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Citations for Promega :
5051 - 5100 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Cell viability and in vitro luciferase expression was evaluated via ONE-Glo + Tox luciferase reporter and cell viability assay kit (Promega) and a multimode microplate reader.
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bioRxiv - Cancer Biology 2024Quote: ... The PC-3 cell line was authenticated by Macrogen (Korea) using short tandem repeat (STR) profiling (Powerplex 21 System, Promega). All cell lines tested negative for mycoplasma contamination using the Microsart AMP Mycoplasma Kit (Sartorius).
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bioRxiv - Cell Biology 2024Quote: pSBtet-BP plasmids encoding various AAK1 WT and AAK1 mutant constructs alongside pCMV(CAT)T7-SB100 were co-transfected into ARPE-19 cells using FuGene HD transfection reagent (Promega), as per manufacturer’s protocol (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then lysed 24 hours after treatment and luciferase activity was measured using the Dual Luciferase Assay (E1910, Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested after 24 h and luciferase activity was measured using the Luciferase Reporter Assay System (Promega, Cat. #E1500) on Victor Nivo luminometer (PerkinElmer) ...
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bioRxiv - Genetics 2024Quote: ... We prepared triplicate tissue homogenates from ten F1 progeny from each cross using the Luciferase Cell Culture Lysis 5X Reagent (Promega) to extract total proteins by following the quick-freeze homogenization method outlined by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... and then cells were transfected with the fragments which were cloned into the pGL4.10 luciferase reporter basic vector (E1751, Promega, USA), and the PRL-TK reilla luciferase reporter constructs were used as an internal control for plasmid transfection efficiency ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Molecular Biology 2024Quote: ... the amount of fluorescence of supernatant and solubilized cells was measured with a Glomax multi + detection system (Promega, WI, USA). The efflux capacity was calculated from the ratio of fluorescent intensity in the supernatant and the total fluorescent intensity from the supernatant and cell lysate ...
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bioRxiv - Microbiology 2024Quote: ... cells were infected at MOI 1 for 24 h and cells were frozen dry at -80°C for 30 min and lysed in Passive Lysis Buffer (Promega). Bioluminescence was measured using the Nano-Glo® Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2024Quote: ... SL100688) with the exception of BCBL-1 cells that were transfected with FuGENE HD transfection reagent (Promega, Cat. No. E2311,). Competition reporter assays were performed with co- transfection of the TR7 reporter plasmid with or without LANA expression plasmid (DY52) ...
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bioRxiv - Immunology 2024Quote: ... Cells were then collected and washed before cell lysis and addition of luciferin substrate from the Bright-Glo Luciferase Assay System (Promega). The resulting luminescent signal was measured using a Tecan Infinite M200 Pro ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in native lysis buffer and luciferase activity was assayed using the Dual-Luciferase Reporter Assay (E1910, Promega) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... the cells were collected and lysed for the reporter luciferase activities measured by using a dual-luciferase reporter assay system (Promega). All the luciferase activity values were gained against the Renilla luciferase control ...
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bioRxiv - Microbiology 2024Quote: ... ATP-depleted uRBCs were then washed three times with PBS and ATP-depletion was evaluated for each dilution using the CellTiter-Glo→ Luminescent Cell Viability assay (Promega) following the instructions provided in the kit (see Extended Data Fig ...
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bioRxiv - Microbiology 2023Quote: ... Fresh MEM supplemented with 200 μM GCDCA was then added on the cells in the presence or absence of Z-VAD-FMK (Promega), NH4Cl (Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of purified bacmid DNA were transfected into 1×106 Sf9 cells in a well of a 6-well plate using 6 μL of FuGENE HD transfection reagent (Promega). After 7 days of incubation at 27°C ...
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bioRxiv - Bioengineering 2023Quote: ... A pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells with the pHR transgene expression vector and viral packaging plasmids pCMVdR8.91 and pMD2.G using Fugene HD (Promega #E2312). At 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell line authentication was performed by short tandem repeat DNA analysis with the GenePrint 10 System (Promega, Fitchburg, WI, USA).
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bioRxiv - Synthetic Biology 2023Quote: DNA (genomic DNA pool and plasmid DNA pool) was isolated from the cell library using Wizard SV DNA purification system (Promega) for determining genome engineering efficiencies ...
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bioRxiv - Genetics 2023Quote: ... Luciferase activity in cell lysates was assayed 48 hours post-transfection using the Dual Luciferase Reporter Assay kit (Promega E1910), and the Fluoroskan Ascent FL microplate reader (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then lysed and Firefly and Renilla luciferase activities were determined with the Dual-Luciferase Reporter Assay System (Promega) and an EnVision plate reader (PerkinElmer ...
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bioRxiv - Cell Biology 2024Quote: ... RNA from RHE was extracted using the “ReliaPrep™ miRNA Cell and Tissue Miniprep System” (Promega Cat#Z6111, Wisconsin, USA) and its concentration measured by NanoDrop 1,000 spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of inhibitor and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Luciferase activity was determined 18-24 hr post-transfection from cell lysate using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturers protocols ...
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bioRxiv - Immunology 2023Quote: ... 105 cells per target population were sorted and kept on ice until further processing using GSH-GloTM Glutathione Assay (Promega), strictly following manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of protease inhibitors and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the luciferase activity in cells was analyzed using a GloMax 20/20 luminometer with the Dual-Luciferase Reporter Assay system (Promega).
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bioRxiv - Microbiology 2022Quote: ... 48 h post transfection cells were harvested for western blotting or luminescence detection using a Firefly Luciferase Assay kit (Promega) and a Glomax luminometer (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... Caspase 3/7 enzymatic activity in raw cell lysates was measured using a Caspase Glo 3/7 assay kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Gaussia luciferase activity in the supernatants was measured by addition of Coelenterazine (PJK Biotech) and firefly luciferase activity was measured in the cells using the Luciferase Assay System (Promega) according to the manufacturer’s instruction.
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bioRxiv - Genomics 2023Quote: ... cells were incubated with DSPE liposomes at concentrations of 0-5mg/ml in DMEM containing the viability dye from the RealTime-Glo MT Cell Viability Assay (cat: G9712, Promega). After incubation with liposomes for 4h ...
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bioRxiv - Cell Biology 2023Quote: ... was obtained through transfecting HEK293T cells with these plasmids in combination with a second generation CMV lentiviral packaging system and FuGENE transfection agent (E2311, Promega).
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bioRxiv - Immunology 2023Quote: The same 96 well setup was used as for the PBMC assay with 50000 T2 and 50000 Jurkat NFAT-Luciferase cells per well (Promega) in 100ul R10 medium ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 μL of Nano-Glo HiBiT Lytic Detection Reagent was added directly to the cells and incubated for 5 min on an orbital shaker (300 rpm) before recording luminescence on a GloMax Discover (Promega) with 0.2 s integration time.
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bioRxiv - Neuroscience 2023Quote: ... We performed targeted PCR by adding 1 μL of cell lysate (1:5 dilution) to a 25-μL PCR reaction containing GoTaq Hot Start Master Mix Green (Promega) and 0.5 μL of the primers (10 µM ...
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bioRxiv - Microbiology 2023Quote: ... cells grown on white 96-well plates with clear bottoms were transfected with a polio replicon RNA expressing the Renilla luciferase gene in the complete medium supplemented with 30 μM of cell-permeable Renilla luciferase substrate Enduren (Promega). The plates were sealed with a transparent oversheet and incubated directly in the heated chamber of a multifunctional M3 microplate reader (Molecular Devices) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were maintained at 37 °C and 5% CO2.Cell lines were authenticated using Short Tandem Repeat fingerprinting carried out using the GenePrint 10 system (Promega). For 3D spheroids ...
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bioRxiv - Cell Biology 2023Quote: ... Annealed gRNA oligonucleotides were inserted into vector px458 and the gRNA construct was transfected into HeLa cells using ViaFect (Promega) transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed after exactly 24 hours of stimulation in 1X Passive Lysis Buffer (100 μl per well (Promega, #E1941)) ...
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bioRxiv - Cell Biology 2023Quote: ... Other parameters of ER Stress induced cell death were measured through immunoblotting or with the Caspase 3/7 Glo Assay Kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cardiomyocyte viability was measured as changes in cellular ATP levels using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega). Cardiomyocytes cultured for 20 h were stimulated with CNP (1 µM) ...
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bioRxiv - Biophysics 2023Quote: Schneider S2R+ cells on coverslips were transfected with GFP-LKB1 variants under a ubiquitous promoter (Ubi::GFP-LKB1) using FUGENE (Promega).35 48 h after transfection ...
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bioRxiv - Developmental Biology 2023Quote: Protein extracted from 10 CNC explants as described above or immunoprecipitated with the Flag antibody from embryos or Hek293T cells were diluted in 8M urea and processed with Trypsin/Lysine-C according to manufacturer instruction (Promega). Tandem mass spectrometry was performed using a Thermo orbitrap Fusion (Mass spectral data were obtained at the University of Massachusetts Mass Spectrometry Core Facility ...
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bioRxiv - Cancer Biology 2023Quote: ... and 500 ng of each of the packaging plasmids were transfected into 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells in a 6-cm dish were transiently transfected with 1 μg of pGloSensor-22F cAMP plasmid (Promega, USA) and 1 μg of human wild-type or mutated A2AR or A2BR overexpression plasmid using polyethyleneglycol (6 μl ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation of DHX15-G4-FL and DHX15-G4-Del-FL transcripts in the presence of F1 or BRACO19 was carried out in a cell-free translation system of rabbit reticulocyte lysates (Promega) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The firefly luciferase and Renilla luciferase activity in each cell were detected by dual-luciferase reporter assay system (Promega, E1910) and the relative luciferase activity was further normalized to Renilla luciferase activity.
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with act5c-Gal4 plasmid (600 ng) and pUAST-based expression plasmid (1000 ng) using FugeneHD reagent (Promega). Transfected cells were cultured at 25°C and processed within 1 to 3 days.