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Citations for Qiagen :
1351 - 1400 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Supernatant was removed and the cell pellets were resuspended in 1 ml of RNAprotect Bacteria Reagent (Qiagen) and stored -20 until RNA extraction ...
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bioRxiv - Microbiology 2022Quote: ... RNA extraction was performed from 1 mL of the bacterial solution using an RNeasy Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was supplemented with Ni-NTA agarose (Qiagen, 1.3 ml resin/ 1 l insect cell pellet). Subsequently ...
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bioRxiv - Synthetic Biology 2022Quote: ... first the filter-harvested cells were resuspended in 2 mL RNAprotect Bacterial Reagent (Qiagen Catalog no. 76506), then pelleted in a centrifuge ...
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bioRxiv - Plant Biology 2022Quote: ... the clear supernatant was incubated for 4 h with 1.5 ml Ni-NTA agarose (Qiagen, Hilden, Germany) previously washed with 6 ml of distilled H2O and equilibrated with 6 ml of binding buffer (150 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... the soluble fraction was loaded onto a Ni-NTA agarose mini-column (0.2 mL) (Qiagen, Hilden, Germany). The column was washed with 10 column volumes of At buffer (50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2022Quote: ... Four wells were then harvested at the appropriate growth stage and combined with 4 mL RNAprotect (Qiagen) to generate each replicate ...
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bioRxiv - Genetics 2022Quote: ... or approximately 30 mg tissue material homogenized in 1 ml TRIzol™ reagent via TissueLyser II (Qiagen) with a 5 mm steel bead for 2 min at 30 Hz ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysate was applied to 4 ml bed volume Ni-NTA Agarose beads (Qiagen, cat. No. 30210) for 1 hour ...
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bioRxiv - Genomics 2022Quote: ... cfDNA was extracted from 2 to 4 ml of plasma using the QIAsymphony liquid handling robot (Qiagen). cfDNA concentration was determined using Qubit double-strand molecular probes kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris pH 8.5, 2 mM MgCl2, 0.5% NP40, 0.5% Tween20, 0.05 U/mL QIAGEN protease) and following the procedure described in (42) ...
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bioRxiv - Biophysics 2023Quote: ... The filtered supernatant was loaded onto 2 gravity columns each with 4 mL Ni-NTA agarose (Qiagen). The resin was pre-equilibrated with the lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was passed two times over a column with 1.5 mL of Ni2+-NTA resin (Qiagen), washed with 50 bed volumes of wash buffer (20 mM HEPES-KOH ...
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bioRxiv - Microbiology 2023Quote: ... 4 ml of field-saturated peat were added to 2.5 volumes of Lifeguard buffer (Qiagen, Maryland, USA), transferred out of the field on ice in a cooler ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen) in a gravity flow column and then washed with 300 mL of lysis buffer D ...
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bioRxiv - Physiology 2023Quote: ... A small portion of frozen livers (∼20 mg) were homogenized in 1 mL of QIAzol (Qiagen 79306). After the addition of 200 µL of chloroform ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 0.2 ml of each BAL using the QIAamp DNA Blood Mini Kit (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was collected and incubated with 2 mL of Ni-NTA Superflow affinity resin (Qiagen), previously equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... 25 mM NaCl in UltraPure distilled water) with 0.4 mg/ml Proteinase K (QIAGEN N.V., Venlo, NL). Homogenized samples were incubated at 37 °C for 1.5 h followed by 98 °C for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was passed through a 5 mL column volume (CV) of Ni-NTA agarose resin (Qiagen: 30250). The column was then washed with 10 CV of His binding buffer (50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant from the second spin was incubated with 3 mL of Ni-NTA agarose beads (Qiagen) for 30 minutes at 4°C in the cold room ...
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bioRxiv - Biochemistry 2023Quote: ... cells were resuspened in 500 µL 1X PBS and 25 µL RNase A solution (10mg/ml) (Qiagen). Following incubation ...
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bioRxiv - Molecular Biology 2023Quote: Cell-free RNAs (cfRNAs) were extracted from 1 ml of plasma using QIAzol Lysis Reagent (Qiagen, 79306) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Tissue samples of mice were transferred into 2 ml tubes containing 4 mm stainless steel beads (Qiagen) and 1 ml of ice-cold sterile saline ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from 13 ml of the culture using a DNeasy Blood & Tissue kit (Qiagen) according to the manufacturer’s instructions for Gram-negative bacteria ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was extracted from 1.8 ml of culture with a DNeasy ultraclean microbial kit (Qiagen, Germany) according to the manufacturer’s instructions and stored in 10 mM Tris buffer at pH 8 and -80 ⁰C until paired-end reads of 2 x 150 bp were sequenced on a NextSeq 500/550 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA of isolated colony were purified from 7.5 ml of culture (DNeasy Blood and Tissue – Qiagen) with an additional step of cell lysis with microbeads (Precellys Evolution) ...
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bioRxiv - Genomics 2021Quote: ... The eluates were mixed with guanidine– HCl to a final concentration of 6 M and incubated with Ni-NTA sepharose (Qiagen, 100 μl of slurry per sample) o/n at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from 10 ml cultures of yeast strains and purified with an RNeasy kit (Qiagen). The RNA was prepared for sequencing using the TruSeq Stranded Total RNA Sample Prep adaptor kit (Illumina) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mL of stabilization mix (RNAprotect Bacteria Reagent - Qiagen diluted with PBS in a 2:1 volume ratio) was applied on plates ...
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bioRxiv - Genomics 2020Quote: Nucleic acids were isolated from 4 mL of plasma by using a QIAamp cfDNA/RNA kit (Qiagen 55184) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: Total DNA was extracted from 1 ml of blood sample using DNeasy Blood and Tissue Kit (Qiagen, USA). The concentration of DNA ranged from 2.2 ng/ul to 9.7 ng/ul ...
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bioRxiv - Molecular Biology 2019Quote: ... or from 1 mL plasma using QIAamp Circulating Nucleic Acid Kit (QIAGEN, Cat. No.: 55114, QC for short) following the manufacturer’s guide ...
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bioRxiv - Genomics 2019Quote: ... The partially decalcified nodule was then immersed in 1 mL of a digestion buffer with final concentrations of 0.45 M EDTA and 0.25 mg/mL Proteinase K (Qiagen) and rotated at 37°C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... The peak fractions containing Esco2 were pooled and applied onto a 1 ml Ni-NTA Superflow column (Qiagen), equilibrated with 20 mM HEPES pH 7.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... resuspended in 50 mM sodium citrate buffer containing 10 µl of RNase A (20 mg/ml, Qiagen 76254) and incubated for 2 hours at 50°C ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 mL of plasma were thawed and cfDNA extracted using the QIAamp® Circulating Nucleic Acid Kit (Qiagen) according to the manufacturer’s protocol and stored at −20°C until use ...
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bioRxiv - Cell Biology 2019Quote: ... 1 ml of culture was harvested for RNA extraction using an RNeasy® Mini Kit (Qiagen, Hilden, Germany). DNA digestion was done to remove contaminating DNA in the solution before RNA clean up ...
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bioRxiv - Genetics 2020Quote: ... plasmids were isolated from 200 mL of these cultures using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965). The remaining 200 mL of the overnight cultures were spun down ...
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bioRxiv - Systems Biology 2020Quote: Three milliliters of cells from mid-log phase cultures were mixed with 6 mL RNAprotect Bacteria Reagent (Qiagen). Samples were mixed immediately by vortexing for 5 s ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was applied to a 0.1 ml column of Ni2+-NTA Superflow resin (Qiagen Cat No. 30410) and protein was purified using the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... total cfDNA was extracted from 3 mL of separated plasma using the QIAamp Circulating Nucleic Acid kit (QIAGEN) following manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... 20 mM imidazole was added to the supernatant and incubated with 5 mL of Ni-NTA resin (Qiagen) with a stirring magnet at 4°C for 30 min ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... The soluble fraction was split between two columns each with 2 ml Ni-NTA agarose beads (Qiagen, 30210) pre-equilibrated with Lysis Buffer ...
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bioRxiv - Biophysics 2022Quote: ... The suspension was spun down at 16,000 r.c.f at 4 °C for 30 min and the supernatant was applied 5 ml Ni-NTA resin (Qiagen)/ L culture medium ...
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bioRxiv - Developmental Biology 2022Quote: ... The concentrated SPACA6-containing media was applied onto a 2-mL column of Ni-NTA agarose resin (Qiagen). The Ni-NTA resin was washed with 10 column volumes (CV ...
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bioRxiv - Immunology 2022Quote: ... The upper aqueous phase containing RNA was carefully transferred to a 2 ml collection tube (cat. 990381, Qiagen) without touching the interphase and placed in a QIAcube instrument for extraction.RNA extraction was carried out using the recommended protocol (FIW-50-001-J_FW_MB and PLC program version FIW-50-002-G_PLC_MB ...
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bioRxiv - Microbiology 2021Quote: ... the cell lysate was loaded by gravity flow onto a column containing 1 ml Ni-NTA resin (Qiagen) equilibrated with buffer A ...
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bioRxiv - Developmental Biology 2022Quote: ... SPACA6 concentrated to 7 mg mL−1 in Buffer B was mixed in a 1:1 volumetric ratio (0.3:0.3 mL) with JCSG+ (Qiagen), Cryos (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet, Qiagen) and incubated for 30 mins at 4 °C on a rotating wheel ...