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Citations for Promega :
1701 - 1750 of 1784 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Cell viability was determined at DIV 10 using the CellTiter-Blue Cell Viability Assay according to the manufacturer’s instructions (ref: 8080, Promega, Madison, WI). In brief ...
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bioRxiv - Molecular Biology 2022Quote: ... A-tailed gDNA fragments and 10 μl 30 μM annealed adapters were ligated in a 50 μl ligation reaction using T4 DNA Ligase (Promega, M1804) incubated at room temperature for 3 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluate volume was increased to 10 mL with 50 mM ammonium bicarbonate and treated with sequencing grade trypsin (Promega, V5111) at 1:50 trypsin:protein ratio overnight at 37°C with gentle agitation ...
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bioRxiv - Immunology 2024Quote: ... A total of 10 ng of cDNA was used for quantitative PCR in a total volume of 10 µl with GoTaq® qPCR Master Mix (Promega) and specific primers ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction mixture was then transformed at a ratio of 1:10 (reaction volume/volume competent cells) into HB101 competent cells (Promega #L2011). Spectinomycin-resistant colonies were screened by restriction digestion of miniprep DNA and subsequently validated by gel electrophoresis in a 0.8% agarose gel and by DNA sequencing ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and a final extension at 72 °C for 10 min using GoTaq® G2 Hot Start Green Master Mix (Promega, USA). The PCR products of each gene were then separated on 2% agarose gels containing SYBR® Safe DNA Gel Stain (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were dried with 200 μl of ACN and digested with 10 ng/μl Lys-C (sequencing grade, Promega, USA) in 50 mM NH4HCO3 overnight at 37 °C (14).
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bioRxiv - Cancer Biology 2022Quote: ... to 10.5mL of 2.0 x 10^5 of Hek293 Cells (ATCC CRL1573) in growth media (DMEM + 10% FBS) was added 525uL of a 9:1 ratio of carrier DNA (Promega E4881) to a CLK nLuc construct-CLK1 CLK2 or CLK4 (Promega – NV1131 ...
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bioRxiv - Cell Biology 2022Quote: ... Thereafter 10 μl Nano-Glo Live Cell reagent was added according to the manufacturer’s protocol (Promega, Cat No: N2011, Madison, USA), and the cells were incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 μM was incubated with 100 μl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were confirmed to be free of mycoplasma (PCR-based method) and authenticated by Short Tandem Repeat (STR) analysis using the GenePrint® 10 System (Promega, Madison ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from 10 ⨯ 10 µm FFPE tissue sections using the Maxwell RSC FFPE RNA kit in accordance with the manufacturer’s manual (Promega, United States). RNA concentration was determined using a Qubit 3.0 Fluorometer using the Qubit RNA BR Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed in 1 x PBS followed by lysis in 300 μl of cell lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% NP-40, 1×EDTA-free Proteinase Inhibitor, and 1000 U RNase I, Promega, Madison, WI, USA). The crude nuclei were pelleted and resuspended in 400 μl nuclei lysis buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from 10 whole fly bodies collected on dry ice between ZT 6 and 8 using SV RNA Total Isolation kit (Promega #Z3101) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cell viability was assessed by incubating at 37°C up to 107 cells resuspended in complete RPMI medium containing 10 µM of the CaspACE™ FITC-VAD-FMK in Situ Marker (Promega). After 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 2 μL of cDNA was added to the RT-PCR reaction mixture along with 10 μL of 2x GreenTaq polymerase (Promega, Madison), 1 μL of each MpFV-Hel forward and reverse primers (10 μM) ...
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bioRxiv - Immunology 2024Quote: ... Samples were further diluted to 0.5 M GuHCl and an additional 20 ng/µL LysC was added along with 10 ng/µL Trypsin (Sequencing grade modified, Promega #V5111) and incubated at 37°C for 16 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... Measurements were taken every 10 min in Nunc™ F96 MicroWell™ 446 plates using a GloMax® navigator luminometer (Promega) with 5s integration time and 2s delay ...
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bioRxiv - Biochemistry 2024Quote: ... cells were lysed with 100 µL of 1X luciferase cell culture lysis reagent (25 mM Tris-phosphate pH 7.8, 2 mM DTT, 2 mM 1,2- diaminocyclohexane-N,N,Ń,Ń-tetraacetic acid, 10% glycerol, 1% Triton X-100, Promega), and luminescence was measured using the Luciferase Assay System (Promega) ...
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bioRxiv - Genetics 2024Quote: ... The excised SV40 poly (A) signal fragment was amplified (Primers 9-10 Table S7) and cloned in the pGEM T easy system (Promega A1360).
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bioRxiv - Cancer Biology 2023Quote: ... Each organ was homogenized with a lysis solution containing a 1:5 dilution of Luciferase Cell Culture Lysis 5X Reagent (Promega, Madison, WI, USA, cat. no. E1531) in Millipore water supplemented with 1 % (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... or PACAP (10 nM final) and assayed 3 hours later with the Nano-Glo® Dual-Luciferase® Reporter System (Cat# N1630; Promega) on a Berthold Sirius luminometer to quantify ERK activation.
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bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... specificity of DNA binding was confirmed by observing significant decreases in immunoreactivity in DNA-coated wells that were treated with DNase1 (10 units; RQ1 Dnase, Promega Madison, WI), and in samples of alligator plasma pretreated with 100 μg of calf thymus DNA ...
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bioRxiv - Genomics 2020Quote: ... Total DNA of 500ng of ODInCas9 plasmid together with ZFN-AAVS1 plasmid at a ratio of 1:10 was mixed and transfected by lipid-based transfection using FuGene HD (Promega, Madison, WI) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Luminescence was read after 2 minutes shaking on an orbital shaker and 10 min incubation at RT using the GloMax instrument (Promega, Madison, USA).
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bioRxiv - Cancer Biology 2021Quote: ... with target sequence inserts were cotransfected into HEK293T and MCF7 cell lines with 200 ng of Control/YAP1-S127A construct and 10 ng of Renilla luciferase pRL-TK plasmid (Promega, Cat. #E2241) using Lipofectamine®2000 transfection reagent (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... the Wizard Genomic DNA Purification Kit was used including both lysozyme and lysostaphin (10 mg/ml each) (Ref. A1120, Promega Corporation, Spain); PacBio sequencing ...
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bioRxiv - Microbiology 2021Quote: Protein samples were alkylated with 10 mM iodoacetamide in the dark at 37°C for 30m and subsequently digested with trypsin (Promega, 50 ng) overnight at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... All human cell lines used were authenticated at the Johns Hopkins Genetic Resources Core Facility via short tandem repeat profiling of 9 genomic loci with the GenePrint 10 System (Promega, Madison, WI) before use ...
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bioRxiv - Molecular Biology 2023Quote: ... HAP1 and HAP1-dEGFPY66S cells were imaged in imaging medium (FluoroBrite™ DMEM, 10% FBS, 2.5 mM CaCl2) supplemented with 2 µL CellTox™ Green (Promega, G8741) and 5 µL Annexin V-AV647 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: Ten microliters of supernatant from all the above reactions were incubated with 10 µl of freshly prepared UDP nucleotide detection reagent (Promega, Cat#: V6961) according to the manufacturer’s specifications ...
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bioRxiv - Systems Biology 2024Quote: ... Cell lines were routinely tested for mycoplasma and were periodically authenticated to its early passage using the GenePrint 10 System (Promega, catalog # B9510). VEM and DAS (Selleck Chemicals ...
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bioRxiv - Genetics 2024Quote: ... The qRT-PCR reactions were conducted in a 10 μL volume using the GoTaq qPCR Master Mix chemistry (Promega, Madison, Wisconsin, USA) and a CFX384 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... NanoLuc assays were performed with a 10 μl aliquot of fractions transferred to opaque round bottom 96 well plates and luciferase activity was measured using 10 μl NanoGlo Luciferase Asssay reagent (Promega, Madison, WI) and luminescence was measured on a FlexStation 3 microplate reader (Molecular Devices ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were placed in homogenizer tubes containing ceramic beads along with lysis buffer from the Total RNA Purification Kit (Norgen, 37500) and RNAse inhibitors (10 μl/mL β-mercaptoethanol and 200 units/mL RNAsin Plus (Promega, N2615)) ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and RNA were extracted from a single 10 μm curl using the Maxwell HT FFPE DNA Isolation System (Promega, Madison, WI) on the KingFisher™ Flex instrument (Thermofisher ...
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bioRxiv - Biophysics 2023Quote: ... They were then diluted 5-fold with aqueous 50 mM TEAB and incubated overnight with Sequencing Grade Modified Trypsin (1 ug in 10 uL of 50 mM TEAB; Promega, Madison, WI). Following this an equal volume of ethyl acetate/trifluoroacetic acid (TFA ...
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bioRxiv - Plant Biology 2023Quote: ... The luminescence activity was then recorded with the integration time of 10 seconds in the GloMax Explorer System (Promega Corp., Madison, WI).
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bioRxiv - Biophysics 2024Quote: ... The samples were dried again and rehydrated with 20 µl of trypsin solution with 0.01% ProteaseMAXTM surfactant comprised of 10 ng µl-1 Trypsin (Promega Corp., Madison, WI) in 25 mM NH3HCO3 / 0.01% w/v of ProteaseMAXTM from Promega Corp ...
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bioRxiv - Molecular Biology 2024Quote: ... Authentication of cell lines was conducted using short tandem repeat profiling at the Johns Hopkins University Genetic Resources Core Facility using GenePrint 10 (Promega, cat # B9510).
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bioRxiv - Molecular Biology 2024Quote: ... The crude nuclei were pelleted and resuspended in 400 μl nuclei lysis buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS, 1× EDTA-free Proteinase Inhibitor, and 1000 U RNase I Promega, Madison, WI, USA). Supernatant obtained after sonication and centrifugation steps were digested with DNase I for 20 min at 37°C ...
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Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulencebioRxiv - Microbiology 2024Quote: ... They were then diluted 5-fold with aqueous 50 mM TEAB and incubated overnight with Sequencing Grade Modified Trypsin (1 µg in 10 µl of 50 mM TEAB; Promega, Madison, WI) following which an equal volume of ethyl acetate/trifluoroacetic acid (TFA ...
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bioRxiv - Systems Biology 2024Quote: ... plates were incubated for 10 days in 12L:12D conditions and treated with 50 µl of 1:50 of furimazine substrate solution (Promega, Southampton UK): 0.01% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... five µg protein were reduced by 2.5 mM dithiothreitol for 30 min at 37°C and alkylated with 10 mM iodoacetic acid for 30 min at 37°C in the dark before digestion by trypsin (Promega, Walldorf, Germany) in a protein to enzyme ratio 25:1 overnight at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: Capture plates (384 wells plates) containing a lysis mix (nuclease-free water, 10% Triton X-100, RNasin plus 40U/mL (Promega, Cat#: N2611) and MARS-seq barcodes (Keren-Shaul et al. ...
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bioRxiv - Microbiology 2020Quote: ... for 3 hours before being diluted with 5 volumes of 40 mM NH4HCO3 and digested overnight with sequencing grade modified trypsin (Promega, Madison, WI, USA; 1/50 w/w). Digested samples were acidified to a final concentration of 0.5% formic acid and desalted with 50 mg tC18 Sep-Pak columns (Waters corporation ...
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bioRxiv - Microbiology 2020Quote: ... All reaction plates included minimum triplicate no-template controls and a 10-fold dilution series of pGEM-t-easy (Promega, Madison, WI, USA) cloned standards for the relevant amplicon ...
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bioRxiv - Biophysics 2021Quote: ... Approximately 3 μg of DNase I-treated RNA was reverse transcribed to cDNA using 10 μM of oligo (dT) primer and M-MLV reverse transcriptase (Promega, Madison, WI USA) in a 20 μL reaction ...