NTA Wash Buffer 1 (PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was then batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin at 4°C for 1 hour in a beaker set on a stir plate ...
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bioRxiv - Biochemistry 2021Quote: ... The protein solution was subsequently applied to a 5 ml Ni-NTA Superflow cartridge (Qiagen) which was washed with dialysis buffer supplemented with increasing concentrations of imidazole (10-250 mM) ...