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Citations for Promega :
501 - 550 of 1059 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... We used the resulting cDNA to perform quantitative PCR with GoTaq master mix (Promega), and analyzed data with the standard ΔΔCt method ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega), and used directly for quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2021Quote: ... and cDNA was generated using GoScript Reverse Transcription System following the manufacturers protocol (Promega). Viral RNA was detected using the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel as per Centre for Disease and Control’s optimised protocol (Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... mRNA levels were quantified in cDNA by qPCR with GoTaq qPCR Master Mix (Promega) according to supplier’s instructions in a Mx3000 Real-Time Thermocycler (Stratagene ...
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bioRxiv - Immunology 2022Quote: ... Quantity and quality of cDNA was assessed with the quantifluor RNA system (Promega E3310) and Agilent 2100 BioAnalyzer with a high-sensitivity chip ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was prepared using ‘ImProm-II™ Reverse Transcription System’ (Promega cat No: A3800) according to the kit protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription-PCR was performed using a cDNA synthesis kit (Promega, Madison, WI, USA) and 2 μg of total RNA according to the manufacturer's protocol ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was reverse transcribed into cDNA using the GoScriptTM Reverse Transcription System (Promega). Quantitative real-time polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was prepared using ImProm-II™ reverse transcription kit (Promega, A3800) and 10 ng of cDNA were used as template for each real-time PCR reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... This cDNA was then used for PCR with GoTaq G2 Flexi Polymerase (Promega, M7801), using a forward primer annealing to the 3’ UTR of the mRNA of interest (all primer sequences in Supplementary Table 5 ...
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bioRxiv - Cell Biology 2019Quote: The cDNA were amplified using a GoTaq®qPCR master mix (Promega, Charbonnieres, France) or a FastSart Universal SYBR Green master mix (containing Rox ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesis was carried out using the GoScript Reverse Transcription system (Promega, USA) using OligoDT ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated from the RNA using a GoScript™ Reverse Transcription System (Promega) according to the vendor’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 2ug of total RNA using M-MLV reverse transcriptase (Promega) with an oligo(dT)18-25 primer ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized at 42 °C for 60 min with M-MLV-RT (Promega) using gene-specific primer SP1_nbdA ...
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Ion transport modulators differentially modulate inflammatory responses in THP-1 derived macrophagesbioRxiv - Immunology 2021Quote: ... after which cDNA was synthesised using the GoScript reverse transcription system (Promega, Madison, USA). Quantitative PCR was performed using the Mesa green qPCR master mix (Eurogentec ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA synthesis was performed by the ImProm-II™ Reverse Transcription System (Promega) and oligo(dT) ...
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bioRxiv - Microbiology 2021Quote: ... Afterwards RNA was converted into cDNA using a GoScript™ Reverse Transcription kit (Promega), and stored at −20°C until analysis ...
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bioRxiv - Microbiology 2021Quote: ... We synthesized cDNA from 2 μL of RNA using M-MLV reverse transcriptase (Promega) and random hexamers ...
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bioRxiv - Microbiology 2021Quote: ... and cDNA synthesised from 500 ng total RNA using M-MLV Reverse Transcriptase (Promega). Mouse or human Xbp1 and Rpl19 were amplified using specific primers (Supplementary Table 5) ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA synthesis was carried out using random hexamers and Improm-II reverse transcriptase (Promega). The resulting cDNA was used for qPCR via iQ SYBR Green Supermix (Bio-Rad) ...
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using random hexamers and ImProm-II™ Reverse Transcriptase (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Genomics 2021Quote: ... cDNA was synthesized from 1 μg RNA using M-MLV reverse transcriptase (9PIM170, Promega) and Oligo(dT)15 primers (C110A ...
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bioRxiv - Physiology 2021Quote: ... RNA was reverse transcribed into cDNA using the ImProm-IITM Reverse Transcription System (Promega) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... RNA (2 μg) was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega). Conventional PCR was used with the primer (Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR on cDNA or ChIP DNA was performed with GoTaq qPCR Master Mix (Promega) under the manufacturer’s instruction on a QS5 system (Thermo Scientific) ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... and cDNA was synthesized using the Reverse transcriptase kit (Promega Benelux, Leiden, The Netherlands) according to manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... RNA was transcribed into cDNA using random hexamers and goScript MMLV reverse transcriptase (Promega) following the manufacturers’ instructions.
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bioRxiv - Genetics 2020Quote: ... The first cDNA strand was synthesised from RNA using M-MLV Reverse Transcriptase (Promega) with both random hexamer and oligo(dT ...
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bioRxiv - Molecular Biology 2022Quote: ... The first-strand cDNA was prepared by GoScriptTM Reverse Transcription Mix (Promega, Madison, WI) using 1 μg of extracted RNA of C ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was reverse transcribed to cDNA using MMLV reverse transcriptase (Promega, Wisconsin, USA). RT-qPCRs were performed using SYBRGreen mix (BioRad iQ supermix) ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2022Quote: ... and first-strand complementary DNA (cDNA) was synthesized using ImProm-II reverse transcriptase (Promega). Quantitative Reverse Transcription PCR analysis was performed using a CFX96 Real-Time System (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: cDNA was prepared from the isolated RNA using M-MLV Reverse Transcriptase (Promega, USA) as in Petrasch et al ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 1 µg of RNA using M-MLV Reverse Transcriptase (Promega) and oligo d(T)15 following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and cDNA was synthesized using the M-MLV Reverse Transcriptase (Promega, Leiden, the Netherlands). Real-time PCR was conducted using a C1000 Touch™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... The size of the amplified cDNA was selected using ProNex beads (Promega, Madison, WI) under standard conditions ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was generated from 2 µg isolated RNA using M-MLV reverse transcriptase (Promega) and diluted 1:15 for use in 12 µL qPCR reactions run on the StepOnePlus™ system (Applied Biosystem) ...
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bioRxiv - Developmental Biology 2023Quote: ... Concentration and fragment size of amplified cDNA was measured with QuantiFluor® dsDNA (Promega) and Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Cell Biology 2022Quote: ... and 1-2 µg of RNA was reverse transcribed into cDNA using GoScript (Promega).
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bioRxiv - Cell Biology 2023Quote: ... The first-strand cDNA was converted using a reverse transcription system (Promega, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was synthesized from each reaction mixture using the GoScript Reverse Transcription System (Promega) per the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... cDNA synthesis was conducted by using the ImProm□IITM Reverse Transcription System (#A3800, Promega), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RNA (3μg) was converted to first strand cDNA using reverse transcriptase (Promega, United States) and oligo-dT ...
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bioRxiv - Plant Biology 2023Quote: ... followed by reverse-transcription into first-strand cDNA using ImProm II reverse transcriptase (Promega), anchored oligo(dN-T20 ...
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bioRxiv - Molecular Biology 2023Quote: ... was used as template to synthesize cDNA with M-MLV reverse transcriptase (Promega, M1701). Each quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... equal amounts of (strand specific) cDNA was used with Ready Go Taq Polymerase (Promega). To ensure specific DNA was amplified ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized by the ImProm-II™ Reverse Transcriptase system (Promega, Cat# A3801) using 100 ng RNA per reaction ...