Labshake search
Citations for PacBio :
1251 - 1293 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Bacterial DNA methylation analysis was performed by SMRT (Single molecule, real-time, PacBio) and high throughput DNA de novo sequencing ...
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bioRxiv - Microbiology 2020Quote: ... (v0.4.7) and used the most contiguous assemblies (Oxford Nanopore – Illumina for D1,D2,D4,D6,D7,D8 and Pacbio Sequel – Illumina for D3,D5 ...
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bioRxiv - Microbiology 2020Quote: ... we identified 139 (1566 vs. 1705) predicted coding sequences as determined by RAST annotation absent in the assembly received by PacBio sequencing ...
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bioRxiv - Genomics 2020Quote: A newly generated draft WTD genome constructed from long (PacBio) and short-read (Illumina ...
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bioRxiv - Genetics 2020Quote: ... demultiplexed circular consensus (CCS) reads were generated with the RS_ReadsOfInsert.1 protocol from PacBio’s SMRT portal (v2.3.0 ...
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bioRxiv - Genetics 2020Quote: ... 32 μL of this pool (1280 ng) was then used as the input for SMRTBell (Pacbio) library preparation according to the instruction manual and sequenced using a Pacbio Sequel II sequencer.
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bioRxiv - Genetics 2020Quote: ... and long reads (PacBio) from both DNA and RNA ...
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bioRxiv - Genetics 2020Quote: ... Genes found to be L5-specific (present in completed PacBio and Illumina genomes) were also checked for SNPs in these genes using each of the L5 completed PacBio genomes as a reference ...
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bioRxiv - Microbiology 2020Quote: ... generated by PacBio long-read sequencing ...
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bioRxiv - Microbiology 2020Quote: ... samples that were sequenced by PacBio were processed in accordance with the Guidelines for Using a Salt:Chloroform Wash to Clean Up gDNA (Pacific Biosciences).
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bioRxiv - Genomics 2020Quote: Based on the genome assemblies (including the PacBio reference sequences), bacterial characterization was conducted with our in-house developed Bakcharak pipeline (https://gitlab.com/bfr_bioinformatics/bakcharak ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we generated Pacbio corrected reads from the published PacBio RSII reads of argyrosperma (NCBI SRA accession SRR7685401) and the PacBio Sequel reads of sororia (sequenced for this study at the University of Washington PacBio Sequencing Services) using CANU (Koren et al. ...
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bioRxiv - Genomics 2020Quote: ... The sequencing library was prepared using the 20-kb BluePippin kit from PacBio and sequenced on a PacBio RSII instrument at the University of California ...
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bioRxiv - Genomics 2020Quote: ... together with additional full-length cDNA (PacBio Iso-Seq) sequencing performed with the specific aim of generating evidence for the annotation ...
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bioRxiv - Biophysics 2020Quote: ... To allow comprehensive long-read sequencing of the library (PacBio sequel II ...
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bioRxiv - Genetics 2020Quote: ... Program files and a detailed protocol for the in-house pipeline used to analyze CCS reads from PacBio sequencing can be found on https://sites.duke.edu/jinksrobertsonlab/hetdna-mapping/ ...
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bioRxiv - Genomics 2020Quote: ... The three cDNAs were pooled in equimolar ratios and used to prepare a SMRTbell™ library using the PacBio Template Prep Kit (PacBio, Menlo Park, CA). The SMRTbell™ library was then sequenced on a Sequel v2 SMRT cell with polymerase 2.1 and chemistry 2.1 (P2.1C2.1 ...
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bioRxiv - Microbiology 2020Quote: ... 10 and 15 (99, 99.9 and 99.95 base call accuracy, respectively. Nanopore and PacBio (raw and CCS reads) were assembled using the following assemblers ...
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bioRxiv - Genomics 2020Quote: A long-read sequence library was constructed using an SMRTbell Express Template Prep Kit 2.0 and sequenced on SMRT cells (1M v3 LR) in a PacBio Sequel system (PacBio, Menlo Park, CA, USA). The obtained reads (≥15 kb ...
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bioRxiv - Genomics 2020Quote: ... The two fractions were recombined at equimolar quantities and library preparation performed using SMRTbell Template Prep Kit v1.0 (PacBio, USA). Sequencing was performed on the PacBio Sequel 1M SMRT cell ...
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bioRxiv - Genomics 2020Quote: ... Large-scale PCR was subsequently performed using the optimal number of cycles and the resulting amplicons divided into two fractions and purified with 0.4X and 1X Ampure PB beads (PacBio, USA). Quantification and size distribution of each fraction was then determined using the Qubit DNA High sensitivity assay (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... were compared with publicly-available data from PacBio’s Alzheimer’s disease brain Iso-Seq dataset30 ...
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bioRxiv - Genomics 2020Quote: ... A single SMRT cell was used for each library and sequenced on the Sequel system (PacBio) with diffusion loading and a 20 hour movie.
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bioRxiv - Genomics 2020Quote: ... High quality sequence data was proofread and generated by PacBio’s circular consensus sequencing (CCS) ...
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bioRxiv - Microbiology 2020Quote: The genome sequences of four Streptomyces strains were generated using the Pacific Biosciences RS II (PacBio RS II) single molecule real-time (SMRT ...
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bioRxiv - Genomics 2020Quote: We generated circular consensus reads by ccs tool with “--noPolish --minPasses 1” options from PacBio SMRT link tools v.5.1.0 (https://www.pacb.com/support/software-downloads/ ...
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bioRxiv - Microbiology 2020Quote: ... and PacBio RSII (PacBio, Menlo Park, CA) platforms by a commercial service provider (Novogene ...
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bioRxiv - Microbiology 2020Quote: ... and sequenced at Washington State University core facility using one cell of the single molecular real time (SMRT) Pacific Biosciences (PacBio) RSII system.
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bioRxiv - Molecular Biology 2020Quote: ... Equimolar amounts of the products were combined together and purified by AMPure PB beads (PacBio, part no. 100-265-900) following the manufacturer’s recommendation ...
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bioRxiv - Immunology 2020Quote: ... DNA sequencing was completed using SMRT provided by PacBio Sequel ...
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bioRxiv - Evolutionary Biology 2020Quote: ... to the sequences obtained from PacBio. No SNP could be found in any of the replicons ...
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bioRxiv - Genomics 2020Quote: ... according to the protocol for >30 kb SMRTbell Libraries (PacBio, Part # PN 101-024-600 Version 05). Genomic DNA (15 μg ...
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bioRxiv - Genomics 2020Quote: DNA sequencing libraries were prepared using the Template Prep Kit 1.0-SPv3 (PacBio, 100-991-900) according to the protocol for >30 kb SMRTbell Libraries (PacBio ...
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bioRxiv - Genomics 2020Quote: ... and a Sequel SMRT Cell 1M v3 (PacBio, 101-531-000). Library preparation and sequencing was performed at the Institute for Molecular Bioscience Sequencing Facility (University of Queensland).
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bioRxiv - Genomics 2020Quote: ... Long reads from PacBio and ONT were prepared using two or three alternative strategies respectively:
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bioRxiv - Genomics 2020Quote: ... The polymerase-bound library was sequenced on 8 SMRT Cells with a 10 h movie time using the Sequel Sequencing Kit 3.0 (PacBio, 101-597-900) and a Sequel SMRT Cell 1M v3 (PacBio ...
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bioRxiv - Genomics 2020Quote: ... Canu v1.8 (ONT) or v1.9 (PacBio) (Canu ...
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bioRxiv - Genomics 2020Quote: ... it had been assembled by (Schartl et al. 2013) from PacBio Sequel data using HGAP4 (Chin et al ...
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bioRxiv - Genomics 2020Quote: The black rockcod (Notothenia coriiceps) assembly (NCBI accession GCA_000735185.1, BioProject PRJNA66471) was created by (Shin et al. 2014) from PacBio RSII ...
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bioRxiv - Genetics 2020Quote: ... a command-line tool provided by PacBio. All three data sets were run with minimum subread length at 13 kbp ...
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bioRxiv - Microbiology 2020Quote: ... using pacbio-utils version 0.272 (https://github.com/douglasgscofield/PacBio-utilities) and snippy version 4.473 (https://github.com/tseemann/snippy) ...
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bioRxiv - Microbiology 2020Quote: ... eluted in 37 μl EB (10 mM TrisHCl pH 8.0) and processed using SMRTBellTM Template Prep kit 1.0 (PacBio Part# 100-259-100) to SMRTbell libraries ...
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bioRxiv - Microbiology 2020Quote: ... The Illumina reads were mapped to PHM037 genome sequenced by PacBio using the Geneious v.10.0.2 software to manually correct PacBio sequencing errors ...