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Citations for Qiagen :
751 - 800 of 6183 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... 5 μl RNA was used in a one-step real-time RT–PCR E assay using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... Five μl RNA was used in a one-step real-time RT-PCR using the Rotor-Gene probe kit (Qiagen) according to instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR was performed as previously described9 with miRCURY LNA RT and SYBR Green PCR miRNA kits and miRCURY LNA probes for miRNAs (Qiagen) and High-capacity cDNA transcription kit and Fast SYBR Green (Qiagen ...
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bioRxiv - Immunology 2021Quote: Cytokines and cellular sensors were measured in neonatal lung homogenates (cDNA prepared as per viral titres) by RT-qPCR using QuantiFast SYBR Green PCR Kit (Qiagen) and QuantiTect Primer Assays (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed with a primer set targeting partial regions of the ORF1b gene in the SARS-CoV-2 virus using the QIAGEN OneStep RT-PCR kit (Qiagen) as previously reported (51) ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
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bioRxiv - Bioengineering 2023Quote: Reverse transcription of isolated miRNAs for quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was performed with a miScript II RT Kit (Qiagen) for selected miRNA targets ...
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bioRxiv - Microbiology 2023Quote: A 388 bp region surrounding NS4A position 19 was amplified from total RNA extracted from a subset of brains using OneStep RT-PCR (Qiagen) using the following primers ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 ng of total RNA was used for the retrotranscription (RT) reaction using the miRCURY LNA miRNA PCR system (Qiagen). After dilution of the resulting cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... DNase (RNase-Free DNase Set, Qiagen, catalog#:79254) was used to degrade DNA during the extraction as outlined in the RNeasy Mini Handbook ...
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bioRxiv - Genetics 2021Quote: ... with RNase-Free DNase Set (Qiagen no. 79254) according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and the RNase-Free DNase Set (#79254; Qiagen). The isolated heart RNA was converted into cDNA by QuantiTect Reverse Transcription Kit (#205311 ...
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bioRxiv - Immunology 2022Quote: ... treated with DNase (RNase-Free DNase Set, Qiagen), and reverse transcribed to generate cDNA with High-Capacity RNA-to-cDNA™ Kit (Themo-Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... RNase-Free DNase Set (Qiagen, cat no. 79254) was used for further DNase digestion ...
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bioRxiv - Plant Biology 2019Quote: ... and treated with RNase-Free DNase Set (QIAGEN). Reverse transcription was carried out using the SuperScript IV Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Neuroscience 2019Quote: ... or with RNase-Free DNase Set (Qiagen, #79254) (larval OSN nuclei samples) ...
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bioRxiv - Microbiology 2020Quote: ... was treated with RNase-Free DNase Set (Qiagen) according to the manufacturer’s protocol to remove possible traces of genomic DNA.
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bioRxiv - Genetics 2021Quote: ... and treated with RNase-free DNase Set (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Following DNase treatment (RNAseq-Free DNAse Set (Qiagen)) and RNA purification (RNeasy MinElute Kit) ...
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bioRxiv - Plant Biology 2021Quote: ... along with the RNase-free DNase Set (Qiagen). We constructed sequencing libraries with the KAPA Stranded mRNA-seq Kit (KAPA Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... and the DNAse RNase-Free Set (Qiagen, Netherlands) according to manufacturer’s instructions and as described previously (12) ...
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bioRxiv - Plant Biology 2021Quote: ... and treated with RNase-Free DNase Set (QIAGEN). Reverse transcription was carried out using the SuperScript IV Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Genetics 2020Quote: ... in conjunction with RNase-Free DNase Set (Qiagen). cDNA was generated using the High-Capacity RNA-to-cDNA™ Kit (Applied Biosystems).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... combined with the RNase-Free DNase Set (Qiagen). We stored RNA at -70°C ...
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bioRxiv - Cell Biology 2022Quote: ... DNAwas removed (RNase-Free DNase Set; Qiagen, Japan) and RNA recovered by column purification (QIAquick PCR Purification Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... QIAshredder Columns and RNase-Free DNase Set (Qiagen). RNA-Seq library construction and sequencing were performed according to protocols used by the integrated genomics operation (IGO ...
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bioRxiv - Cancer Biology 2022Quote: ... QIAshredder Columns and RNase-Free DNase Set (Qiagen). cDNA synthesis was performed using TaqMan® Reverse Transcription Reagents (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... with DNaseI treatment (RNase free DNase Set, Qiagen). RNA quality was confirmed by RNA Tapestration and quantified with Qbit ...
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bioRxiv - Genetics 2022Quote: ... with the RNase-Free DNase Set (Qiagen, 79254). The quality of the RNA was assessed using the Agilent TapeStation system and quantified by Nanodrop ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was isolated using the miRNeasy Micro Kit from Qiagen and the RNase-free DNase set from Qiagen according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2024Quote: ... and purified using RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... following DNA digestion (RNase-free DNase set, Qiagen) and by using a Qiacube instrument ...
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bioRxiv - Immunology 2023Quote: ... with the RNase-Free DNase Set (Qiagen #79254) to remove any residual DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... and the RNase-Free DNase Set (Qiagen, 79254) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... including the DNase (RNase-Free DNase set, Qiagen) treatment to remove residual DNA ...
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bioRxiv - Genetics 2020Quote: ... 1μl of bisulfite converted DNA was used as template to amplify target genomic regions using specific primers (one biotinylated) with the PyroMark PCR kit (Qiagen, #978703), following the recommended conditions (annealing temperature 56°C) ...
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bioRxiv - Microbiology 2019Quote: ... the 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... assay targeting the paramyxovirus L protein gene was adapted using primers developed by Tong et al (36) and the Qiagen AllTaq PCR Core kit (QIAGEN, Australia). Amplicons corresponding to the expected size (584 bp ...
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bioRxiv - Biophysics 2021Quote: ... A small volume of this reaction was then used to seed a PCR reaction using primers prAH869 and prAH870 in Q5 High-Fidelity 2X Master Mix (Qiagen M0492). Products were purified by RNAClean XP magnetic beads (Beckman-Coulter A63987 ...
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bioRxiv - Microbiology 2020Quote: ... PCR replicates were pooled and remaining primers were enzymatically digested as indicated above and purified using QIAquick PCR Purification Kit (Qiagen, Germany). PCR-products were quantified (NanoDrop 2000c ...
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bioRxiv - Cancer Biology 2022Quote: ... Synthesis of cDNA and qRT-PCR were performed as previously described (50) using the following primers (all from Qiagen, Limberg, Netherlands): ATF4 (QT00074466) ...
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bioRxiv - Neuroscience 2022Quote: ... Five μl of reverse transcribed cDNA samples were pre-amplified using RT2 preamp PCR master mix with PBR-152Z RT2 preamp pathway primer mix (Qiagen, Inc.) on a thermal cycler under the following conditions ...
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bioRxiv - Neuroscience 2023Quote: ... A single amplicon of 209 bp (chr6:32063788-32063996) was PCR-amplified using primers designed with the PyroMark Assay Design software 2.0 (Qiagen, Hilden, Germany) (Supplementary Table 10) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ten µl PCRs containing 1 µl of template DNA (10–50 ng of DNA from each fire coral sample) and 0.25 µM of each primer were run in triplicate per sample using the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Thermal cycler conditions for ITS2 PCR amplification were 95°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... commercially obtained hsa-let-7a-5p or hsa-miR-205-5p miRCURY LNA miRNA Inhibitor (Qiagen, cat# YI04101776 and YI04101508 ...
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bioRxiv - Cell Biology 2019Quote: ... Cy3-labeled miR-430 was separated from unconjugated dyes using a QIAquick Nucleotide Removal Kit (QIAGEN).
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bioRxiv - Genomics 2022Quote: ... DE cells were treated with 500 nM LNA miR-182 inhibitor (Qiagen, Hilden, Germany; #4101243-101) or scrambled control (Power Negative Control A ...
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bioRxiv - Physiology 2022Quote: ... we spiked in miR-39 as an exogenous housekeeping miRNA control prior to extraction (Qiagen #219610). TaqMan primers were obtained from Life Technologies for miRNAs 106b 5p (#000442) ...
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bioRxiv - Neuroscience 2024Quote: ... mHypoE-N46 cells were transfected with a miR-505-5p mimic (miRCURY LNA miRNA mimic, Qiagen) or negative control ...