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Citations for Qiagen :
901 - 950 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The ethanol was then poured out and the pellet was air-dried for 15 minutes before resuspension in 50 to 100 μL of DNA Hydration Solution (Qiagen). DNA was sequenced with paired end Illumina sequencing at 30x coverage.
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bioRxiv - Evolutionary Biology 2021Quote: ... Most DNA samples for ONT sequencing were extracted from 100 female flies from each strain using the Blood and Cell Culture DNA Mini Kit (Qiagen) following manufacturer’s instructions with small modifications (Table S2 ...
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bioRxiv - Genetics 2021Quote: High-quality genomic DNA of yeast for sequencing was extracted using the QIAGEN Blood & Cell Culture Kit with 100/G or 20/G Genomic-tips (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... These plasmids were purified and DNA extracted using QIAGEN Plasmid Midi Kit (100) (cat. n° 12145, QIAGEN, Valencia, CA, USA), according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was isolated from 2 clones of each strain corresponding to 50 OD600 units with the Genomic DNA Buffer Set and Genomic-tip 100/G (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1.5X volumes of 100% ethanol was used to precipitate RNA prior to column loading and RW1 buffer was substituted with RWT (Qiagen). For cDNA synthesis of mRNAs ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl each input and purified RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen) using the modified protocol as described (Duffy and Simon ...
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bioRxiv - Genomics 2021Quote: Strains were grown overnight in 50 ml YPD broth and genomic DNA was extracted from approximately 4×109 cells using a QIAGEN Genomic-tip 100/G kit (10223, QIAGEN) with minor modifications ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 100 µl of harvest of culture-enriched samples using DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany) as previously described [22] ...
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bioRxiv - Immunology 2020Quote: ... cleaning were used to purify 100 μl of pooled cDNA with final elution in 15 μl PCR-grade H2O (Qiagen). After quantification with Qubit dsDNA HS assay (Thermofisher) ...
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bioRxiv - Genomics 2022Quote: ... the genomic DNA of the Nichi01 strain was isolated from the silk glands of two male fifth-instar larvae using Genomic-tips 100/g and Genomic DNA Buffer Set (QIAGEN). Long-read sequencing for genome assembly was performed using continuous long-read (CLR ...
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bioRxiv - Genomics 2022Quote: ... The mixture was then centrifuged for 20 min at 12 000 x g and the clarified lysate transferred to an appropriate buffer QBT-equilibrated QIAGEN Genomic-tip 100/G column (Qiagen), after which the column was washed three times with 7 ml Buffer QC and HMW DNA was eluted with 5 ml Buffer QF ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA for MLPA and ddPCR assays was extracted from 100 mg leaves with a DNeasy Plant Mini Kit (Qiagen), according to manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Total RNA was isolated from 50 to 100 μl of packed worms from wild type and brd-1(null) using the RNeasy Mini Kit (74104; Qiagen) and QIAshredder (79654 ...
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bioRxiv - Plant Biology 2022Quote: ... solutions for 0- and 30-minutes Total RNA was extracted from 100 mg of frozen and ground tissue from mock and treated seedlings using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s protocol (31) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were induced with 100 μM IPTG at 12°C for 16 hrs and purified on a Ni2+ column according to manufacturer protocols (Qiagen). Proteins were eluted using 125 mM imidazole ...
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bioRxiv - Neuroscience 2022Quote: ... Tubes were spun for 15 minutes and the RNA in the aqueous phase was precipitated with 100% ethanol and transferred into an RNeasy column (Qiagen). 30μl of RNA per sample were collected and the concentration and purity were determined using a Nanodrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... bead beating was performed on 100 mg of cecal contents for 10 min using a tissue lyser (Qiagen, Germantown, MD). Remaining steps for DNA isolation were performed as per manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA (gDNA) was extracted from stationary broth culture using genomic DNA-Tip 100/G system and buffer set (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 100,000 cells were transferred to a tube containing 100 µl of RLT buffer (Qiagen, Hilden, Germany). Cells were stored at –80 °C until further processing ...
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bioRxiv - Cell Biology 2023Quote: ... into parasites already ectopically expressing NLS-mCherry (previously provided by Markus Ganter (Simon et al., 2021a)) with 50–100 μg of purified plasmid DNA (QIAGEN). Plasmid precipitation was done prior to transfection as stated above ...
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bioRxiv - Microbiology 2023Quote: A total of 100 ng total RNA was used for library preparation via the QIAseq miRNA Library Kit (#331502, Qiagen) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified cDNA was subsequently isolated by separation in a 3% agarose gel and isolation of cDNAs 200 to 600 base pairs in length (corresponding to 100 to 500 nucleotide RNAs plus adaptors) using QIAquick gel extraction kit (Qiagen) according to manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... 50 μl each input and purified RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen) using the modified protocol as described (Duffy and Simon ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 350□μl of 100% ethanol was added to the sample and loaded onto a RNeasy MinElute column (QIAGEN). RNA was isolated using RNeasy Mini kit (#74104 ...
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bioRxiv - Cancer Biology 2022Quote: ... Short-fragment size-selection was performed by cutting out gel bands between 100-200 bp and subsequent purification using the QiaQuick gel extraction kit (Qiagen) according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the lysate was spun out of the Feather cartridge at 4000x g and added 100 µL of Ni-NTA agarose resin (Qiagen) to capture HIS-tagged proteins ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA was extracted from approximately 100 mg of the plant tissues using a DNA isolation kit (PowerSoil, Qiagen, USA) following the manufacturer’s instructions ...
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Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomatobioRxiv - Plant Biology 2023Quote: ... DNA was extracted from 100 mg of ground frozen leaf and fruit tissue using Qiagen DNeasy Plant Mini Kit (Qiagen) following the manufacturer’s instructions and resuspended in 30 μL H2O ...
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bioRxiv - Microbiology 2023Quote: ... equal amounts (200 µl) of FruK and Cra cell lysates were mixed with 100 µl of nickel-nitrilotriacetic acid beads (Qiagen). After overnight incubation at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 100 to 150 mg of powder was used to extract total RNA using the RNeasy Plant Mini Kit from QIAGEN. On-column DNase treatment was performed using the RNase-free DNase kit from Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... the upper aqueous phase containing RNA was mixed with equal amounts of 100% ethanol and was then loaded onto an RNA spin column (Qiagen) and centrifuged for 30 seconds at 8000 rpm ...
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bioRxiv - Physiology 2023Quote: DNA was extracted from fecal pellets (100 mg, n=5 per group) by the QIAmp Power Fecal DNA kit (12850-50, Qiagen). The V4 region of 16S rRNA gene was amplified ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the bacterial strains grown in VL55 medium with 5 mM pyruvate using the Qiagen Genomic-tip 100/G DNA isolation kit and associated DNA buffers (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription of 100 ng total RNA from each sample to cDNA was accomplished with the QuantiTect Reverse Transcription Kit (Qiagen) at 42°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were incubated with a PCR mix containing (100 µM dNTP, 1X thermopol Taq reaction buffer, 1X of Q solution (Qiagen) and 1 µM of each primer) ...
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bioRxiv - Immunology 2024Quote: ... The upper aqueous layer was mixed with an equal volume of 100% ethanol and processed using miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Genomics 2024Quote: ... The bacterial cells were harvested by centrifugation and the DNA extraction was performed by the used of Genomic-tip 100/G column (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Fecal pellets were collected and stored at −80°C until DNA extraction with DNeasy PowerLyzer PowerSoil Kit (Qiagen; 12855-100). Fecal Rg abundance was determined with Rg-specific 16S rDNA primers ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from approximately 100 mg of frozen samples according to a modified RNeasy Plant Mini Kit (Qiagen, Germany) procedure [21] ...
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bioRxiv - Immunology 2024Quote: ... 100-200 μm thick sections were cut and used to extract total RNA using the RNeasy Mini Kit (Qiagen, USA). The RNA integrity number (RIN ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted in random order from the faecal slurries (n=484) using DNeasy PowerLyzer PowerSoil kit (Qiagen, 12855-100) and the V3-region of the 16S rRNA gene was PCR amplified using 0.2 µl Phusion High-Fidelity DNA polymerase (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... alphenor females that were homozygous for the h or H alleles using the QIAgen GenomicTip G-100 kit (QIAgen, USA). Extractions followed the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... samples were resuspended in 100 μl TE buffer pH 8 and RNA extraction performed according to the RNeasy Mini Kit (Qiagen) protocol with few modifications ...
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bioRxiv - Neuroscience 2024Quote: ... DRG and paw skin RNA was extracted using a RLT buffer:2-mercapto ethanol mixture in a 100:1 ratio/RNeasy (Qiagen) RNA mini kit according to the manufacturer’s instructions and spinal cord and spleen RNA was extracted using TRizol (Invitrogen)/RNeasy RNA mini kit ...
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bioRxiv - Genomics 2024Quote: ... Digested product was then run on a 4% agarose gel with 1x SYBR Safe for 45 minutes at 100 V and gel extracted (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... falciparum 3D7 ring stage parasites were transfected with 100 μg of purified plasmid DNA (Plasmid Maxi Kit, Qiagen, Valencia, CA) by electroporation (310 V ...
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bioRxiv - Molecular Biology 2020Quote: ... and clarified lysate was incubated with 1 ml packed Ni-NTA resin (QIAGEN) per 750 ml culture for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated NiNTA resin (Qiagen). The column was washed extensively with Nickel buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Synthetic Biology 2019Quote: ... where it was incubated with 2 mL] Ni-NTA agarose bead slurry (QIAGEN) for 10 min at 4 °C ...