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Citations for Promega :
4601 - 4650 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: HEK293T cells were transfected with an equal ratio of indicated GPCR plasmid and pGloSensor™-22F cAMP plasmid (Promega). 18 hours post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cell media was then replaced with a 1:1 mixture of fresh media and Nano-Glo Luciferase reagent (Promega) and luminescence was measured using a SpectroMax M5e (Molecular Devices) ...
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bioRxiv - Molecular Biology 2021Quote: ... Low passage MCF10A cells were transfected at 80% confluency in a 25 cm2 flask using 25 μl FugeneHD (Promega) with 6 μg donor plasmid (pAS4885) ...
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bioRxiv - Microbiology 2020Quote: Env-pseudotyped luciferase reporter viruses were generated by co-transfection of 810 ng of an env expression vector and 810 ng of pZM247Fv2Δenv backbone (22) using 293T cells and the Fugene 6 reagent and protocol (Promega). Five hours after transfection ...
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bioRxiv - Microbiology 2020Quote: ... 48 hours after transfection into 96 well plates in triplicates and cell number was measured by MTT assay (Promega) at 4 hours after addition of tetrazolium dye.
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bioRxiv - Microbiology 2021Quote: ... Cell viability was determined 24 h after the addition of compound-containing DMEM using the CellTiter-Glo kit (Promega) and a luminescence plate reader (Perkin Elmer) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells from each well were lysed using the Bright-Glo Luciferase Assay System (Cat#: E2610, Promega, Madison, WI, USA), and the cell lysates were transferred to 96-well Costar flat-bottom luminometer plates ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity in cell lysates were measured by using either Firefly or Renilla luciferase kits (Promega, Madison WI, USA), and light emission was measured in arbitrary light units on a Glomax 20/20 Luminometer (Promega ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were lysed and luminescence from each well was measured twice using a Glomax-Multi Detection System luminometer (Promega) and the Dual-Luciferase Reporter Assay (Promega ...
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bioRxiv - Genetics 2019Quote: The constructed expression plasmids were transfected into HEK293T and U2OS cells by using ViaFect™ transfection reagent (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids were recombined into 80,000 U-2 OS cells in a 24 well plate using 1.5 uL of FuGENE6 (Promega E2691) in 100 uL OPTIMEM (Fisher Scientific 31985070 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then lysed and firefly and Renilla luciferase activities assayed using the Dual Luciferase Reporter Assay System (Promega).
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bioRxiv - Developmental Biology 2020Quote: ... a colorimetric MTS assay was performed on HUVECs following the manufacturer’s protocol (CellTiter 96 AQ ueous One Solution Cell Proliferation Assay, Promega) in 96 wells ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were harvested 48 hours post-transfection and genomic DNA was extracted using the Wizard® SV Kit (Promega). Genomic targeting efficiency for each gRNA was determined through annealing and digestion with T7 Endonuclease I (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... per well of a 96-well plate 4×104 Drosophila SR2+ cells were seeded and transfected using Fugene (Promega) according to the manufacturer’s instructions with the following plasmids ...
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bioRxiv - Genetics 2020Quote: ... HEK293T/17 cells were transfected with firefly luciferase reporter constructs and a Renilla luciferase (Rluc) normalization control (pGL4.74; Promega) in a ratio of 50:1 ...
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bioRxiv - Immunology 2021Quote: ... The media from infected cells was then removed and 100 μl of 1:1 diluted PBS:Bio-Glo (Promega, G7940) luciferase substrate was added to each well ...
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bioRxiv - Neuroscience 2021Quote: ... equilibrated at 20°C for 30 minutes and 30 μl CellTiter-Glo Luminescent Cell Viability Assay reagent (Promega, #G7573) was distributed into each well ...
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bioRxiv - Immunology 2021Quote: ... Confirmed heavy and light chain plasmid DNA were co-transfected in 293T cells (ATCC) using Fugene transfection reagent (Promega) in 24 well plates for preparing antibody supernatant for initial screening for their expression and antigen specificity as detailed in the following section ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were harvested 24 h post transfection and luciferase assay was performed as per the manufacturer’s protocol (Promega, E2610). Readings for luminescence were taken in luminometer (Berthold ...
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bioRxiv - Cell Biology 2020Quote: Firefly and Renilla luciferases were assayed from whole cell lysates with the Dual-Luciferase® Reporter Assay System (Promega) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated as above except lysis was done in the presence of 100 units/ml RNasin (Promega, #N251B).
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bioRxiv - Microbiology 2021Quote: ... cells were lysed and NanoLuc luciferase activity was measured with Nano-Glo® Luciferase Assay System (Promega, Madison, WI) according to manufacturer’s instructions using a Synergy Neo 2 plate reader (BioTek ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and the lysate was used in dual luciferase assays using the Dual-Glo luciferase system (Promega), and measured on the GloMax Discover plate reader (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... HEK cells were transfected with 5’UTR-reporters for 24h using ViaFect reagent according to the manufacturer’s protocol (Promega). Next ...
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bioRxiv - Immunology 2021Quote: ... the luciferase activities of infected HEK293T/ACE2 cells were detected by the Bright-Luciferase Reporter Assay System (Promega, E2650). The IC50 of the evaluated mAbs was tested by the Varioskan LUX Microplate Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: Nitric oxide was quantified from cell supernatants using the Griess Reagent System according to the manufacturer’s instructions (Promega G2930). For these experiments ...
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bioRxiv - Immunology 2020Quote: ... The sleeping beauty transposon system was stably transfected into HEK293 EBNA cells using FuGENE® HD (Promega GmbH, USA) in DMEM/F12 (Merck ...
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bioRxiv - Immunology 2020Quote: ... The identity of the Huh7 cells used in this study was verified by using the GenePrint STR kit (Promega) (DNA Analysis Facility ...
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bioRxiv - Biochemistry 2020Quote: The microbial viability was assessed in equalized bacterial cultures using the BacTiter-GloTM Microbial Cell Viability Assay kit (Promega) according to the manufacturer’s recommendations and luminescence was measured using a VarioSkan plate reader (Thermo Scientific).
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bioRxiv - Biophysics 2020Quote: ... All NanoLuc assays in this study were carried out with Nano-Glo® Live Cell Assay from Promega (N2011) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The identity of the Huh7 cells used in this study was verified by using the GenePrint STR kit (Promega) (DNA Analysis Facility ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell culture medium was replaced by pH indicator-free culture medium containing 0.32 mg/ml MTS (Promega, Mannheim, Germany) and 0.0073 mg/ml phenazine methosulfate (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... twice and 20 µL of a 1:19 mixture of substrate:buffer from the Nano-Glo Live Cell Assay (Promega) were added to 80 µL of OptiMEM per well ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was assayed with CellTiter-Glo according to the manufacturer’s protocol using a Spectramax M5 plate reader (Promega).
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bioRxiv - Cell Biology 2022Quote: ... knock-down and control cells were treated with IKE for 18h and viability was measured by adding CellTiter-Glo 2.0 Reagent (Promega) into each well and measuring luminescence in an EnVision 2104 Multilabel plate reader (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... containing NanoLuc substrate was added to the living cells (40 µl/well, containing 1.0 µl of NanoLuc substrate stock from Promega). Thereafter ...
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bioRxiv - Cancer Biology 2022Quote: ... The anti-proliferative effects of these compounds were assessed using the Cell Titer Glo assay kit (Promega Cat. # G7570) following manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and the viability of the cancer cells was assessed by measuring luminescence with a GloMax Discover plate reader (Promega) or an IVIS Lumina III XR (Perkin-Elmer ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed with 50 µl of PBS and lysed with 40 µl of Renilla lysis buffer (Promega, E2820) for 30 minutes with agitation ...
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bioRxiv - Biophysics 2022Quote: ... and 0.167μg per 35mm well) into 293T cells grown to approximately 80% confluency using Mirus TransIT-293 Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... into a 10 cm plate of HEK293T cells at 60-70% using Fugene 6 transfection reagent (Promega, Cat #E2691). Media containing lentiviral particles were collected at 24 ...
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bioRxiv - Cancer Biology 2022Quote: ... Spheroids were imaged by a phase contrast microscope and cell viability was measured by CellTiter-Glo according to manufacturer’s guidelines (Promega).
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bioRxiv - Cell Biology 2022Quote: ... The GAST-driven firefly luciferase constructs were transfected in EndoC-βH1 cells along with phRL-TK internal control (Promega) using the Lipofectamine protocol (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: A fragment of human BGT1 promoter region (nucleotides −1732 to +22 of NM_003044.5) was cloned from a gDNA pool derived from HeLa cells and subcloned into pGL4.10[Luc2] (Promega, Cat#E665A). Two sites of NFAT5 binding motif (TGGAAANNYNY ...
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bioRxiv - Immunology 2022Quote: ... A549 cells were transfected with PX459 or PX458 plasmids containing guide RNAs using FuGENE HD transfection reagent (Promega E2311) following manufacturers guidelines ...
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bioRxiv - Genomics 2022Quote: ... expression plasmids were co-transfected with GAG/POL and VSVG plasmids into HEK 293T cells using FuGENE HD (Promega). Media was exchanged after 6-10 h ...
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bioRxiv - Immunology 2022Quote: ... the lentivirus was produced by transfecting 70% confluent HEK 293T cells with the desired plasmid along with psPAX2 and pMD2.G following the manufacturer’s instructions (FuGENE HD, Promega). The virus-containing medium was collected 48 h after transfection ...
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bioRxiv - Immunology 2022Quote: ... the lentivirus was produced by transfecting 70% confluent HEK 293T cells with the desired plasmid along with psPAX2 and pMD2.G following the manufacturer’s instructions (FuGENE HD, Promega). The virus-containing medium was collected 48 h after transfection ...