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Citations for Qiagen :
1301 - 1350 of 1989 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The filtered lysate was then passed over a 5 mL Ni-NTA Superflow column (Qiagen). Protein elution was carried out with a gradient of buffer B ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1% (v/v) β-mercaptoethanol was mixed with 5 mL Ni-NTA resin (Qiagen) for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining lysates were incubated with 1 mL of Ni-NTA agarose (Qiagen, 1018244) on nutator (Daeiltech ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was loaded onto a 5-mL column of Ni-NTA agarose (Qiagen, Inc.) pre-equilibrated with buffer A ...
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bioRxiv - Plant Biology 2019Quote: ... Total genomic DNA was extracted from 100 mg of fresh or frozen leaf tissue using the Qiagen DNeasy Plant Mini Kit (Qiagen, Valencia, California, USA) or a modified version of the CTAB protocol of Kubisiak et al ...
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bioRxiv - Cell Biology 2019Quote: ... The tissue was ground in liquid nitrogen and 100 mg of the resulting powder used for RNA extraction as per manufacturer’s instructions (Qiagen RNeasy kit, Cat # 74904). The extracted RNA was treated with amplification-grade DNase I (ThermoFisher ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Brain parts were stored in 100 µL of Trizol™ in -80 °C for later RNA extraction using RNAeasy Mini Extraction Kit™ (Qiagen, Germany) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2021Quote: ... DNA from 100 mg of the inoculated and uninoculated residual soil samples were extracted using the PowerSoil® DNA Extraction kit (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2019Quote: ... The PCR mixture contained 0.2 μM of each primer and 40 ng of bacterial DNA in a total volume of 100 μL (HotStar Taq Master Mix Kit, Qiagen, Valencia, CA, USA) (60).
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted from approximately 100 mg of Arabidopsis roots or shoots using the RNeasy Plant Mini Kit (Qiagen, Cat. No. 74904). RNA samples were treated with DNaseI (Qiagen ...
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bioRxiv - Microbiology 2024Quote: Bacterial genomic DNA was extracted separately from caecal tissue (∼100 mg) and caecal contents (∼200 mg) using a QIAamp Fast DNA Stool Mini kit (QIAGEN, Valencia, CA, USA) following the manufacturer’s pathogen detection protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The frozen tissue samples (∼ 100 mg weighed into a tube with fresh weight recorded) were homogenized in a TissueLyser II bead mill (Qiagen; Cat. No. 85300) for 1 min at 20 Hz without thawing out ...
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bioRxiv - Microbiology 2024Quote: ... Viral DNA was purified and extracted from 100 μL of DNase I-treated culture supernatant using a QIAamp DNA Blood Mini Kit (QIAGEN GmbH, Hilden, Germany). To quantify viral DNA copies ...
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bioRxiv - Cell Biology 2019Quote: ... The mixture was washed 20x with 20 ml GST washing buffer on a disposable column (QIAGEN) The GST-fused protein was eluted with 1 ml GST elution buffer and the eluted sample was dialyzed overnight at 4°C in 2 l 1x PBS in a Slide-A-Lyzer Dialysis Cassette G2 10 000 MWCO (Thermo Scientific).
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bioRxiv - Genomics 2021Quote: ... Lysis buffer (9.5 mL of G2 buffer from the Blood & Cell Culture DNA Midi Kit (QIAGEN) and 19 µL of RNase A (100 mg/mL ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from 4 mL of culture using the miRNAeasy Mini Kit (Qiagen, Hilden, Germany) and Qiazol lysis reagent ...
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bioRxiv - Microbiology 2021Quote: ... except that 2 mL prefilled PowerBead tubes (glass beads, 0.1 mm; Cat no. 13118-50, Qiagen) were used for the bead beating ...
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bioRxiv - Genomics 2020Quote: ... We subjected samples to a two day 0.5 EDTA and proteinase K (10 mg/mL; Qiagen) digestion ...
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bioRxiv - Immunology 2021Quote: Snap frozen tissue samples or cells were homogenized in 0.5-1 ml Qiazol (Qiagen, Hilden, Germany) using a Retsch Homogenizer (Retsch GmbH ...
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bioRxiv - Immunology 2021Quote: ... the supernatant/Sepharose bead slurry was passed through a 5 ml polypropylene gravity flow column (Qiagen). The column was washed with 1 column volume of PBS before being eluted with 9 ml of Elution Buffer (0.1M Glycine/HCl buffer ...
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bioRxiv - Genetics 2019Quote: ... for lysis in 2 ml safe-lock tubes containing one 5 mm stainless steel bead (Qiagen) for 2.5-3 minutes at 30 Hz using TissueLyzer II (Qiagen) ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples (15-30 mg) were transferred to 2 ml round-bottom tubes (Qiagen, Hilden, Germany) with the addition of a 5 mm stainless steel bead (Qiagen ...
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bioRxiv - Systems Biology 2020Quote: ... Cell pellets were transferred to 2 ml polypropylene tubes and disrupted using a TissueLyser II (Qiagen), adding the same volume of acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... All wells were then pooled and diluted 5:1 (∼24 mL) in Buffer PB (Qiagen #19066) with 1/20th volume of 3 M NaOAc pH 5.2 (∼1.2 mL) ...
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bioRxiv - Cancer Biology 2020Quote: Plasmid was extracted from 1.5 ml of bacterial culture with the QIAamp DNA Mini Kit(Qiagen) and purified for sequencing validation (1st BASE) ...
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bioRxiv - Plant Biology 2020Quote: ... Pooled eluates were transferred together with 1.2 mL of Ni-NTA resin (Qiagen, Valencia, CA, USA) into a 15 mL Falcon tube and incubated for 2 h at 4°C with gentle rotation ...
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bioRxiv - Biochemistry 2020Quote: ... The cytosolic supernatant was incubated and stirred with 1 ml Ni-NTA agarose (Qiagen, Hilden, Germany) at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... and the resultant supernatant was mixed with 2 ml of Ni-NTA agarose resin suspension (Qiagen) and incubated on a rotary shaker for 60 min at 4°C before transfer to a Poly-Prep Chromatography Column (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen tissue was thawed and immediately homogenized in 2 ml of RTL buffer + 2mM DTT (Qiagen). mRNA was extracted from 300 ul of homogenate using the RNeasy Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was bound to a 2 mL slurry of Strep-Tactin Superflow plus (Qiagen) overnight at 4°C using the batch method ...
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bioRxiv - Biochemistry 2022Quote: ... Protein extract was subjected to IMAC by incubation with 2 ml Ni-NTA resin (Qiagen, Germany) per 50 ml of extract ...
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bioRxiv - Microbiology 2022Quote: ... The soluble extracts were mixed for 30 min with 3 ml of Ni2+-NTA-agarose (Qiagen) that had been equilibrated with buffer A containing 10 mM imidazole ...
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bioRxiv - Biophysics 2022Quote: ... The cleared extract was bound to 1.0 mL (bed volume) of Ni-NTA Superflow resin (Qiagen) at 4 °C for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The flow-through was collected and applied to 0.4 mL volume Ni-NTA Agarose resin (Qiagen) to remove the 7×His-TEV protease ...
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bioRxiv - Genomics 2022Quote: ... 10 mL chilled Qiagen Buffer C1 (from the Qiagen Genomic DNA Buffer Set, Qiagen no. 19060) and 30 mL chilled ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was collected and tumbled with 1 mL of preequilibrated Ni(II)-NTA Superflow (Qiagen) resin for 1-1.5 h at 4 °C.
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bioRxiv - Biochemistry 2019Quote: ... The soluble fraction was loaded onto a column containing 5 ml Ni-NTA superflow resin (Qiagen), pre-equilibrated with buffer A ...
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bioRxiv - Immunology 2020Quote: ... The bacterial lysates were mixed for 1 h with 5 ml of Ni-NTA resin (Qiagen) that had been equilibrated with buffer A ...
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bioRxiv - Microbiology 2019Quote: ... and the obtained pellets were transferred immediately into 0.5 ml of RNA Protect Bacteria Reagent (Qiagen). Cells were then incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was extracted from 10 ml overnight culture using QIAprep® Spin Miniprep Kit (QIAGEN) with the following adaptations ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was then incubated with 2 ml Ni-NTA Superflow resins (Cat No.30430, QIAGEN) for 30 minutes ...
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bioRxiv - Genomics 2021Quote: cfDNA was extracted from 2-4 mL of plasma using the QIAsymphony liquid handling robot (Qiagen). cfDNA concentration was determined using Qubit double-strand molecular probes kit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was applied to two tandemly-connected 5 ml Ni-NTA Superflow cartridges (Qiagen); the cartridges were washed with buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Genetics 2021Quote: ... Replicates were lysed in 1 mL of RLT buffer and homogenized using QIAshredder columns (Qiagen, 79654). Total RNA was extracted and eluted in 100 uL RNase-free water using an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL for OD600 ~0.4 for 10% CO2) and stabilized by RNA Protect Bacteria Kit (Qiagen). Next ...
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bioRxiv - Microbiology 2020Quote: ... The proteolysis reaction products were then passed over a 5 mL Ni-NTA superflow cartridge (Qiagen) to remove TEV and uncleaved protein ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...
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bioRxiv - Genomics 2022Quote: ... 300 µL lysis solution (LSDNA, RevoluGen) and 20 µL of 20 mg/mL Proteinase K (Qiagen) was added to the partly-lysed cells and incubated at 56 °C for 20 min ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was applied to a 2-ml column of Ni-NTA-agarose (Qiagen, Hilden, DE) at a flow rate of 1 ml min−1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of 10 mg/mL RNase A (DNeasy Blood &Tissue kit, #69504, Qiagen, Hilden, Germany) was added to each sample and incubated at 37°C for 2 h ...