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Citations for Promega :
4401 - 4450 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Luminescence was measured in cell lysates 24 h after transfection using the Dual Luciferase Reporter Assay Kit (Promega) on a Victor X luminometer (Perkin Elmer ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed and the luciferase activity was quantified using the Nano-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... were also sequenced using Illumina short-read technology at the IP using the equipment and services of the iGenSeq platform at the Institut du cerveau et de la moëlle épinière (Paris, France) from genomic DNA extracted with the Maxwell 16-cell DNA purification kit (Promega) in accordance with the manufacturer’s recommendations.
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bioRxiv - Microbiology 2022Quote: ... and then measured luciferase activity in cell lysates by using the Steady-Glo luciferase assay system (Promega Corp.) and a Tecan plate reader ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were lysed with 0.05% aqueous SDS and luminescence was measured using the GloMax 20/20 Luminometer (Promega) [15].
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bioRxiv - Cell Biology 2024Quote: ... Stable 3T3-L1 cell lines were generated in a similar manner using the transfection reagent Viafect (E4981; Promega), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cells plated on glass-bottomed dishes were treated with Janelia Fluor 646 HaloTag Ligand (Promega, Cat. No. GA1120) at a final labelling concentration of 200nM (in cell culture media) ...
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bioRxiv - Cancer Biology 2024Quote: ... Treatment effects were quantified 72 hours after cell seeding using calorimetric ATP measurement with CellTiterGlo 2.0 (Promega, G7572) according to the manufacturer’s protocol and as described previously (Peterziel et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... Luminescence levels yielded by cell lysates were measured using the Luciferase Assay System kit (Promega, Madison, WI, USA) in a Mithras LB 940 plate reader (Berthold Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed with HBSS and labeled with 100 nM HaloTag NanoBRET 618 ligand (Promega Corp., Fitchburg, WI) in phenol red-free Opti-MEM I reduced serum medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Cell viability post-drug treatment was measured via both MTT or WST assays according to manufactorer’s protocol (Promega) and automatic cell counter measurements (Luna II automatic cell counter) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected at 48 h post-infection and virus infectivity were measured by luciferase assay (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... then washed with 1 ml of PBS and lysed using 60 μl of Cell Culture Lysis Reagent (Promega). 20 μl of cell lysate was added to 20 μl of Luciferase Substrate (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... we supplemented culture medium with 10 μL of CellTiter96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS) kit (Promega) reagent for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: Caspase-3/7 activity in leukemia cells were detected by Caspase-Glo 3/7 Assay System (G8091; Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then unfixed overnight in 150 mM NaCL and 60C and 50 ug/mL protease K (Promega). DNA was then extracted using a single column of QIAamp DNA Blood Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... inner salt] assay using CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS) kit (Promega, Cat# G3582) at 72 h after BKIDCs were added for all cell lines ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 8 µg of pcDNA3.1-GFP-mNLS-K145Q using Fugene 6 Transfection reagent (Promega, E2692) per manufacturer protocol ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from cell pellets using the Wizard Genomic DNA Extraction Kit (Promega UK, Catalogue number: A1120). For S ...
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bioRxiv - Biochemistry 2024Quote: ... 30 µg of proteins from lysed cells were digested with 500 units of endoglycosidase H (ENDO H, Promega) and 10 units of Peptide N-glycosidase F (PNGase F ...
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bioRxiv - Biochemistry 2024Quote: The amount of intracellular Glu was measured in 1×106 cells using the kit Glutamate-Glo (J7021; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Cells transduced with MCP-HALOnls were incubated for 24 hours before overnight staining with HaloTag-JF646 (Promega, #GA1120) at 10 μM ...
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bioRxiv - Microbiology 2023Quote: ... The samples were subjected to mechanical cell disruption by bead beating using EZ-Beads (Promega, Madison, WI, USA), followed by DNA purification using a Maxwell RSC Blood DNA kit (Promega).
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bioRxiv - Microbiology 2023Quote: Lentiviral particles were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DF1 cells were transfected with the different RCAS retroviral plasmids using FuGENE 6 Transfection reagent (Promega, Cat. E2691), accordingly to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were lysed for enzymatic cAMP measurement assay using a commercial kit and following manufacturer’s instructions (Promega, #V1501).
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bioRxiv - Microbiology 2023Quote: Transfection of DNA plasmids into different mammalian cells was conducted using FuGENE® HD (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were solubilized with 10 μL of 8M urea and 10 μL of 1% Protease MAX (Promega) in 100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then lysed and NanoLuc activity measured using the NanoGlo Luciferase Assay System (Promega, Madison, WI, USA). Luciferase activity was normalized to the number of GFP positive cells in each well.
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected the next day with a mixture of TP1-firefly luciferase and pRL-TK (Promega) plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined after 72 h using the CellTiter-Glo® luminescent assay (Promega, catalog number G9242). Cell viability of DMSO-treated cells was set to 100% ...
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bioRxiv - Genomics 2023Quote: ... Cells were lysed 24 h after transfection and assayed with the Dual-Luciferase Reporter assay kit (Promega, E1960).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Relative cell numbers were compared by measuring the luminescence signal generated by ATP using the CellTiter-Glo (Promega) assay ...
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bioRxiv - Molecular Biology 2023Quote: Firefly and Renilla luciferase activity was measured in cell lysates using the Dual-Luciferase Reporter assay system (Promega) in a GloMax navigator luminometer (Promega) ...
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bioRxiv - Systems Biology 2023Quote: Genomic DNA was extracted from each cell pellet using the Promega Wizard Genomic DNA Purification Kit (Promega #A1120), following standard protocol ...
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bioRxiv - Biochemistry 2023Quote: ... GFP intensity of cells treated with each drug library was measured by the fluorescence microreader (GloMax Discover, Promega). The inhibition rate for GFP intensity was calculated ...
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bioRxiv - Cell Biology 2023Quote: The supernatants of cells were collected and then applied to cytotoxicity test using CytoTox 96® Reagent (Promega) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... and 20 μl of the cell extract was analyzed using Dual-Luciferase Reporter Assay system (Promega, Madison, WI). Each experiment was carried out in triplicate ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed with 2× phosphate-buffered saline and lysed with 75 µL of passive lysis buffer (Promega), and then subjected to luciferase assay ...
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bioRxiv - Immunology 2023Quote: ... cells were washed 3X with prewarmed HBSS and fluorescence was read on GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Genomics 2022Quote: ... HepG2 cells were seeded in 24-well plates at a density of 100,000 cells/well and transfected with 400 ng pGL4.23-enhancer vector + 40 ng pRL-TK renilla vector (Promega) with Lipofectamine 3000 reagent ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were extracted with 1XPLB solution provided in the Dual-Luciferase® Reporter Assay Kit (Promega CN: E1910) and transferred to 96-well black plate to be treated with LARII and Stop & Glo components according to protocol of the Dual-Luciferase® Reporter Assay Kit (Promega CN ...
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bioRxiv - Cancer Biology 2023Quote: Isolation of genomic DNA from cultured cells was performed by Wizard genomic DNA isolation kit (Promega, WI, USA). Amplification of the barcodes was carried out by nested PCR ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was then isolated from the isopropanol/chloroform solution using the ReliaPrep RNA Cell Miniprep kit (Promega Z6010). For linear RNA depletion ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were then resuspended to an OD600 of 50.0 in lysis buffer (1x FastBreak cell lysis reagent (Promega), 50 μg/mL lysozyme ...
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bioRxiv - Bioengineering 2023Quote: ... CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) and CellTiter Blue were purchased from Promega (Madison, WI). Annexin V-Orange (4759 ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK cells were transfected with both heavy and light chain BCR constructs using FuGENE HD transfection reagent (Promega) in a 1:3 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... Subsequent measurements were taken every 24 hrs for 5 days using CellTiterGlo Luminescent Cell Viability Assay kit (Promega) and SpectraMax plate reader (Molecular Devices ...
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bioRxiv - Immunology 2023Quote: ... and IRF3-5D luciferase assays cells were lysed 20 hours post transfection in 1x passive lysis buffer (Promega). Luciferase activity was measured with the dual luciferase assay system (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase gene expression in E0771 and RM-1 cells was validated using a luciferase activity assay kit (Promega). Luciferase-labeled E0771 and RM-1 cells were maintained in RPMI 1640 media supplemented with 10% FBS ...