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Citations for Promega :
4951 - 5000 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Parasites viability was assessed by direct light microscope observations and MTS colorimetric assays (CellTiter Aqueous One Solution Cell Proliferation Assay -Promega); they maintained shape and motility at 5 ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 VLPs were produced by transfecting 2×106 293T-wt cells in T75 flasks with 25 μg total DNA using Fugene6 (Promega). The codon optimised plasmids expressing SARS-CoV-2 M ...
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bioRxiv - Microbiology 2021Quote: ... The cytotoxicity of compounds (100 uM to 1 uM, 3-fold dilution) was examined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cell cytotoxicity data was normalized to DMSO control as 0% cell death.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... retrovirus vector containing chick U6 promoter which expresses shRNA for gfp or Plzf was transfected into M/O chicken strain-derived embryonic fibroblast cells (CEF) cultured in 60-mm dish using Fugene HD transfection regent (Promega). CEF was cultured in DMEM-high glucose containing 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... the cells were lysed and luciferase activity was measured in the lysates using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLU ...
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bioRxiv - Genetics 2021Quote: ... Cell death was analyzed similarly using a commercially available kit that labels fragmented DNA from apoptotic cells (DeadEnd Fluorometric TUNEL System, Promega).
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bioRxiv - Neuroscience 2019Quote: Receptor responses were measured as luminescence of Firefly and Renilla reporters in a cell based assay following the protocol for the Dual-Glo Luciferase Assay System (Promega) described in (24 ...
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bioRxiv - Synthetic Biology 2020Quote: Cells from HAC-containing clones have been harvested and genomic DNA has been collected using Maxwell DNA purification kit (Promega). qPCR analysis have been performed using SYBR Green Master Mix (Roche ...
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bioRxiv - Physiology 2019Quote: ... cells were harvested for the determination of Firefly and Renilla luciferase activity using the dual-luciferase reporter assay system (Promega), and Firefly luciferase activity was normalized to Renilla luciferase activity ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then transiently-transfected with 100 ng/well cDNA encoding NLuc/NRP1 or NLuc/VEGFR2 using FuGENE (Promega, USA) according to the manufacturer’s instructions and incubated for a further 24h at 37°C/5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation was measured by a MTS conversion assay using the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation kit (#G5421, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Trpc4 and Trpc5 mRNAs was performed on 3 µl of cDNA from single cells in a 30 µl reaction volume containing 1X GoTaq Flexi buffer (Promega), 2 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 50 μL (fLuc assays) or 100 μL (NanoLuc assays) of 1× Cell Culture Lysis Reagent (Luciferase Assay System; Promega) and incubated for 10 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... 2 µg NFIA expression construct (NFIA-FLAG) was transfected to each well of cells by FuGENE HD Transfection Reagent (Promega). Nuclear extracts containing the NFIA protein were extracted from 3 wells of transfected cells using nuclear and cytoplasmic extraction kit (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... HF and MEF cell lines were transfected with 10 ng of the internal control plasmid pTK-Renilla (pRL-TK, Promega) or 60 ng of the reporter plasmid pLUC-NF-κB (R ...
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bioRxiv - Microbiology 2021Quote: ... An MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]-based viability assay (CellTiter 96 aqueous nonradioactive cell proliferation assay, Promega) was performed as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested in passive lysis buffer and luciferase assay was performed using Dual-Luciferase Assay System (Catalogue # E1960, Promega), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: HEK cells were seeded at 3.0 × 106 cells in 10 cm dishes and transfected with 3 μg of the indicated DNA construct using FuGENE HD (Promega). 24 h post-transfection ...
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bioRxiv - Biophysics 2019Quote: ... coli HB101 cells and isolated in quantity by using a Promega Wizard Plus Megaprep purification kit (Promega Corp., Madison, WI).
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bioRxiv - Neuroscience 2019Quote: ... The samples were then cleared by centrifugation and the supernatant was diluted (1/50) with extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). The luminescence was measured on an Infinite M200PRO plate reader (TECAN ...
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bioRxiv - Molecular Biology 2019Quote: ... The identity of all cell lines was confirmed by DNA finger printing analysis utilizing the GenePrint® 10 System (Promega). BT-474 and MDA-MB-453 cells were cultivated in DMEM medium (Pan-Biotech ...
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bioRxiv - Biochemistry 2019Quote: ... aliquot of approximately 107 cells were resuspended in 100 μL of lysis buffer (0.1 % w/v ProteaseMAX Surfactant (Promega, USA) in 50 mM ammonium bicarbonate and 10 % v/v ACN) ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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bioRxiv - Genomics 2021Quote: ... Cells from each cell line were cultured for an additional 48 hours until total RNA isolation (Maxwell RSC simplyRNA kit, Promega).
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bioRxiv - Genetics 2020Quote: ... Cell line identities were regularly confirmed by short tandem repeat (STR) profiling using the PowerPlex16 system and GeneMapper software (Promega)27 ...
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bioRxiv - Genetics 2020Quote: The frameshifting efficiency of the reporter plasmids in cultured cells were assayed as described previously (15, 16) using a dual luciferase reporter assay system kit (Promega). 24 hours post transfection ...
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bioRxiv - Immunology 2021Quote: Toxicity was measured by quantifying the cell viability using the CytoTox-ONE™ Homogeneous Membrane Integrity Assay kit (Promega, G7891) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... was synthesised in 20 µl reaction volumes with 500 ng (ISGs) or 100 ng (PIV5-infected cells) total RNA and oligo(dT) using GoScript reverse transcriptase (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... Firefly (Fluc)- and Renilla (Rluc)-luciferase activity in each cell lysate was determined using the Dual Luciferase Assay Kit (Promega) and a Berthold tube luminometer ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were transfected with 500 ng of 3xFLAG or 3xFLAG-PARP1 plasmids using Fugene HD transfection reagent (Promega, # E2311) according to manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: Cells were seeded at a concentration of 3×105/mL in RPMI medium on day 0 and the number of cells was measured every two-three days using the MTS assay from Promega following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and 24 hours later cells were lysed with Passive Lysis 1x and reporter activities measured with Dual Luciferase Reporter Kit (Promega). At the same time supernatants from p1 cells were cleared by centrifugation and passaged onto fresh HEK293T-TIM1 p2 target cells ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hours the cells were washed briefly with 1x PBS prior to measurement of luciferase activity by Dual Luciferase Reporter Assay (Promega).
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bioRxiv - Cell Biology 2019Quote: ... Approximately 100bp of the surrounding sequence was amplified by PCR from LX2 cells and cloned into the XhoI site of the pGL3+promoter vector (Promega) with corresponding empty vector as control ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids with the ATAD1 variants under a truncated CMVd3 promoter were transfected into cells using the FuGENE HD transfection reagent (Promega), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: The LC50 values for the drugs (MTX, CPT and TDX) were determined by CellTiter-Blue® cell viability assay (Promega). Cells were seeded into 96 plates at a density of 3000 cells per well ...
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bioRxiv - Molecular Biology 2021Quote: ... the transfer plasmids of interest were co-transfected with GAG/POL and VSVG plasmids into 293FT cells using FuGENE HD (Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were transfected with Halo-FLAG-FUS plasmids using the FuGENE HD Transfection Reagent (Promega, Madison, WI, catalog #: E2311). The cells were cultured in a medium containing the ligand (10 μM ...
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bioRxiv - Biochemistry 2020Quote: ... the culture medium was aspirated and 20 μL of cell lysate was added from the Luciferase Assay System (Promega, E1500) to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was suspended in buffer P1/RNAse A and lysed by adding 1/20 volume of 10% SDS (Promega). After incubating for 20 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell viability for 3 biological replicates of each treatment condition was assessed after 72 hours after drug treatment using the CellTiter-Glo Luminescent Cell Viability Assay (G7571, Promega) and an EnVision Multilabel Plate Reader (PerkinElmer) ...
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bioRxiv - Cell Biology 2019Quote: ... Hana 3A cells were authenticated using polymorphic short tandem repeat (STR) at the Duke DNA Analysis Facility using GenePrint 10 (Promega) and shown to share profiles with the reference (ATCC) ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-NPHP2 and GFP-NPHP5 expression vectors were transiently transfected to 293T/17 cells together with pCS2FLAG empty vectors (as a control) or pCS2FLAG-NPHP1 variants using FuGENE HD (Promega), following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then transfected with each reporter construct (450ng) and the 50ng pGL4.74 Renilla luciferase expression vector (Promega; Madison, WI) using ViaFect Transfection Reagent (Promega ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted from cells using the ReliaPrep™ RNA Miniprep System kit in accordance with the manufacturer’s protocol (Promega). RNA was reverse transcribed using the Applied Biosystems™ high capacity RNA to cDNA™ Kit in accordance with the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded in triplo in opaque 96-well plates and cell viability was measured 96h later using the GloMax Discover Microplate reader (Promega). Luciferase reporter assay (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... the MCF-7 and MDA-MB-231 cells were infected with Plasmids expressing RFP or GFP using Fugene 6 (Promega) at an early passage and were selected using 2 μg/ml puromycin (Sigma).
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bioRxiv - Cancer Biology 2021Quote: ... Retrovirus was generated by transfecting SETBP1 pMSCV-IRES-mCherry or CSF3R pMSCV-IRES-GFP or control vector plasmids and the EcoPac plasmid (provided by Dr. Rick Van Etten) into the 293T17 cells using FuGENE 6 (Promega). Viral supernatants were harvested 48 and 72 hours later ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins from control HeLa cells with C-terminal mEGFP tag were digested with mass-spectrometry grade Lys-C and trypsin (Promega). Eluted proteins from a cell line lacking full-length Cdc20 (CDC20_M1-fs-M43 ...
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bioRxiv - Genomics 2021Quote: 293T cells were co-transfected with plasmid DNAs of a retroviral vector and the helper vectors using FuGENE HD (Promega). Medium was collected 24 ...