Labshake search
Citations for Promega :
1501 - 1550 of 1784 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... benthamiana leaf discs were ground to a fine powder in liquid nitrogen in 2 ml Eppendorf Safe-Lock tube (1 leaf disc in each tube) and subsequently incubated 5 min at room temperature (RT) with 200 µl of the Passive lysis buffer (Promega catalog number E1910). The resulting crude leaf extract was centrifuged at 20,000 g for 2 min at RT ...
-
bioRxiv - Microbiology 2023Quote: ... Three μL (6 ng on average) of template DNA was added to 5 μL 5x GoTaq Green Reaction Mix Buffer (Promega, Madison, Wisconsin, USA), 0.625 units of GoTaq DNA Polymerase (Promega ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 µl of each translation reaction was mixed with 5 µl 1x Renilla-Glo substrate in Renilla-Glo assay buffer and supplemented with 40 µl of H2O (Promega, Cat. No. E2720) on a white-bottom 96-well plate (Greiner ...
-
bioRxiv - Cell Biology 2020Quote: ... Trypsin digestion was performed by addition of 30 µL of 10 ng/µL sequencing grade trypsin (Promega, Madison, WI) in AmBic followed by 30 µL of additional AmBic ...
-
bioRxiv - Molecular Biology 2021Quote: ... were added to the 5′ end labelled 187cspA RNA in a total volume of 10 µL in Buffer O containing 0.4 U/µL of RNasin (Promega). Complex formation was performed at 15°C or 37°C for 15 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... the samples were incubated at either 15°C or 37°C for 10 min in Buffer I containing 0.4 U/µL of RNasin (Promega). Following renaturation ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μL of stopped reactions were added to 50 µL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a 96-well white microplate ...
-
bioRxiv - Molecular Biology 2021Quote: ... 11 μL of substrate mix (10 μL of Promega Nano-Glo® Luciferase Assay Buffer, 0.1 μL of Promega NanoLuc® luciferase substrate and 1 μL of 10 mg/mL CHX ...
-
bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
-
bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
-
bioRxiv - Microbiology 2021Quote: IL-18 truncations were generated through in vitro transcription/translation using pET-pro-IL-18 as a template in 10 µl reaction volumes following the manufacturer’s recommendations (TNT Coupled Reticulocyte Lysate; Promega) as previously [40] ...
-
bioRxiv - Neuroscience 2020Quote: cDNA synthesis was performed in a reaction volume of 25 μl (10 cell pools) or 20 μl (single cells) containing dNTPs (0.5 mM, Promega), random primers (100 ng/per tube ...
-
bioRxiv - Cell Biology 2021Quote: ... Each PCR reaction was performed in a 10 μL mixture containing 0.25 U GoTaq G2 Hot Start Polymerase (Promega), 1x GoTaq G2 Buffer ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 10 μL of stopped reactions were added to 50 µL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a 96-well white flat bottom microplate ...
-
bioRxiv - Systems Biology 2020Quote: ... Samples were further diluted to 0.5 M GuHCl with 10 mM EPPS pH 8.0 and digested with an additional 10 ng/μL LysC and 20 ng/μL sequencing grade Trypsin (Promega) at 37 °C for 16 hours.
-
bioRxiv - Microbiology 2020Quote: ... In vitro transcription/translation was performed with the corresponding primers in Table 1 using pET-pro-IL-1β as a template and following the manufacturer’s recommendations in 10 µl reaction volumes (TNT Coupled Reticulocyte Lysate; Promega). Loading for IL-1R reporter assays was normalized by total IL-1β product measured by enzyme-linked immunosorbent assay (R&D Systems).
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were further diluted to 0.5 M GuaCl with 10 M EPPS pH 8.5 and digested with an additional 10 ng/µL LysC and 20 ng/µL sequencing grade Trypsin (Promega) at 37°C for 16 h ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were digested by adding 100 µL trypsin solution (10 mM TEAB, pH 7.5, 2 µg Promega trypsin #V5111) and incubated overnight at 37 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) (HyClone) with 10% fetal bovine serum FBS (HyClone) and 1% penicillin-streptomycin (P.S.) (Promega) at 37 °C in a 5% CO2 atmosphere.
-
bioRxiv - Microbiology 2020Quote: ... The proteins in the sample were digested with 10 μg/mL sequence grade modified trypsin (Promega, Madison, WI, USA) at 37°C overnight ...
-
Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium (Ham’s F12 with 10% FBS, Penicillin/Streptomycin, 50 mM HEPES) with 1:50 Endurazine luciferase substrate (Promega) added ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were treated with the indicated drugs in combination with pan-caspase inhibitor Z-VAD-FMK (10 mM, Promega). After 24 hours ...
-
bioRxiv - Genomics 2022Quote: ... 2 µl of yeast lysate was used as a template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
-
bioRxiv - Synthetic Biology 2020Quote: ... at 54°C for 10 min and the resulting cDNA amplified with GoTaq Flexi DNA polymerase (Promega; Madison, WI) for 8 PCR cycles in the same reaction with a final volume of 100 μL ...
-
bioRxiv - Plant Biology 2021Quote: ... Purified nucleus were recovered in 300 μl of Nuclei Lysis Buffer (50 mM Tris-HCl pH8; 0.1% SDS; 10 mM EDTA; 100 μM PMSF; RNasin PROMEGA) and 5 cycles (30 s ON – 30 s OFF ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples were diluted 10-fold with 50 mM HEPES pH 8 and incubated with sequence-grade modified Trypsin (Promega) at 1/50 trypsin/protein ratio for 16 h at 37 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... STR profiling was carried out by PCR amplification of nine STR loci plus amelogenin (GenePrint 10 System; Promega, #B9510), fragment analysis (3730XL DNA Analyzer ...
-
bioRxiv - Pathology 2021Quote: ... Vero cells were kept in culture in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% of foetal bovine serum (FBS) and transfected using FuGENE HD (Promega) transfectant reagent as suggested by the manufacturer either with a plasmid encoding the Green Fluorescent Protein (GFP ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were lysed by adding 3x lysis buffer (10 mM Tris-HCl, 1.5% Igepal, 150 mM NaCl, 1 U/µl RNasin (Promega)) ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were digested initially with LysC (WAKO) at 37°C for 2.5 h and subsequently with 10 μg trypsin (modified sequencing grade, Promega) overnight ...
-
bioRxiv - Biochemistry 2020Quote: ... Reactions containing 2.5 pmol protein and 3 pmol 32P-labelled RNA were carried out in a volume of 10 µL with 0.5 U/μl RNasin (Promega), 100 mM HEPES(NaOH ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were eluted with TN buffer (10 mM Tris-HCl, pH 8.0, 50 mM NaCl) and quantified using the QuantiFluor ONE dsDNA System (Promega). The resulting barcoded DNA samples were combined in approximately equimolar amounts (a total of 100 femtomoles in 10 µl of TN buffer) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysates were diluted 10-fold with 25 mM ammonium bicarbonate and digested with 3 µg of trypsin (Promega, v5111) overnight at 37°C ...
-
bioRxiv - Genetics 2023Quote: ... in Tris 25 mM pH 8.5 in 10% Acetonitrile (ACN) and subjected to overnight trypsin digestion with 0.4 µg of sequencing-grade bovine trypsin (Promega) at 37 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 3 times for 10 min in TBS-T and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10,000; Promega) in 5% non-fat dry milk -TBS-T for 1 h at room temp ...
-
bioRxiv - Bioengineering 2023Quote: ... imaging was performed 10 min after intraperitoneal (i.p.) injection with 200 μL of 15 mg/mL luciferin substrate (Promega). The total flux of luminescence was calculated by gating a region of interest (ROI ...
-
bioRxiv - Genomics 2023Quote: ... 2 µL of yeast lysate was used as a template in a 10 µL GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
-
bioRxiv - Biochemistry 2024Quote: Each sample was rehydrated at 4 °C for 30 min by adding the same volume of 10 ng/µL trypsin in 50 mM ammonium bicarbonate containing 0.01% ProteaseMAX Surfactant (Promega). During rehydration ...
-
bioRxiv - Molecular Biology 2024Quote: ... Total RNAs were collected from a 10-cm dish directly in 400 μl of 1x Reporter lysis buffer (Promega) containing 0.16 U/μl Ribosafe RNase inhibitors (Bioline) ...
-
bioRxiv - Genomics 2023Quote: ... in a final volume of 10 µL starting from 0.03 µg of DNase- treated (RQ1 Rnase-free Dnase supplemented with Rnasin Ribonuclease Inhibitor; Promega) total RNA ...
-
bioRxiv - Microbiology 2023Quote: ... the DNA-coated beads were added to the interaction mixture (BB 1x : 20mM HEPES pH7.9, 100mM KCl, 5mM, 10% Glycerol; supplemented with 160µg acetylated-BSA (Promega R396A), 1.44nmol ds oligoB ...
-
bioRxiv - Bioengineering 2023Quote: ... 250 rpm.10 µl of each culture was collected for the luciferase assay (Promega, Nano-Glo Luciferase Assay System). Cells were diluted in 90 µl media ...
-
bioRxiv - Developmental Biology 2023Quote: ... HEK293 culture medium was replaced by imaging medium containing either Janelia Fluor 646 (JF646) ligand (10 nM; Promega, #GA1120) or OregonGreen ligand (50 nM ...
-
bioRxiv - Developmental Biology 2022Quote: ... into a 10 cm plate of HEK293T cells at 60-70% using Fugene 6 transfection reagent (Promega, Cat #E2691). Media containing lentiviral particles were collected at 24 ...
-
bioRxiv - Immunology 2022Quote: ... The gel pieces were resuspended in 0.1 ml of 100 mM Tris/HCl pH 8.0 containing 10 mM calcium chloride and chymotrypsin (Promega, #V1061) for 16 h at 25 °C ...
-
bioRxiv - Immunology 2022Quote: ... Alkylation was carried out with 10 mM chloroacetamide at room temperature before adding recombinant sequencing-grade trypsin (0.1 μg, Promega). Digestion took place at 37º C for 18 h ...
-
bioRxiv - Systems Biology 2023Quote: ... according to the manufacturer’s instructions and treated with 6.7 μl of DNase buffer and 10 μl of RQ1 RNase-free DNase (Promega), purified again through a p-30 column ...
-
bioRxiv - Cell Biology 2022Quote: ... Three hundred micrograms of each sample were diluted 10 times with 20 mM Tris and digested with 1.5 μl of Mass Spectrometry Grade Trypsin Gold (#V5280, Promega) at 37 °C overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were diluted 3 fold with 20mM HEPES pH 8.0 and digested in 10 @g ml-1 trypsin-TPCK (Promega) overnight at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... or 100-mm dishes were transfected with plasmids (2 μg /dish, 0.4 μg /well, or 10 μg /dish respectively) using FuGENE6 transfection reagent (Promega), according to the manufacturer’s instructions.