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Citations for Promega :
1901 - 1950 of 5335 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... We selected the transformed T1 plants carrying the targeted insertion by PCR using GoTaq DNA polymerase (Promega). As T1 plants of Mpseta-1ko and Mpseta-2ko were both females ...
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bioRxiv - Biophysics 2019Quote: ... The generated PCR fragment was A-tailed and ligated into the PGEM-T Easy Vector System (Promega)21 ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products were transcribed in vitro using the RiboMAX Large Scale RNA Production System-T7 (Promega) at 37°C for 4 h ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the dCK gene was PCR-amplified from pDNR-Dual_dCK (DNAsu HsCD00000962) using Pfu DNA polymerase (Promega, Germany) and gene-specific primers (Eurofins Genomics ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 700 ng used for in vitro transcription/translation using TnT T7 Quick for PCR DNA (Promega) and the Fluorotect GreenLys tRNA (Promega ...
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bioRxiv - Molecular Biology 2019Quote: The entire genome was amplified by PCR using GoTaq 2× Hot Start Colorless Master Mix (Promega, USA) using overlapping primer sets (Ali et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was performed in 10 μL reactions comprising of 1X GoTaq® qPCR master mix (Promega), 1 μM of gene specific forward and reverse primers (sequences are provided in Supplementary Table 3 ...
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bioRxiv - Genomics 2021Quote: ... Diluted cDNA (1:20) was used in a PCR reaction with the GoTaq qPCR Master Mix (Promega) and run on a CFX384 Touch instrument (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... purified from agarose gels using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, USA) and sent for sequencing (to GATC Services-Eurofin Genomics ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested with the BamHI and Sall restriction enzymes and inserted into pGEM4Z (Promega) that had been digested with the same enzymes ...
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bioRxiv - Biophysics 2020Quote: ... 1 µL of gDNA was added to a standard 24 µL 2X GoTaq Green PCR (Promega, #M7123), typically ∼35 cycles ...
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bioRxiv - Biophysics 2021Quote: ... All DNA handles were made by polymerase chain reaction (PCR) using λ DNA cl857 Sam7 (Promega, D1501) as a template and a forward primer containing two digoxigenin labels at the 5’ end ...
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bioRxiv - Microbiology 2021Quote: ... Spike+ cells were then single cell sorted into 96-well PCR plates containing RNase inhibitor (N2611; Promega) and stored at −80 °C.
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bioRxiv - Microbiology 2020Quote: ... The concentration of PCR products was then measured using the QuantiFluor™ dsDNA System (Promega, Madison, WI) and used to pool equimolar amounts of PCR products together ...
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bioRxiv - Cell Biology 2021Quote: The qRT-PCR reaction was carried out with GoTaq®qPCR Master Mix (Promega, Madison, WI, USA) on a CFX96 instrument (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... DNA was resuspended in 30 uL PCR grade water and quantified by Quantus™ Fluorometer (Promega Inc). The PCR product pools were sequenced by short read sequencing as described by 2.3.1.
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bioRxiv - Developmental Biology 2022Quote: ... The amplified PCR fragments were subcloned into the pGEM T Vector system (Promega Corporation, Madison, WI, USA) and sequenced to confirm each allele.
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bioRxiv - Cell Biology 2022Quote: ... P2-2: -212- -826) were cloned through PCR into the pGL4.20 luciferase reporter vector (Promega, Madison, WI) using C57BL/6 genomic DNA as a template ...
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bioRxiv - Bioengineering 2022Quote: ... the EGFP coding sequence was PCR amplified from the plasmid pDestattB_ubi:EGFP using GoTaq® DNA Polymerase (Promega) with attB-P2A-EGFP-F and pA-r primers (Supplementary Table S3) ...
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bioRxiv - Cell Biology 2022Quote: ... and aliquots of the cells were analyzed by genomic PCR using GoTaq DNA polymerase (Promega, WI, USA) and specific primers (F_C2 ...
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bioRxiv - Physiology 2022Quote: ... the PCR products were subjected to T/A cloning (pGEM®-T Vector Systems; Promega, Fitchburg, USA) with subsequent Sanger sequencing (Sanger et al ...
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bioRxiv - Plant Biology 2023Quote: ... AP1 (AT1G69120) cDNA was PCR-amplified using specific primers and inserted into XbaI/BamHI digested pSP64 (Promega) vector ...
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bioRxiv - Microbiology 2023Quote: ... The 3809-bp PCR product was purified and cloned into pGEM-T-Easy (Promega, Medison WI, USA) to yield pGEM ...
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bioRxiv - Genetics 2023Quote: ... PCR was performed in a 30 µL reaction volume containing 1X Colorless GoTaq Reaction Buffer (Promega, #M792B), 200 µM dNTPs (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... All PCR analyses were performed on 0.5 ng cDNA using 0.5 U GoTaq® DNA polymerase (Promega). Quantification of PCR products was performed using the Image Lab software (BioRad ...
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bioRxiv - Genomics 2023Quote: ... Agar was removed from the DNA using the Wizard SV Gel and PCR Clean-Up System (Promega). Transposon-directed insertion site sequencing (TraDIS ...
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bioRxiv - Genetics 2022Quote: ... The digested plasmid was purified using the Wizard SV Gel and PCR Clean-Up System (Promega A9281). The zebrafish exorh promoter region was amplified from pCK029 (this manuscript ...
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bioRxiv - Genetics 2022Quote: Quantitative PCR was performed on the Roche LightCycler 96 instrument by using GoTaq qPCR Master Mix (Promega) with 1µl cDNA template and 0.5µl of 10mM each primer in a total volume of 25µl reaction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The amplified products were gel-purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and then cloned into the pGEM-T Easy Vector (Promega) ...
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bioRxiv - Genomics 2023Quote: ... PCR validation reactions amplification were performed using GoTaq® DNA polymerase (Promega, 1.2 units/50 μl reaction), Colorless GoTaq® Reaction Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and used 1 μL of cDNA for 10 μL of PCR using 2x GoTaq master mix (Promega) and 250 nM of forward and reverse primer sets with conditions recommended by the manufacturer ...
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bioRxiv - Physiology 2023Quote: ... Each 10 µl PCR reaction consisted of 5 µl of GoTaq® qPCR Master Mix (Promega Corporation), 0.5 µl of 10 µM forward primer ...
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bioRxiv - Developmental Biology 2023Quote: ... A-tailed PCR products were ligated with digested linear pGEM®-T Vector (Promega, Cat. No. A3600), followed by transformation with One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts and plasmid backbones were purified with Promega Wizard SV gel and PCR Cleanup System (Promega). Purified inserts and backbones were mixed in a molar 3:1 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were further digested by TAQI (GoTaq® G2 Flexi DNA Polymerase; Promega, cat. no M7805) to assess if the point mutation is or not present ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were subsequently cloned into vectors using pGEM-T Easy Vector System II (Promega, Cat# A1380) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred into 0.2 ml PCR tubes containing 2μl of lysis buffer (0.7% Triton X-100, 2U/μl RNasin (Promega), 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’ ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing rsbU or rsbT/rsbUY28I were subjected to PCR mutagenesis using GoTaq DNA polymerase mix (Promega) supplemented with 2mM additional MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... as well as 1kB DNA ladder were purchased from New England Biolabs while GoTaq PCR reagents (Promega) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed on a CFX96 q–PCR System using SYBR Green qPCR master mix (Promega, USA). GAPDH was used as the internal control ...
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Immunology 2019Quote: ... using a luciferase assay kit (Promega). Results were normalized to β-galactosidase activity (Galactostar ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... with the TNT kit (Promega: L1770). Each substrate was amplified with primers by PCR to allow T7-dependent transcription of the PCR product or transcribed directly if plasmids contained a T7 promoter ...
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bioRxiv - Microbiology 2019Quote: A Luciferase Assay System kit (Promega) was used ...
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bioRxiv - Plant Biology 2019Quote: The Reverse Transcription System Kit (Promega) was used to synthesize a cDNA library following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... or Luciferase Assay System kit (Promega) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... the PureYield™ MidiPrep Kit (ProMega) and NucleoBond Xtra™ Midi/Maxi Kit (Macherey & Nagel ...
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bioRxiv - Genetics 2021Quote: ... using the Renilla Glo Kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the Glutamate Glo Assay Kit (Promega) was used ...
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bioRxiv - Microbiology 2021Quote: ... the Glucose Uptake-Glo kit (Promega) was used ...