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Citations for Promega :
1951 - 2000 of 5335 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The T7 RiboMax express kit (Promega) was used for the in vitro production of 16S rRNA under standard conditions.
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bioRxiv - Molecular Biology 2019Quote: ... using QuantiFluor ONE dsDNA kit (Promega) and library size was checked on the Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2019Quote: ... using a luciferase assay kit (Promega). Results were normalized to β-galactosidase or Renilla activity (Galactostar ...
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bioRxiv - Molecular Biology 2020Quote: ... using the dual luciferase kit (Promega). Experiments were done at least in triplicate ...
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bioRxiv - Biochemistry 2020Quote: The “ADP-Glo” kit (Promega, Wisconsin) was used to measure hydrolysis of ATP to ADP and inorganic phosphate by ATPase ...
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bioRxiv - Bioengineering 2020Quote: ... Steady-Go luciferase assay kit (Promega) was used to measure the expression of luciferase in cells ...
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bioRxiv - Molecular Biology 2021Quote: ... a kit (Promega, Fitchburg, WI, USA) was used to perform TUNEL staining in accordance with manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... using GoTaq qPCR kit (Promega A6001). Plates were sealed using a microseal ‘B’ Adhesive Seal (Bio-Rad MSB1001 ...
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bioRxiv - Biochemistry 2020Quote: ... Using PureYield Plasmid Midiprep kit (Promega), DNA preparations of selected clones were subjected to sequencing at Eurofins Genomics Canada.
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bioRxiv - Cell Biology 2021Quote: ... with a HiBiT assay kit (Promega).
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bioRxiv - Microbiology 2021Quote: ... The Luciferase Assay System kit (Promega) was used to measure the luciferase activity in sporozoite samples following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and NADP+/NADPH-Glo kits (Promega), respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... GoScript Reverse Transcription Kit (Promega, #A5003) was used as per manufacturer instructions with the following modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... using SIRT-Glo Assay kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... as per the kit’s instructions (Promega), 75,000 mouse Fcγ receptor IV (mFcγRIV)-expressing Jurkat effector cells were added to each well to engage with the Fc region of M2e serum antibodies bound to native M2e expressed on the surface of the infected cells ...
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bioRxiv - Bioengineering 2023Quote: The NanoGlo luciferase assay kit (Promega) was used to determine the amount of functional Nanoluc protein in lysed biological samples by supplying the enzyme with substrate and immediately measuring the resulting luminescence ...
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bioRxiv - Cell Biology 2023Quote: ... Dual-Luciferase Assay Kit (E2920, Promega) was used to measure the dual-luciferase activity according to the manufacturer’s instruction.
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bioRxiv - Plant Biology 2023Quote: ... RNA of roots and nodule primordia was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega), as previously described (53) ...
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bioRxiv - Developmental Biology 2023Quote: ... and the Reverse transcription kit (Promega) was used to make cDNA following manufacturer indications ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega) [39] ...
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bioRxiv - Immunology 2024Quote: ... The dual luciferase assay kit (Promega) was used to determine the luciferase activity ...
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bioRxiv - Developmental Biology 2021Quote: ... abdomens were washed with phosphate-buffered saline with 0.1% Tween (PBST) four times for 15 minutes before RNase (Promega, original concentration of 4 mg/mL) was added in a concentration of 10 μL/mL for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Unattached cells were removed by once washing with PBS and adhered cells were incubated with starvation media containing CellTiter-Blue Cell Viability reagent (resazurin; Promega, WI, USA; 36 times dilution). To determine the ratio of attached cells in various conditions ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from blood samples using commercial kit (Wizard® Genomic DNA Purification Kit; Promega), following the fabricant protocol ...
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bioRxiv - Microbiology 2019Quote: Plasmid pGEMt was used for the ligation of purified PCR products according to manufacturer’s protocol (Promega A1360, USA). The reaction mixture includes 5µl of buffer 2X ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR product was gel purified and sub-cloned into the pGEM®-T Easy Vector System (Promega) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated ∼18 hours at 37°C and individual white colonies screened for insert by colony PCR using primers M13_F (5’-ACGACGTTGTAAAACGACGGCCAGT-3’) and M13_R (5’-ATTTCACACAGGAAACAGCTATGACCA-3’) GoTaq DNA Polymerase (Promega). Reactions were separated by gel electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... and transformants were screened in a colony PCR for inserts of the correct size using GoTaq polymerase (Promega) with pCFD6_F and pCFD6_R primers ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR fragments with lengths is above 500 bp to 2 kb were carried out with GoTaq enzyme (Promega) according to standard protocols ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The WGA products were purified using Wizard® SV Gels and PCR Clean-Up System (Promega, WI, USA). The DNA samples were used for library preparation ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification was performed in a 10- μL reaction mixture containing 5 μL of GoTaq Master Mix (Promega), 5 pmol FAM-labeled universal primer (5′ - FAM-gctacggactgacctcggac-3′) ...
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bioRxiv - Bioengineering 2020Quote: The Round 1 eluted pool was PCR amplified for 10 cycles with GoTaq Hot Start Polymerase (Promega, M5001) (1X Colorless GoTaq Flexi Buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and a 982bp fragment of Gad1 (Corresponding to nt 1015-1996 of NM_008077.2) were PCR-cloned into pGEM Easy T vector (Promega).
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bioRxiv - Plant Biology 2020Quote: The qRT-PCRs/qPCR reactions were set up using the GoTaq® qPCR Mastermix (Promega, Madison, Wisconsin, USA) according to the manufacturer’s instructions in a total volume of 15 μl ...
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bioRxiv - Neuroscience 2020Quote: One µl of bisulfite-converted DNA was subjected to PCR using the G2 Green Mix (Promega, Mannheim – Germany) with bisulfite-specific primers at temperatures and concentrations as indicated in Appendix 3 ...
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bioRxiv - Microbiology 2020Quote: ... PCR#2 product was ligated to pGEM-T easy vector or TOPO vector following the manufacturer’s instructions (Promega). After transformation in bacteria and plasmid amplification ...
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bioRxiv - Microbiology 2020Quote: ... The KAN PCR amplicon with compatible ends was ligated into the plasmid backbone using T4 DNA ligase (Promega). The resultant pEXP5/Kan vector was linearized using XbaI and Butters gp31FLAG was ligated into the plasmid for transformation into chemically competent BL21 cells ...
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bioRxiv - Cell Biology 2020Quote: ... mutagenesis primers were used for PCR amplification of pRK7-FLAG-Rheb using pfu Turbo Polymerase (Promega, NSW, Australia) to induce specific mutations ...
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bioRxiv - Cell Biology 2021Quote: A probe corresponding to the final 1078 bp of rps6ka3a (RSK2a; NM_212786.1) was generated by cloning a PCR-derived cDNA fragment into in pGEMT-Easy (Promega), linearising with ApaI (NEB ...
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bioRxiv - Microbiology 2021Quote: ... The pooled amplicons were gel purified using the Wizard SV Gel and PCR Clean-Up System (Promega, USA) and the library was denatured and diluted following manufacturer’s guidelines (Illumina document no ...
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bioRxiv - Molecular Biology 2022Quote: RepEx-PCR-generated ~100x CAG repeats were inserted into a dual-luciferase reporter plasmid modified from pmirGLO (Promega) between firefly luciferase (FLuc ...
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bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
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bioRxiv - Molecular Biology 2022Quote: ... and the amplified DNA fragments were purified using Wizard SV Gel and PCR Clean-Up System (Promega #A9282). DIG-labelled RNA probes were synthesized using T7 or SP6 RNA polymerase and DIG RNA labeling Mix (Roche #11277073910) ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... and amplified PCR products using primers listed in Table S1 were sub-cloned into pGEM-T Easy (Promega). Purified plasmid DNAs were sequenced using M13 primers.
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bioRxiv - Biochemistry 2021Quote: Linear DNA templates were generated by PCR using appropriate primers and transcribed into mRNA with T7 polymerase (Promega). In vitro translation was performed in rabbit reticulocyte lysate (Promega ...
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bioRxiv - Genetics 2022Quote: ... 2 µl of the extracted genome solution was used as a template for Genome PCR by GoTaq (Promega). The sequences of gw182 gene specific primer set designed around the CRISPR target site were 5’ -GAG TCC AAT TTG AGA AAC GGA GGT CA-3’ and 5’ - CTG ATC GTT TGC GCT TAA CTT CAT TAA TTC T-3’ ...
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bioRxiv - Cancer Biology 2022Quote: ... The first step of PCR was carried out with 1 µl of cDNA and DNA polymerase GoTaq (Promega) according to the manufacturer’s instructions and consisted of 95 °C for 5 min and 15 cycles of 95 °C for 30 seconds ...
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bioRxiv - Genomics 2022Quote: ... PCR fragments were either sequenced directly or cloned using the pGEM-T Easy Vector System (Promega, Cat#: A1360) and Sanger sequenced to resolve insertion characteristics ...
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bioRxiv - Biochemistry 2019Quote: ... The N-terminal 312 aa Halo tag sequence was PCR amplified from His6HaloTag® T7 Vector pH6HTN (Promega) as a 5’ SpeI ...
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bioRxiv - Genetics 2019Quote: ... 0.8 µM of both the FAM labelled forward primer and TPP_M13R and 0.2 µM 5R_TPP using GoTaq® Long PCR Polymerase (Promega). A standard 60TD55 protocol was utilized (94°C denaturation for 30 s ...