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Citations for Qiagen :
951 - 1000 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Expressed proteins were subsequently purified by Ni-chelate chromatography (Qiagen, 5 ml column) and SEC (S75 ...
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bioRxiv - Biochemistry 2019Quote: ... The filtered supernatant was loaded onto a Ni2+ -chromatography column (5 ml, Qiagen) and washed in 20 CV buffer (50 mM Tris ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the plugs were treated with RNase (Qiagen, 20µl in 1 ml TE buffer) for 1h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and digested with protease K in a concentration of 20 mg/ml (Qiagen). After several washing steps ...
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bioRxiv - Neuroscience 2020Quote: ... weighed and homogenized in 1 ml of DMEM with stainless steel beads (QIAGEN) for 30s at 30 Hz using TissueLyserII (QIAGEN) ...
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bioRxiv - Biochemistry 2020Quote: ... The cell lysate was applied on 2 ml of Ni-NTA agarose (QIAGEN) equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was then loaded onto 3 ml of Ni-NTA resin (Qiagen) by gravity at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Nhp6 was pulled down with 0.5 ml packed Ni-NTA agarose beads (Qiagen) for 3 hours at 4°C with gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was mixed with 1 mL of Ni-NTA agarose beads (Qiagen), and the mixture was rotated at 4 °C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was incubated with 2.0 ml of Ni-NTA resin (Qiagen, 30210) for 1 h at 4°C with rotation and the resin was applied to a column ...
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bioRxiv - Neuroscience 2020Quote: Antennae from twenty moths were homogenized in 1 mL QIAzol (Qiagen, Hilden, Germany). RNA was extracted using Qiagen RNeasy Mini kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were then incubated with 1 mL prewashed Ni-NTA resin (Qiagen) at 4 °C for 2 h on a rotator ...
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bioRxiv - Microbiology 2020Quote: ... The cell pellets were resuspended in 1 ml Qiagen RNA protect reagent (Qiagen) and incubated for 24 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: 2 □ 107 spores were mixed with 1 ml RNAlater RNA stabilization reagent (Qiagen) and centrifuged at 17,000 g for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed with 3 column volumes of lysis buffer and proteins were eluted stepwise with lysis buffer supplemented with 50 and 250 mM of imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The cleavage products were passed through a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with 5 column volumes of lysis buffer supplemented with 50 mM imidazole to remove the cleaved tag and the TEV protease ...
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bioRxiv - Immunology 2020Quote: ... coli in LB Broth with 100ug/mL ampicillin using Qiaprep Spin columns (Qiagen).
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bioRxiv - Biochemistry 2021Quote: ... peritoneal membrane sections (80 mg) were dissociated in 1 ml buffer RLT (QIAGEN) supplemented with β-mercaptoethanol (1:100 v:v ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was then immediately added to 1 mL RNAprotect Bacteria Reagent (Qiagen), vortexed ...
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bioRxiv - Biophysics 2020Quote: ... This periplasmic extract was then incubated with 5 ml Ni-NTA beads (Qiagen) at 4°C for 15 minutes and the resin slurry was packed on an open chromatography column ...
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bioRxiv - Cell Biology 2022Quote: Aliquots (1-1.2 ml) of culture were added to RNAProtect Bacteria Reagent (Qiagen) (2-2.4 ml) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was isolated from the lysate using 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer and then washed with 15 column volumes (CV ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed in 1 mL of RNAprotect solution (Qiagen, Hilden, Germany), pelleted and stored at −80°C until gDNA extraction ...
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bioRxiv - Immunology 2021Quote: ... with 10 μL/mL β-mercaptoethanol and mechanically disrupted with a TissueRuptor (Qiagen), before RNA extraction ...
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bioRxiv - Physiology 2020Quote: ... Erythrocytes were lysed for 3 minutes with 3 ml of EL buffer (Qiagen). After Fc blocking for 20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer then eluted in buffer containing 250 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied to three 5 ml Ni-NTA Superflow cartridges (Qiagen). The resin was washed with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to a 5 ml Ni-NTA Superflow cartridge (Qiagen) in order to remove the uncleaved fusion protein and His6-TEV protease ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Genomics 2021Quote: ... 1XPIC) with or without 0.1l of 100mg/ml RNaseA (Qiagen cat. no. 19101) for 15 min on ice briefly tapped few times during incubation time and then centrifuged at 1000g for 5 min at 40C ...
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bioRxiv - Microbiology 2019Quote: ... and the bacterial pellets resuspended in 1 ml RNA Protect Bacterial Reagent (Qiagen). Following another centrifugation cycle ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied to a 1 mL of Ni-NTA Agarose (Qiagen) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied to a 1 mL of Ni-NTA Agarose (Qiagen) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 14 and 22 mg/mL for initial screening using commercially available screens (Qiagen) by sitting-drop vapor diffusion using a Crystal Gryphon robot (Art Robbins Instruments) ...
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bioRxiv - Molecular Biology 2021Quote: ... Filters were stored in 1.5 mL tubes with tissue lysis buffer ATL (QIAGEN) and proteinase K (QIAGEN) ...
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bioRxiv - Microbiology 2020Quote: ... in a 2 mL centrifuge tube for 3 min using a TissueLyzer (Qiagen). The obtained mixture was serially diluted in 5.0 mL sterile phosphate buffer and 150 µL aliquots of 10−4 to 10−7 dilutions were spread onto plates containing a range of media ...
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bioRxiv - Genomics 2021Quote: ... >300mAU (500μL of >600 mAU/ml solution) Proteinase K (Qiagen Cat No. 19131) was added to the sample and incubated at 55 °C for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was isolated from four ml of overnight culture (DNeasy kit, QIAGEN).
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was loaded on a 10 ml Ni-NTA Superflow column (QIAGEN), washed with 7 column volumes of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with 50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... The cleaved protein was passed over a 1 mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Molecular Biology 2023Quote: The eluted protein was mixed with 1 ml Strep-Tactin Superflow agarose (Qiagen) and incubated at 4 °C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from 5 ml samples using the miRNAeasy mini kit (Qiagen), treated with TURBO DNase (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... The media was then incubated with 1.5 mL of Ni-NTA beads (Qiagen) at 4°C on a rolling bed for 1 hr ...
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bioRxiv - Molecular Biology 2022Quote: ... 400 cotyledons were ground in 2 ml tubes using a Tissue Lyser (Qiagen) for 2 x 1 min at 30 Hz ...
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bioRxiv - Molecular Biology 2022Quote: ... The cleared lysate was incubated with 0.25 ml of Ni-NTA beads (Qiagen) for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was passed through 2 mL of Ni-NTA beads (Qiagen), equilibrated with buffer [50 mM Tris-HCl ...