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Citations for Promega :
4901 - 4950 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: The quantification of dead cells in lung tissue was performed using an in-situ apoptosis detection DeadEnd Colorimetric TUNEL System (Promega) as previously described (88) ...
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bioRxiv - Cancer Biology 2019Quote: Response to a single agent-or combination-treatment was assessed by either the CellTiter 96 cell proliferation assay from Promega. Cells were seeded in 96-well plates (2,000 cells per well) ...
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bioRxiv - Microbiology 2021Quote: ... the MTT assay was conducted using the CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) (Promega; Madison, WI, USA). Cells were plated in a 96-well plate at a concentration of 3 × 106 cells/mL in 100 μl of medium ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μl of cell lysate or medium were subjected to NanoLuc luciferase assay using Nano-Glo Live Cell Reagent containing 1/50 diluted furimazine substrate (Promega). Relative Luminescence Unit (RLU ...
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bioRxiv - Biochemistry 2020Quote: ... cells were lysed in 60 µl of 1x passive lysis buffer of the Dual-Luciferase Reporter Assay System (Promega, E1980) and directly assayed or frozen at −20°C ...
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 5 times with wash buffer and the remaining bound cells were detected with the CellTiterGlo kit (Promega) as per the manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was dispensed in a 96-well white bottom plate at a volume of 80 μL per well and loaded with 20 μL of 25 μM Nano-Glo Vivazine Live Cell Substrates (Promega) diluted in the assay buffer ...
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bioRxiv - Immunology 2022Quote: ... Cells were transferred to a white-walled 96-well assay plate and mixed with an equal volume of Cell Titer Glo reagent (Promega). Measurements for fluorescence at 544-15 nm (excitation) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell lysis and luciferase assays were performed using the Dual-Luciferase® Reporter Assay System kit (Promega Corporation, Madison, WI). Luciferase units were measured with a Spark microplate reader (Tekan Group Ltd. ...
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bioRxiv - Cell Biology 2022Quote: Halo-MDC1 deletion mutants were expressed transiently by transfecting ∼ 5 × 105 cells with 1 µg of plasmid DNA using FuGene 6 (Promega). For genome editing ...
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bioRxiv - Microbiology 2022Quote: ... Infected cells were incubated for 48 hours (hrs) prior to measurement of cell viability protected from VEEV-induced CPE was performed using CellTiter-Glo (Promega).
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bioRxiv - Microbiology 2022Quote: ... CrPV infection was performed at MOI 20 for 6 hours and then cells were lysed in passive lysis buffer (Promega) and luciferase activities measured by a dual-luciferase assay (Promega).
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bioRxiv - Microbiology 2022Quote: ... The resultant plasmid and Bsu36I-digested BmNPV-abb genome DNA [47] were cotransfected into BmN-4 cells using FuGeneHD (Promega). The recombinant virus was purified twice using the plaque assay by identifying OB-producing plaques ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium with 1:500 Enduren luciferase substrate (Promega) added ...
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bioRxiv - Microbiology 2022Quote: In vitro translation assays of the wild type or mutant CrPV-1A RNAs were performed in Spodoptera frugiperda 21 (sf-21) insect cell extract (Promega). Briefly ...
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bioRxiv - Immunology 2022Quote: ... pHR-based lentiviral plasmids containing these transgenes were transfected into HEK293T cells together with pMD2G and psPAX2 helper plasmids using Fugene (Promega). After 2 days ...
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bioRxiv - Cancer Biology 2022Quote: HEK293 cells stably expressing pFN31K-Nluc-PAK1 were assayed for luciferase activity according to the manufacturer’s Nano-Glo® Live Cell Assay System protocol (Promega). In brief ...
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bioRxiv - Immunology 2022Quote: ... followed by a PBS wash and lysis buffer to measure the luciferase activity in cell lysates using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Physiology 2019Quote: The expression profile of PKC isoforms in UMR106 cells was studied by RT-PCR using the GoTaq Green Master Mix (Promega) and the primers listed in Table 1 ...
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bioRxiv - Biochemistry 2019Quote: A commercial K562 cell digest supplemented with the 6 x 5 LC-MS/MS Peptide Reference Mix (QC4Life Reference Standard, Promega) was used as quality control (QC ...
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bioRxiv - Biochemistry 2019Quote: ... Both human cell lines were validated using the cell line authentication service at Duke University and confirmed using the GenePrint 10 kit from Promega and tested to be mycoplasma-free ...
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bioRxiv - Biophysics 2020Quote: ... The cells were co-transfected with the plasmid carrying the Rh-PDE variant and the pGloSensor-22F cAMP vector (Promega), by using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... An equal amount of BacTiter-Glo reagent was added to each well as described by the manufacturer (BacTiter-Glo Microbial Cell Viability, Promega). For ATP quantification under normoxia ...
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bioRxiv - Cell Biology 2019Quote: ... The cells with successful incorporation in the genomic locus of EGFP or TagRFP were screened by PCR using GoTaq Polymerase (Promega).
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bioRxiv - Cell Biology 2020Quote: Measurements of caspase activities in cells were performed using the commercially available Caspase-Glo 3/7 Assay (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... or R602P) and Cbs-pCMV-sport6 (either wild type or I278T) were transfected into the cells with Fugene HD (Promega). Cells were incubated for 48 hours and then DMEM was removed ...
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bioRxiv - Genomics 2019Quote: ... The reporter plasmids and β-gal expression plasmids were cotransfected in K562 cells and luciferase assay was carried out after 48 hours using Nanoglo luciferase kit (Promega) and normalized to ß-gal expression measured using assay reagent (ThermoFisher).
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bioRxiv - Cell Biology 2019Quote: Approximately 5 × 107 control cells and 2 × 107 sort3 PIGS-KO HEK293 cells were used for genomic DNA extraction by Wizard Genomic DNA Purification Kit (Promega). Approximately 325 µg of genomic DNA from control cells and 30 µg of genomic DNA from sort3 cells were used for amplification of gRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed 48 hours after transfection and assayed for luciferase activity using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions using a Flexstation 3 Multi-mode Microplate Reader (Molecular Devices) ...
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bioRxiv - Cancer Biology 2020Quote: ... viruses were generated in HEK 293T cells transfected with lentiviral packaging vectors along with vectors expressing pGIPZ-shRNA using Fugene6 (Promega). Two distinct hairpins were chosen for the experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... RL and FL activities were measured on 96-well plates using 2 µl of cell extract containing 8 µl of 1x PLB (Promega) and the dual-luciferase assay kit (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were collected in PBS and luciferase activity was assessed using the Nano-Glo Dual Luciferase reporter assay system (Promega). Dual luciferase signal was quantified using a VICTOR-3 plate reader (Perkin Elmer) ...
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bioRxiv - Microbiology 2020Quote: ... The former cell lysates were subjected to a luciferase assay to normalize the transfection efficiency using a firefly Luciferase Assay System (Promega) with a Centro LB960 luminometer (Berthold) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were harvested 36 h after transfection by lysing in 100 μl of 1× reporter lysis buffer (Promega, Madison, WI). Lysates were pelleted ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested 24 h after treatment and luciferase activity was measured using Luciferase Assay System (Promega, Leiden, The Netherlands). Infections were performed using pseudotyped particles obtained by co-transfecting HXB2 Env with pNL4.3.Luc.R-E-plasmid into HEK 293T cells using PEI ...
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bioRxiv - Cancer Biology 2019Quote: ... and cells were lysed in a mixture of 50 μL media and 50 μL CellTiter-Glo 2.0 reagent (Promega # G9243) per well ...
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bioRxiv - Microbiology 2019Quote: ... differences in cell viability due to virus-induced cytopathic effect (CPE) or compound-specific side effects were analyzed using the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (Promega), as described previously (de Wilde et al. ...
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bioRxiv - Physiology 2020Quote: ... Cells were harvested 6 h after stimulation and luciferase activity was measured according to the manufacturer’s protocol (Promega, Madison, WI). Relative luciferase activity was normalized to the β-galactosidase activity measured using multi-mode plate reader (SpectraMax M5 ...
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bioRxiv - Immunology 2020Quote: ... The activity of Renilla luciferase in total cell lysates was measured with the use of a Dual-Luciferase Reporter Assay System (Promega) and was normalized by that of firefly luciferase derived from 80 ng of a control plasmid.
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bioRxiv - Cell Biology 2021Quote: ... Fresh batches of ARPE-19 cells from a contamination-free stock that had been tested for mycoplasma (MycoAlert; Promega Inc.) were used every 6 to 8 weeks ...
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bioRxiv - Microbiology 2020Quote: ... towards HeLa cells was determined as a release of the intracellular enzyme lactate dehydrogenase (LDH) into the cell culture media using CytoTox 96 assay (cat.no. G1780, Promega, USA) or as changes in cell membrane integrity using CellTox Green Cytotoxicity Assay (cat.no ...
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bioRxiv - Cell Biology 2020Quote: IC50 was determined on days 3 and 4 after vemurafenib treatment using Real-time-Glo MT Cell Viability Assay according to the manufacturer’s protocol (Promega, #G9711). Using a 96-well white plate ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were diluted and aliquoted to 96-well plates to make less than 1 cell count per well and lysed by 30 μl of Passive Lysis Buffer (Promega) when confluent ...
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells [41] for 18 hours at 37°C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were incubated for 3 days at 35°C with 5% CO2 and cell viability was evaluated using the CellTiter Glo (Promega) post the 3-day incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... media in each well was replaced with 70 μl fresh medium 70 μl of Cell Titer-Glo reagent (Promega #G7570) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell proliferation was measured every day for up to 72 hours with the CellTiter-Blue assay (#G8080, Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... In 18 hours cells were stimulated for 8 hours with a corresponding inducer and harvested in the passive lysis buffer (Promega). Luminescence of Firefly and Renilla luciferases was measured using dual-luciferase-reporter assay (Promega ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed once with PBS and their viability was measured with a kit designed to quantify ATP level according to instructions of the manufacturer (CellTiterGlo Luminescent Cell Viability Assay, Promega).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 40 μl of the cell suspension were transferred to each well of 96-well plates containing 10 μl of 5X GloSensor cAMP Reagent (Promega) prepared according to the manufacturer’s instruction ...