Labshake search
Citations for Qiagen :
751 - 800 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from an overnight culture using the QIAGEN genomic tip 100/G kit (Qiagen®, #10243) according to manufacturing protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Viral RNA from supernatants were isolated from 100 μL supernatant using the QIAamp Viral RNA Mini Kit (Qiagen) and viral RNA from cell lysate was extracted using the Nucleospin RNA plus mini kit (Macherey-Nagel ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA from the pooled library samples was extracted using a Qiagen Genomic-tip 100/G kit (Qiagen) and used to prepare TraDIS sequencing libraries as previously described (25) ...
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bioRxiv - Microbiology 2023Quote: ... 100 mg of each scat sample was also ground into a fine powder using the TissueLyser II (Qiagen) except that each sample was bead beaten three times after snap freezing in liquid nitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 100 mg of fresh tumor tissue was collected and processed using the RNeasy Mini Kit (#74104 Qiagen) for RNA extraction following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from 100 mg of roots or shoots using the RNeasy Plant Mini Kit (Qiagen) following the supplier’s instructions ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted from the stored 100 μl leukocyte-depleted RBCs using the QIAamp DNA mini kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Approximately 100 mg of starting material was used for RNA isolation using the RNeasy Plant Mini Kit (Qiagen) for N ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 100 mg of 0.1 mm zirconia/silica beads (Stratech) were added before homogenization using a TissueLyser II (Qiagen) for 120 seconds at 30 Hz ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed using 100/G Genomic-tips together with a Genomic DNA buffer set as described in the manufacturer’s instructions (Qiagen). After precipitation ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS and incubated at 37°C with a 1:100 dilution of RNase A (QIAGEN) in PBS for 3 hours ...
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bioRxiv - Genomics 2020Quote: ... The worm pellet was flash frozen and genomic DNA was isolated using the Genomic-tip 100/G column (Qiagen). Four micro-grams (average size 23Kb ...
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bioRxiv - Microbiology 2019Quote: Total RNA was extracted from each sample according to the manufacturer’s recommendations using 100 µL of Viral Transport Media as input for the RNeasy Micro Kit (Qiagen). Influenza genomic RNA was subsequently converted into cDNA and amplified (40 cycles of PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... About 100 females were collected and RNA was extracted using the Qiagen RNeasy PLUS Kit (Qiagen Benelux, Venlo, Nederland). One μg of total RNA was used as template for synthesizing cDNA with the Maxima First Strand cDNA synthesis Kit for RT-qPCR (Fermentas Life Sciences ...
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bioRxiv - Genomics 2019Quote: ... Each dataset was mapped to the to the predicted gene sets (NCBI Annotation Release 100) using CLC Genomics (Qiagen). Reads were mapped with the following parameters ...
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bioRxiv - Neuroscience 2019Quote: ... 6 replicates with 4 pupae in each were collected and then homogenized in PBS + 0.05% Triton-X 100 with a tissuelyser II from Qiagen. The TAG levels was determined with a Liquick Cor-TG diagnostic kit (Cormay ...
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bioRxiv - Neuroscience 2020Quote: Each DNA sample (~100 μl of fecal DNA) was extracted using the DNeasy Blood & Tissue kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was isolated from 100 μl of HSS extracts according to the RNeasy kit “RNA clean up” protocol (Qiagen), precipitated in the presence of glycogen (MilliporeSigma) ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA for genotyping was extracted by grinding ∼100 mg of leaf tissue in a homogenizer (Retsch mill, QIAGEN) for 30 s and 30 rpm ...
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bioRxiv - Microbiology 2021Quote: ... 100 μl of total RNAs were extracted from 5 × 105 replicon cells using RNeasy mini kit (Qiagen, Gaithersburg, MD). 2□μl of each sample was used to assess RNA quality using Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The resultant washed DNA (100 μl) was then cleaned with a DNeasy PowerClean Pro Cleanup Kit (Qiagen 12997-50).
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bioRxiv - Cancer Biology 2022Quote: ... each well was incubated in 75 μL 0.08 M KOH for 5 min and washed with 100 μL EB buffer (QIAGEN) at room temperature (RT) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1×104 cells of each sample were lysed in 100 µl of RLT Plus Lysis Buffer (Qiagen, Hilden, Germany) supplemented with 1% 2-mercaptoethanol ...
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bioRxiv - Pathology 2020Quote: ... microRNAs were determined as previously published from 100 pg of cDNA synthesized with the miScript RT kit (Qiagen, Milan). Real time PCR were analyzed using the relative quantification method previously described (23).
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bioRxiv - Microbiology 2020Quote: ... and IMX2137 was isolated with a Blood & Cell Culture DNA Kit with 100/G Genomics-tips (QIAGEN, Hilden, Germany) according to the manufacturers’ protocol ...
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bioRxiv - Genomics 2019Quote: ... the fragmented DNA was concentrated to 20 µl in 100 mM NaPi buffer (pH 8.0) using QIAquick PCR purification kit (Qiagen). A 100-µl volume of 100 mM NaPi buffer containing 20 mM amine-PEG2-biotin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was performed using 100/G Genomic-tips together with a Genomic DNA buffer set as described in the manufacturer’s instructions (Qiagen). After precipitation ...
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bioRxiv - Genomics 2021Quote: High molecular weight DNA was extracted from 100 µl of blood using the DNeasy Blood and Tissue kit (Qiagen). For long read (Oxford Nanopore ...
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bioRxiv - Zoology 2021Quote: ... The DNA elution was done using 100 µl Elution buffer from DNeasy blood and tissue kit (Qiagen, Hilden, Germany). All steps of DNA extraction were carried out in a sterile and dedicated laboratory to avoid any contamination ...
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bioRxiv - Cancer Biology 2020Quote: ... was co-transfected with 100 ng of pmax-mScarlet-H2A (nuclear marker) using 1.5 µL Attractene transfection reagent (Qiagen). Microscopy images were taken with the iRiS Digital Cell Imaging System (Logos Biosystems).
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted and eluted in 100 uL RNase-free water using an RNeasy Mini Kit (Qiagen, 74104) with on-column DNase I digestion (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Second-strand synthesis reaction was carried out in 100 μl using 10 pmol of each primer (HotStarTaq Plus, Qiagen). Residual primers and dNTPs were again degraded enzymatically (ExoSAP-IT ...
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bioRxiv - Genomics 2022Quote: Long fragment DNA extraction from sub-cultured strains was performed using the Qiagen Genomic tip 100/G kit (Qiagen). Quality and fragment length assessments were measured with the Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... RNA was extracted from 50-80 mg of surface-sterilised (70% EtOH, 0.1% Triton X-100) and 3-days germinated seeds with RNeasy Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from 100 mg of seedlings at 3 dpg by means of the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... 100 mg of cryo-ground leaf powder was used to isolate total RNA using the RNAeasy kit from QIAGEN. The quality of total RNA was measured by evaluating of RNA integrity number (RIN ...
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bioRxiv - Genetics 2023Quote: ... leaf tissues were collected and homogenized in 400 μL of TPS buffer (100 mM Tris-HCl, 10 mM EDTA, 1 M KCl, pH8.0) using TissueLyser II (Qiagen), followed by incubation for 20 min at 75°C ...
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bioRxiv - Cancer Biology 2023Quote: ... mixed with 100 μl of serum-free medium and 12 μl of HiPerfect Transfection Reagent (Qiagen, Germantown, MD, USA). Effectiveness of ARSB silencing was confirmed by activity assay ...
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bioRxiv - Genomics 2022Quote: ... Approximately 100 mg of plant material was used for DNA extraction using a DNeasy Plant Mini Kit (QIAGEN, Australia) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: RNA was extracted from frozen adipose tissue pieces (50-100 mg) with the RNeasy Lipid tissue Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: The total RNA was isolated from 50-100 mg of cells using RNeasy Plant Mini kit (Qiagen, Düsseldorf, Germany) and treated with DNA-Free kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA from dissected third instar larval brains (around 100 per sample) was extracted using DNeasy Blood & Tissue Kit (Qiagen), digested with RNAse A and RNAse H for 3 h ...
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bioRxiv - Genomics 2023Quote: ... A total of 350Lμl of 100% ethanol was added to the sample and loaded onto an RNeasy MinElute column (QIAGEN). RNA was isolated using RNeasy Mini kit (#74104 ...
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bioRxiv - Cancer Biology 2023Quote: ... Crude eccDNA was then eluted by using 100 µL of 0.1x EB buffer (From QIAGEN Plasmid Plus Midi Kit). For each microgram crude eccDNA we spiked in 1 ng pUC1932 (was a gift from Joachim Messing ...
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bioRxiv - Biochemistry 2023Quote: ... the bead slurry was spun down and resuspended in 100 µl wash buffer and digested with proteinase K (Qiagen) at 50 °C for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 200 μl 100% ethanol was added dropwise while being gently vortexed and placed into a RNeasy Mini column (Qiagen), The remainder of the purification followed the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: From pellets of 100 million cells genomic (g)DNA was extracted using the QIAamp DNA Blood Maxiprep Kit (Qiagen) according to manufacturer’s instructions with the following modifications ...
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bioRxiv - Genomics 2024Quote: ... Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen, Valencia, CA) and kept on ice until use ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was loaded over 3 ml of Ni-NTA beads (Qiagen) equilibrated in lysis buffer ...