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Citations for Qiagen :
651 - 700 of 1895 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cells or HeLa FlpIn cells were transfected with siRNAs at 100 nM using the HiPerFect (Qiagen), according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... 100 nanograms of cDNA was used for qPCR analysis of the identified genes (Quantitect Primer Assay, Qiagen) using the iQ SYBR green supermix (BioRad Cat ...
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bioRxiv - Microbiology 2023Quote: ... 4sU-RNA was eluted with 100 mM DTT and isolated using the RNeasy MinElute Cleanup Kit (Qiagen). RNA was sent to the NCI CCR-Illumina Sequencing facility for library preparation and sequencing ...
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bioRxiv - Physiology 2024Quote: Total genomic DNA was extracted from each swab using the QIAGEN Power Soil Kit (12888-100, QIAGEN), following the manufacturer’s instructions (20) ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA extraction suitable for PacBio SMRT sequencing was performed using the Genomic-tip 100/G (Qiagen Inc) as described previously (Ngabonziza et al. ...
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bioRxiv - Genomics 2023Quote: ... RNA was eluted with 100 mM DTT and purified using an RNeasy MinElute cleanup kit (Qiagen 74204) as per the the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... About 100 mg of homogenized powder was used for total RNA extraction with the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 nM of a miR-96-5p inhibitor or 100nM of a scrambled inhibitor (control) (Qiagen, UK).
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bioRxiv - Molecular Biology 2024Quote: ... Adapter ligated DNA was purified by AMPure XP and eluted with 100 ul EB buffer (Qiagen, 19086). 10 μl Dynabeads™ M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 µg/mL anti-Penta-His-Alexa Fluor 488 (Qiagen), which recognized the His-tag on PXDN-con4 ...
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bioRxiv - Genomics 2020Quote: ... 5 mL nuclease-free water) instead of customary Elution Buffer (Qiagen) (see Supplementary Material Methods) ...
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bioRxiv - Molecular Biology 2019Quote: ... tumefaciens culture was added to 2 ml RNAprotect Bacteria Reagent (Qiagen) and RNA was isolated using RNeasy columns (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was incubated with 5 mL Ni-NTA resin (Qiagen) for 2 h ...
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bioRxiv - Genomics 2019Quote: ... and 1 μL of 1 AU/mL protease (Qiagen cat # 19157) to each well followed by a 60 min incubation at 55 °C ...
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bioRxiv - Genomics 2021Quote: ... 1% SDS) supplemented with 2µl of 0.5mg/ml RNase A (QIAGEN) at 55°C for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... Supernatant was mixed with 2 mL of Ni-NTA agarose (Qiagen) on a rotary shaker for 1 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied to 1 ml Ni-NTA Agarose (Qiagen) equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was decanted and 1 mL QIAzol Lysis Reagent (QIAGEN) was added to resuspend the cell pellet and protect the RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteinase digestion was performed with 1 mg/mL proteinase K (Qiagen) in the presence or absence of 1% Triton X-100 for 30 min at 37 °C as reported previously64 ...
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bioRxiv - Cell Biology 2022Quote: ... added to 1 ml of washed Ni2+-NTA agarose (Qiagen, #1018244) and rotated on a low speed wheel for at least 1h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Homogenization was performed after adding 1 ml of inhibitex buffer (Qiagen) using the Bead Ruptor Elite bead mill homogenizer (Omni ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml binding buffer (provided in Qiagen PCR purification kit, #28106) and 20 µl of 3 M sodium acetate pH 5.5 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was added to 1 mL Ni2+-NTA resin (Qiagen) and incubated for 1 hr at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... Lysates were incubated with 1 ml of Ni-NTA resin (QIAGEN) in 4°C for 1 hr ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was resuspended in 0.5 ml RNA Later (Qiagen, Germany), stored at 4°C overnight and then transferred to −80°C until time for RNA extraction ...
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bioRxiv - Synthetic Biology 2019Quote: ... was added to a 15 mL polypropylene gravity flow column (Qiagen), storage buffer was allowed to flow through by gravity ...
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bioRxiv - Biochemistry 2019Quote: ... Supernatant was applied to 0.5-1 mL Ni-NTA resin (Qiagen) in gravity columns pre-equilibrated in lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... 8 ml of bed volume of Ni-NTA resin (Qiagen, Germany) was added and incubated for one hour with gentle agitation ...
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bioRxiv - Microbiology 2019Quote: ... The inorganic phase was resuspended in 0.7 ml RLT buffer (Qiagen) supplemented with 1 % β-mercaptoethanol ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was incubated with 3 ml Ni-NTA resins (QIAGEN) with gentle agitation at 4 °C for an hour ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 mL of cells were harvested with RNAprotect Bacteria Reagent (Qiagen) 3-4 h after induction ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was loaded on two 1-ml Ni-NTA (Qiagen 30210) columns ...
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bioRxiv - Biochemistry 2021Quote: ... and then stirred with 2 ml Ni-NTA Superflow resin (Qiagen) that had been pre-equilibrated with Buffer A for 10 min on ice ...
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bioRxiv - Microbiology 2019Quote: ... Proteinase K solution (50 μL at 20 mg/mL, QIAGEN/5PRIME) and SDS solution (100 μL at 20% ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were collected into 2 ml round bottom microcentrifuge tubes (Qiagen) and weighed prior to freezing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... frozen fecal pellets were resuspended in 1.4 mL ASL buffer (Qiagen) and homogenized with 2.8 mm ceramic beads followed by 0.5 mm glass beads using an OMNI Bead Ruptor (OMNI International) ...
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bioRxiv - Biochemistry 2021Quote: ... then supplemented with 15 mL/L settled Ni-NTA resin (Qiagen) at a scale of 12 L ...
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bioRxiv - Developmental Biology 2021Quote: ... Rehydrated embryos were treated with 10 μg/ml proteinase K (Qiagen) in PBS ...
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bioRxiv - Immunology 2021Quote: ... and 50 mg/mL DNase I mix (Qiagen, Germantown, MD, USA) in complete RPMI1640 medium] at 37 °C for 4 hrs ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed using 5-10 ml RBC lysis buffer (Qiagen) and stained for flow cytometry analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen) for 1 hr and washed with 50 mL wash buffer (1X PNI ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded on 5 mL Ni-NTA column (Qiagen) equilibrated with buffer C ...
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bioRxiv - Immunology 2022Quote: ... 2 μg/ml TPCK-trypsin) using a Tissue Lyser II (Qiagen) with the following program ...
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bioRxiv - Evolutionary Biology 2022Quote: ... an aliquot was treated with 0.1 mg/mL RNase A (QIAGEN) at 37 °C for 15 min to digest fluorescently labeled lysine tRNA ...
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bioRxiv - Microbiology 2022Quote: ... and lysate was passed over 8 mL Ni-NTA resin (Qiagen) using gravity chromatography ...
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bioRxiv - Neuroscience 2022Quote: ... and 6μL/sample of 20mg/mL proteinase K (Qiagen, mat #1114886). Lysis completed with a 30-minute incubation at 55°C ...
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bioRxiv - Microbiology 2023Quote: ... DNA was eluted in 25 mL of nuclease-free water (Qiagen). DNA concentration was measured using Qubit dsDNA HS Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was eluted in 25 mL of nuclease-free water (Qiagen). DNA concentration was measured using Qubit dsDNA HS Assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was digested with 0.05 mg/ml RNAse A (Qiagen, 19101) added to the buffer and incubated at 37°C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... approximately 10 mLs were drawn into PAXgene Blood DNA tubes (Qiagen, 761115 ...