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Citations for Qiagen :
1051 - 1100 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Dehydrated samples were stored at −80 °C until resuspension in 25 μl or 100 μl of Buffer EB (QIAGEN, Venlo, Netherlands).
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bioRxiv - Plant Biology 2020Quote: ... For expression analysis total RNA was extracted from 100 mg of freeze-dried leaf tissue of chilli and tomato lines using RNeasy plant kit (Qiagen, USA) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Frozen muscle was pulverized in liquid nitrogen and genomic DNA was extracted using a Genomic Tip 100/G kit (Qiagen, USA). DNA quantity was measured using a NanoDrop ND-8000 spectrophotometer and a Qubit dsDNA BR Assay kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl of each input and biotinylated RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen #74104) using the modified protocol ...
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bioRxiv - Immunology 2022Quote: ... DNA synthesis reaction was carried out in 100 μl using 10 pmol of each primer with HotStarTaq Plus DNA Polymerase (Qiagen, 203603). Purified dsDNA products were amplified with 10 pmol of each primer in a 100 μl total reaction volume ...
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bioRxiv - Genetics 2022Quote: ... Size selection was also used for purification of 100 bp-200 bp PCR products by QIAquick Gel Extracton (28706, QIAGEN, Germany).
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bioRxiv - Genomics 2022Quote: ... Individual nuclei were sorted on a Sony Sorter SH800 with a 100 μm sorting chip into wells of a 96-well plate containing 5 μl of Buffer TCL (Qiagen #1031576) with 1% β-mercaptoethanol (ThermoFisher #21985023).
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bioRxiv - Genetics 2022Quote: ... The aqueous phase was mixed with an equal volume of 100% ethanol and transferred to an RNeasy MinElute spin column (Qiagen, 74104) for purification according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was rinsed with 70% ethanol and dried at room temperature before dissolving in 100 μL of RNase-free DNase set (Qiagen 79254) and incubated at room temperature for 10 minutes to remove DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... total RNA was extracted from a pool of 100–120 adult females per line using the RNeasy plus mini kit (Qiagen, Belgium) with 4 replicates for strains MAR-ABi and JP-RRi and 5 replicates for strains ROS-ITi and SOL-BEi ...
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bioRxiv - Molecular Biology 2023Quote: ... the other half of tumor tissues were lysed using lysis buffer (0.1% Triton X-100) and a TissueLyser homogenizer (Qiagen, Hilden, Germany). DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... Bisulfite conversion was performed on 100 ng of genomic DNA using the EpiTect Fast DNA Bisulfite kit from Qiagen (Cat.# 59824) and DNA libraries were constructed using the NUGEN Ovation RRBS Methyl-seq System (Cat.# 0353 ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was performed from 200 mg of three independent samples using the MO BIO’s Powersoil DNA isolation kit procedure (Qiagen 12888-100) as described previously (von Gastrow et al. ...
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bioRxiv - Genomics 2023Quote: ... The sample was then centrifuged at 15000 rpm for 10 min and the pellet was resuspended in 100 μl buffer EB (Qiagen, 19086). For each sample of a 96-well plate ...
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bioRxiv - Bioengineering 2023Quote: ... samples were suspended in RNeasy kit Lysis Buffer and β-mercaptoethanol (1:100 v/v) and homogenized using QIAshredder columns (Qiagen). Samples loaded on spin columns were treated with DNase (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial genomic DNA was isolated from pure culture using QIAGEN Genomic-tip 100/G following the manufacturer’s instruction of (Qiagen, Valencia, CA). The Seqwell plexWell LP384 Library Preparation Kit and Native Barcoding Kit 24 V14 (SQK-NBD112.24 ...
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bioRxiv - Cell Biology 2023Quote: ... all samples were diluted to the same RNA concentration and 250 ng of RNA from each sample spiked with 100 ng mouse RNA to control reaction efficiency were reverse transcribed using the miScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: MiR libraries were generated from 100 -150 ng of cell using the QIAseq miRNA Library Kit according to the protocol (Qiagen, Germany). Library quality control and concentrations were determined using a High Sensitivity D1000 ScreenTape with TapeStation Analysis Software A.02.02 (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... ∼200 picked cells were stored in RLT buffer (Cat. No. 79216 – 220 mL, Qiagen) with 1% 2-Mercaptoethanol (M6250-100ML ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 mM of NaCl and 2 μL of RNase A (0.5 mg / mL) (Qiagen) were added to the collective eluates which were incubated at 65 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... Periplasmic fraction was incubated with 1 mL pre-washed Ni-NTA agarose (30230, Qiagen) for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... the supernatant was applied to 2 mL of pre-washed Ni-NTA resin (Qiagen) at 4°C ...
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bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 1 ml of the slurry using a commercial kit (Qiagen).
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then treated with RNase A (2 mg/ml in water, Qiagen, 19101) for 2 hours at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was loaded onto a 5 ml Ni-NTA agarose bead slurry (Qiagen) equilibrated with equilibration buffer [20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biophysics 2021Quote: The supernatant was mixed with 2 mL Ni-NTA resin (Ni-NTA agarose; Qiagen) pre-equilibrated with buffer A200 (50 mM Tris pH 7.6 ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were passed over 5 ml of packed Ni-NTA agarose resin (Qiagen) at 1.0 ml/min ...
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bioRxiv - Cell Biology 2021Quote: ... before adding it to 2 ml of Strep-Tactin superflow resin (Qiagen, Hilden, Germany) which was pre-equilibrated with 10 ml lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D532 was purified using 1 mL Ni-NTA agarose resin (Qiagen) according to the manufacturer’s instructions and as described previously [27] with slight modification ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were sorted into 1.5 ml buffer RLT from RNeasy Plant Mini-Kit (Qiagen) containing 1% v/v β-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was supplemented with 1 mL pre-equilibrated Ni-NTA resin (Qiagen) and 10 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was added to 1 mL of washed Ni-NTA bead slurry (Qiagen) and incubated for 1 hour at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... three cocoa beans were homogenised in 30 ml of RNAprotect (Qiagen, Venio, The Netherlands) in a 50 ml falcon and vortexed for 30 seconds to disrupt the mucilaginous pulp from the seeds ...
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bioRxiv - Microbiology 2021Quote: ... The resulting supernatant was supplemented with 0.75 mL pre-equilibrated Ni-NTA resin (Qiagen) and 20 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was gently shaken with 0.5 ml of Ni-NTA agarose suspension (Qiagen) for 1 h at 4 °C and transferred to a filter column where the liquid was drained ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was added to 0.5 mL (bed volume) Ni-NTA resin (Qiagen) equilibrated in wash/lysis buffer ...
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bioRxiv - Genomics 2022Quote: ... DNA was further treated with 50 µg/ml of RNase enzyme (Qiagen, Hilden, Germany) for 1 h followed by phenol/chloroform extraction ...
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bioRxiv - Microbiology 2022Quote: ... 200 mL of the pooled library were purified using the MinElute purification kit (Qiagen) and final elution of the BarSeq library was performed in 30 mL in DNAse and RNAse free water.
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bioRxiv - Cancer Biology 2022Quote: ... with 10 μL proteinase K (50 μg/ml, Qiagen, DNeasy Blood & Tissue Kit, #69504) was then added to each tube and incubated with the tail overnight at 60°C ...
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bioRxiv - Microbiology 2019Quote: ... The Ni-NTA matrix was loaded on a prepared 1 ml Polypropylene column (Qiagen) and a filtered P ...
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bioRxiv - Microbiology 2020Quote: ... the media was collected and applied to 3 ml of Ni-NTA resin (Qiagen). The resin was collected and washed with PBS supplemented with 20 mM imidazole ...
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bioRxiv - Cancer Biology 2019Quote: ... Good-quality cDNA was cherry-picked and normalized to 2µg/ml with EB (Qiagen) on the Biomek FxP Workstation into a new 384-well plate (4titude).
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bioRxiv - Molecular Biology 2021Quote: ... These pooled fractions were applied to 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer B with 10 mM imidazole ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and stored in either 1 ml Cell Lysis Solution (Gentra Puregene Kit, Qiagen, USA), Queen’s buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was applied to two 5 ml Ni-NTA Superflow cartridges (Qiagen). The columns were washed with 20 mM HEPES pH 8.0 ...
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bioRxiv - Genomics 2020Quote: ... Next, >300mAU (500μL of >600 mAU/ml, solution) Proteinase K (Qiagen Cat No. 19131) was added to the sample and incubated at 55° C for 3 hours ...
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bioRxiv - Microbiology 2021Quote: ... The soluble extracts were applied to a 1 ml of Ni2+- NTA-agarose (Qiagen) column that had been equilibrated with buffer A containing 10 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble fraction was incubated with Ni-NTA agarose beads (0.5 ml; Qiagen) for 3 hrs ...