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Citations for Promega :
4751 - 4800 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Cells were plated in black 96-well plates (Grenier Bio One) and furimazine (Promega, Nano-Glo Luciferase Assay System) was added to each well ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were plated in a 24-well plate at 90,000 cells per well and transfected with gRNAs using Fugene HD (Promega) at a manufacturer-suggested 3:1 reagent:DNA ratio ...
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bioRxiv - Genomics 2024Quote: ... and psPAX2 was used for packaging in 293T cells using FuGENE® HD Transfection Reagent (Promega, cat no. E2311). Lentivirus was concentrated using Lenti-X Concentrator (Takara Bio cat no ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were was lysed and Cell lysates were subjected to dual reporter luciferase assays according to the manufacturer’s instructions (Promega).
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bioRxiv - Microbiology 2024Quote: ... Cell culture media was replaced by PBS prior to photon emission measurement using a Glomax Discover microplate reader (Promega). Cells were lysed in distilled water for intracellular bacterial counts on LB agar as described in (Kühbacher et al. ...
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bioRxiv - Microbiology 2024Quote: ... Luminescence (relative luminesce units, RLU) and Absorbance (OD595) of sample triplicates supplemented with NanoGlo Live Cell Assay System (Promega) as recommended by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... A T cell’s response to antigen was measured as luciferase activity using the Nano-Glo Luciferase assay system (Promega) according to the manufacturer’s instructions (as above) ...
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bioRxiv - Microbiology 2024Quote: ... Twenty-four hours after transfection cells were harvested for luciferase assay using the Dual-Glo Reporter Assay Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: NADP(H) content in the yeast cells was assessed with NADP/NADPH-Glo Assay according to the manufacturer’s protocol (Promega) with the modification described by Kwolek-Mirek et al ...
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bioRxiv - Genetics 2024Quote: ... 24 hours later cells were transfected with 50ng regulatory element plasmid and 50ng Renilla plasmid (pGL 4.74; Promega, E6921) using 0.3µL Fugene HD (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... and GSSG levels) was determined in the yeast cells with GSH/GSSG-Glo Assay according to the manufacturer’s protocol (Promega) adapted for yeast cells as was done by Kwolek-Mirek et al ...
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were harvested for RNA extraction with a Maxwell RSV Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293TN cells were seeded in 6-well plates and transiently transfected with plasmid of choice using FuGene transfection reagent (Promega) and OptiMem (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... viruses were generated in HEK293T cells transfected with lentiviral packaging vectors along with vectors expressing pGIPZ-shSTEAP3 using Fugene6 (Promega). Two distinct hairpins were chosen for the experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... The 96-well plate was then allowed to equilibrate at room temperature for 30 minutes followed by addition of Cell Titer Glo (Promega) to each well according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... samples corresponding to 4.44×105 cell equivalents of the translation reaction were put on ice and mixed with 1x Renilla-Glo substrate (Promega) in Renilla-Glo (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated in a cell culture incubator with 5% carbon dioxide at 37 degrees Celcius for 72 hours before cells were washed twice with PBS then measured via CellTiter-Glo (Promega). IC50 experiments were repeated three times for each cell line shown.
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bioRxiv - Cell Biology 2020Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). Luminescence was measured with a SpectraMax Gemini XPS luminometer (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed in the lysis buffer that was included in the Dual-Luciferase® Reporter Assay System (Promega, E1910). To the cell lysate ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then cleared by centrifugation and the supernatant was diluted (1/50) with extraction buffer and mixed with luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). The luminescence was measured on a Victor 3 plate reader (Perkin Elmer) ...
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bioRxiv - Cell Biology 2020Quote: ... HCT-OsTIR1 cells were co-transfected with these two constructs together with the PAF1-sgRNA expressing Cas9 plasmid using Fugene HD (Promega), and selected with 100 μg/ml Hygromycin B (Invivogen ...
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bioRxiv - Immunology 2021Quote: Cells were washed twice in D-PBS (Wisent) and lysates were collected using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The plasmids containing indicated CDS and tubP-GAL4 were co-transfected into the cells using FuGENE HD transfection reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... All cells in each well were lysed and luciferase was measured using ONE-Glo™ Luciferase Assay reagent (Promega, US). RLUs are per well of a 96-well plate.
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bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cytotoxicity on mock infected plates was determined using the Promega CellTiter-Glow Luminescent Cell Viability Assay (Promega, Madison, Wisconsin, USA). Half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Molecular Biology 2021Quote: ... and luciferase activity in 10 μl aliquots of the cell lysates was measured using the Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reporter gene expression was assessed in 20 µl of cell lysates using the Dual Luciferase Assay and a GloMax luminometer (Promega). To control for transfection efficiency ...
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bioRxiv - Biochemistry 2022Quote: HeLa and HEK293T cells were seeded on 18 mm glass coverslips at a density of 3×105 cells/mL and were transfected with FuGENE 6 (Promega) transfection reagent 24 h later ...
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bioRxiv - Genetics 2022Quote: ... instrument was configured with the Standard Elution Volume (SEV) hardware and using the Maxwell 16 Cell DNA purification kit (Promega). For total RNA isolation ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of lentiviral plasmid encoding doxycycline inducible transporters and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells with FuGENE 6 transfection reagent (Promega). Cell supernatant was replaced 24 h after transfection and harvested after another 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The mammalian expression vectors for EphA1 WT and EphA1 P460L were transfected into HEK cells using Fugene6 transfection kit (Promega). Expression of EphA1 WT or EphA1 P460L was confirmed by immunoblotting with anti-Flag or anti-Myc antibody.
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bioRxiv - Molecular Biology 2020Quote: ... the cells were collected and the luciferase activity was measured using the Dual-Glo Luciferase Assay system (Promega Madisson, USA) and normalized to Renilla Luciferase activity.
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bioRxiv - Molecular Biology 2021Quote: Cytotoxicity of BTZ in CCL-210 cells was assessed using a luciferase-coupled ATP quantitation assay (CellTiter-Glo from Promega). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: The ATP quantification assay was performed as per the protocol for the CellTiter-Glo 3D cell viability assay kit (Promega), using 1x 104 cells per sample ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated from cells at the exponential phase (OD600 ~1) with the acidic hot phenol method 41 and treated with DNase (Promega). 50 μg of total cellular RNA was incubated with 1 μM of the corresponding DNAzyme ((Supplementary Table S2 ...
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bioRxiv - Molecular Biology 2021Quote: ... the genomic DNA from all KO cell lines was extracted and the region flanking the Cas9 target site from each ΔPUS cell line PCR amplified and cloned into the EcoRI and HindIII sites of pGEM-3zf vector (Promega). The primers used to amplify the region flanking the Cas9 target sites were the following ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed 24 h after transfection and luciferase activities were measured with the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 luminometer (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... Transduction rates were assessed by measuring luciferase activity in cell lysates at 48 hours post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
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bioRxiv - Microbiology 2020Quote: ... Infection rates were assessed by measuring luciferase activity in cell lysates at 16 h post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
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bioRxiv - Molecular Biology 2021Quote: ... Then the plasmid constructs (150 ng) were transfected into HEK 293T cells at their 90% confluency using Dual-Luciferase Reporter Assay System (Promega). The Luciferase activity was measured 72 h (48h for DsiRNA and 24 h for plasmid constructs ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were then lysed with RIPA lysis buffer containing Protease Inhibition Cocktail and RNase Inhibitor followed by DNase treatment (Promega). The immunoglobin-coated protein magnetic beads (Thermofisher scientific ...
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bioRxiv - Bioengineering 2020Quote: The therapeutic effect of free taxane (pro)drugs and LNP formulations was determined by the [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega) or the resazurin-based PrestoBlue assay (ThermoFisher) ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... the gRNA/Cas9 plasmid as well as the plasmid carrying homology arms and mAID-mClover neomycin cassette were transfected into HCT116 CMV-OsTIR1 cells using FuGene HD (Promega). After 6 days selection with 700 µg/ml G418 the cells were FACS-sorted for mClover expression and seeded into 96 well plates to grow single clones ...
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bioRxiv - Genomics 2019Quote: ... Firefly luciferase and Renilla luciferase activities were measured in 10μl of HEK293T cell lysate using the Dual-Luciferase Reporter assay system (Promega, E1910) in a Veritas™ Microplate Luminometer ...
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bioRxiv - Genomics 2019Quote: 48 hours after transfection the HEK293T cells were washed 3 times with PBS and lysed in 100μl of Passive Lysis Buffer (Promega, E1910). Firefly luciferase and Renilla luciferase activities were measured in 10μl of HEK293T cell lysate using the Dual-Luciferase Reporter assay system (Promega ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were subsequently assayed for red Firefly activity using the ONE-GloTM Luciferase Assay System kit (Promega, Madison, WI) on a Spectramax M5 (Molecular Devices ...
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bioRxiv - Biochemistry 2019Quote: ... 500 ng of the lentiviral backbone plasmid containing the guide sequence and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 hours and was harvested after 48 hours ...
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bioRxiv - Plant Biology 2019Quote: ... ATP content was deter-mined for each sample through ATP-dependent luciferase reaction (BacTiter-Glo Microbial Cell Viability Assay, Promega) as indicated by the manufacturer.
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bioRxiv - Developmental Biology 2019Quote: ... The medium was supplemented with 1mM Luciferin (NanoLight Technology, Cat#306A) and 0.5µl of RealTime Glo Cell Viability Assay Substrate (Promega, Cat#G9711). For imaging in pluripotency maintenance conditions ...