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Citations for Qiagen :
951 - 1000 of 6183 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... or the RNeasy Mini kit RNase-Free DNase set (QIAGEN) in triplicate according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... with an additional DNAse treatment (Qiagen RNase-Free DNase Set). RNA concentration ...
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bioRxiv - Microbiology 2022Quote: ... RNA was treated with DNase (DNase-Free DNase Set, Qiagen) and repurified using the miRNeasy micro plus Kit (Qiagen) ...
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bioRxiv - Physiology 2022Quote: ... combined with on-column DNase digestion (DNase Set, 79254, Qiagen) as described in the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Residual DNA was removed using RNAse-Free DNase Set (Qiagen). RNA was assessed for quality and quantity (TapeStation ...
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bioRxiv - Plant Biology 2024Quote: ... and treated with the RNase-Free DNase Set (Qiagen, Germany). 1 μg total RNA was reverse transcribed with oligo dT using Sensifast cDNA Synthesis kit (Meridian Bioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was treated with DNase (DNase-Free DNase Set, Qiagen) and repurified using the RNeasy micro plus Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was depleted with the RNase-free DNase Set (Qiagen). The quality of purified RNA was tested on an Aligent 2200 Tapestation using RNA screentape ...
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bioRxiv - Bioengineering 2024Quote: ... including DNase treatment with RNase-Free DNase Set (Qiagen, #79254). At the final step ...
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bioRxiv - Immunology 2023Quote: ... and additionally purified using an RNase Free DNase Set (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). Sequencing libraries were constructed following steps from the manufacturer’s instructions (VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina® ...
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bioRxiv - Cancer Biology 2023Quote: ... and on-column DNase digestion (RNase-free DNase Set, Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... with on column DNase digest (RNase-Free DNase Set, QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and RNase-Free DNase Set (cat# 79254, QIAGEN, GmBH, Germany). cDNA libraries were constructed following Illumina standard protocols and sequenced with an Illumina NovaSeq 6000 by SHBIO (Shanghai ...
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bioRxiv - Neuroscience 2023Quote: ... treated with DNase (RNase-Free DNase Set, QIAGEN; Milan, Italy) and eluted in RNase/DNase-free water ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was depleted using the RNase-Free DNase Set (Qiagen). In the final step ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was treated with RNase-free DNase set (QIAGEN). RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s protocols (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... mRNA was on-column DNase treated with DNAse Set (Qiagen). Library preparation was performed by Novogene ...
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bioRxiv - Plant Biology 2024Quote: ... mRNA was on-column DNase treated with DNAse Set (Qiagen). mRNA concentration was quantified using a NanoDrop spectrophotometer and 1 μg of cDNA was synthesised using the qScript cDNA Supermix kit ...
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bioRxiv - Plant Biology 2024Quote: ... mRNA was on-column DNase treated with DNAse Set (Qiagen). Library preparation was performed by Glasgow Polyomics using the Illumina TruSeq mRNA kit (polyA selection) ...
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bioRxiv - Neuroscience 2024Quote: ... Contaminating DNA was removed using RNase-Free DNase Set (Qiagen). The RNA was reverse transcribed using RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... 200 ng of RNA was used in the RT reaction with the miScript RT kit (Qiagen). qPCR was performed using a custom LNA probe for miR-1002 using the miRCury SYBR green reagent (Qiagen).
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bioRxiv - Neuroscience 2024Quote: The RT reaction was performed using the QIAGEN miRCURY LNA RT Kit (Cat. No.: 339340, QIAGEN). The reaction mix included 2 µl of 5x miRCURY SYBR® Green RT Reaction Buffer ...
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bioRxiv - Microbiology 2022Quote: ... carried out qRT-PCR reactions with gene-specific SYBR green-based primer assays (either QuantiTect assays from Qiagen or PrimeTime assays from IDT, Inc.) and PowerUp SYBR green master mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... or miScript II RT Kit (Qiagen). Noteworthy ...
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bioRxiv - Developmental Biology 2021Quote: ... or miScript II RT kit (Qiagen). The qRT-PCR was carried out using Light Cycler 480 SYBR Green I Master (Roche) ...
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bioRxiv - Immunology 2021Quote: ... or the Sensiscript RT kit (Qiagen). Quantitative real-time PCR was performed using the SsoAdvanced- Universal SYBR Green Supermix (Biorad) ...
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bioRxiv - Bioengineering 2021Quote: The miScript II RT kit (Qiagen) was used according to manufacturer’s protocol for cDNA synthesis from 1 μg of RNA ...
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bioRxiv - Microbiology 2021Quote: ... 1x OneStep Ahead RT mix (Qiagen), 0.5 µM each primer ...
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bioRxiv - Physiology 2022Quote: ... miScript II RT Kit (Qiagen, UK) was used and cDNA synthesis was performed according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... and the Omniscript RT kit (Qiagen). Specific primers were designed from O ...
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bioRxiv - Cancer Biology 2022Quote: ... miRCURY LNA RT Kit (Qiagen, 339340) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... Using miRCURY LNA RT kit (Qiagen), we first generated cDNA ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA produced (QuantiTech RT, Qiagen). Primers for Jamaican fruit bat genes (Supplemental Table 2 ...
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bioRxiv - Immunology 2023Quote: ... and the Omniscript RT Kit (Qiagen). The cDNA was amplified by PCR using KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Bioengineering 2023Quote: ... via the OmniScript RT Kit (Qiagen) according to the manufacturer’s instructions using RNase Out (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... In situ hybridization for mature miR-18a was performed using a miRCURY LNA detection probe (Exiqon/Qiagen, Germantown, MD), labeled with DIG at the 5′ and 3′ends ...
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bioRxiv - Developmental Biology 2022Quote: ... we treated rat AFSCs at 60% confluence with miR-17-5p and - 20a power inhibitors (25 μM) following the manufacturer’s protocol (Qiagen YI04100215-DDA ...
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bioRxiv - Developmental Biology 2020Quote: Hsa-miR mimics (339173, miRCURY LNA miRNA Mimic) and inhibitors (339121, miRCURY LNA miRNA Inhibitors) were ordered from Qiagen, Hilden ...
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bioRxiv - Developmental Biology 2019Quote: ... The TSB (5’-ATTATTGCACCCAGTGCC-3’: the miR-92a-3p target site is underlined) was designed and produced by Qiagen, with an additional scrambled TSB to act as a negative control (5’-TAACACGTGTATACGCCCA-3’) ...
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bioRxiv - Molecular Biology 2021Quote: Potential biological connections of all DE miRs were identified using Ingenuity Pathway Analysis (IPA) (IPA, Qiagen Redwood City, USA) ‘Core Analysis’ ...
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bioRxiv - Genomics 2022Quote: ... 25 fmol of the exogenous synthetic spike-in control Caenorhabditis elegans miRNA cel-miR-39 (Qiagen, Cat. No. 219610) was spiked into samples at the beginning of the extraction procedure to check both the extraction of miRNAs and the efficiency of the cDNA synthesis ...
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bioRxiv - Cancer Biology 2022Quote: ... after adding 1.0×10^6 copies/µL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and hsa-miR-519a-3p miRCURY LNA miRNA Mimic or its related Negative Control miRCURY LNA miRNA Mimic (Qiagen). Cell lysates were prepared 24 hours later in passive lysis buffer following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... we transfected AFSCs at 60% confluence with miR-93-5p inhibitor (25 μM) according to the manufacturer’s instructions (Qiagen Y104101029- DDA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA samples were amplified in technical duplicates in presence of gene specific primers (QuantiTect® Primer Assays, Qiagen) and QuantiTect® SYBR Green Master Mix (Qiagen ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 replication was quantified via RT-qPCR using the QuantiTect Multiplex RT-qPCR Kit (Qiagen) with a Rotor Gene Q cycler (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... using gene specific primers (QuantiTect®, Qiagen) and SyBr Green PCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and commercially available miScript primer assays (Qiagen) targeting miR-39 and the 5 top ranked candidate miRNAs ...
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bioRxiv - Cancer Biology 2020Quote: ... The following RT2 qpcr primer assays (Qiagen) were used ...