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Citations for Qiagen :
1151 - 1200 of 6183 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Differentially expressed gene sets were analyzed using Ingenuity Pathway Analysis Software (Qiagen). Gene sets were uploaded into IPA for Core Expression Analysis of expression data ...
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bioRxiv - Genetics 2023Quote: ... DNA contamination was further removed by the RNase-Free DNase Set (QIAGEN). RNA libraries were prepared with Illumina TruSeq Stranded mRNA (poly-A selection ...
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bioRxiv - Microbiology 2023Quote: ... with on-column DNase digestion using RNase-Free DNase Set (QIAGEN 79254). cDNA was synthesized with random hexamers from the Superscript III First-Strand Synthesis System (Invitrogen 18080051) ...
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bioRxiv - Microbiology 2023Quote: ... The siRNAs were selected from the FlexiTube GeneSolution 4 siRNA sets (Qiagen) and transfected as a mix at 24 nM in Calu-3 and 10 nM in Caco-2 following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... DNase treatment was performed on-column using RNase-Free DNase Set (QIAGEN). cDNA was generated from 200ng-1ug RNA by using SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme Kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was removed from samples using the RNase-free DNase Set (QIAGEN). RNA quantity and quality was assessed using the Agilent 2100 Bioanalyser in conjuction with the RNA 6000 PicoLabChip Kit following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and on-column DNase digestion with the RNAse-Free DNase Set (QIAGEN). Purity and concentration of the extracted RNA were measured using Nano-Drop lite Spectrometer (Thermo-Fisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and on-column DNA digestion with the RNase-free DNase set (Qiagen). In order to remove any remaining DNA contamination ...
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bioRxiv - Plant Biology 2023Quote: ... followed by DNase digestion with RNase free DNase Set (Qiagen, Hilden, Germany). RNA samples were quantified on Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... with on-column DNA digestion using RNase-Free DNase Set (Qiagen, Germany). Purified RNA was quantified using QubitTM RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... including digestion of contaminating DNA using an RNase-free DNase set (QIAGEN). RNA was diluted from the columns in ultrapure distilled water ...
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bioRxiv - Immunology 2024Quote: ... with on-column DNase digestion using RNase-Free DNase Set (QIAGEN 79254). cDNA was synthesized with random hexamers from the Superscript III First-Strand Synthesis System (Invitrogen 18080051) ...
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bioRxiv - Molecular Biology 2021Quote: Cells were seeded in 6-well and 12-well plates at a density of 1.25×105-5×105 cells per well and transfected with miR-320a mimic (Qiagen, 339173 YM00471432) or antagomir (GenePharma ...
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bioRxiv - Neuroscience 2021Quote: ... double-digoxigenin locked nucleic acid probe (RNO-MIR-124-3P: CATTCACCGCGTGCCTTA, Tm: 84°C, 339111 YD00614870-BGC, miRCURY LNA™ miRNA Detection Probe, Qiagen) was used at a final concentration of 30 nM ...
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bioRxiv - Developmental Biology 2019Quote: We used a kit to detect mmu-miR-126-3p by in situ hybridization (catalog number 339111, Qiagen, Germantown, MD, USA). Briefly ...
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bioRxiv - Genomics 2022Quote: ... The sample was incubated at room temperature for 5 min and 3.75μL (25 fmol final concentration) of the synthetic spike-in control Caenorhabditis elegans miRNA cel-miR-39 (Qiagen, Cat. No. 219610) was spiked into samples ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... The human U6 primer used in this experiment was included in the miScript Primer assay kit (Qiagen Inc., Germantown, MD, #218300).
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bioRxiv - Immunology 2021Quote: ... 3.5 μl of cDNA was used as template and amplified in a total volume of 40 μl with a mix of forward L-VH primers (Table S1) and reverse Cγ primer and using the HotStar® Taq DNA polymerase (Qiagen) and 50 cycles of PCR (94°C 30 s ...
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bioRxiv - Immunology 2023Quote: ... 3.5 μl of cDNA was used as template and amplified in a total volume of 40 μl with a mix of forward L-VH primers (Table S3) and reverse Cγ primer and using the HotStar® Taq DNA polymerase (Qiagen) and 50 cycles of PCR (94°C 30 s ...
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bioRxiv - Molecular Biology 2019Quote: Reverse transcription (RT) of total RNA to cDNA was performed in 50 μL reaction volumes using the miRCURY RT kit (Qiagen) and 10 μL total RNA as input ...
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bioRxiv - Cancer Biology 2023Quote: ... was reverse transcribed into cDNA by miScript RT II Kit using miScript HiSpec Buffer or miRCURY LNA RT Kit (Qiagen). Expression levels of miR-142-5p were analyzed using miScript PCR System or miRCURY LNA SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... Two μL of isolated RNA were used for reverse transcription (RT) and cDNA synthesis using the miRCURY LNA RT Kit (Qiagen) according to the manufacturer’s instructions adding the RNA spike-in UniSp6 as quality control for RT ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with Omniscript RT Kit (Qiagen). The relative mRNA expression level of each target gene was quantified by qPCR using KAPA SYBR fast qPCR master mix (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
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bioRxiv - Microbiology 2020Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
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bioRxiv - Microbiology 2021Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
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bioRxiv - Cell Biology 2021Quote: ... or miRscript RT kit II (Qiagen, Ref. 218161), respectively ...
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bioRxiv - Immunology 2021Quote: ... reverse transcribed into cDNA (QuantiTect RT kit, Qiagen) and analyzed by RT-qPCR.
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bioRxiv - Molecular Biology 2024Quote: ... and the miRCURY LNA RT Kit (Qiagen, UK) was used to conduct qPCR in the Roche ® LightCycler® 480 according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.45 μL of OneStep RT Mix (Qiagen, Germany), 8.4 μL of HDPCR Mix ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized using Sensiscript RT Kit (QIAGEN) following the manufacturer’s instruction ...
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bioRxiv - Physiology 2024Quote: ... 0.1 μl of QuantiFast RT Mix (Qiagen, Germany) and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen ...
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bioRxiv - Bioengineering 2022Quote: ... Following seeding MSCs were transfected with 20nM of either pBAE:miR-29b (miR-29b) complexes or pBAE:siRNA (Scramble) complexes (Qiagen, AllStars Negative Control siRNA) or cultured in standard control medium (control ...
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bioRxiv - Cancer Biology 2023Quote: 1×104 SiHa or 1×103 Hela cells/6-well were seeded and transfected with pSG5-miR-142-5p expression vector or pSG5 empty vector as a control using PolyFect Transfection Reagent (Qiagen, Hilden, Germany). After 48 h ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was removed using an RNase-free DNase set (Cat# 79254, Qiagen). The cDNA was synthesized via treatment with reverse transcriptase and oligo(dT ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting gene sets were analysed using Ingenuity Pathway Analysis (IPA) software (Qiagen) (Kramer et al. ...
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bioRxiv - Immunology 2020Quote: ... with an additional DNase digestion step using the RNase-free DNase set (QIAgen). Transcriptional profiling was done using the CEL-seq2 protocol (29 ...
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bioRxiv - Neuroscience 2021Quote: ... using the RNeasy micro kit and RNAase-free DNase set (both from Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... including an on-column DNase treatment with the RNase-Free DNase Set (Qiagen). Isolated RNA was transcribed into cDNA with the High-Capacity RNA-to-cDNA Kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... extracted using the genomic tip-20 and genomic buffer set kits (Qiagen, Germany), to confirm their specificity ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was removed by using the RNase-Free DNase Set (Qiagen, #79254). RNA was reversed transcribed by iScriptTM Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... including on-column DNase digestion to eliminate DNA (Rnase-Free DNase Set, Qiagen). RNA quantification was then performed using the Spectrophotometer ND-1000 (NanoDrop) ...
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bioRxiv - Genomics 2022Quote: ... with on-column DNA digestion using the RNase-free DNase I set (Qiagen). For the tongue ...
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bioRxiv - Genomics 2022Quote: ... including an on-column DNase digest using the RNase free DNase set (Qiagen). The quantity and quality of the extracted RNA were determined by Nanodrop 2000c (Thermo Fisher Scientific) ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: TRIzol extracted lung RNA was treated with DNase (RNase-Free DNAse Set (Qiagen)) followed by purification using RNeasy MinElute Cleanup Kit (QIAGEN ...
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bioRxiv - Molecular Biology 2020Quote: ... Membrane-bound genomic DNA was digested with RNase-Free DNase Set (Qiagen, 79254). First strand cDNA was synthesised using a ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... DNase I treatment was additionally performed using the RNase-free DNase set (Qiagen). RNA quality was checked using an Agilent 2100 Bioanalyzer instrument ...
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bioRxiv - Plant Biology 2021Quote: ... On-column DNase digestion was performed using the RNase Free DNase Set (Qiagen). RNA concentration and purity were determined by using the Spark 10M (Tecan ...