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Citations for Promega :
1701 - 1750 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and the resulting PCR product (3918bp) was cloned into pGEM®-T Easy Vector (Promega). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons were purified with the Wizard SV Gel and PCR Clean-Up system (Promega Switzerland).
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bioRxiv - Genetics 2022Quote: ... The digested minigenes were purified with Wizard SV Gel and PCR Clean-UP System (Promega) and ligated into a BamHI/MluI opened pCAS2 splicing reporter minigene vector (kindly provided by Dr ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons were purified using a Wizard SV Gel and PCR Clean up System kit (Promega) and EcoRI/XhoI digested pGREG533 was gel purified using a Qiaex II kit (Qiagen) ...
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bioRxiv - Neuroscience 2019Quote: Single cell PCRs were performed on diluted cDNA (1:100) using Go-Taq polymerase (Promega) with the following protocol ...
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bioRxiv - Plant Biology 2019Quote: ... All PCR reactions were carried out using Go Taq DNA Polymerase (Promega, Madison, WI, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Flnb and Myocd were PCR amplified and cloned into HindIII/EcoRI sites of pGEM4Z (Promega) for in vitro transcription ...
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bioRxiv - Genomics 2019Quote: Candidate TRs with long alleles identified in NA12878 were PCR amplified using GoTaq (Promega #PRM7123) with primers shown in Supplementary Table 5 ...
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bioRxiv - Neuroscience 2019Quote: ... The purified PCR product was subcloned into the pGEM-T Easy vector (Promega, Madison, WI) for Sanger sequencing ...
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Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylatebioRxiv - Biochemistry 2019Quote: ... Reactions were quenched and deproteinized by PCR Clean-Up System (Promega, Madison, Wisconsin, United States). 10 μl eluted product was incubated with T4 DNA Ligase (New England BioLabs ...
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bioRxiv - Biophysics 2019Quote: ... or the Wizard® SV Gel and PCR Clean-Up System (Promega GmbH, Mannheim, Germany). All primers were obtained from biomers.net ...
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bioRxiv - Cell Biology 2020Quote: ... PCR fragments were gel extracted and cloned with the pGEM-T easy Vector system (Promega) and transfected into XL10Gold competent cells (Agilent) ...
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bioRxiv - Molecular Biology 2020Quote: ... DHRS2 were amplified by PCR from genomic DNA and subcloned into pNL3.1-minP/Nluc (Promega). Cells were transfected with the normalization plasmid (pGL4.54-Luc2/TK) ...
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bioRxiv - Genomics 2020Quote: ... PCR reactions were run according to the GoTaq® G2 Flexi DNA polymerase instructions (Promega), with 50 ng of template DNA ...
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bioRxiv - Systems Biology 2019Quote: ... Half of the cDNA (5 μl) was amplified by PCR using Pfu Polymerase (Promega, M7745) with the cycling conditions (95°C for 2 min ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were cloned downstream of Renilla luciferase of psiCHECK(TM)-2 vector (Promega) using XhoI and NotI sites.
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Biochemistry 2019Quote: ... Equal amounts of cDNA were used in PCR reactions with Ready Go Taq Polymerase (Promega). A minus-RT control was used to ensure no contaminating genomic DNA was amplified ...
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bioRxiv - Bioengineering 2020Quote: ... The first PCR products were purified using a commercial purification kit (Promega, Madison, WI, USA), diluted ...
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bioRxiv - Genetics 2021Quote: ... The purified DNA was PCR amplified using GoTaq Colorless Master Mix (Promega, Madison, WI, USA), and the PCR product was extracted as above to eliminate primer-dimers ...
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bioRxiv - Microbiology 2020Quote: ... BRYT Green dye-based quantitative PCR (qPCR) was performed with GoTaq qPCR Master Mix (Promega) and intron-spanning primer pairs (see Suppl ...
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bioRxiv - Genomics 2019Quote: ... Quantitative PCR was performed in 3 technical replicates with the GoTaq qPCR Master Mix (Promega) in 384 well plates ...
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bioRxiv - Microbiology 2020Quote: ... End-modified PCR products were ligated into pGEM-T Easy vector (Promega, Madison, WI, USA). The vector-insert construct was used to transform chemically competent DH5α cells or OneShot Top 10 E ...
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bioRxiv - Immunology 2021Quote: ... quantitative real-time PCR was carried out using the GoTaq qPCR Master Mix kit (Promega) with 20 µL of reaction mixture containing gene-specific primers or the PrimePCR assay kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... the PCR product was purified and cloned in pGem-T Easy Vector Systems (Promega Corporation) as per the vendor’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR reaction mix used was: 10 μL GoTaq Green Master Mix (Promega, Madison, WI), 1 μL RRVP3-F3 and RRVP3-B3 or RRVP4-F3 and RRVP4-B3 primers (5 μM) ...
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bioRxiv - Immunology 2021Quote: ... PCR products were purified and transcribed in vitro using T7 RNA polymerase (RiboMAX system, Promega). All transcripts were translated in rabbit reticulocyte lysate (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR was performed in a BioRad C1000 Touch thermocycler using GoTaq mix (Promega #A6001) [32] ...
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bioRxiv - Biophysics 2020Quote: ... This plasmid was used as a template for PCR (Pfu DNA polymerase; Promega, Madison, WI) using megaprimers consisting of 30 to 45-nucleotide homology flanking the altered nucleotides ...
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bioRxiv - Microbiology 2021Quote: ... and the PCR product from this reaction was cloned into pGEM T-Easy vector (Promega), transformed into competent DH5-alpha E ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplified PCR products were cloned into pGEM-T easy vector (Promega, Madison, WI, USA). Sequencing for recombinant clones (n = 6 ...
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bioRxiv - Microbiology 2022Quote: ... PCR was carried out individually for each gene using GoTaq® Green Master Mix (Promega), with primers listed in Table 1 ...
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bioRxiv - Genetics 2022Quote: ... Purified PCR products were TA-cloned using the pGEM-T Easy Vector System (Promega, A1360), according to the manual instructions ...
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bioRxiv - Microbiology 2022Quote: ... Resulting cDNAs were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and tailing with dATP and TdT ...
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bioRxiv - Microbiology 2022Quote: ... gel purified using a Wizard® SV gel and PCR clean up system (Promega, Australia) and sequenced using an Illumina MiSeq platform at the Ramaciotti Centre for Genomics ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was carried out as per the manufacturer’s guidelines for GoTaq G2 Polymerase (Promega, M784B) with an annealing temperature of 58°C and a 1 minute extension time.
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bioRxiv - Microbiology 2022Quote: ... individual rough colonies were used to conduct colony-PCR using GoTaq Green master mix (Promega) and a primer set unique to different regions on each of the BcG9241 plasmids pBCX01 and pBC210 ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoresis revealed a single band that was PCR purified and cloned using pGemT-easy (Promega). Sequencing was performed at the University of Michigan DNA sequencing core ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR solution for each sample contained 12.5 µL GoTaq Green Master Mix (Promega, M7123), 1.5 µL common forward primer ...
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bioRxiv - Microbiology 2022Quote: ... The purified PCR product was cloned into the pGEM-T vector (Promega, Fitchburg, WI, USA), and later into the pGAPzαA vector (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative PCR (qPCR) reactions included 10 μl 1 X GoTaq® qPCR Master Mix (Promega), 0.5 μM of each primer and 5 μl template cDNA ...
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bioRxiv - Developmental Biology 2023Quote: ... then DNA was purified using the Wizard SV Gel and PCR Clean-Up System (Promega) according to manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified with the Wizard SV Gel and PCR clean up System (Promega, Madison, USA). PCR products were cloned into pGem-T Easy vectors (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... Gel-purified PCR fragments were cloned into the pGEM-T Easy vector (Promega, cat# A137A) and sequenced using the Sanger’s method.
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bioRxiv - Cell Biology 2023Quote: ... Sequencing of the gRNA-targeted exon 1 was performed by PCR amplification (Promega GoTaq polymerase) with 5’-GGTTTGGGGCTGGGCAT-3’ and 5’-AGGTGCAGCAGCAGTACG-3’ primers (Guillen-Samander et al. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Multiplex PCRs were modified using 5X GoTaq Flexi colorless reaction buffer (Promega Corp., Madison, USA) instead of 1X and performed using a T100™ Thermal Cycler (Bio-Rad Laboratories Inc.).
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bioRxiv - Biochemistry 2023Quote: ... the linearized DNA was purified using the Wizard® SV Gel PCR purification kit (Promega). For the synthesis of 5’-capped mRNA ...
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bioRxiv - Microbiology 2023Quote: ... cDNA amount was assessed by quantitative PCR (qPCR) using GoTaq qPCR Master Mix (Promega, USA), 0.4 μM of each primer ...
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bioRxiv - Plant Biology 2023Quote: ... Products were cleaned with the Wizard® SV Gel and PCR Clean-Up System (Promega) and cloned with the pGEM®-T Vector Systems (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... Individuals of the F2 progeny were analysed by PCR genotyping using GoTaq2 DNA polymerase (Promega) according to standard protocols ...