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Citations for Promega :
4551 - 4600 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cells were diluted to a final concentration of 500,000 cells/mL in Expi293 expression medium plus 2% (v/v) Glosensor reagent (Promega) and incubated for 75 minutes at room temperature with gentle rotation ...
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bioRxiv - Cancer Biology 2022Quote: The 4T1-mScarlet and 67NR-GFP cell lines were generated by transfection using a 1:4 ratio of plasmid DNA:FugeneHD reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed and the level of infection was assessed using the Luciferase Assay System (Cat: E1501, Promega Co.). The manufacturer’s protocol was modified by putting the cells through 3 freeze/thaw cycles after the addition of 100 μl of the lysis reagent ...
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bioRxiv - Synthetic Biology 2022Quote: ... followed by injection in PBS of a combination of 40x dilutions of Nano-Glo live cell imaging (Promega, N2011) and Nano-Glo (Promega ...
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bioRxiv - Cancer Biology 2022Quote: Viability experiments to test the resistance phenotypes (here defined as fitness) were performed using the CellTiter-Glo 2.0 Luminescent Cell Viability Assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 50 μl of tissue or cell lysates were mixed with 50µl of Bright-Glo™ Luciferase Assay System (Promega) in a 96-well luminometer plate ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
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bioRxiv - Genomics 2022Quote: Cell viability upon siRNA transfection was measured through the CellTiter 96® Non-Radioactive Cell Proliferation Assay (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT)) (Promega). 1× 104 microglia cells were plated per well in a 96 well plate and transfected with either ON-TARGETplus Non-targeting Control siRNA (Horizon Discovery) ...
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bioRxiv - Microbiology 2022Quote: ... and cells were rinsed in 1X PBS and lysed by the addition of 40 µl passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Cell Biology 2022Quote: ... cells were plated 24 hours prior to transfection and the transfection was carried out using FuGENE® HD (Promega) or Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2022Quote: ... NanoBRET™ Nano-Glo® Detection System and CellTiter-Glo® Luminescent Cell Viability Assay was purchased from Promega.
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bioRxiv - Cancer Biology 2022Quote: ... cell extracts were prepared and the luciferase activity was measured by the Dual-Luciferase Reporter Assay System (Promega, USA). The primers used for the cloning HMGN5 promoter and its mutant are listed below.
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bioRxiv - Microbiology 2022Quote: ... 100 μl of spent medium was removed and 100 μl of the Cell Titer Glo assay reagent (Promega Inc.) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... Firefly and Renilla luciferase reporter gene expression in the cells were measured with a Dual Luciferase Assay System (Promega Corporation ...
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bioRxiv - Microbiology 2022Quote: ... 40 μL of the cells’ supernatant containing secreted GLuc were mixed with Renilla luciferase Assay Reagent (100 μl) (Promega). GLuc activity was measured using SpectraMax i3 Multi-mode Detection Platform (Molecular Devices).
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bioRxiv - Biophysics 2022Quote: Plates were left to equilibrate at room temperature for 20 minutes before addition of NanoGlo live cell substrate (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded on 35mm Petri dishes at a concentration of 50,000 cells per dish and transfected after 24h using FuGene HD (Promega).
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bioRxiv - Microbiology 2021Quote: ... and Tat-dependent increase of luciferase enzyme activity in cell lysates was determined with the Luciferase Assay system (Promega). Luminometric activity was analyzed with a Mithras luminometer.
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with YFP-FKBP-Luciferase and PACT-Ce3-FRB plasmids using Fugene HD transfection reagent (Promega) 20-24 hr before synchronization ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested and luciferase activity was measured using the Dual-Luciferase® Reporter Assay System (Promega, Cat#: E1960) in a Turner Biosystems Modulus Microplate Reader ...
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bioRxiv - Molecular Biology 2020Quote: ... After 48hr cells were washed with PBS and lysed in a sonication bath in 1x Passive Lysis Buffer (Promega) supplemented with Protease Inhibitor cocktail (Roche) ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with WT or mutant C10 constructs using the FuGENE 6 transfection reagent (Promega, cat# PRE2691). The cells were incubated at 37 C at least overnight before downstream analysis.
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bioRxiv - Cancer Biology 2021Quote: ... cells were passively lysed and processed according to the protocol of the Dual-Luciferase® Reporter Assay System (Promega), besides using only half the recommended volume of detection buffers ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed 48-hrs later for FLuc quantitation using a Luciferase 1000 Assay System kit (Promega Cat#E4550) according to manufacturer’s instructions and read on a Veritas luminometer ...
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bioRxiv - Cancer Biology 2021Quote: ... medium was aspirated from each well and 25 μl of Nano-Glo Live Cell Reagent was added to each well per the manufacturer’s protocol (Promega). Plates were placed on an orbital shaker for 1 min at 300 rpm ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed using passive lysis buffer and luciferase activities were measured using Luciferase® reporter assay system (Promega) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... Phospho-Tyrosine specific antibodies (anti P-Tyr674/675 from Cell Signalling 1:3000) and anti-p75 intracellular antibody (Promega) were used ...
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bioRxiv - Microbiology 2021Quote: ... virus was harvested and infectivity assayed by serial titration on HeLa TZM-bl reporter cells using Bright-Glo (Promega).
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysis and detection of firefly luciferase were performed according to the protocol recommended by the manufacturer (Promega Inc). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were lysed and the luciferase activity was measured by the dual-reporter assay (Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with the indicated drugs in combination with pan-caspase inhibitor Z-VAD-FMK (10 mM, Promega). After 24 hours ...
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bioRxiv - Plant Biology 2022Quote: ... Luciferase activity of cell extracts was measured with Sirius L Tube Luminometer (Berthold) and the luciferase assay system (Promega).
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bioRxiv - Physiology 2022Quote: ... Cells were then air-dried for 30 min and lyse with 200 μg/ml proteinase K (Promega, WI, USA) in lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: The cell proliferation of U87MG and H4 was observed using CellTiter 96® AQueous One Solution Proliferation Assay (Promega). Cells were cultured in a 96-well plate for 72 hours under activation and AZD8055 treatment conditions ...
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bioRxiv - Cancer Biology 2019Quote: Cell growth was assessed using Proliferation Assay™ Kit in accordance with the manufacturer’s instructions (Promega, Madison, WI, USA) [15 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were allowed to grow for two doubling times and proliferation was measured by CellTiter-Glo® (Promega G7571) or CyQuant (Invitrogen C3511 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 50 μL of cell lysate from each sample to 50 μL of the GSH-Glo™ Reagent 2X (Promega) with DTT (~2.5 mM final ...
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bioRxiv - Molecular Biology 2020Quote: ... Hygromycin (150ug/ml) was used to select for cells with integration and Nano-Glo Dual-luciferase assay kit (Promega) was used to confirm expression of both Firefly and Nano-luciferase in clonally isolated cells.
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bioRxiv - Pathology 2020Quote: Cells isolated from the whole constructs were used for RNA isolation using ReliaPrepTM RNACell Miniprep System (Promega, Madison, WI) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... NLRP7 or GFP expressing vectors were transfected to 293FT cells at a density of 2,5×106 in 10cm dishes to produce lentiviruses using Fugene (Promega). Virus containing medium were collected 2 days ...
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bioRxiv - Biophysics 2019Quote: hTERT RPE-1 cells stably expressing Smo-Halo were transfected with OMP25-SNAP using FuGENE HD Transfection Reagent (Promega) one day before imaging ...
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bioRxiv - Biophysics 2019Quote: ... hTERT RPE-1 cells stably expressing Smo-Halo were transfected with Sec61β-SNAP using FuGENE HD Transfection Reagent (Promega) one day before imaging ...
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bioRxiv - Cancer Biology 2019Quote: ... CellTiter 96® AQueous One Solution Cell Proliferation Assay and Dual-Glo® Luciferase Assay were purchased from Promega Corporation.
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bioRxiv - Molecular Biology 2020Quote: ... or day 4 in TZM-bl cells by measuring luciferase activity using the luciferase assay system (Promega, Madison, WI) and the microplate luminometer Monolight 3096 (BD Biosciences ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was estimated five days after transfection using a luminescent assay detecting cellular ATP levels (CellTitre-Glo, Promega). Luminescence values were processed using the cellHTS2 R package ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cells were harvested and tested for luciferase activity using a Dual-Luciferase® Reporter Assay System (Promega, CAT# E1980). Luciferase activity was determined with the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and were frequently tested for absence of mycoplasma and cell lines were authenticated using STR profiling kit from Promega.
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bioRxiv - Pathology 2021Quote: ... Cytopathic effects of the virus were measured after 3 days using the CellTiter-Glo luminescent cell viability assay (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed and the luciferase activities were measured using the GloMax® 96 Microplate Luminometer (Promega, GMJ96).
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bioRxiv - Molecular Biology 2020Quote: ... and 0.5 ml (approximately 2 × 109 cells) were used for gDNA extraction with the Wizard Genomic DNA Purification kit (Promega), which typically yielded 50 μl of 0.5-1.5 μg/μl gDNA.