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Citations for Promega :
1651 - 1700 of 1784 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: The HaloTag-fused proteins expressed in neurons (10 DIV) placed on coverslips were fluorescently labeled with 1 µM AF660 (Promega) in medium for 15 min at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... cells were assessed for eGFP expression using Cytation 10 or for NLuc activity using the Nano-Glo luciferase assay (Promega).
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bioRxiv - Molecular Biology 2024Quote: Halo-tagged fusion proteins (200 pmol) were bound to 10 μL of Magne® HaloTag® Beads (20% slurry, Promega), followed by washing ...
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bioRxiv - Molecular Biology 2024Quote: 1-4 μg of total RNA was used for reverse transcription in a 20 μl reaction volume with 10 μM VNN-(dT)20 primer using the GoScript Reverse Transcriptase (Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... with one half treated with DNase (briefly, incubation at 37°C for 30 min after adding 10 units of RQ1 RNase-free DNase, Promega) and one half not treated with DNase (considered non-DNase-treated virome samples) ...
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bioRxiv - Immunology 2024Quote: ... Ten to twenty 10 µm cryosections were used for nucleic acid extraction using the automated Maxwell® RSC instrument (Promega) with the appropriate sample kit and according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Cancer Biology 2024Quote: ... Short tandem repeat-based authentication of cell lines was performed at the Genomics and Transcriptomics Laboratory (GTL, Heinrich Heine University Düsseldorf, Germany) using the GenePrint 10 system (Promega, #B9510).
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bioRxiv - Microbiology 2024Quote: ... 100 µL of the culture was mixed with 10 µL with the NanoLuc Reaction Buffer of the Nano-Glo Luciferase Assay System (Promega). Luciferase activities were measured every 2 minutes with a Tecan Spark microtiter plate ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected tissue was homogenized in lysis buffer (10% Triton X-100, 0.1 M DTT, 40 U/μL RNasin (Promega, N2115), protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: Reverse transcription was performed with 3 µg of total RNA in a 20 µl reaction volume with 10 µM VNN-(dT)20 primer and the GoScript Reverse Transcriptase (Promega). Probe-based multiplex quantitative RT-PCRs were performed with the PrimeTime Gene Expression Master Mix (IDT) ...
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bioRxiv - Genetics 2024Quote: ... including the two strains used for the mapping studies was isolated from 20 females (2 × 10 flies/prep) from each line using the Maxwell 16 Tissue DNA purification kit on the Maxwell 16 instrument (Promega). Samples were eluted in 300 μl water containing RNase A at 20 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... PZ-HPV-7 cells expressing Halo-GPI were washed twice with HBSS and incubated with 10 μg/mL rabbit polyclonal anti-Halo antibody (G928A, Promega) in the same solution for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Ten to twenty 10 µm cryosections were used for nucleic acid extraction using the automated Maxwell® RSC instrument (Promega) and Maxwell RSC simplyRNA tissue kit according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... dye was withdrawn from stained colonies with 10% acetic acid and the associated absorbance was measured using a microplate reader at 600 nm (GloMax Explorer system, Promega)(45).
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bioRxiv - Evolutionary Biology 2024Quote: ... resuspended in 15 mL Ma lysis buffer (50 mM KxHyPO4 pH 7, 10 mM MgCl2) with 20 units of RQ1 DNase (Promega) and a Pierce protease inhibitor mini tablet (ThermoFisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 mM HEPES pH 7.4, 1mM MgCl2 with 1x c0mplete™ EDTA-free Protease Inhibitor Cocktail and 1U/μl RNasin® Plus RNase Inhibitor from Promega added right before the use) and incubating for 30 minutes on ice with pipetting every 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Intracellular NanoBRET assays were performed as previously described (10) using NanoLuc®-STK3 Fusion Vector Cat.# NV4301 (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reaction mixture was prepared for a 10 μL reaction volume using a GoTaq® DNA Polymerase PCR mix (Promega): 2 μL 5X Green GoTaq® Reaction Buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reaction mixture was prepared for a 10 μL reaction volume using a GoTaq® DNA Polymerase PCR mix (Promega): 2 μL 5X Green GoTaq® Reaction Buffer ...
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bioRxiv - Genomics 2020Quote: ... standard curves were generated by preparing 10 fold dilution series of plasmid DNA containing parasite 18SrRNA gene54 and from human genomic DNA (Cat No. G304, Promega, Australia). Thermal cycling was performed on a Light Cycler 480 II (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113).
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bioRxiv - Molecular Biology 2021Quote: ... In vitro translation reactions were carried out with 5 ul of either supplemented rabbit reticulocyte lysate or oocyte extract in total volume of 20 µl containing 10 µM amino acid mix (Promega, L4461), 0.5 units SUPERase•In ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were diluted 10-fold with 100 mM Tris (pH 8.0) and incubated overnight at 37°C with sequencing grade trypsin (Promega, Madison, WI) added at a 1:50 enzyme:substrate ratio (wt/wt) ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular debris was then pelleted by centrifugation and either 5 μl or 10 μl of the clarified supernatant used in dual luciferase assays following the manufacturer’s prescribed protocol (Promega, cat # E1910). Data are shown as the ratio of RLUC to FLUC activity averaged over six experiments.
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bioRxiv - Cancer Biology 2021Quote: ... All cells were routinely tested for mycoplasma and authenticated by short tandem repeat (STR) profiling using the GenePrint 10 System (Promega, B9510).
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bioRxiv - Microbiology 2020Quote: ... the culture media were harvested and Nanoluc activity was determined in 10 µl aliquots of culture medium using a Nano-Glo™ Luciferase Assay System (Promega). To normalize the electroporation efficiency ...
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bioRxiv - Cell Biology 2020Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113). After digestion ...
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bioRxiv - Biophysics 2021Quote: ... U87 and U251-MG cell lines were authenticated by performing short tandem repeat analysis on isolated genomic DNA with the GenePrint® 10 System (Promega), and peaks were analyzed using GeneMarker HID (Softgenetics) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein material was reduced with tris(2-carboxyethyl) phosphine (TCEP; 10 mM final) and digested overnight with 2 μg sequence-grade modified trypsin Gold (Promega, V5280) in 50 mM ammonium bicarbonate (NH4HCO3 ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were validated by STR DNA fingerprinting using the Promega GenePrint® 10 System according to manufacturer’s instructions (Promega #B9510). HT29 ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA from SVF or 3T3-L1 adipocyte-differentiated cells (at day 10 of differentiation) (200 ng) was reverse transcribed using (MMLV) reverse transcriptase following the manufacturer’s protocol (Promega - Madison, WI). Expression of genes encoding the Pparγ2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The yeast colony was grown overnight in 10 ml of YPD and the gDNA was extracted using the Wizard Genomic DNA Isolation kit (Promega, Switzerland). The PCR reaction was as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... The RSB/RNasin buffer (2 ml) was prepared fresh and contained 200 ul of 10 X RSB and 50 ul RNasin (N2511, Promega, USA). The PEB buffer (10 ml ...
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bioRxiv - Biochemistry 2022Quote: ATPase assays for COQ8ANΔ250 were performed essentially as described in Reidenbach et al.10 The reaction was initiated by adding a mixture of ATP (Promega V703), 2-propylphenol (Sigma W352209) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR (qPCR) reactions were conducted in a 10-µL total volume using a 2× GoTaq® qPCR Master Mix (Promega) and run on an ABI 7500 qPCR thermocycler (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Microbiology 2022Quote: ... then diluted 1:5 with nuclease-free water before 2 µL was used as template in a 10 µL GoTaq 1-Step RT-qPCR (Promega, A6020) reaction (69) ...
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bioRxiv - Cell Biology 2022Quote: The number of viable cells inside HTM hydrogels subjected to the different treatments for 10 d was measured with the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (MTS; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the ORF8 plasmid together with the transposase coding plasmid (1/10) with Fugene HD (Promega, Germany) and after one day selected with 3 µg/ml puromycin for three days ...
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bioRxiv - Cancer Biology 2022Quote: ... Sorted cells were collected in 1.5 ml Eppendorf tubes containing 200 µl of DMEM 10% FBS medium and immediately processed for RNA extraction using ReliaPrep™ RNA Cell Miniprep System (Promega) and DNAse TURBO (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Thereafter 10 μl Nano-Glo Live Cell reagent was added according to the manufacturer’s protocol (Promega, Cat No: N2011, Madison, USA), and the cells were incubated in a 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... cell pellets were lysed in polysome lysis buffer (gradient buffer containing 100 mM KCl, 10 mM MgCl2, 0.1% NP-40, 2 mM DTT, and 40 U/ml RNasin; Promega, Leiden, Netherlands), and onto 17–50% sucrose gradients and ultracentrifuged for 2 h at 40,000 rpm ...
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bioRxiv - Genomics 2021Quote: ... 23.5 μl PCR master mix (20 μl Buffer 5X, 2 μl dNTPs 10 μM, 1.5 μl GoTaq; Promega, cat. no. M3001), and 5 μl of Forward universal primer ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μl) were mixed with the master mixes containing 1 μl primers (10 μM each) and reagents of GoTaq G2 (Promega #M7845) and the final volume was 25 μl ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was extracted from the aerial portions of 10-day old seedlings using the Wizard® Genomic DNA Purification Kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The samples were subsequently quenched with 10 mM DTT with overnight digestion at 37°C using Trypsin gold (Promega, WI, USA). The digestion was terminated and the surfactant was cleaved by treating the samples with 200 mM HCl (Sigma ...
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bioRxiv - Immunology 2023Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113). After digestion ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl of in vitro translation (IVT) reaction was performed in varying concentrations of sucrose using 10 μl wheat germ extract (Promega, L4380), 1.6 μl complete amino acid mixture (Promega ...