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Citations for Promega :
4251 - 4300 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Cells were transfected with CRISPR–Cas9 and donor plasmids (Table 2) using FuGENE HD Transfection Reagent (Promega, #E2311) in a 6-well plate following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... After 24 hours luciferase activities were assayed in cell lysates using the Dual-Luciferase Reporter Assay System (Promega) and a multi-label counter (Promega GloMax) ...
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bioRxiv - Cell Biology 2022Quote: For plasmid transfections cells were grown to 40-50% confluency and transfections were performed using FuGene6 (Promega, E2692) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were harvested after 48 hrs and luciferase assay was performed using the dual reporter kit (Promega) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... discarded the supernatant medium and lysed the cells using 1x PLB buffer in the Luciferase reporter kit (Promega) for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Then cells from each tube were lysed with 60 μL of Glo Lysis Buffer 1X (Promega, Cat.# E2661) and were vortexed for 5 sec ...
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bioRxiv - Biophysics 2022Quote: The viability and metabolic activity of SH-5YSY cells were verified by using an MTS colorimetric test (Promega). Cells were cultured in 24-well plates in 1 ml of the culture medium (DMEM) ...
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bioRxiv - Immunology 2021Quote: ... Media was then aspirated from cells and Firefly luciferase activity was determined with the Luciferase Assay System (Promega) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: Assays for proteasome activity were performed using the Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay Kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The cells were collected and assayed for reporter gene activities using the Dual-luciferase reporter assay system (Promega). The firefly/renilla luciferase ratios were calculated to determine luciferase activities ...
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bioRxiv - Cancer Biology 2019Quote: Cell proliferation assay was performed in 96 well plate using CellTiter 96® AQueous One Solution Reagent (Promega) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... After 36h of incubation cells were lysed and assayed for luciferase activities with a dual luciferase system (Promega). Plasmid pRLTK (Renilla ...
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bioRxiv - Immunology 2019Quote: ... NF-κB activity experiments were conducted using βTC3 cells transfected with 0.3 μg of the NF-κB.Luc reporter (Promega) and 0.25 μg CMV.β-galactosidase (a kind gift from Beth Israel Harvard Medical School ...
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bioRxiv - Neuroscience 2019Quote: ... The impact of compounds on H4 cell toxicity was assayed using the CytoTox-Glo kit (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2019Quote: ... the number of viable cells was determined by MTS assay or CellTiter-Glo assay (Promega, Madison, WI, USA) following the kit protocol ...
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bioRxiv - Cancer Biology 2019Quote: 293T cells were transfected with pSpCas9(BB)–2A–Puro:sgRNA #4 (0.5 μg) using the FuGENE HD Transfection Reagent (Promega). Two days after transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... After 48 h cells were lysed and luciferase activity was measured using the Dual-Luciferase Assay System (Promega). All luciferase data were corrected for transfection efficiency based on the Renilla internal control following by subtraction of the pGL4.10[luc2] activity background ...
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bioRxiv - Biochemistry 2019Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... the cells were transfected with different luciferase vectors (100ng) along with 0.5ng renilla vector (pGL4.74, [hRluc/TK], Promega) using Lipofectamine 3000 reagent (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then transfected with 1500 ng pcDNA-FLAG-HA-sscGAS plasmid complexed with FuGene 6 reagent (Promega) according to manufacturer’s instructions or treated with FuGene 6 alone as a negative control ...
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bioRxiv - Genomics 2020Quote: ... from 106 cells and treated with DNAase (20mM MgCl2 [Invitrogen], 2mM DTT [Sigma-Aldrich], 0.4U/uL RNasin [Promega] ...
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bioRxiv - Genetics 2019Quote: ... Cells were harvested after 48 hours and the luciferase activity measured by Dual-Glo Luciferase Assay system (Promega). For each sample ...
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bioRxiv - Microbiology 2021Quote: ... cells were harvested for Nanoluc luciferase assays using the Nano-Glo® Luciferase Assay System (Promega, Cat# N1150).
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bioRxiv - Microbiology 2021Quote: ... CytoTox-One Homogeneous Membrane Integrity Assay was used to measure potential cell toxicity according to manufacturer’s instructions (Promega).
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bioRxiv - Neuroscience 2020Quote: ... pCAG-DCC:TDTOMATO and pCAG-DCCkanga:TDTOMATO plasmids (1 µg) were transfected into the plated U251 cells using FuGENE 6 (Promega) in Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed and luciferase assays were performed using the Dual-Luciferase® Reporter Assay System (Promega, E1980) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then lysed and luciferase activity assessed using the ONE-Glo™ EX Luciferase Assay System (Promega) according to the manufacturer’s specifications ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then lysed and luciferase activity assessed using the ONE-Glo™ EX Luciferase Assay System (Promega) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected at 24 h after the induction of differentiation and dissolved in Passive Lysis Buffer (Promega). The effect of hnRNPK on myogenin promoter was measured using a Lumat LB 9507 luminometer (Berthold Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The identity of all cell lines was confirmed by DNA fingerprinting analysis utilizing the GenePrint 10 System (Promega).
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bioRxiv - Cancer Biology 2020Quote: ... cells were transiently transfected with 0.1 ug of an individual plasmid with Fugene HD reagent (Promega, Madison, WI). 48 hours later ...
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bioRxiv - Cancer Biology 2020Quote: ... cell extracts were obtained and assayed for luciferase activity by using the Dual-Luciferase assay kit (Promega, #E1910), as specified by the manufacturer ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA from all screens was extracted from cell pellets using the Wizard Genomic DNA Purification kit (Promega). Sequencing libraries were prepared from 100 μg of gDNA for control sample and non-cytopathic screen samples or 10 μg of gDNA for cytopathic screen samples via a 2-step nested PCR using primers that include Illumina TruSeq adapters with i5 and i7 indices ...
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bioRxiv - Microbiology 2021Quote: ... Transduced cells were lysed 48h later and luciferase activity was measured by using the luciferase assay system (Promega).
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bioRxiv - Microbiology 2021Quote: ... cells were assayed for NanoLuc activity using Nano-Glo Luciferase Assay System or Luciferase Assay System (E1501, Promega).
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bioRxiv - Microbiology 2021Quote: ... cells were lysed and luciferase activity was determined using the Renilla luciferase Assay System (Promega, Leiden, The Netherlands). Since most of the mutations abrogated virus replication ...
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bioRxiv - Molecular Biology 2021Quote: ... Low passage MCF10A cells were transfected at 80% confluency in a 25cm2 flask using 25 μl FugeneHD (Promega) with 6 μg of pAS4863 and 200 ng of GBlock donor template ...
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bioRxiv - Microbiology 2021Quote: ... and 800 ng pSPAX2 were co-transfected into HEK 293T cells using the FuGENE HD Transfection Reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Cellular viability was determined using non-radioactive CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) reagent (Promega), according to the manufacturer’s recommendations29 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 hours after plating cells were transfected with 1μg of histone H3.3-HaloTag®Fusion Vector DNA (Promega) + 0.1 μg of NSD2 PWWP1-NanoLuc® Fusion Vector DNA (C-terminal ...
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bioRxiv - Microbiology 2020Quote: ... intracellular ATP was quantified by using the BacTiter-Glo Microbial Cell Viability Assay Kit (Promega CAT No.: G8230). Aliquots of 100 µl of bacterial culture were collected at various time points and mixed with an equal volume of the BacTiter-Glo reagent and incubated for 5 min in the dark ...
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bioRxiv - Microbiology 2019Quote: ... 48 hours later cells were transfected with a plasmid encoding HA of the FPV mutant using Fugene (Promega). 24 hours later cells were labelled for 2 hr with 200 µCi/ml [3H]-palmitic acid (9,10-3H(N) ...
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bioRxiv - Neuroscience 2020Quote: ... The impact of compounds on H4 cell toxicity was assayed using the CytoTox-Glo kit (Promega, Madison, WI).
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bioRxiv - Neuroscience 2020Quote: ... The impact of compounds on H4 cell toxicity was assayed using the CytoTox-Glo kit (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2021Quote: The cytotoxicity of compounds was determined by using the ATP-based CellTiter-Glo Luminescent Cell Viability Assay (Promega) and analyzed by the Cytation 5 multimode reader (luminescent mode) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were co-transfected with luciferase-containing constructs (pGL3) and phRL-TK synthetic renilla vector (Promega, Madison, WI) in a molar ratio of 1:60 (phRL-TK versus pGL3) ...
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bioRxiv - Cancer Biology 2020Quote: ... and cell lysates were prepared and examined by using the Dual luciferase reporter assay system (Promega, Madison, WI), according to the manufacturer’s protocol.
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... intracellular ATP levels were measured using CellTiter-Glo® Luminescent Cell Viability Assay Kit (Promega, Madison, WI, USA), at room temperature and according to the vendor’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: HeLa cells were transfected with pSpCas9(BB)-2A-GFP encoding gRNAs using FuGENE HD Transfection Reagent (E2311; Promega). Two days after transfection ...