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Citations for Qiagen :
851 - 900 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended with 1 mL of QIAzol (QIAGEN Inc.; Germantown, MD) and transferred to tissue homogenizing CKMix tubes 2mL (Bertin Instruments ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then applied to a 3 mL Ni-NTA (Qiagen) column ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by affinity chromatography using a 5 mL Ni-NTA (Qiagen) column ...
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bioRxiv - Biophysics 2022Quote: ... the supernatant was incubated overnight with 3 ml Ni-NTA beads (Qiagen) preequilibrated with the extraction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The column was prepared with 2 mL Ni-NTA Agarose Resin (Qiagen). The resin was equilibrated with 10 column volumes of column buffer and resuspended with the supernatant cell extract for 1 h at 4°C with orbital shaking ...
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bioRxiv - Physiology 2023Quote: ... Agarose beads were collected in 1 ml propylene gravity flow columns (Qiagen) and washed with 10 ml Resuspension buffer ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were re-dissolved in TE + 10 μg/ml RNAse (Qiagen Corp.).
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 200 µg mL-1 RNaseA (Qiagen, 19101), 50 µg mL-1 propidium iodide (Sigma Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cells were harvested in 1 mL QIAzol Lysis Reagent (Qiagen, Hilden, Germany) for 5 min at RT and 200 µL chloroform (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 1.5 mL Ni-NTA agarose resin (Qiagen) on a roller at 4 °C for 1.5 hour ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 1 mL of Ni-NTA agarose (Qiagen) pre-equilibrated with lysis buffer via nutation at 4 °C for 1 hr ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then incubated with 10 mL Ni-NTA beads (QIAGEN) for 90 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... We added 0.7 mL ETR buffer (From QIAGEN Plasmid Plus Midi Kit) to wash the column ...
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bioRxiv - Genetics 2023Quote: ... The supernatant was incubated with 5 ml of Ni- NTA resin (Qiagen) for 2h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... These tissue pools were directly immersed in 1 ml QIAzol (Qiagen, Germany) and stored at -80°C until further processing ...
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bioRxiv - Microbiology 2024Quote: ... or PAXgene fixative (PFPE, PAXgene Tissue FIX Container; 50 ml; Qiagen, 765312), for 16 hours and subsequently embedded in paraffin wax ...
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bioRxiv - Biochemistry 2023Quote: ... and immediately supplemented with 10 mL pre-washed Ni2+-NTA agarose (QIAGEN) followed by incubation for 2 h at 4 °C with rotation ...
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bioRxiv - Microbiology 2024Quote: ... The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen) on a rotator for 1 hour at 4°C ...
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bioRxiv - Genetics 2021Quote: ... were processed for DNA extraction using the Genomic-tip 20/G (mini prep, starting with approx. 108 cells) or 100/G (midi prep, 109 cells) kit (QIAGEN), according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... The supernatant was then used for high molecular weight (HMW) genomic DNA extraction according to the Genomic-tips protocol (100/G, Blood & Cell Culture DNA Midi Kit, QIAGEN).
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples of 50 milligrams of asexual KG females (approximately 120 individuals) were used for DNA extractions with Genomic-tip 100/G (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from cavin1a/1b DKO and WT zebrafish embryos (> 100 embryos randomly selected from 1 clutch) using the RNeasy Mini Kit (QIAGEN) and cDNA synthesis was performed using SuperscriptIII reverse transcriptase (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the best diffracting crystal for the 14-3-3σ Clu3/pS197 complex grew in condition G2 (0.2 M NaBr, 100 mM BisTris propane pH 7.5, 20% PEG 3350) of PACT screen (Qiagen, Hilden, Germany). Crystals were mounted in nylon loops directly from crystallization drops and flash frozen in liquid nitrogen without additional cryoprotection ...
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bioRxiv - Molecular Biology 2020Quote: ... and then the samples were incubated with 100 μl of pre-cleared 50% slurry of Ni-NTA resin (Qiagen, 30210) for 2–3 h at room temperature on a rotating shaker ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse tissue disruption and homogenization was performed in RIPA buffer supplemented with 1:100 protease using Tissue Lyser LT (Qiagen), for 5 min at 50 Hz ...
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bioRxiv - Genomics 2020Quote: ... The aqueous phase was removed and mixed with ½ volume of 100% ethanol before adding to a column of the RNeasy kit (Qiagen). After addition to the column ...
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bioRxiv - Genomics 2020Quote: ... following the manufacturers’ instructions with the only change being a 10 minute incubation at 37 degrees prior to the final elution spin and eluted in 100 μl of EB buffer (QIAGEN). Samples were stored at −20 C.
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bioRxiv - Molecular Biology 2019Quote: ... 50 μl each input and purified RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen) using the modified protocol as described (Duffy and Simon ...
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bioRxiv - Microbiology 2019Quote: ... A second round of amplification was performed by transferring 5µl of 1:100 diluted Round 1 product into 15µl Fast Cycling PCR Mix (QIAGEN, Hilden, Germany) reactions ...
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bioRxiv - Genomics 2020Quote: High-molecular-weight DNA from white (20025) and yellow fat (70346) individuals was extracted from liver tissue using Genomic-tips (G/100) from Qiagen, and fragments smaller than 25Kb were progressively depleted using a Short Read Eliminator kit (Circulomics ...
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bioRxiv - Genetics 2019Quote: ... We extracted total RNA from 100 mg of flower buds right before flowering of all the parental accessions using RNeasy Plant Mini Kit (QIAGEN) with RNase-free DNase I (Invitrogen) ...
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bioRxiv - Genomics 2019Quote: ... We used a 100 µm nozzle to sort single cells into 96-well plates containing 5 µl TCL buffer (Qiagen) with 1% beta-mercaptoethanol for Smart-seq2 and 384-well plates containing 0.6 µl 1% NP40 (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... and 100 mg of ground leaves were used for DNA extraction using the DNeasy® Plant Mini Kit (Qiagen, Valencia) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2019Quote: ... Total environmental DNA was isolated from this 100 μL cell suspension by the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany), following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2021Quote: ... The his-tagged 3C protease was removed by flowing and washing off cleaved transporter protein over two 100 µl beds of Ni-NTA Superflow resin in tandem (Qiagen). Eluent was concentrated using 100 kDa cut-off Amicon Ultra filters (Millipore-Sigma).
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bioRxiv - Bioengineering 2021Quote: ... Exosome samples were treated with 1 µL RNAse A/T1 per 100 µL sample for 30 min at 37°C and lysed in 650 µL of QIAzol (Qiagen). To monitor the RNA recovery percentage as a function of the isolation procedure ...
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bioRxiv - Microbiology 2021Quote: ... 3-20 μl of individual PCR products (adjusted based on estimated relative amplicon concentration) were combined into 100 μl sub-pools and purified using an UltraClean 96 PCR Cleanup Kit (Qiagen). Blank extractions ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from ∼100 mg of cecal contents using the MoBio UltraClean Soil DNA extraction kit (Qiagen, Carlsbad, CA) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y cells were treated either with 100 μM cycloheximide (CHX) or DMSO for 6 hours (41) before harvesting the RNA through RNeasy Minikit (Qiagen). Reverse transcription was performed using RevertAid cDNA synthesis kit (Thermo) ...
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bioRxiv - Plant Biology 2021Quote: RNA was extracted from ∼100 mg of root tissue and DNase treated using the RNeasy plant mini kit and RNase free DNase kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNAs were prepared using 100 mg of homogenized tissues and RNeasy Plant Mini kit with on-column DNase treatment (Qiagen). Libraries for RNA-Seq were prepared from 1 µg of total RNAs with the TruSeq Stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Cell Biology 2021Quote: Transgenic parasites were generated by electroporation of sorbitol-synchronized ring-stage parasites with 50-100 μg of purified plasmid DNA (Qiagen). To select for the plasmids pArl-PfCentrin1-GFP and p3-NLS-L3-mCherry ...
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bioRxiv - Neuroscience 2019Quote: ... Library fragments in the range of 100-800 bp were size-selected using agarose gel electrophoresis followed by DNA gel extraction (QIAGEN). Recovered DNA was further cleaned and concentrated using a column (Zymo Research) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates (2 × 104 cells/well) and reverse-transfected with 100 ng/well of Cignal ISRE-Luc reporter (Qiagen) using Lipofectamine 3000 ...
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bioRxiv - Microbiology 2021Quote: ... Individual blood-fed female flies were separated into Eppendorf tubes containing 100 uL of DNA lysis buffer (Gentra Puregene Tissue Kit, Qiagen) and stored at -20°C.
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bioRxiv - Molecular Biology 2022Quote: ... Biotin-labelled RNA was eluted with 100 μl elution buffer (100 mM DTT in nuclease-free water) and cleaned up with the RNeasy MinElute Cleanup kit (QIAGEN), adjusting the amount of ethanol to capture RNA < 200 nucleotides in length ...
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bioRxiv - Plant Biology 2019Quote: ... total RNA was extracted from 100 mg of apical parts of primary inflorescences of exo70a2 and WT plants using the RNeasy kit (Qiagen). Prior to reverse transcription ...
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bioRxiv - Microbiology 2019Quote: ... Undigested debris were spun down 3 min 10000 rpm and the supernatant was supplemented with 500 μl of 100% ethanol prior loading onto RNeasy mini columns (Qiagen). From this step onwards ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were washed once with 0.1% PBSA and incubated with 50 μL of a 1:100 dilution of anti-penta-His Alexa 647 antibody (Qiagen) for 10 minutes on ice ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was isolated from approximately 100 EBs (differentiation day 2 and 3) or 25 EBs (differentiation day 4 and 5) using RNeasy Mini kit (Qiagen). 0.5 μg RNA was transcribed to cDNA using QuantiTect Reverse Transcription kit (Qiagen) ...