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Citations for Promega :
4651 - 4700 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 20 μL of cells were mixed with 20 μL of the CellTiter-Glo 2.0 Cell Viability Reagent or Caspase-Glo 3/7 Assay substrate (Promega). Luminescence was measured after 2 min incubation for CellTiter-Glo 2.0 or 30 min incubation for Caspase-Glo 3/7 Assay using the Synergy H1 microplate reader as above ...
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bioRxiv - Immunology 2022Quote: ... Dual expression of mCherry-eGFP in pmChGIP was confirmed following transfection into HEK293T cells with FuGENE HD (Promega, E2311) (Figure S1c) ...
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bioRxiv - Molecular Biology 2022Quote: The activity of the NF-κB luciferase reporter cell line was measured according to the manufacturer’s protocol (ONE-GloTM Promega) into 96-well plates with white walls and bottom to reduce the cross-contamination of the signal between wells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Assessment of SL drug cytotoxicity in 3D samples was performed using a CellTiter 3D Cell Viability Assay (Promega, #G9681) following manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cells were preincubated with 50 µl of a mix of Nluc substrate endurazine (1:50 dilution; #N2571, Promega) and LgBiT (1:50 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were subjected to the luciferase assay using the Duel-Glo Luciferase Assay System following the manufacturer’s protocol (Promega). For autophagy activity assays ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was extracted from 293T cells using an Eastep Super Total RNA Extraction Kit (LS1040, Promega, WI, USA). Reverse transcription was conducted using GoScriptTM Reverse Transcription Mix and Random Primers (A2800 ...
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bioRxiv - Biophysics 2022Quote: Cell viability assays were carried out following manufacturer’s instructions from the CellTiter-Glo 2.0 assay from Promega (Madison, USA). In brief ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA was isolated from 50 μl of cell suspension with the Wizard SV Genomic DNA purification system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then transfected with each reporter construct (450ng) and 50ng pGL4.74 Renilla luciferase expression vector (Promega; Madison, WI) using ViaFect Transfection Reagent (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 320 ng 12x MS2-tagged repeat expression construct and 80 ng CMV-BFP plasmid DNA (for labeling transfected cells) mixed with 1.6 μl FuGENE HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of cells was then processed using the Promega Wizard® genomic DNA preparation kit (Promega, WI, USA) to obtain genomic DNA ...
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bioRxiv - Molecular Biology 2022Quote: HEK-293T cells were plated in a 24-well plate at 400,000 cells per well before being transiently transfected with pGL3-control (Promega), a construct constitutively expressing a firefly luciferase ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected at 24 h after transfection and luciferase activity was measured with the Dual-Luciferase Assay (Promega) with a Luminoskan Ascent luminometer (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Dual Luciferase reporter assay kit and CellTiter96 Aqueous one solution cell proliferation assay kits were from Promega (Madison, USA). Non-targeting siRNA (Catalog no ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in plate and luciferase activity was measured using Luciferase 1000 Assay System (Cat # E4550, Promega, WI).
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bioRxiv - Cell Biology 2022Quote: ... Transfections were performed after letting the cells adhere to the glass for at least 12 hours using Fugene6 (Promega) at a 3:1 Fugene (ul ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were harvested and luciferase activity was measured using the Dual-Luciferase® Reporter Assay System (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then transfected with plasmid DNA (4µg per plasmid per flask) using FuGENE HD (1:3 ratio; Promega) and cultured at 28°C.
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bioRxiv - Cancer Biology 2024Quote: ... the cells were was lysed and Cell lysates were subjected to dual reporter luciferase assays according to the manufacturer’s instructions (Promega).
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bioRxiv - Microbiology 2024Quote: ... Luciferase activity in the cell lysates was determined using the Dual-Glo luciferase assay system (Promega, Madison, WI, USA). The degree of cell entry was directly proportional to the Renilla luciferase units and normalized to firefly luciferase activity.
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bioRxiv - Molecular Biology 2024Quote: ... 400 μl cold cell lysis buffer (20 mM HEPES, pH 7.4, 100 mM KCl, 5 mM, MgCl2, 500U/ml RNasin-Plus (Promega), 1x protease inhibitor cocktail (200X ...
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bioRxiv - Cell Biology 2024Quote: ... Aliquots of the cell lysates were used to read luciferase emission using Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The Cas9-sgRNA vector and ssODN were transfected into cells for gene editing using FuGENE HD (Promega, Cat# E2311) transfection reagent ...
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bioRxiv - Genetics 2024Quote: ... cell lysates were collected and Firefly and Renilla luciferase signals measured using the Dual-Luciferase Reporter Assay System (Promega) as per manufacturer’s protocol on Tecan infinite 200 PRO ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Authenticity of cell lines was evaluated by detection of ten genetic loci using the GenePrint® 10 System (Promega) and cross referencing to ATCC or internal genetic profiles.
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: A validated CDHR2 KO CL4 cell clonal population was transfected with pHALO-N3-CDHR2 using FuGENE 6 (Promega #E2691) at a FuGENE:DNA (μL:μg ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were grown for 72 hours before equilibrating to room temperature and addition of 12.5 µL CellTiter-Glo Luminescent Cell Viability Assay reagent (Promega). At room temperature and protected from light ...
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bioRxiv - Cell Biology 2023Quote: ... were PCR-amplified from genomic DNA extracted from EndoC-βH3 cells with primer sets (Table S5) designed by Primer3-based software and cloned into pGL4.23[luc/minP] vector (Promega) between KpnI and XhoI restriction enzyme sites ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were collected and luciferase activity was determined using the DualLuciferase reporter assay system (Promega, Madison, WI, United States) with the dual luciferase assay reporter-ready luminometer (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by centrifugation and lysed with the Passive Lysis Buffer of the Dual-Luciferase detection kit (Promega). 10 µl cell extract was mixed with an equal volume of firefly luciferase assay substrate from the kit ...
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bioRxiv - Biochemistry 2024Quote: ... Viability of MV-4-11 cells was assessed using the CellTiterGlo 2.0 kit from Promega (as per manufacturer instructions); viability of HPAFII cells was assessed using PrestoBlue reagent from Thermo Fisher (as per manufacturer instructions) ...
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bioRxiv - Biochemistry 2024Quote: pcDNA5/FRT/TO-SFB-HELB (WT, D506G, and 3xA) plasmids (1 µg) were transfected into 1.0 × 105 cells using FuGENE HD (Promega) according to the manufacturer’s protocol followed by incubation at 37°C with 5% CO2 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... organoid viability was measured by using a luminescence-based CellTiter-Glo 3D® cell viability assay kit (G9681, Promega) and recording luminescence intensity with a microtiter well plate reader (BioTek® Cytation 5).
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bioRxiv - Cell Biology 2024Quote: ... The CellTiter-Blue® Cell Viability Assay was then performed according to the manufacturers’ protocol (Promega, Madison, Wi, USA). Plates were read using SpectraMax™ i3/i3x multi-mode plate reader.
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bioRxiv - Cell Biology 2024Quote: ... and then cells were homogenized according to the manufactureŕs instructions (Dual-Luciferase resporter assay system, Promega, Madison, WI, USA)
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bioRxiv - Cell Biology 2024Quote: 1μg of each plasmid or pcDNA3.1 empty vector was reverse transfected into 1x106 HEK293T cells using FUGENE HD (Promega) as per manufactures instructions ...
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bioRxiv - Biochemistry 2023Quote: BromoTag cell lines were generated in HEK293 cells via simultaneous transfection of two vectors at a 4:1 reagent:DNA ratio with FuGENE 6 (Promega). The first vector was a pMK-RQ vector containing 500 bp homology arms on either side of either an eGFP-IRES-BromoTag or eGFP-IRES-HiBiT-BromoTag sequence for integration into MCM4 and BRD4 ...
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bioRxiv - Cancer Biology 2023Quote: Total GSH levels in cells were detected by using luminescence-based GSHGlo Assay reagent kit (Promega, catalogue no. B6912) as instructed by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (E1910, Promega corporation, Madison, WI), and subsequently used to infect suspension cultures of Sf9 cells (2 × 106 cells/ml ...
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bioRxiv - Microbiology 2023Quote: ... after which the cells were lysed and luciferase reporter gene expression was measured using Bright-Glo luciferase reagent (Promega) and a Victor 3 or GloMax Navigator luminometer (Perkin Elmer/Promega) ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: 2′3′-cGAMP (0.2 μM) and dsDNA (2 μg/mL) was transfected into cells using FuGENE HD Transfection Reagent (Promega), whereas the transfection reagent without dsDNA or 2′3′-cGAMP was added as the control ...
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bioRxiv - Microbiology 2023Quote: ... viral titers were determined by infecting MDCK cells and measuring reporter activity using the Nano-Glo luciferase assay (Promega)86,91 ...
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bioRxiv - Microbiology 2023Quote: ... 48 hours after transduction cells were assessed for luciferase activity using the Promega Steady-Glo system (Promega Madison, WI).
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bioRxiv - Molecular Biology 2023Quote: ... the cells were harvested and the medium was exchanged to CO2 independent medium (Life Sciences) containing Endurazine substrate (PROMEGA) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: Cells were washed twice with Dulbecco’s PBS 1x then lysed with the lysis buffer cell culture Lysis Reagent 1X from Promega and centrifugated 10 min at 15000g ...
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bioRxiv - Microbiology 2022Quote: ... cells were harvested and both firefly and renilla luciferase activity measured with the Dual-Luciferase Reporter Assay System (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured for 24 h with a complex of DNA and FuGENE® HD Transfection Reagent (#E2311; Promega) at a ratio of 1:3 prepared in Opti-MEM reduced serum medium (#31985070 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 30 min at 4 °C slowing rotating with Passive Lysis Buffer (Promega Cat#E1941). After incubation with the lysis buffers ...