Labshake search
Citations for Promega :
1251 - 1300 of 1784 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The final 50 µL translation reaction included the 5 µL RNA mixture described above and components from the Rabbit Reticulocyte Lysate system (Promega): 35 µL of rabbit reticulocyte lysate ...
-
bioRxiv - Biochemistry 2023Quote: ... The first-strand cDNA was synthesized from 5 µg of the total RNA with an oligo (dT) primer using the AMV reverse transcriptase (Promega). A polymerase chain reaction (PCR ...
-
bioRxiv - Cancer Biology 2024Quote: ... Each well was transfected with 500 ng pGL4.22 vector with or without H1-0 promoter expression as well as 5 ng Renilla luciferase control plasmid pGL4.73 (Promega, #E6911), and 250 ng of the respective pcDNA3.1 vectors (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... An amount corresponding to 5 % of the pull-down volume was saved for Western blot analyses and 1 µg of trypsin gold (Promega) was then added to the beads for 16 h at room temperature in a thermomixer at 600 RPM ...
-
bioRxiv - Cell Biology 2024Quote: 2-3 days before the recording the cultures were transferred into a recording medium containing 5% FBS and 0.1 mM luciferin (Promega E1601) (Feeney et al. ...
-
bioRxiv - Biophysics 2024Quote: ... Proteins were digested to peptides by adding 25 µL of 50 mM ammonium bicarbonate containing 5 µg of trypsin (Promega) and incubating at 37 °C for 18 hours ...
-
bioRxiv - Biochemistry 2024Quote: ... elution buffer I (2 M Urea, 50 mM Tris pH 7.5, 1 mM DTT and 5 ng/µl Trypsin (Promega, #V5111)) were added and samples were incubated for 1 h at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... Oligonucleotides encoding guide RNAs targeting M18BP1 (5’- TTGTACTGAAAAAATCATCA-3’) were cloned into pX459-v2 and co-transfected using FuGENE 6 (Promega) with pUC19 containing a 1528 base pair stretch containing the mutated sequence of the locus of interest and homology arms ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were then incubated for 72 h at 37°C and 5% CO2 and cell viability determined by CellTiter-Glo Luminescent Cell Viability Assay (Promega). In short ...
-
bioRxiv - Developmental Biology 2024Quote: ... a minimal promoter (5′-AGACACTAGAGGGTATATAATGGAAGCTCGACTTCCAG-3′) and 8x Gli1-binding sites (110) were cloned into the pGL3-Basic vector (Promega), in which Firefly luciferase was replaced with NanoLuc luciferase amplified from the pNLF-N [CMV/Hygro] vector (Promega ...
-
bioRxiv - Biochemistry 2024Quote: ... then resuspended in 1 gel volume of 25 mM ABC containing 5 ng/µL of digestion grade trypsin (Promega, V5111) and incubated at 37°C for 16 hours for a full digestion or for 15 minutes for a partial digestion ...
-
bioRxiv - Molecular Biology 2024Quote: ... and the chromatin-associated fraction was sonicated on a Bioruptor (5 times 20 sec on/off intervals) prior to DNase treatment using RQ1 DNase (Promega) and RNA extraction using Trizol ...
-
bioRxiv - Microbiology 2024Quote: ... Purified DENV2 EDIII was biotinylated by incubating 100 µg of DENV2 EDIII with 5 nmol of the HaloTag PEG biotin ligand (Promega) at room temperature (RT ...
-
bioRxiv - Microbiology 2024Quote: ... The 25 μl reaction contained 5 μl of PCR Green Buffer with magnesium chloride (Promega, Cat N° M7845, Madison, USA), 1 μl of 5 mM dNTP ...
-
bioRxiv - Microbiology 2024Quote: ... After three rounds of washing in PBST the following Horseradish peroxidase-conjugated secondary antibodies were diluted in 5% milk-PBST and used with at a 1:10000 dilution anti-rabbit (W4018, Promega), 1:5000 anti-mouse (W402B ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells in each well were resuspended in 30 μL of TLR7-Fc or other Fc-tagged proteins (final concentration 5 μg/mL) in 0.5% BSA in 1x DPBS containing 200U/mL RNasein (Promega, N2511), and incubated on ice for 45 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... Exponentially growing and stationary phase cells yielding a total OD600 of 800 were resuspended in 5 ml 1x FastBreak cell lysis buffer (Promega) for 30 minutes on ice and then sonicated afterwards ...
-
bioRxiv - Neuroscience 2024Quote: Ensembl predicted regulatory region including the POU domain binding site on Aspm promoter/enhance (sequence from 5’-GAAAAAGTGGGCAGTAACTCGC-3’ to 5’-CAACCTTTCCCTGAGGACGATC-3’) was synthesized by Twist Bioscience and cloned into pGL3-Basic Luciferase Reporter vector (Promega) using the Gibson assembly method ...
-
bioRxiv - Cell Biology 2024Quote: ... Tris 50mM pH8.0 and samples were heated 5 min at 90°C before digestion in a mix of 500 ng of LysC (Promega) at 37°C for 2h ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNAs were amplified with 6 PCR cycles using short Solexa P3/5 primers to allow beads-based size selection with ProNex Beads Size-selection Chemistry (Promega). In order to avoid over-amplification ...
-
bioRxiv - Biochemistry 2024Quote: ... and wild-type or mutant βarr1 with a C-terminal HA tag with a 1:5 DNA:FuGENE®6 (Promega) ratio according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... samples were centrifuged at 200× g for 5 minutes and then analyzed with the Nano-Glo HiBiT Lytic Detection System (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... a separate set of duplicate cell suspensions were measured at 475 nm excitation and 535 nm emission wavelengths after addition of 5 μM coelenterazine-h (Promega). PBS or 10 μM quinpirole was added to cell suspensions and BRET emissions were immediately measured again ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... The Renilla luciferase activity associated with WT or uORF-mutated 5’UTRs was measured according to the manual of the Dual-Luciferase Reporter Assay System (Promega) 32 h after transfection and was normalized to the activity of firefly luciferase.
-
bioRxiv - Cell Biology 2020Quote: ... The BrdU/C labelled DNA strand was digested with 10 U/ml Exonuclease III (Promega M1811) for two rounds of 10 min at RT ...
-
bioRxiv - Immunology 2021Quote: ... Cells were incubated for 10 minutes at room temperature with 0.35 µg of pRL-TK (Promega) and either 10 µg of each luciferase reporter vector or 5 µg of each reporter and 5 µg of an additional expression vector ...
-
bioRxiv - Molecular Biology 2021Quote: ... the reaction products were split into 10 μL aliquots and S1 nuclease 10x reaction buffer (Promega) was added to 1x concentration ...
-
bioRxiv - Developmental Biology 2021Quote: ... 10 µL of a 0.1 mg/mL stock solution of trypsin (Promega Trypsin Gold MS grade) in 5 mM acetic acid was freshly diluted into a 140 µL solution of 10 mM NH4HCO3 to make the working digestion solution ...
-
bioRxiv - Microbiology 2021Quote: ... A 1:10 solution of sample homogenate to colentrazine was analyzed on a GloMax Explorer (Promega).
-
bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured in DMEM+10% FBS+Penicillin/Streptomycin and transfected using FuGENE 6 (Promega). Co-recruitment assays were performed 24 hours after transfection ...
-
bioRxiv - Microbiology 2020Quote: ... 85 ul of 10% SDS solution and 40 ul of Proteinase K (15mg/ml, MC500B Promega) were added and samples were incubated for 30 minutes at 60° C ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 0 or 1 μg Sequencing Grade Modified Trypsin (0 or 10 μl; #V5111, Promega, WI, USA) that was reconstituted within trypsin re-suspension buffer (50 mM acetic acid ...
-
bioRxiv - Pathology 2020Quote: 10 µL GoTaq® Probe qPCR Master Mix with dUTP (2X) (Promega, Cat # A6120 and A6121)
-
Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Beads were then resuspended in 50 µL trypsinization solution consisting of 10 ng/µL Trypsin (Promega) in 100 mM tetramethylammonium bicarbonate (TEAB) ...
-
bioRxiv - Cell Biology 2021Quote: HEK293E cells were plated and cultured in DMEM with 10% FBS and transfected with FuGENE6 (Promega) 24 hours later ...
-
bioRxiv - Immunology 2022Quote: ... Proteins were digested into peptides with 12μL of 1:10 Trypsin Gold (Promega, Madison, Wisconsin, V528A) in 50mM TEAB per sample ...
-
bioRxiv - Microbiology 2021Quote: ... Vero cells were incubated with 10 μM of the MEK-specific inhibitor U0126 (DMSO soluble, Promega).
-
bioRxiv - Genetics 2020Quote: ... Using a CAPP® 8-Channel Pipette (Starlab) 10 μl of 100 mM VivoGlo Luciferin (Promega) was added to the negatives and then to the mRNA soaked worms ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 10 μl of each supernatant was added to 50 μl of luciferase assay substrate (Promega). We used a Synergy Neo HTS multimode microplate reader (BioTek ...
-
bioRxiv - Molecular Biology 2021Quote: ... The adapter ligated aRNA was then mixed with 1 µl of dNTPs (10 mM each) (Promega) and 2 µl of RTP oligonucleotide (20 µM ...
-
bioRxiv - Genetics 2020Quote: ... PIK3C2B intron 10 fragment was cloned into NanoLuc luciferase reporter construct (pNL3.2[NlucP/minP]) (Promega #N1041) to generate PIK3C2B-pNL construct while NFIA ORF was cloned into pFLAG-CMV expression plasmid to generate NFIA-FLAG construct ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 μl of the reaction mixture was incubated with 30 μl of BactiterGlo (Promega, Madison, WI). Luminescence was determined using a Flex Station 3 plate reader (Molecular Devices ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were transfected with 100 ng pGL3-RARE-luciferase and 10 ng pRL Renilla (Promega E2261) using Mirus TransIT-2020 Transfection Reagent (Mirus MIR 5404 ...
-
bioRxiv - Immunology 2020Quote: ... containing 10 μl/well of RNA catch buffer (10mM Tris pH7.4 supplemented with RNasin (Promega, UK)) ...
-
bioRxiv - Biophysics 2022Quote: ... mixed 1 to 1 (v / v) with 10 μg / ml trypsin (Sequencing Grade Modified Trypsin, Promega) in 50 mM ammonium bicarbonate and incubated for 2 hours at 60 °C for enzymatic hydrolysis ...
-
bioRxiv - Cancer Biology 2022Quote: ... 20 µL of PBS containing 10 μg/mL Hoechst and 1/4000 CellTox Green Dye (Promega) were dispensed per well 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
-
bioRxiv - Neuroscience 2022Quote: ... media was replaced with CO2-independent media + 10% FBS and the GloSensor cAMP reagent (Promega: E1291) at 2% final concentration ...
-
bioRxiv - Immunology 2022Quote: ... 0.5 μl 10 μM Reverse primer and 0.125 U/μl GoTaq DNA Polymerase (Promega, Madison, WI) were mixed to make a 25 μl PCR reaction mix (key resource table) ...
-
bioRxiv - Cell Biology 2024Quote: ... Half of the sample was digested with 10 µg trypsin (Promega, 18 h at 37 °C). DOC was precipitated with 10 µL concentrated formic acid (conc ...
-
bioRxiv - Cell Biology 2023Quote: ... proteinase K digestions were stopped by 10 mM phenylmethanesulfonyl fluoride (final concentration) (Protease Inhibitor Cocktail, Promega) on ice for 20 min ...