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Citations for Qiagen :
1401 - 1450 of 1989 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The supernatant was mixed with 1 ml of 50% Ni2+-NTA His-bind resin (Qiagen, Hilden, Germany), incubated for 1 hr at 4°C with mild rotatory shaking ...
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bioRxiv - Microbiology 2021Quote: ... 14 mg/ml) were screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Genomics 2021Quote: DNA was extracted from the donors’ peripheral blood (5 ml) using DNeasy Blood & Tissue Kit from QIAGEN according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Ligated chromatin was de-crosslinked by incubation with 4μL proteinase K (20mg/mL, Qiagen, Cat. 19131, Germany), overnight for 65°C and 300 RPM) ...
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bioRxiv - Microbiology 2021Quote: ... Purification was performed by incubating the cleared lysate with 1 mL Ni-NTA resin (Qiagen, Hilden, Germany) on a rotating platform at 4°C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was supplemented with 10 mM imidazole and incubated with 1 ml Ni-NTA resin (Qiagen) for 1 hour at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell debris was removed by centrifugation and supernatant was mixed with 1 ml Ni-NTA Agarose (Qiagen) and incubated for 90 minutes at 4 °C with gentle shaking ...
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bioRxiv - Systems Biology 2021Quote: ... 2 mL samples were mixed into glass tubes containing 2 volumes of RNAprotect bacteria reagent solution (Qiagen). After mixing and incubating for 5 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was incubated for 2h at 4°C with 5 ml of Ni-NTA agarose (Qiagen) pre-equilibrated in wash buffer 1 WB1 ...
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bioRxiv - Microbiology 2020Quote: ... samples were defrosted and incubated in 1 ml of RNAprotect cell reagent (Qiagen, 5 min, 25°C). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... at which point 1.5 mL of culture samples were collected and resuspended in RNAprotect Bacteria Reagent (Qiagen) and then QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was loaded onto a column containing 1 mL of NiNTA agarose resin (Qiagen, Hilden, Germany) pre-equilibrated with lysis buffer containing 10 mM Imidazole ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was transferred to fresh tubes and incubated with 2 μl RNase A (Qiagen, 20 mg/ml) and 6 μl 5M NaCL (Active Motif ...
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bioRxiv - Genetics 2020Quote: Germline DNA was isolated from 1.5 ml peripheral blood using the QIASymphony DSP DNA Midi kit (QIAGEN), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 mg/mL proteinase K) and DNA clean up (MiniElute reaction clean up kit, 28204, Qiagen). qPCR was performed using mPer2 and Wnt3a primers ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was removed and the cell pellets were resuspended in 1 ml of RNAprotect Bacteria Reagent (Qiagen) and stored -20 until RNA extraction ...
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bioRxiv - Microbiology 2022Quote: ... RNA extraction was performed from 1 mL of the bacterial solution using an RNeasy Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was supplemented with Ni-NTA agarose (Qiagen, 1.3 ml resin/ 1 l insect cell pellet). Subsequently ...
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bioRxiv - Synthetic Biology 2022Quote: ... first the filter-harvested cells were resuspended in 2 mL RNAprotect Bacterial Reagent (Qiagen Catalog no. 76506), then pelleted in a centrifuge ...
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bioRxiv - Plant Biology 2022Quote: ... the clear supernatant was incubated for 4 h with 1.5 ml Ni-NTA agarose (Qiagen, Hilden, Germany) previously washed with 6 ml of distilled H2O and equilibrated with 6 ml of binding buffer (150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... the soluble fraction was loaded onto a Ni-NTA agarose mini-column (0.2 mL) (Qiagen, Hilden, Germany). The column was washed with 10 column volumes of At buffer (50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2022Quote: ... Four wells were then harvested at the appropriate growth stage and combined with 4 mL RNAprotect (Qiagen) to generate each replicate ...
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bioRxiv - Genetics 2022Quote: ... or approximately 30 mg tissue material homogenized in 1 ml TRIzol™ reagent via TissueLyser II (Qiagen) with a 5 mm steel bead for 2 min at 30 Hz ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysate was applied to 4 ml bed volume Ni-NTA Agarose beads (Qiagen, cat. No. 30210) for 1 hour ...
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bioRxiv - Genomics 2022Quote: ... cfDNA was extracted from 2 to 4 ml of plasma using the QIAsymphony liquid handling robot (Qiagen). cfDNA concentration was determined using Qubit double-strand molecular probes kit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from 13 ml of the culture using a DNeasy Blood & Tissue kit (Qiagen) according to the manufacturer’s instructions for Gram-negative bacteria ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from 1.8 ml of culture with a DNeasy ultraclean microbial kit (Qiagen, Germany) according to the manufacturer’s instructions and stored in 10 mM Tris buffer at pH 8 and -80 ⁰C until paired-end reads of 2 x 150 bp were sequenced on a NextSeq 500/550 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA of isolated colony were purified from 7.5 ml of culture (DNeasy Blood and Tissue – Qiagen) with an additional step of cell lysis with microbeads (Precellys Evolution) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris pH 8.5, 2 mM MgCl2, 0.5% NP40, 0.5% Tween20, 0.05 U/mL QIAGEN protease) and following the procedure described in (42) ...
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bioRxiv - Biophysics 2023Quote: ... The filtered supernatant was loaded onto 2 gravity columns each with 4 mL Ni-NTA agarose (Qiagen). The resin was pre-equilibrated with the lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was passed two times over a column with 1.5 mL of Ni2+-NTA resin (Qiagen), washed with 50 bed volumes of wash buffer (20 mM HEPES-KOH ...
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bioRxiv - Microbiology 2023Quote: ... 4 ml of field-saturated peat were added to 2.5 volumes of Lifeguard buffer (Qiagen, Maryland, USA), transferred out of the field on ice in a cooler ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen) in a gravity flow column and then washed with 300 mL of lysis buffer D ...
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bioRxiv - Physiology 2023Quote: ... A small portion of frozen livers (∼20 mg) were homogenized in 1 mL of QIAzol (Qiagen 79306). After the addition of 200 µL of chloroform ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 0.2 ml of each BAL using the QIAamp DNA Blood Mini Kit (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was collected and incubated with 2 mL of Ni-NTA Superflow affinity resin (Qiagen), previously equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... 25 mM NaCl in UltraPure distilled water) with 0.4 mg/ml Proteinase K (QIAGEN N.V., Venlo, NL). Homogenized samples were incubated at 37 °C for 1.5 h followed by 98 °C for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was passed through a 5 mL column volume (CV) of Ni-NTA agarose resin (Qiagen: 30250). The column was then washed with 10 CV of His binding buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant from the second spin was incubated with 3 mL of Ni-NTA agarose beads (Qiagen) for 30 minutes at 4°C in the cold room ...
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bioRxiv - Molecular Biology 2023Quote: Cell-free RNAs (cfRNAs) were extracted from 1 ml of plasma using QIAzol Lysis Reagent (Qiagen, 79306) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Tissue samples of mice were transferred into 2 ml tubes containing 4 mm stainless steel beads (Qiagen) and 1 ml of ice-cold sterile saline ...
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bioRxiv - Genomics 2024Quote: Peripheral Blood samples (2.5 ml) were drawn from each volunteer in a PAXgene tube (Qiagen cat# 762165) followed by total RNA isolation using an RNA isolation kit (Qiagen cat# 763134 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were resuspened in 500 µL 1X PBS and 25 µL RNase A solution (10mg/ml) (Qiagen). Following incubation ...
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bioRxiv - Genomics 2024Quote: Peripheral Blood samples (2.5 ml) were drawn from each volunteer in a PAXgene tube (Qiagen cat# 762165) followed by total RNA (including miRNA ...
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bioRxiv - Genetics 2024Quote: DNA was isolated using 1 ml from whole blood using the QiAamp DNA blood Midi kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The eluates were mixed with guanidine– HCl to a final concentration of 6 M and incubated with Ni-NTA sepharose (Qiagen, 100 μl of slurry per sample) o/n at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from 10 ml cultures of yeast strains and purified with an RNeasy kit (Qiagen). The RNA was prepared for sequencing using the TruSeq Stranded Total RNA Sample Prep adaptor kit (Illumina) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mL of stabilization mix (RNAprotect Bacteria Reagent - Qiagen diluted with PBS in a 2:1 volume ratio) was applied on plates ...
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bioRxiv - Genomics 2020Quote: Nucleic acids were isolated from 4 mL of plasma by using a QIAamp cfDNA/RNA kit (Qiagen 55184) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: Total DNA was extracted from 1 ml of blood sample using DNeasy Blood and Tissue Kit (Qiagen, USA). The concentration of DNA ranged from 2.2 ng/ul to 9.7 ng/ul ...