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Citations for Promega :
4451 - 4500 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... followed by addition of 25 uL/well of Antibody Dependent Cellular Cytotoxicity (ADCC) Bioreporter Cells (Promega cat# G7010). The ADCC Bioreporter cells are engineered Jurkat cells stably expressing FcγRIIIa V158 (high affinity ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmid DNA by the calcium phosphate or lipofection method using FuGene 6 (Promega, WI) (Yoshida et al. ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells were transfected with either Tau-encoding or control constructs using Fugene 6 (Promega, cat. no. E2691) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: KO pool cells were collected and gDNA was extracted using Wizard® Genomic DNA Purification Kit (#A1120, Promega) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell culture identity verification was done by short tandem repeat profiling (GenePrint® 10 System, Promega #B9510).
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bioRxiv - Cancer Biology 2023Quote: ... and wells were assessed for total ATP content using a commercial proliferation assay (Cell TiterGlo; Promega Madison, WI). Three biologic replicates were performed ...
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bioRxiv - Genetics 2023Quote: ... Cells were lyzed and assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega, Fitchburg, WI). Firefly luciferase activity was normalized to Renilla luciferase activity as relative light units (RLU) ...
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bioRxiv - Microbiology 2023Quote: Cell supernatants were collected and stored in LDH storage buffer + Triton 10% as per the manufacturer’s instructions (Promega). Supernatants were inactivated for 2 hours at room temperature prior to usage outside the BSL-3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Proliferation was measured every 24h by treating cells with 30 μL of the CellTiter Glo kit reagent (Promega) and measuring luminescence with a CLARIOstar Plus microplate reader (BMG Labtech).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were transfected with the following plasmids and amounts per well: 25 ng SRE-Luc reporter (E134A, Promega), 75 ng Gαi or Gαi QL in pcDNA3.1+ ...
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bioRxiv - Immunology 2023Quote: ... B lymphocyte proliferation was measured using the cell proliferation assay kit (CellTiter 96 Aqueous) from Promega (Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated with 0.7 nM Janelia fluor 646 dye conjugated to Halo-tag ligand (JF646; Promega) in the dark at 30°C for 15 min and washed with plain PYE to remove excess dye ...
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bioRxiv - Neuroscience 2023Quote: Intracellular ATP in assembloids was measured using luminescence based CellTiter-Glo® 3D Cell Viability Assay (Promega, G9681). Three organoids per cell line and per condition were transferred each in one well of the imaging plate (PerkinElmer ...
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bioRxiv - Systems Biology 2023Quote: HEK293 cells stably expressing μOR or μOR-APEX2 were transiently transfected with the cAMP biosensor pGLO-20F (Promega). Prior to agonist stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... 72-hour viability assays were performed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, G3580) following manufacturer recommendations and 490nM absorbance measured using a CLARIOstar plate reader (BMG Labtech) ...
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bioRxiv - Cancer Biology 2023Quote: ... and their numbers were measured every 2–4 days using the CellTitre-Glo 3D cell viability assay (Promega), as described by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... molar equivalents of the appropriate TF constructs were transfected into cells alongside a 1:1 molar ratio of Luciferase (pGL4.23) to Renilla (pGL4.75, Promega) vectors ...
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bioRxiv - Molecular Biology 2023Quote: HeLa or HEK cells were cultured for at least one day and transfected using FUGENE HD reagent (Promega) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 µl of CellTiter-Glo® reagent from CellTiter-Glo® 3D cell viability assay kit (Promega-UK) was added to each well and mixed for 5 minutes on a plate shaker and incubated at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... We then mixed the cell solution with 80 μL of NanoGlo luciferase assay reagent (Promega, Cat. No. N1110), transferred it to a 96-well plate well (PerkinElmer,Cat ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with LDLR-GFP (WT, C75W, and C75W/C34W) for 48 h with Fugene 6 (Promega). Cells were starved in 5% LPDS media (Millipore Sigma ...
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bioRxiv - Biophysics 2023Quote: ... the cells were co-transfected with FGFR1-ECTM-eYFP and FGFR1-ECTM-mCherry using Fugene HD (Promega # E2311) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Luciferase activity was recorded by mixing equal volumes of cell lysate and Steady Glo Reagent (Promega, Madison, WI) and reading in a Synergy H1 plate reader (Biotek ...
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bioRxiv - Biophysics 2023Quote: Dual luciferase assays were conducted after lysing the transfected cells using the Dual-luciferase Reporter Assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... ATP content was measured using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega Corporation, Madison, WI, USA) or the Caspase Glo® 3/7 Assay (Promega Corporation ...
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bioRxiv - Genomics 2023Quote: ... cells were transfected with 1µg L1 plasmid per well using 3µL FuGENE HD transfection reagent (Promega, Cat# E2311) and 97µL Opti-MEM (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Apoptotic cell count was measured as described above using the Caspase-Glo® 3/7 3D Assay (Promega). Bliss synergy scores were calculated using the SynergyFinder web application (version 3.0)61.
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... membranes were reblotted to detect β-actin expression using primary (Cell Signal #3700) and secondary-HRP (Promega #W402B) antibodies and developed as described.
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bioRxiv - Immunology 2024Quote: ... and cells were treated with 3µg/ml LPS O111:B4 and 0.25% (vol/vol) Fugene HD Plus (Promega) to cause transfection ...
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bioRxiv - Neuroscience 2024Quote: ... Cell viability was assessed 72 hours post-treatment using the CellTiter-Glo Assay (cat. # G7572, Promega, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were lysed and the firefly luciferase and Renilla activities were measured on a GloMax Luminometer (Promega) using a Dual-Glo Luciferase Assay System (Promega).
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bioRxiv - Systems Biology 2024Quote: PDTOs were dissociated into single cells when prepared for imaging and CellTiter-Glo (CTG; Promega, Madison, WI, G9681) cell viability assays ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then lysed 7 h later in 20 µl of Renilla luciferase assay lysis buffer (Promega), and luciferase activity was quantified using a Tristar LB 941 luminometer (Berthold Technologies ...
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bioRxiv - Cell Biology 2024Quote: Hap1 cells were grown on coverslips and then incubated with 400 nM Janelia Fluor HaloTag ligand 646 (Promega) for 30 minutes then washed three times with media and incubated in fresh media for 30 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ATP levels in motor neurons were assessed using the bioluminescent reagent Cell Titer-Glo 2.0 (Promega, Madison, WI). CT and NFIAcKO E13.5 mouse spinal cords (whole and ventral ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using the Maxwell® RSC simplyRNA Cells and RNase-free DNase Kit (Promega; AS1390) using an automated RNA/DNA isolation instrument (Maxwell ...
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bioRxiv - Microbiology 2024Quote: ... the cells were collected and infectivity was determined by luciferase assay according to the manufacturer’s instructions (E1500, Promega).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed 24 hours after transfection and the lysates were subjected to dual-luciferase reporter (DLR) assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cells or murine liver tissue using the Relia Prep RNA Tissue Miniprep System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Hela cells were co-transfected with CRISPR-Cas9 plasmids and a plasmid conferring puromycin resistance using FuGENE (Promega, E2693). 24 h after transfection ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells were cultured on 12-well plates and transfected with the indicated plasmids using Viafect (Promega, Madison, WI). After expression for 24h ...
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bioRxiv - Cell Biology 2020Quote: ... and harvested at varying timepoints by resuspending the cells in 50-100 μL of 1X Passive Lysis Buffer (Promega). Inductions were staggered so that all timepoints could be harvested simultaneously ...
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bioRxiv - Microbiology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in supernatants using CytoTox® Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... firefly and Renilla reniformis luciferase activities from lysed cells were determined using the dual-luciferase reporter assay system in accordance with the manufacturer’s protocol (Promega). Data are expressed as the relative firefly luciferase activities normalized to the activity of the R ...
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bioRxiv - Genetics 2022Quote: ... the SEV hardware was replaced with the Low Elution Volume (LEV) hardware and the Simply-RNA Cell kit (Promega) was used ...
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bioRxiv - Genetics 2022Quote: ... Firefly and Renilla luciferase activity of cell lysates were sequentially assayed using the Dual-Luciferase Reporter Assay System (Promega) on a GloMax Explorer luminometer (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were then transfected with mCherry fusions of actin or Lifeact variants using the FuGENE transfection reagent (Promega). The cells were grown for a further 24 h and transferred to an LSM 800 microscope (Zeiss ...
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bioRxiv - Molecular Biology 2020Quote: Gently re-suspend cells in 100 μl of 3% Glyoxal fixation solution with 1:25 RNasin Plus (Promega N261B) and incubate for 15 minutes on ice.
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bioRxiv - Bioengineering 2022Quote: ... The cell viability was investigated through the LDH dependent CytoTox 96 Non-radioactive Cytotoxicity assay (#G1780, Promega, Dübendorf, Switzerland.) and the ATP dependent CellTiter-Glo® 2.0 Cell Viability Assay (#G9241 ...
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bioRxiv - Immunology 2022Quote: Replication competent HIV were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...