Labshake search
Citations for Promega :
1751 - 1784 of 1784 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... Dual-luciferase assays were performed using 40 μl of the substrate in the appropriate buffer for a 10 μl in vitro translation assay (Promega, Cat. No. E1960). Luminescence was measured using TECAN Infinite M1000 and plotted on GraphPad (Version 8.4.2).
-
bioRxiv - Neuroscience 2021Quote: ... plates were removed from the incubator after 70 hours and 10 µL of 4x RT-Glo substrate and enzyme per well (Promega, Madison, WI, USA) diluted in P/S free medium ...
-
bioRxiv - Cancer Biology 2021Quote: ... short tandem repeat analysis verified the identity of mouse (Bioassay Methods Group, National Institute of Standards and Technology) and human (Promega GenePrint 10 System) cells.
-
bioRxiv - Cancer Biology 2020Quote: ... the plates were incubated at room temperature for 10 minutes and the luminescence signal was measured with a GloMax® microplate reader (Promega; Madison, WI).
-
bioRxiv - Immunology 2022Quote: ... and the RNA was precipitated with an equivalent volume of isopropanol and incubated at 55°C for 10 minutes before being resuspended in 50 μL of RNase-free water (Promega, Madison, WI, USA). We determined the concentrations of RNA using a Smart-Spec plus spectrophotometer (Bio-Rad) ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691) from Promega (Madison, WI, USA). Approximately 72 hours after transfection ...
-
bioRxiv - Molecular Biology 2021Quote: ... Larvae with the same genotype were pooled (min. n=10 larvae/sample) and RNA was extracted using the ReliaPrep RNA Miniprep System (Promega AG, Dübendorf, Switzerland) following manufacturer’s user guide ...
-
bioRxiv - Microbiology 2021Quote: ... A 2 ml aliquot of the overnight enrichment was centrifuged at 6000 RPM for 10 minutes and then DNA was extracted using the Maxwell RSC Cultured Cells DNA Kit (Promega, Madison, WI, USA). Extraction was performed according to manufacturer’s recommendations with the addition of RNase A after lysis ...
-
bioRxiv - Cell Biology 2021Quote: Isolated protein aggregate fraction was solubilized for 1 h at room temperature with the use of 10 µl 0.1 % ProteaseMAX™ surfactant (Promega, cat. no. V2072) in 50 mM ammonium bicarbonate ...
-
bioRxiv - Cancer Biology 2020Quote: ... service by analyzing DNA samples from each individual cell line for polymorphic short tandem repeat (STR) markers using the GenePrint 10 kit from Promega (Madison, WI, USA).
-
bioRxiv - Immunology 2023Quote: ... 1.5 μL of N2 primers and probe (2019-nCov CDC EUA Kit, Integrated DNA Technologies) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
-
bioRxiv - Microbiology 2022Quote: ... The viral RNAs were extracted from the homogenized lung tissue samples (10% w/v) and nasal wash samples using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI). The whole genomes of SARS-CoV-2 used in this research were amplified using a modified ARTIC protocol with several primers replaced or added (57 ...
-
bioRxiv - Biochemistry 2022Quote: ... the membrane was rinsed 10 min three times in PBS 1X-Tween buffer before ECL (Western Blotting Substrate, # W1001 Promega, Madison, WI, USA) revelation by image acquisition with the Fusion Fx chemiluminescence imaging system (Vilber Lourmat ...
-
bioRxiv - Microbiology 2023Quote: ... Standard calibration curves were performed using a 10-fold serial dilution (103 to 108 molecules) of standard pGEM-T Easy plasmids (Promega, Madison, WI, USA) containing target sequences from Escherichia coli K12 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Authentication of cell lines was confirmed regularly by short tandem repeat (STR) profiling by using the GenePrint 10 kit (Promega, Madison, WI, USA) on an ABI 3730 DNA analyzer (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 μL of 7.5 M guanidine hydrochloride was added to 10 μL of reaction mixture and incorporated radioactivity was captured on SAM2® Biotin Capture Membranes (Promega, Madison, WI) and quantified using liquid scintillation counting as described before.16
-
bioRxiv - Cell Biology 2024Quote: ... RT-qPCR analysis was done on a QuantStudio™ 5 Real-Time PCR System in a final volume of 20 µl containing 10 µl of GoTaq qPCR Master Mix (Promega, Cat No. PRA6001), 2 µl of the forward plus reverse primers (final primer concentration ...
-
Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: HEK293 cells growing at 70% confluency in a 10-cm dish were transfected with wild-type human NOPR or NOPLight (3 μg DNA) and GloSensor-20F (Promega, 2.5 μg DNA) using 12 μL Lipofectamine 2000 (Thermo Fisher ...
-
bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in 200 μL of 0.02 μm filtered ultrapure water and treated with DNase (briefly, incubation at 37°C for 30 min after adding 10 units of RQ1 RNase-free DNase, Promega, Madison, WI, USA). We then extracted DNA using the DNeasy PowerSoil Pro kit (Qiagen ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tryptic digestion was performed by the addition of sequencing-grade modified trypsin (Promega; 25 µL of 10 ng/mL in 50 mM NH4HCO3) and overnight incubation at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... Standard calibration was performed from a 10-fold serial dilution (103 to 108 molecules) of standard pGEM-T Easy plasmids (Promega, Madison, WI, United States) containing target sequences from Escherichia coli K12 ...
-
bioRxiv - Bioengineering 2022Quote: ... was performed to measure the specific killing activity of anti-HIV CAR-T cells toward LHL2/3 cells and LEL6 cells at different ratios from 1:1 to 10:1 by using the CytoTox 96 non-radioactive cytotoxicity kit (Promega, Madison, WI, United States) following the manufacturer’s instructions ...
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... COS-7 cells were plated on 10 cm plates and transfected 24h prior to lysis using FuGENE 6 (Promega; 6-12 µg total DNA). For siRNA tests ...
-
bioRxiv - Cell Biology 2022Quote: The number of viable cells inside HTM hydrogels subjected to the different treatments for 10 days was quantified with the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (Promega; Thermo Fisher Scientific) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... Amplifications were set up in a total volume of 20 μL containing: 10 μL GoTaq® Green Master Mix (Promega Corporation, Madison, Wisconsin, USA), 1 μL of each 10 μM primer ...
-
bioRxiv - Microbiology 2022Quote: ... the gel lane was cut into 10 bands and the proteins were subjected to in-gel digestion with trypsin (Promega, Madison, WI, United States) as previously described (Leijon et al. ...
-
bioRxiv - Cancer Biology 2023Quote: ... All cell lines were authenticated by STR profiling (Generi Biotech, Hradec Králové, Czech Republic; Westmead Institute of Medical Research, Westmead, NSW, Australia; Promega Geneprint 10, Madison, Wisconsin, USA) and routinely tested for mycoplasma by PCR [72].
-
bioRxiv - Cell Biology 2023Quote: ... COS-7 cells were plated on 10 cm plates and transfected 24h prior to lysis using FuGENE 6 (Promega; 6-12 µg total DNA). Cells were routinely tested for mycoplasma using a MycoAlert detection kit (Lonza ...
-
bioRxiv - Immunology 2023Quote: ... stable HEK293 EBNA cell lines were generated using the sleeping beauty transposon system.91 Expression constructs were co-transfected with a transposase plasmid (10:1) into the HEK293 EBNA cells using FuGENE® HD transfection reagent (Promega GmbH, Madison, USA) in DMEM/F12 supplemented with 6% FBS ...
-
bioRxiv - Biochemistry 2024Quote: ... The 5 mL HEK293T cell suspension was combined with the mixture of 10 µL of Nano-Glo® Live Cell Substrate and 90 µL of Nano-Glo® LCS dilution buffer (Promega, Cat.# N2012). The whole mixture of HEK293T with the Nano-Glo® reagents was added to 96 well plates (Greiner Bio-One ...
-
bioRxiv - Biophysics 2023Quote: ... The 12-230 kDa separation module was used for PCNA (CST, D3H8P, Rabbit, 1:100 dilution, #13110), b-Actin (CST, D6A8, Rabbit, 1:50 dilution, #8457S) and Halo (Promega, Mouse, 1:10 dilution, #G9211) detection ...
-
bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900 μL of 0.35 M KCl (10 mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
-
bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
-
bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...