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Citations for Promega :
1751 - 1800 of 5637 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... followed by polymerase chain reaction (PCR) with GoTaq® G2 Master Mix (M7822, Promega) on Biometra TRIO Touch Thermocycler (207072X ...
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bioRxiv - Molecular Biology 2023Quote: ... by PCR and cloned into the Halo-tagged pFN21A vector (Promega, Madison, WI, USA). The AMPKγ1 mutant vector wherein Ser260 and Thr262 were replaced with alanine was created using parent-template backbone mouse AMPKγ1- full-length vector (# 15996 ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction mixture for all reactions consisted of 2x GoTaq Green Mastermix (Promega), 400nM primer-F ...
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bioRxiv - Genomics 2023Quote: The target cut sites were amplified by PCR using GoTaq G2 Green (Promega,# 9PIM782). Similar to Reding et al ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed using a GoTaq qPCR master mix per manufacturer’s instructions (Promega). All gene expression levels were normalized against that of GAPDH.
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bioRxiv - Biophysics 2022Quote: ... which was replaced by PCR-amplified LgBit insert from LgBit Expression Vector (Promega#N2681). AgeI and SpeI restriction enzymes (NEB ...
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bioRxiv - Immunology 2023Quote: ... Unpurified PCR product was used as input to rabbit reticulocyte transcription translation system (Promega) and Nano-Glo HiBit Lytic Detection System (Promega Cat No ...
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bioRxiv - Cancer Biology 2023Quote: ... The amplified PCR products were isolated and subcloned into pGEM-T easy vector (Promega) and sequenced using Bigdye terminator v3.1 (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR product was cloned using the pGEM®-T Easy vector (Promega, USA) before the sequencing ...
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bioRxiv - Genetics 2023Quote: ... and amplified by PCR using the GoTaq Master Mix (Promega, Charbonnières les Bains, France). We employed specific primers for PTGER1 ...
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bioRxiv - Microbiology 2023Quote: The amplified products underwent purification using Quick PCR purification columns (Promega, Madison, WI, USA) and were subsequently sequenced with the Big Dye Terminator Cycle Sequencing Ready Reaction Kit on an Applied Biosystems analyzer (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR products were digested by direct addition of 0.5 µl of HindIII (Promega) and incubation for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were amplified by 16 cycles of PCR with GoTaq Flexi DNA Polymerase (Promega), purified using Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR-amplified products were then cloned into the pGEM-T Easy Vector (Promega) and subjected to sequencing for further analysis.
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bioRxiv - Molecular Biology 2023Quote: ... Parental plasmids were selectively linearised from the PCR product via digestion with Dpnl (Promega) in lx MULTI-CORE buffer for 1hr at 37°C and the digestion product was immediately transformed into Stbl3 competent bacteria.
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bioRxiv - Biochemistry 2023Quote: Quantitative PCR: Taq qPCR was performed using SYBR Green GoTaq qPCR master mix (Promega) according to manufacturer’s instructions on a LightCycler 96 SW 1.0 (Roche Diagnostics GmbH ...
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bioRxiv - Biophysics 2023Quote: ... a regular PCR was run on a thermocycler using Taq polymerase master mix (Promega). Gene knockout was thereby confirmed by gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic sites encompassing targeted guide regions were amplified by PCR using GoTaq polymerase (Promega) and sequenced ...
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bioRxiv - Immunology 2024Quote: ... the Renilla luciferase sequence was PCR amplified from plasmid pRL-TK-Renilla (Promega, E2241) and the hPEST sequence was PCR amplified from plasmid pcDNA3.1(+)GUG-nLuc-3XFLAG-CL1/PEST (Addgene plasmid #127317 ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR was performed using a GoTaq qPCR master mix per manufacturer’s instructions (Promega). All gene expression levels were normalized against that of GAPDH.
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bioRxiv - Cancer Biology 2024Quote: ... The PCR product was gel-purified and cloned into the pGL3-control plasmid (Promega) at the XbaI and SalI sites ...
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bioRxiv - Microbiology 2024Quote: ... Each 25 μl PCR reaction was prepared using GoTaq G2 Flexi DNA Polymerase (Promega) and contained 0.5 U μl−1 GoTaq G2 Flexi DNA Polymerase ...
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bioRxiv - Neuroscience 2024Quote: ... and PCR was performed with GoTaq G2 Flexi DNA Polymerase (Promega, catalogue no. M7805) with primers to amplify DNA sequence around the sgRNA targeting sites within the gene of interest (Supplementary Table 6) ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was cleaned up with Promega Wizard SV gel and PCR Cleanup System (Promega). The inserts and the backbone were mixed in a 3:1 ratio and incubated with the 2x NEBuilder HiFi DNA assembly enzyme mix (New England Biolabs ...
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bioRxiv - Physiology 2024Quote: ... the PCR products were ligated into the pGEM-T Easy Vector (Promega, Madison, WI), and the sequences were confirmed by Sanger sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were purified and cloned into the pGEMT vector (Promega, Madison, WI, USA). A minimum of 10 clones per PCR product were then sequenced by the Sanger method to characterize the edited alleles.
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bioRxiv - Plant Biology 2024Quote: ... Genes of interest were amplified from template DNA by PCR using GOTaq polymerase (Promega) according to the recommendations of the supplier ...
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bioRxiv - Cell Biology 2020Quote: ... Cell growth and viability were measured on the 96 well white bottom plate as an end point measurement for each time point and/or treatment using the Cell-titer GLO assay (Promega, # G7570) based on manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with 100 μl/well of PBST and 100 μl/well of 3,3,′5,5-tetramethylbenzidine TMB (Promega, Madison, WI, USA) were added and incubated for 20 min at RT ...
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bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... At the indicated time points firefly and Renilla luciferase activities were measured using the Dual-Glo® Luciferase assay system (Promega) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... washing two times with 100 µl PBS and then lysed with 1x lysis buffer (made from 5x Promega Firefly Lysis Buffer). Plates were frozen for at least 24 hours and then thawed for 2 hours before analyzing with Promega Firefly Luciferase Kit on a luminometer.
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bioRxiv - Cell Biology 2019Quote: ... non-fluorescent blocking ligand was washed out and cells were additionally washed three times with fresh DMEM medium and incubated DMEM medium containing Janelia Fluor 646 (JF646) HaloTag ligand (Promega, GA1121) in final labeling concentration 200 nM for indicated timepoints.
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bioRxiv - Cancer Biology 2021Quote: ... Plates were then incubated at 37°C in 5% CO2 and cell viability was assessed at the indicated time points by measuring ATP content through Cell Titer-Glo® Luminescent Cell Viability assay (Promega) over 22 and 32 days of constant treatment (for WiDr and DiFi respectively) ...
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bioRxiv - Microbiology 2022Quote: ... the beads were washed three times and bound NanoLuc activity was measured using Nano-Glo Luciferase Assay System and a GloMax® Navigator Microplate Luminometer (Promega). To construct standard calibration curves for measurement of mAb levels in plasma ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were diluted 8 times with 0.1M ammonium bicarbonate and sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) was added ...
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bioRxiv - Microbiology 2022Quote: ... the beads were washed three times and bound NanoLuc activity measured using Nano-Glo Luciferase Assay System and a GloMax® Navigator Microplate Luminometer (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was washed three times for ten minutes and then incubated for 5 minutes with an enhanced chemiluminescence reagent (Promega W1001). The blot was imaged in an Azure Biosystems C600 imaging system ...
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bioRxiv - Cell Biology 2024Quote: ... Protein-bound magnetic beads were washed three times with 200 µl of 80% ethanol and reconstituted in 50 mM TEAB and digested with trypsin (Promega, V5111) at a 1:50 enzyme-to-substrate ratio for 16 h at 37 °C with constant shaking (1000 rpm) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability of each sample in triplicates at various time points was measured using a luminescence assay of ATP (CellTiter-Glo 2.0 Assay, Promega, Cat. #G9242) following manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... FuGENE HD Transfection Reagent with a 20-minute complexing time was used to introduce the transgenes into the cells (Promega E2312). For stable cell lines ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 20 times with 50 mM ABC (no guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times in TBST for 15’ per wash and incubated 1 hour in HRP-conjugated anti-rabbit (Promega, W4011) or anti-mouse (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of protein was diluted 5 times with 50 mM ABC and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested 24 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 10 times with 50 mM ABC (no Guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The MTS assay was carried out at certain time points using The CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega #G3580) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 20 times with 50 mM ABC (no guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... caspase8 Kit (Promega company); Caspase3 / 7 Kit (Promega company).
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bioRxiv - Molecular Biology 2020Quote: ... or Maxwell kit (Promega). Following RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... the GoScript Kit (Promega) was used to make cDNA and qRT-PCR performed on a BioRAD c1000 Touch thermocycler.