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Citations for Promega :
5101 - 5150 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Then after another 24 hr cells were lysed and luciferase activity was measured by using the luciferase assay system (Cat no E4030) from Promega.
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bioRxiv - Biochemistry 2023Quote: ... 1.5 µg of the pAC8RedNK vector and 0.5 µg of linearized baculoviral DNA were concurrently transfected into 1×106 Sf9 cells using 6 µl of FuGene HD (Promega) in ESF 921 Insect Cell Culture medium (Expression Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 500 ng of each of the packaging plasmids were transfected into 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with act5c-Gal4 plasmid (600 ng) and pUAST-based expression plasmid (1000 ng) using FugeneHD reagent (Promega). Transfected cells were cultured at 25°C and processed within 1 to 3 days.
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bioRxiv - Cancer Biology 2023Quote: ... The Genetic Resources Core Facility at the John Hopkins University School of Medicine authenticated the HPAF-II cells by performing short tandem repeat profiling via GenePrint 10 (Promega) and comparing to the ATCC database ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells in a 6-cm dish were transiently transfected with 1 μg of pGloSensor-22F cAMP plasmid (Promega, USA) and 1 μg of human wild-type or mutated A2AR or A2BR overexpression plasmid using polyethyleneglycol (6 μl ...
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bioRxiv - Cancer Biology 2023Quote: ... The firefly luciferase and Renilla luciferase activity in each cell were detected by dual-luciferase reporter assay system (Promega, E1910) and the relative luciferase activity was further normalized to Renilla luciferase activity.
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bioRxiv - Developmental Biology 2023Quote: Protein extracted from 10 CNC explants as described above or immunoprecipitated with the Flag antibody from embryos or Hek293T cells were diluted in 8M urea and processed with Trypsin/Lysine-C according to manufacturer instruction (Promega). Tandem mass spectrometry was performed using a Thermo orbitrap Fusion (Mass spectral data were obtained at the University of Massachusetts Mass Spectrometry Core Facility ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation of DHX15-G4-FL and DHX15-G4-Del-FL transcripts in the presence of F1 or BRACO19 was carried out in a cell-free translation system of rabbit reticulocyte lysates (Promega) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... After removal of supernatant cells were resuspended in 100 ul of freshly prepared 1X Renilla Luciferase Assay Lysis buffer (Promega). Samples were analyzed with the Renilla Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2023Quote: Expression vectors encoding Foxp3 and Ikzf1 and/or Ikzf1 mutants were transfected into HEK293T cells (2 × 105) with FuGENE HD (Promega). 48h after transfection ...
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bioRxiv - Immunology 2023Quote: ... Retrovirus was generated by transfecting the Plat-E packaging cell line with 10 µg plasmid DNA and 30 µl FuGene HD transfection reagent (Promega). Viral supernatants were collected from transfected Plat-E cells at 40 ...
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bioRxiv - Cell Biology 2023Quote: ... S2R+ cells were grown in SF-4 medium and cotransfected with desired plasmids using FuGENE® HD transfection reagent (Promega). Specifically ...
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bioRxiv - Cancer Biology 2023Quote: ... Testing was performed in a 96-well plate-format with 10,000 cells per well and using dose-response matrices in a 10×10 grid manner (0-10uM in two-fold dilutions) and cell viability was measured with CellTiter-Glo (Promega). The dose-response matrices were then used to compute synergy and antagonism scores using Bliss synergy modelling with Combenefit software.
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bioRxiv - Cancer Biology 2023Quote: ... Ten thousand cells per well were treated in a 96-well plate-format for 72hrs with a range of concentrations (0-50uM in a series of 20 two-fold dilutions) and cell viability was measured with CellTiter-Glo (Promega). Drug sensitivity was compared by calculating IC50 values between MM cell lines using four parameter non-linear regression models and one-way ANOVA analysis for multiple comparisons ...
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bioRxiv - Biochemistry 2023Quote: ... and plates were either run in the XF Analyzer or used to measure intracellular ATP concentration with the Cell-Titer Glo Assay (Promega). ATP measurements were taken before and after activation with magnetic Dynabeads coated with anti-CD3/CD28 antibodies (Thermofisher ...
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bioRxiv - Biochemistry 2023Quote: ... stable HEK293T cells expressing wild type COMT were transiently transfected with pTagBFP-C expressing the KDEL marker using FugeneHD (Promega) following the manufacturer’s instructions with 100 ng plasmid ...
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bioRxiv - Microbiology 2023Quote: Viral stocks were generated in tissue culture by transfecting MCMV genome-carrying BACs into NIH3T3 cells (ATCC CRL-1658) using FuGene HD (Promega). Reconstituted viral particles were passaged five times on M2-10B4 cells (ATCC CRL-1972 ...
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bioRxiv - Bioengineering 2023Quote: ... tumor fragments (200-300 mg) were lysed for 1 h on ice with agitation in 1X Reporter Cell Lysis Buffer (Promega) and then centrifuged at 14,000 x g for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... shRNA-expressing lentiviruses were generated by co-transfecting 293T cells with pLKO-puro.1 vectors and the packaging vectors ΔVPR and VSVG (FuGENE, Promega). Viruses were harvested 24-48 h post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 5×104 cells/well were seeded in 24-well plates and transfected with 10 ng pRL-CMV (Promega, Walldorf, Germany) together with either 250 ng pSuper8xTOPFlash or 250 ng pSuper8xFOPFlash [22] using the FuGENE6® reagent (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed 24 h after transfection and luciferase activity was measured with Dual-Luciferase Reporter Assay (Promega Cat. # E1960) according to the manufacturer’s protocol using a FlUOstar plate reader (BMG Labtech) ...
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bioRxiv - Genomics 2023Quote: DNA was extracted from the agar/cell mixture from the output and control pools using a Wizard Genomic DNA Purification Kit (Promega). The manufacturer’s protocol was followed with two modifications ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with packaging vectors pMD2.G and psPAX2 along with relevant shRNA using Fugene 6 transfection reagent (Promega). Viral particles were harvested after 48 hours in DEMEM/ 40% FBS ...
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bioRxiv - Immunology 2023Quote: The purification of total RNA and reverse transcription to synthesize cDNA were performed using a ReliaPrep RNA Cell Miniprep System (Promega) and ReverTra Ace qPCR RT Master Mix (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... 8 × 105 HEK293T cells/well were seeded into 6-well plates and transfected the next day with ViaFect (Promega, E4981), using either 4 μg of plasmid expressing the G protein or 2 μg of plasmid expressing HA together with 2 μg of plasmid expressing NA ...
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bioRxiv - Cancer Biology 2023Quote: ... we transfected 500,000 ANBL6 cells with 1 ug of reporter plasmid plus 250 ng of Rinella plasmids pRL-SV40 and pGL3 control (Promega) in biological triplicates ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was determined by measuring the cellular ATP levels using CellTiter-Glo reagent (CellTiter-Glo® 2.0 Assay, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were harvested and the activities of firefly and Renilla luciferase were measured in the same cell lysate using the Dual-Luciferase Reporter Assay System (Promega) and GloMax Luminometer (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with 1× PBS and lysed with PLB according to the manufacturer’s instruction for 15 mins (Promega, E1910). Cells were transferred to a 96-well plate ...
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bioRxiv - Cancer Biology 2023Quote: The activity of EBV miRs-BART 7-3p and 9-3p was evaluated in Akata-EBV/Cas9 cells by luciferase reporter gene assay with constructs based on the psiCheck-2 backbone (Promega). 3’-UTR sequences with miRNA-binding sites (Supplementary Material ...
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bioRxiv - Immunology 2022Quote: ... 5×106 HEK293 cells were plated in 15 cm petri dishes and transfected the day after with 50 μL FuGENE (E2311, Promega), 10 μg CAR-encoding vectors and 3.3 μg of each 3rd generation lentivirus helper vectors (CART-027CL ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were maintained at 37 °C and 5% CO2.Cell lines were authenticated using Short Tandem Repeat fingerprinting carried out using the GenePrint 10 system (Promega). For 3D spheroids ...
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bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: Schneider S2R+ cells on coverslips were transfected with GFP-LKB1 variants under a ubiquitous promoter (Ubi::GFP-LKB1) using FUGENE (Promega).35 48 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Annealed gRNA oligonucleotides were inserted into vector px458 and the gRNA construct was transfected into HeLa cells using ViaFect (Promega) transfection reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell line authentication was performed by short tandem repeat DNA analysis with the GenePrint 10 System (Promega, Fitchburg, WI, USA).
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 24hr at 37°C and cell death was measured through a lactate dehydrogenase (LDH) assay using the CytoTox 96 kit (Promega). 50 μL of media was collected from all experimental wells with two replicates each and placed in a fresh 96-well plate ...
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bioRxiv - Bioengineering 2023Quote: ... A pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells with the pHR transgene expression vector and viral packaging plasmids pCMVdR8.91 and pMD2.G using Fugene HD (Promega #E2312). At 48 hours ...
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bioRxiv - Synthetic Biology 2023Quote: DNA (genomic DNA pool and plasmid DNA pool) was isolated from the cell library using Wizard SV DNA purification system (Promega) for determining genome engineering efficiencies ...
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bioRxiv - Physiology 2023Quote: In vitro cytotoxicity was determined by measuring lactate dehydrogenase (LDH) release upon cell lysis according to the manufacturer’s instructions (Promega, G1780). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 4% FBS and 100 µL were seeded per well in 96-well plates at a density of 2×105 cells/mL in the presence or absence of 0.1 mM HaloTag NanoBRET™ ligand (Promega). The following morning ...
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bioRxiv - Genetics 2023Quote: ... Luciferase activity in cell lysates was assayed 48 hours post-transfection using the Dual Luciferase Reporter Assay kit (Promega E1910), and the Fluoroskan Ascent FL microplate reader (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then lysed and Firefly and Renilla luciferase activities were determined with the Dual-Luciferase Reporter Assay System (Promega) and an EnVision plate reader (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: The intracellular ATP content of bacteria suspended in mineral water was determined using the luciferase-based BacTiter-Glo Microbial Cell Viability Assay kit (Promega). As per the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... The cell culture supernatant was collected and subjected to analysis using the CytoTox 96 assay (Promega, Madison, WI, USA; G1780) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... We also assessed cell viability of each sample in triplicates through a luminescence assay of ATP (CellTiter-Glo 2.0 Assay, Promega #G9242) following manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... LDH activity in the supernatants and the cell lysates was then assessed using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) by following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was generated in HEK293T cells by transfecting lentiviral transfer vector, and helper vectors (psPAX2, and pVSV-G) using Fugene HD (Promega). Lentiviral supernatant was collected 48 hours after transfection ...