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Citations for Promega :
5151 - 5200 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was generated in HEK293T cells by transfecting lentiviral transfer vector, and helper vectors (psPAX2, and pVSV-G) using Fugene HD (Promega). Lentiviral supernatant was collected 48 hours after transfection ...
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bioRxiv - Cancer Biology 2023Quote: Caspase3/7 activity was quantified in cell lysates using the Kit Caspase-Glo® 3/7 Assay Systems (G8091, Promega).
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 24hr at 37°C and cell death was measured through a lactate dehydrogenase (LDH) assay using the CytoTox 96 kit (Promega). 50 μL of media was collected from all experimental wells with two replicates each and placed in a fresh 96-well plate ...
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bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 24 h later the cells were incubated with a final concentration of 16 nM Halo-Ligand JF 549 (Promega, GA1110) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: – NFκB RE plasmid was transfected in A549-LV cells and the luciferase reporter assay was done according to Nano-Glo Luciferase Assay System protocol (Promega). TNFα was used as an activator and in some cases QNZ and TRAF2 siRNAs were also used.
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bioRxiv - Biochemistry 2023Quote: Cells were grown in opaque 96-well white plates and then processed according to the manufacturer’s instructions of the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega # G9241). The standard curve of ATP was determined in parallel for absolute quantitation.
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bioRxiv - Cancer Biology 2023Quote: The expression of inducible luciferase in cell lines was measured using the Dual-Glo Luciferase Assay system (cat. no. E2920, Promega). Cells were seeded at a density of 15,000 cells per well in a 96-well plate (3917 ...
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bioRxiv - Cell Biology 2023Quote: Promoter fragments containing the CCHCR1-TCF19 intergenic region were cloned from HeLa cell genomic DNA and inserted into the firefly luciferase (Fluc) reporter vector pGL4.17 (Promega, USA), in either forward or reverse orientation ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed 24 h after transfection and luciferase activities were measured with the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 luminometer (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... Necrosis was quantified by measuring release of lactate dehydrogenase in 30 µl samples of the medium and apoptosis by determining cell-associated Caspase3/7 activity using the respective kits from Promega Corp. ...
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bioRxiv - Microbiology 2024Quote: Lytic cell death was indirectly studied by measuring lactate dehydrogenase (LDH) release in cell culture medium using the CytoTox 96 nonradioactive cytotoxicity assay kit (G1780, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The stimulation was stopped by discarding the media and cell lysis was induced by addition of 1x passive lysis buffer (PLB, Promega) and horizontal shaking for 15 min at room temperature.
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted from pelleted bacterial cells grown in liquid YM medium using the Wizard Genomic DNA Purification System (Promega). Genes of interest were amplified from template DNA by PCR using GOTaq polymerase (Promega ...
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bioRxiv - Cell Biology 2024Quote: PDZD8-HaloTag knocked-in HeLa cells were incubated with 200 nM Janelia Fluor (JF) 549 dye (Promega, catalog no. GA1110) at 37°C o/n ...
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bioRxiv - Immunology 2024Quote: ... The supernatants from cells stimulated with inflammasome activators were collected and subjected to lactate dehydrogenase (LDH) assay to assess cell death using a CytoTox 96 non-radioactive cytotoxicity assay kit (Promega). Both the supernatants and cell pellets were collected for immunoblotting analyses.
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bioRxiv - Immunology 2024Quote: ... CellTiter-Glo luminescent cell viability assay kit (G7570) and CytoTox 96 non-radioactive cytotoxicity assay kit (G1780) were purchased from Promega.
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bioRxiv - Microbiology 2024Quote: ... The transfected cells were then lysed and analyzed for firefly and renilla luciferase activities using a GloMax 96 microplate luminometer (Promega). The function of ANP32 was examined using a polymerase assay by co-transfection of DKO cells with different ANP32 proteins or empty vector (20 ng ...
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bioRxiv - Cancer Biology 2024Quote: 1,000 HCECs or M158 cells were plated per well of 96-well plates and proliferation was measured over time using CellTiter Blue (Promega) and a plate reader ...
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bioRxiv - Microbiology 2024Quote: ... TC-tr minigenome rescue cells were lysed and processed using the Nano-Glo® HiBiT Lytic Detection System (Promega Corporation), according to the manufacturer recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... mammalian expression vectors containing the fusion gene under the control of a weak L30 promoter and containing a Neomycin resistance marker were transfected into U2OS cells at 70% confluence using FuGENE 6 (Cat. No. E2691, Promega). Transfected cells were selected with G418 (Cat ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell medium was removed and cells were washed in PBS 1X three times before lysis in 100 μL of Glo Lysis Buffer (Promega) for 5 min at RT ...
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bioRxiv - Cancer Biology 2024Quote: Human H1-0 promoter sequence (nucleotides −351 to +161 from TSS) was PCR-amplified from REH cell genomic DNA and inserted into Firefly luciferase vector pGL4.22 (Promega, #E6771) at KpnI and HindIII restriction sites ...
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bioRxiv - Cell Biology 2024Quote: Total accumulated cytotoxicity (an integrated readout for apoptosis and late apoptotic necrosis) was monitored in real-time using the Cell-tox assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of reporter vector plus 50 ng of the renilla luciferase plasmid was transfected to cells using FuGENE 6 (Promega); cells were then treated with DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were harvested 48 h after transfection and luciferase activity was measured using the Dual-Glo Luciferase Assay system (Promega) on a FlexStation® 3 plate reader ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested 48 hours after transfection and were assayed for the luciferase activity using the luciferase assay system (Promega) and a luminometer (BMG LABTECH ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were left to grow for 48 hrs of treatment (unless indicated otherwise) and then cytotoxicity was measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to manufacturer protocol with a Molecular Devices Spectramax M3 plate reader.
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bioRxiv - Cancer Biology 2024Quote: ... Positive cells were selected using antibiotic Zeocin and Luciferase reporter assay was performed using Dual- Glo luciferase assay kits (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... then transfected with pRK5-based constructs of iGluR subunits and Neto variants (1 µg total DNA/ ml cells) using ViaFect transfection reagent (Promega). The cells were incubated at 37°C for 3 hours ...
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bioRxiv - Neuroscience 2024Quote: ... the conditioned medium was removed from the transfected cells and replaced with aCSF (see LatA treatment) containing the Halo-tag dye (Promega, according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from bacterial cells grown overnight in MOKA-rich medium using the Wizard genomic DNA purification kit (Promega) or as described (62 ...
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bioRxiv - Microbiology 2024Quote: In vitro toxicity was assessed in HeLa cells (ATCC® CCL-2) using the ApoTox-GloTM Triplex Assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Quantification of cell-cell-cell fusion was performed by measuring luciferase expression as relative luminescence units (RLU) 1 min by mixing cell lysates with the Steady-Glo luciferase substrate (E2520, Promega) on a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Microbiology 2024Quote: ... Viral titers were measured by infecting MDCK cells and measuring Nanoluciferase activity 8 h later with the Nano-Glo Luciferase Assay System (Promega)(52).
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were determined by infecting TZM-bl-luciferase reporter cell lines and estimating 50% tissue culture infectious dose (TCID50) using the Bright-Glo luminescence reporter gene assay system (Promega)73 as directed by the manufacturer.
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MYB insufficiency disrupts proteostasis in hematopoietic stem cells leading to age-related neoplasiabioRxiv - Cancer Biology 2021Quote: RNA was extracted from sorted KSL cells using TRIzol and converted to cDNA using M-MLV reverse transcriptase (Promega, Madison WI). Quantitative PCR was performed using Taqman (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: Cell cytotoxicity was detected by measuring lactate dehydrogenase (LDH) levels using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). The manufacturer’s instructions were followed and the absorbance reflecting the LDH content in the cell supernatant was measured at 492 nm (600 nm reference ...
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bioRxiv - Microbiology 2020Quote: ... Transfection of HEK293T cells was performed at 80% confluence using 25ng of each plasmid construction mixed with 150nL of Fugene 6 (E2691, Promega, France) reagent per well ...
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bioRxiv - Developmental Biology 2021Quote: ... miPEPs sequences cloned into pF25A ICE T7 Flexi vector were expressed in vitro using TnT® T7 Insect Cell Extract Protein Expression System (Promega). For cells extracts and Drosophila extracts ...
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bioRxiv - Cell Biology 2020Quote: ... Cell growth and viability were measured on the 96 well white bottom plate as an end point measurement for each time point and/or treatment using the Cell-titer GLO assay (Promega, # G7570) based on manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Heterologous expression of proteins was achieved by transfecting cells with 1 □g of plasmid DNA/35 mm using FuGENE HD (Promega; E2311), as previously described (Webb et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 250 ng of each of the donor and Cas9-sgRNA vectors were transfected to RPE-1 cells cultured in 6 wells with 1.5 μl of FuGENE® HD (Promega, E2311) overnight ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatants were analyzed for LDH activity using the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega, Cat. no: G1780).
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bioRxiv - Genetics 2021Quote: ... cell supernatant from untransfected and cells transfected with IL2 constructs was diluted 1:12 with complete media and used to perform the IL2 Bioassay (Promega, JA2201). Luminescence (RLU ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with PBS and lysed directly on the plate on ice using the Cell Culture Lysis Reagent from the Luciferase Assay System (Promega#E1500). Lysates were transferred to microcentrifuge tubes ...
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bioRxiv - Neuroscience 2021Quote: ... neonatal and adult astrocytes plated on 96-well plates (50,000 cells per well) was measured with the Glucose Uptake-Glo™ Assay (Promega, J1341) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The expression of extracellular HiBiT-BNLN was normalized to the total intracellular pool of HiBiT-BNLN by lysing the cells and measuring luminescence with Nano-Glo HiBiT lytic detection system (Promega, N2422), following the protocol.
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bioRxiv - Molecular Biology 2021Quote: ... The release of lactate dehydrogenase (LDH) into the cell culture medium was quantified by bioluminescence using the LDH-Glo™ Cytotoxicity Assay kit (Promega). 10% Triton X-100 was added to untreated cells for 15 minutes to determine the maximum LDH release ...