Labshake search
Citations for Qiagen :
1001 - 1050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested and total RNA isolated using an RNA isolation kit (RNeasy Mini kit, Qiagen, 74106). 8 µg of total RNA were treated with DNaseI and purified according to the manufacturer’s protocol (TURBO DNA-free kit ...
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bioRxiv - Cell Biology 2024Quote: RNeasy Micro Kit (Qiagen; cat. #74004) was used to extract whole cell RNA ...
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bioRxiv - Neuroscience 2024Quote: ... and purified with RNeasy Micro Kits (Qiagen). Separate cohorts of mice were used for qPCR and sequencing experiments ...
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bioRxiv - Neuroscience 2024Quote: ... RNA from samples was isolated using the RNeasy Plus Mini kit (Qiagen, 74136). Template cDNA was prepared by reverse transcription using the SuperScriptTM III First-Strand Synthesis SuperMix for qRT-PCR (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The total RNA was isolated from 30 mg cortical gray matter using the RNeasy Mini kit (Qiagen) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... frozen specimens were thawed on ice and the white matter was carefully removed with a scalpel in RNAlater (Qiagen). The total RNA was isolated from 30 mg cortical gray matter using the RNeasy Mini kit (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... using 5-mm stainless steel beads (Qiagen) at 25 Hz for 1.5-3min (Qiagen/Retsch Bead Beater) ...
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bioRxiv - Neuroscience 2024Quote: ... at 25 Hz for 1.5-3min (Qiagen/Retsch Bead Beater). Following homogenization samples were centrifuged at 16,000 x g for 10 mins at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Hybridization was performed at 66 °C overnight with 40 nM 5′ TYE-563-labelled locked nucleic acid (LNA)-(C4G2)2.5 probe (Exiqon Qiagen). Cells were then washed once in 2X SSC/0.1% Tween-20 for 5 minutes and three times in 0.1X SSC for 10 minutes at RT before being dehydrated as above and nuclei stained with DAPI.
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bioRxiv - Neuroscience 2024Quote: ... RNA was collected by using the RNeasy Minit kit (Qiagen, Venlo, Netherlands) and the RNA concentration was determined with a NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Ingenuity Pathway Analysis (IPA; Qiagen) was used to predict upstream regulators associated with DEGs (25% fold change (logFC ...
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bioRxiv - Microbiology 2024Quote: ... before proceeding according to the protocol and eluting in 100 µL of elution buffer (Qiagen). DNA yields were quantified via fluorometry (Qubit 2.0 ...
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bioRxiv - Microbiology 2024Quote: ... Products were then washed multiple times with 80% ethanol and the dried pellet was resuspended in 32.5 µL EB buffer (Qiagen), incubated for two minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... except that samples were homogenized in bead tubes using a TissueLyser II (Qiagen) for ten minutes at 30/sec ...
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bioRxiv - Microbiology 2024Quote: ... one fecal pellet per procedure was placed in a 2 mL round-bottom tube containing a 0.5 cm diameter stainless steel bead and 500 µL of sterile phosphate-buffered saline (PBS) and agitated at 1/30s using a TissueLyser II (Qiagen) for 60 seconds ...
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bioRxiv - Microbiology 2024Quote: Fecal DNA was extracted using PowerFecal kits (Qiagen) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: Total DNA was extracted from gut feces samples collected using a QIAamp Fast DNA Stool Mini Kit following the manufacturer’s recommendations (QIAGEN GmbH, Germany). Then ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted with the RNeasy kit (Qiagen, Hilden, Germany). Additionally ...
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bioRxiv - Molecular Biology 2024Quote: ... RNAs were extracted using RNeasy Mini Kit (Qiagen) and infection confirmed using RT-qPCR quantification of viral RNAs using specific primers pairs ...
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bioRxiv - Molecular Biology 2024Quote: ... and total cellular RNA was purified using RNeasy Mini Kit (Qiagen) and RNase-free DNase Set (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA pellet was resuspended with 50 ul EB buffer (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted by first lysing cells in Qiazol (Qiagen) and subsequently using RNAeasy mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted with RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and checked for quality with Agilent RNA 6000 Nano Kit (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Adapter ligated DNA was purified by AMPure XP and eluted with 100 ul EB buffer (Qiagen, 19086). 10 μl Dynabeads™ M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... and subsequently using RNAeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Forward and reverse sequences for each sample was aligned using the CLC Main Workbench V 21.0.5 (QIAGEN). The consensus sequences (contigs ...
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bioRxiv - Genetics 2024Quote: ... the cells were lysed in 1 mL of RLT buffer obtained from Qiagen, and for Mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: ... the remaining wash buffer was removed and 1 ml QIAzol (Qiagen) was added to the beads and incubated at RT for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Viral RNA was amplified on a RotorGene (Qiagen) thermalcycler following the next program ...
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bioRxiv - Genetics 2024Quote: ... and genomic DNA was extracted using the QIAamp DNA Blood mini kit (Qiagen, Inc., Valencia, CA) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... with ceramic beads using the TissueLyser (Qiagen Inc.). Genomic DNA was extracted using the Gentra Puregene Tissue Kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were transferred to QIAquick tubes for purification according to QIAquick PCR purification kit protocol (Qiagen). Purification was performed according to the manufacturer’s instructions [34] ...
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bioRxiv - Molecular Biology 2024Quote: ... plasmid purification was done using the QIAprep Spin Miniprep Kit (QIAGEN GmbH).
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bioRxiv - Molecular Biology 2024Quote: ... ING2 LNA Gapmer (TACGTTGGCTTGTTCA) was designed and obtained from Qiagen and reconstituted in nuclease-free water (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... and the miRCURY LNA RT Kit (Qiagen, UK) was used to conduct qPCR in the Roche ® LightCycler® 480 according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA fragments were purified using MinElute columns (Qiagen). Libraries were prepared using NEBNext Ultra II DNA Library prep kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 nM of a miR-96-5p inhibitor or 100nM of a scrambled inhibitor (control) (Qiagen, UK).
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bioRxiv - Neuroscience 2024Quote: ... Genomic bacterial DNA was extracted from these frozen stool samples utilizing the QIAamp PowerFecal Pro DNA Kit (Qiagen, Germantown, MD). To facilitate DNA extraction ...
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bioRxiv - Developmental Biology 2024Quote: ... The transcribed mRNA was purified using RNeasy mini kit (QIAGEN).
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was simultaneously isolated from all samples using the RNeasy Mini Kit (Qiagen, Inc., Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA isolation was performed using QIAzol Lysis Reagent and the miRNEasy Mini Kit including the RNAse-free DNase Set (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA containing RNA–DNA hybrids was isolated from HeLa cells transfected with a pFlag-IN codon-optimized plasmid using a QIAmp DNA Mini Kit (Qiagen, 51304). gDNA was sonicated for 10 min (Diagenode Bioruptor ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: Total gDNA was extracted using the QIAmp DNA Mini Kit (Qiagen, 51304) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from primary solution with the removal of genomic DNA by the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). The purity and concentration of extrasted totel RNA were confirmed using a NanoDrop ONE spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Histones were digested by Protease (Qiagen 19155) for 30 min at 50 °C and DNA fragments were purified using Sera Mag magnetic beads in 30% PEG ...
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bioRxiv - Genomics 2024Quote: ... and purified using a QIAquick gel extraction kit (Qiagen). The linearized vector was assembled with the amplified oligo pool using the NEBuilder HiFi DNA assembly kit (NEB ...
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bioRxiv - Genomics 2024Quote: ... The products of four independent PCRs were combined and purified using MinElute columns (Qiagen, Germantown, MD).
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bioRxiv - Genomics 2024Quote: ... mRNA purification was performed with RNeasy Pure mRNA Bead Kit (Qiagen Cat. No. 180244). 28 ng mRNA was used to synthesize cDNA using 50 μM Oligo(dT)20 primers ...
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bioRxiv - Genomics 2024Quote: ... DNA was isolated using the gravity flow Genomic Tip kit (Qiagen, cat. no. 10223) and by the phenol-chloroform method 17 ...
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bioRxiv - Genomics 2024Quote: RNA extraction was carried out using the BioRobot EZ1 and EZ1 RNA Universal Tissue kit (Qiagen), followed by DNase treatment using the Ambion DNA-free DNA removal kit (Invitrogen ...