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Citations for Promega :
1551 - 1600 of 1784 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Dried pieces of gel were rehydrated for 45 min at 4°C in 30 μL of a solution of 50 mM AB containing trypsin at 10 ng/ μL and 0.01% of Protease Max (PM) Surfactant trypsin enhancer (Promega). Subsequently ...
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bioRxiv - Microbiology 2024Quote: RNA was incubated at 95°C for 2 min and on ice for 2 min before 3.3 x RNA folding buffer (100 mM HEPES pH 8.0, 100 mM NaCl and 10 mM MgCl2) and RNasin Plus RNase Inhibitor (Promega) was added to a final volume of 10 μl and incubated for 20 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... which is produced by our DEVA Holding A.S team in Türkiye was incubated with 10 μL IdeS protease (PROMEGA, USA) for 3 hours at 37 °C to cleave the Fc region below hinge region ...
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bioRxiv - Neuroscience 2024Quote: ... Conditioned media was centrifuged at 1000g for 10 min and supernatant was either suspended in Passive Lysis buffer (Promega) (total hTau ...
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bioRxiv - Immunology 2024Quote: ... the RNA/cDNA double-stranded hybrid was then added to 1.5 μl of Blue Buffer (Enzymatics), 1.1 μl of dUTP mix (10 mM dATP, dCTP, dGTP and 20 mM dUTP) (Promega), 0.2 μl of Rnase H (5 U/μl) ...
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bioRxiv - Molecular Biology 2024Quote: ... MikroWin Version 4.41) using a reading time of 10 s upon injection of 50 µl Renilla luciferase reagent (Promega). Three independent experiments (each in technical triplicates ...
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bioRxiv - Neuroscience 2024Quote: ... in which the promoter of period 3 gene drives expression of luciferase 89 were entrained on a 14h light/10 h dark cycle until 5 dpf and then placed in groups of 50 in ⌀10 cm petri dishes in water containing 0.5 mM beetle luciferin (Promega) in 2500 Lux illumination ...
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bioRxiv - Biochemistry 2024Quote: ... The 10 mM stock solution of UDP was taken from the UDP-Glo™ Glycosyltransferase Assay (Promega, Part #V698A).
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... After two low stringency washes for 5 minutes each with 2× saline sodium citrate/ 0.1% sodium dodecyl sulfate buffer (2x SSC buffer; Promega Co., Madison, WI, Catalog# V4261) and a high stringency wash for 2 min with 1x SSC buffer ...
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bioRxiv - Immunology 2021Quote: ... Luminescence was measured after 21 hours of incubation at 37°C with 5% CO2 with a luminometer using the Bio-Glo-TM Luciferase Assay Reagent according to the manufacturer’s instructions (Promega, Cat. Nr.: G7018 and G9995).
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bioRxiv - Cancer Biology 2023Quote: ... Secondary HRP-conjugated antibodies were added at a dilution of 1:10,000 in 5% milk in TBST and incubated for 60 minutes at room temperature (Anti-Mouse: Promega #W402B; Anti-Rabbit: Promega #W401B). The Pierce SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific #34577 ...
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bioRxiv - Neuroscience 2022Quote: ... the pHAD plasmid was linearized by PmeI enzyme to expose the ends of the 5’ and 3’ inverted terminal repeats (ITRs) and transfected into 116 producer cells (Profection® Mammalian Transfection System, Promega, Madison, WI, USA). Helper virus (HV ...
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bioRxiv - Biophysics 2021Quote: ... cells were grown on glass coverslips for 24 h and then transfected with both mCherry-lamin B1-10 and GFP-connexin-43 using ViaFect (Promega). The mCherry-lamin B1-10 was a gift from Michael Davidson (Addgene plasmid #55069) ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 10 μL of isolated RNA with 1 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: Reverse transcription was performed with 1-4 µg of total RNA in a 20 µl reaction volume with 10 µM VNN-(dT)20 primer and the GoScript Reverse Transcriptase (Promega). For the semi-quantitative end-point PCRs the MyTaq Red Mix (Bioline ...
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bioRxiv - Molecular Biology 2021Quote: ... and luciferase activity in 10 μl aliquots of the cell lysates was measured using the Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... Each aliquot was diluted eight-fold with 50 mM ABC and 10 μg of sequencing grade modified trypsin (Promega #V5113) was added ...
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bioRxiv - Microbiology 2022Quote: ... washed three times (10 minutes each) with PBST before incubation for 1 h with α-Mouse IgG HRP Conjugate (Promega) secondary antibody (1:10 000 in PBST) ...
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bioRxiv - Molecular Biology 2020Quote: Preserved tissue (10-15 mg) was used for nucleic acid purification by employing the Wizard genomic DNA kit (Promega, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of this RNA was treated for 10 minutes at 65 °C with a unit of RQ1 RNAse-free DNase (Promega) in a total reaction volume of 10 µl ...
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bioRxiv - Molecular Biology 2020Quote: ... To knock-down cells we mixed 10 μg of dsRNA per million cells with 7.5 μl of Fugene HD (Promega; E2311) in serum-free media and we incubated the dsRNA cocktail with exponentially growing cells for at least 3 days.
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bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
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bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cysteines were then alkylated with 0.5 M iodoacetamide in 100 mM NH4HCO3 (45 minutes in a dark room at room temperature) and proteins were digested overnight with 10 ng/μl trypsin (CAT NO V5280, Promega) in 25 mM NH4HCO3 77 ...
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bioRxiv - Biochemistry 2020Quote: ... Gel pieces were cooled on ice and rehydrated with 100 μ L of 10 ng/μL Trypsin Gold (Promega, UK) for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... before treatment without or with OSM (10 ng/ml, 45 min) and RNA extraction (ReliaPrep RNA Cell Miniprep System, Promega). cDNA was generated using oligo(dT)20 primer (GoScript Reverse Transcription System ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated at room temperature for 10 minutes to stabilize luminescent signal before luminescence was recorded on a GloMax plate reader (Promega).
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted 10-fold in 50 mM Tris-HCl and digested with Sequencing Grade Modified Trypsin (Promega - V5111) at a Protein:Trypsin ratio 50:1 overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... at room temperature, with 40 μL assay buffer (CO2-independent medium, 10% FBS) supplemented with 2% (v/v) GlosensorTM reagent (Promega). To stimulate endogenous cAMP via β adrenergic-Gs activation ...
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bioRxiv - Microbiology 2021Quote: ... mice were anesthetized with ketamine-xylazine (100 mg/kg ketamine and 10 mg/kg xylazine) and then retro-orbitally injected with 100 µl of Nano-Glo luciferase substrate (Promega) diluted 1:10 in PBS ...
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bioRxiv - Plant Biology 2020Quote: ... The concentrations of the resulting cDNAs were adjusted to 10 ng/µL and 2 µL were used for RT-qPCR reactions performed with the GoTaq qPCR Master Mix (Promega) in a final volume of 10 µL ...
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bioRxiv - Microbiology 2020Quote: ... at 9 h post-electroporation and luciferase activity was measured in 10 µl aliquots of the cell lysates using a Luciferase Reporter Assay System (Promega).
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bioRxiv - Cell Biology 2020Quote: ... gel pieces were reswelled in 60 μL of 50 mM NH4HCO3 buffer containing 10 ng/μL of trypsin (modified porcine trypsin sequence grade, Promega) incubated for one hour at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... 70 μL of PBS were added to each well followed by addition of 10 μL of a 50 μM coelenterazine-h solution (Promega). After 10-min incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed for 15 minutes on ice then treated with 10 µL of RQ1 DNase (Promega, catalog no. M610A) for 5 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lines were validated by short tandem repeat (STR) DNA typing using the Promega GenePrint 10 System according to the manufacturer’s instructions (Promega #B9510). Cells were cryopreserved in Recovery Cell Culture Freezing Medium (Gibco #12648-010) ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl of the lysate was used to measure luciferase activity by sequential mixing with 10 μl of each substrate from the Dual Luciferase Reporter Assay System (Promega). Luminiscence was measured on a Mithras LB 940 microplate reader (Berthold Technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... The gels were dehydrated with acetonitrile and rehydrated with 10 mM ammonium bicarbonate containing 13 ng µl-1 trypsin (Promega). The protease digestion was carried out for 12 h at 37 °C following which the peptides were extracted from the gels using 1:2 (v/v ...
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bioRxiv - Microbiology 2022Quote: ... and the Gaussia princeps luciferase enzymatic activity was measured on a Centro XS LB960 microplate luminometer (Berthold Technologies, reading time 10 s after injection of 50 µl Renilla luciferase reagent (Promega). For the minigenome assays ...
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bioRxiv - Neuroscience 2020Quote: ... the culture medium was replaced with a CO2-independent medium containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cGMP stock solution (Promega). The cells were then incubated for 2 h at 27°C in the dark ...
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bioRxiv - Plant Biology 2021Quote: ... a 10 mm × 10 mm piece of tissue surrounding the inoculation point was applied to the Dual-Luciferase Reporter Assay System (Promega), to detect the firefly luciferase activity (or activation of the ACS2 and ACS2mut promoters) ...
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bioRxiv - Microbiology 2021Quote: ... air-dried and treated by 10 mkl of 12 mg/mL solution of trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega) in 50 mM ammonium bicarbonate for 15 h at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... The proteins were then incubated for 30 min on ice in the presence of 10 μL of 20 ng/μL trypsin (Trypsin V5280, Promega), followed by 16 h at 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Residual genomic DNA was then removed from each sample with 10 U of RNAse-free DNaseI (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cells were mock treated or treated with 10 unit/mL of uIFN for 6 hrs and collected in 1× passive lysis buffer (Promega). Lysates were assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Five ml of BHI broth containing 10 μg/ml tetracycline was inoculated with a single colony and genomic DNA was extracted (Wizard DNA extraction kit, Promega). Genomic DNA was sequenced by paired-end joining Illumina (Biomics Platform of the Institut Pasteur ...
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bioRxiv - Cancer Biology 2021Quote: ... The reaction mixture was then transformed at a ratio of 1:10 (reaction volume/volume competent cells) into HB101 competent cells (Promega). Spectinomycin-resistant colonies were screened by restriction digestion of miniprep DNA and subsequently validated by DNA sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
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bioRxiv - Plant Biology 2022Quote: ... 100 ng total DNA was added to a 20 μl PCR containing 10 μl GoTaq® Master Mixes (Promega, USA) and 0.1 μM of each primer pair ...